##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935613.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684305
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19467196644771	33.0	31.0	34.0	30.0	34.0
2	32.20319740466605	34.0	31.0	34.0	30.0	34.0
3	32.211211375044755	34.0	31.0	34.0	30.0	34.0
4	35.86145213026355	37.0	35.0	37.0	35.0	37.0
5	35.92685717625913	37.0	35.0	37.0	35.0	37.0
6	35.85747291047121	37.0	35.0	37.0	35.0	37.0
7	36.12154960142042	37.0	35.0	37.0	35.0	37.0
8	35.95061120406836	37.0	35.0	37.0	35.0	37.0
9	37.7940245942964	39.0	38.0	39.0	35.0	39.0
10	37.416323130767715	39.0	37.0	39.0	34.0	39.0
11	37.38354096492061	39.0	37.0	39.0	35.0	39.0
12	37.202196389037056	39.0	37.0	39.0	34.0	39.0
13	37.10000365334171	39.0	37.0	39.0	33.0	39.0
14	38.051241770847795	40.0	37.0	41.0	33.0	41.0
15	38.23053024601603	40.0	37.0	41.0	33.0	41.0
16	38.08699483417482	40.0	37.0	41.0	33.0	41.0
17	38.188234778351756	40.0	37.0	41.0	34.0	41.0
18	38.12286480443662	40.0	37.0	41.0	34.0	41.0
19	37.98494677081126	40.0	36.0	41.0	34.0	41.0
20	38.13985138205917	40.0	36.0	41.0	34.0	41.0
21	38.007102096287475	40.0	36.0	41.0	34.0	41.0
22	38.14311893088608	40.0	36.0	41.0	34.0	41.0
23	38.17887637822316	40.0	36.0	41.0	34.0	41.0
24	38.11297155508143	40.0	36.0	41.0	34.0	41.0
25	37.857911311476606	40.0	36.0	41.0	34.0	41.0
26	37.93009257567898	40.0	36.0	41.0	34.0	41.0
27	37.93460810603459	40.0	36.0	41.0	34.0	41.0
28	37.82217286151643	40.0	36.0	41.0	34.0	41.0
29	37.76463857490447	40.0	36.0	41.0	33.0	41.0
30	37.58678513236057	40.0	35.0	41.0	33.0	41.0
31	37.53632371530239	40.0	35.0	41.0	33.0	41.0
32	37.438063436625484	40.0	35.0	41.0	33.0	41.0
33	37.377995192202306	40.0	35.0	41.0	33.0	41.0
34	37.24211864592543	40.0	35.0	41.0	32.0	41.0
35	37.133726920013736	40.0	35.0	41.0	32.0	41.0
36	37.03042941378479	39.0	35.0	41.0	32.0	41.0
37	36.97750710574963	39.0	35.0	41.0	32.0	41.0
38	36.81952784211718	39.0	35.0	41.0	31.0	41.0
39	36.73977100854151	39.0	35.0	41.0	31.0	41.0
40	36.649888573077796	39.0	35.0	41.0	31.0	41.0
41	36.51866930681494	39.0	35.0	41.0	31.0	41.0
42	36.539041801535866	39.0	35.0	41.0	31.0	41.0
43	36.44118631312061	39.0	35.0	41.0	31.0	41.0
44	36.309607558033335	39.0	35.0	41.0	31.0	41.0
45	36.21796567320128	38.0	35.0	41.0	31.0	41.0
46	36.09699622244467	38.0	35.0	41.0	30.0	41.0
47	36.01373510349917	38.0	35.0	40.0	30.0	41.0
48	35.88990581685067	38.0	35.0	40.0	30.0	41.0
49	35.822836308371265	37.0	35.0	40.0	30.0	41.0
50	35.677490300377755	37.0	35.0	40.0	30.0	41.0
51	34.51860354666413	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	18.0
10	28.0
11	19.0
12	17.0
13	18.0
14	27.0
15	40.0
16	70.0
17	137.0
18	196.0
19	374.0
20	596.0
21	973.0
22	1421.0
23	2086.0
24	2986.0
25	4143.0
26	5372.0
27	6287.0
28	7149.0
29	7945.0
30	9628.0
31	12277.0
32	16757.0
33	24401.0
34	47771.0
35	54727.0
36	50071.0
37	75992.0
38	133013.0
39	219632.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39014036138857	19.315656030571162	22.985364713103078	12.3088388949372
2	33.25797707162742	22.939624874873047	24.370565756497466	19.431832297002067
3	33.03673069756907	23.827386910807316	25.911399156808734	17.224483234814887
4	28.72154960142042	25.427550580516	25.797561029073297	20.05333878899029
5	25.428865783532196	29.411008249245583	25.22910105873843	19.931024908483792
6	26.007993511665124	33.50581977334668	25.04994118119844	15.436245533789759
7	79.99576212361447	6.3138512797656015	9.162727146520924	4.527659450099006
8	81.24593565734577	5.738230759675876	8.477067974075887	4.538765608902463
9	75.92579332315269	7.181593003119954	10.754999598132411	6.137614075594946
10	41.58248149582423	26.139952214290414	17.64870927437327	14.62885701551209
11	33.644939025726835	25.582890670095935	22.960083588458364	17.812086715718866
12	28.382811757914965	24.62176953259146	27.480436355134042	19.51498235435953
13	26.800330262090732	25.450639700133713	29.249676679258517	18.499353358517034
14	22.165408699337284	26.837155946544307	30.60682005830733	20.39061529581108
15	20.463097595370485	25.082382855598016	33.67767296746334	20.776846581568158
16	25.23786907884642	25.62249289425037	29.22308035159761	19.9165576753056
17	25.743199304403735	24.086335771330035	27.67201759449368	22.498447329772542
18	27.930089653005602	22.598256625335196	29.116841174622426	20.354812547036776
19	26.895463280262454	25.13689071393604	26.329341448623055	21.63830455717845
20	27.75721352320968	26.542404337247277	26.38165730193408	19.31872483760896
21	25.401684921197422	26.270887981236434	29.084253366554385	19.243173731011755
22	25.003324540957617	23.13734372830828	29.144460437962604	22.714871292771498
23	22.94824676131257	26.614009834795887	28.69831434813424	21.739429055757302
24	23.02248266489358	25.5861056108022	29.509210074455105	21.88220164984912
25	24.668240039163823	26.335186795361714	27.145644120677183	21.850929044797276
26	23.20646495349296	26.05110294386275	27.057963919597256	23.684468183047034
27	23.45313858586449	25.539488970561376	28.373897604138502	22.63347483943563
28	21.26171809354016	26.41804458538225	28.654474247594276	23.665763073483316
29	23.558208693491935	26.749037344458976	27.884495948444044	21.808258013605045
30	24.917397943899285	24.26140390615296	28.589737032463596	22.231461117484162
31	26.505286385456778	25.337970641746004	26.986358422048646	21.17038455074857
32	26.612402364442755	25.122277347089383	27.368497965088668	20.896822323379194
33	26.088513162990186	25.010777358049406	25.68343063400092	23.217278844959484
34	22.893592769306085	26.35155376622997	29.152205522391334	21.602647942072615
35	22.792760538064165	27.547219441623255	28.287240338737845	21.372779681574734
36	25.59691950226873	28.25056078795274	25.122569614426315	21.029950095352216
37	23.672046821227376	27.956978248003452	26.318819824493467	22.052155106275713
38	23.415143832063187	28.441411358970047	26.478690057795866	21.664754751170896
39	22.553978123789832	25.918121305558195	26.593551121210574	24.934349449441402
40	25.897954859309813	24.442755788719943	27.544442901922388	22.11484645004786
41	21.968566647912844	25.413814015680142	27.120362996032473	25.49725634037454
42	24.17416210607843	25.32934875530648	27.62320894922586	22.873280189389234
43	23.50180109746385	24.66926297484309	28.381496554898767	23.447439372794296
44	24.470082784723186	24.465552641000723	27.67435573318915	23.390008841086942
45	23.33754685410745	24.79493792972432	27.129569417145866	24.737945799022366
46	23.04454884883203	25.03255127465092	27.15806548249684	24.76483439402021
47	21.67045396424109	27.450332819429935	28.751068602450662	22.128144613878316
48	22.596941422318995	27.094789604050824	28.014993314384668	22.293275659245513
49	24.95232389066279	24.989295708784827	28.134384521521838	21.92399587903055
50	21.799636127165517	26.50689385580991	28.157473641139553	23.53599637588502
51	22.15883268425629	26.01719993277851	26.227778549038806	25.59618883392639
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	702.0
2	1131.0
3	2494.5
4	3858.0
5	2616.0
6	1374.0
7	1401.5
8	1429.0
9	1427.0
10	1425.0
11	1531.0
12	1637.0
13	1603.5
14	1570.0
15	1604.0
16	1638.0
17	1634.0
18	1630.0
19	1760.0
20	1890.0
21	2340.5
22	2791.0
23	3123.5
24	3456.0
25	4100.0
26	5009.5
27	5275.0
28	6203.0
29	7131.0
30	8511.0
31	9891.0
32	11816.0
33	13741.0
34	14912.0
35	16083.0
36	17782.0
37	19481.0
38	22055.0
39	24629.0
40	26882.5
41	29136.0
42	32008.0
43	34880.0
44	37964.0
45	41048.0
46	45069.0
47	49090.0
48	52977.0
49	56864.0
50	57529.5
51	58195.0
52	55016.5
53	51838.0
54	49824.5
55	47811.0
56	44845.5
57	41880.0
58	38740.0
59	35600.0
60	32327.0
61	29054.0
62	26329.0
63	23604.0
64	20558.0
65	17512.0
66	15290.0
67	13068.0
68	11632.5
69	10197.0
70	8597.0
71	6997.0
72	6039.0
73	5081.0
74	4130.5
75	2752.0
76	2324.0
77	1676.0
78	1028.0
79	704.5
80	381.0
81	331.5
82	282.0
83	187.0
84	92.0
85	68.5
86	45.0
87	29.0
88	13.0
89	11.0
90	9.0
91	9.0
92	9.0
93	8.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.974845164329732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20551575357763	20.479129683812353
2	9.163387922481112	5.126887166241964
3	3.3648200987615806	2.8239096380603965
4	1.8338117108757217	2.052023946891316
5	1.199815179245434	1.6782321932601767
6	0.8455892084880012	1.4193136308047982
7	0.7002361613937788	1.3712298735418893
8	0.5864023672556988	1.3123612342379674
9	0.5394003719135622	1.3580677697277395
>10	8.217345183502285	53.9121099922983
>50	0.29802746892053394	5.028637529036084
>100	0.03935221860744501	1.967185269976129
>500	0.0052469624809926676	0.9906382172115277
>1k	0.0010493924961985336	0.4802738548994101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2193	0.32047113494713614	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1079	0.1576782282754035	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	995	0.14540300012421362	Illumina PCR Primer Index 7 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	926	0.13531977700002193	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	698	0.10200130058964935	Illumina PCR Primer Index 7 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3840100539963906E-4	0.0	0.0	0.10726211265444502	0.0
2	4.3840100539963906E-4	0.0	0.0	0.5098603692797802	0.0
3	4.3840100539963906E-4	0.0	0.0	0.7210235202139397	0.0
4	4.3840100539963906E-4	0.0	0.0	0.9641899445422728	0.0
5	4.3840100539963906E-4	0.0	0.0	1.636404819488386	0.0
6	4.3840100539963906E-4	0.0	0.0	2.145972921431233	0.0
7	4.3840100539963906E-4	0.0	0.0	2.608339848459386	0.0
8	4.3840100539963906E-4	0.0	0.0	3.3300940370156584	0.0
9	4.3840100539963906E-4	0.0	0.0	3.674238826254375	0.0
10	4.3840100539963906E-4	0.0	0.0	4.4158671937220975	0.0
11	4.3840100539963906E-4	0.0	0.0	5.257304856752471	0.0
12	4.3840100539963906E-4	0.0	0.0	5.923820518628389	0.0
13	4.3840100539963906E-4	0.0	0.0	6.22295613797941	0.0
14	4.3840100539963906E-4	0.0	0.0	6.371427945141421	0.0
15	4.3840100539963906E-4	0.0	0.0	6.602026873981631	0.0
16	4.3840100539963906E-4	0.0	0.0	7.010178210008695	0.0
17	4.3840100539963906E-4	0.0	0.0	7.488912107905101	0.0
18	4.3840100539963906E-4	0.0	0.0	8.055910741555302	0.0
19	4.3840100539963906E-4	0.0	0.0	8.385003762941963	0.0
20	4.3840100539963906E-4	0.0	0.0	8.729733086854546	0.0
21	4.3840100539963906E-4	0.0	0.0	9.140660962582475	0.0
22	4.3840100539963906E-4	0.0	0.0	9.574677957928117	0.0
23	4.3840100539963906E-4	0.0	0.0	9.988236239688444	0.0
24	4.3840100539963906E-4	0.0	0.0	10.318206063085904	0.0
25	4.3840100539963906E-4	0.0	0.0	10.632247316620512	0.0
26	4.3840100539963906E-4	0.0	0.0	10.923930118879738	0.0
27	4.3840100539963906E-4	0.0	0.0	11.292917631757769	0.0
28	5.845346738661855E-4	0.0	0.0	11.619380247112034	0.0
29	5.845346738661855E-4	0.0	0.0	11.975069596159607	0.0
30	5.845346738661855E-4	0.0	0.0	12.455264830740678	0.0
31	5.845346738661855E-4	0.0	0.0	12.807593105413522	0.0
32	5.845346738661855E-4	0.0	0.0	13.156121904706234	0.0
33	5.845346738661855E-4	0.0	0.0	13.526132353263531	0.0
34	5.845346738661855E-4	0.0	0.0	13.89891934152169	0.0
35	5.845346738661855E-4	0.0	0.0	14.30297893483169	0.0
36	5.845346738661855E-4	0.0	0.0	14.644639451706476	0.0
37	5.845346738661855E-4	0.0	0.0	15.021810450018632	0.0
38	7.306683423327318E-4	0.0	0.0	15.431861523735762	0.0
39	7.306683423327318E-4	0.0	0.0	16.152592776612767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	20	7.0312835E-4	45.0	42
ATAGCCG	25	3.889145E-5	45.0	1
TCGATTG	25	3.889145E-5	45.0	35
CGTTAAT	20	7.0312835E-4	45.0	30
TATAGCG	25	3.889145E-5	45.0	1
CTCGAAC	20	7.0312835E-4	45.0	13
ACCGTAA	20	7.0312835E-4	45.0	27
CACGTTG	25	3.889145E-5	45.0	23
TACGAAT	90	0.0	42.5	12
CGAATAT	90	0.0	42.5	14
TAGCCGT	60	3.6379788E-12	41.250004	44
CGTAAGG	105	0.0	40.714287	2
GCGTACG	45	1.9263098E-8	40.0	1
AACACGT	305	0.0	39.836067	41
ACGGGTA	80	0.0	39.375	5
TTAAGCG	40	3.4554796E-7	39.375	1
ACAACGA	405	0.0	38.88889	13
CAATTGG	105	0.0	38.57143	2
ACGGGAC	210	0.0	38.57143	5
CGTTTTT	1110	0.0	38.51351	1
>>END_MODULE