FastQCFastQC Report
Sat 14 Jan 2017
SRR2935611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721753
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68590.9503251112222602No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC52100.7218535981145905No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC29250.40526329644629117No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG27960.3873901459363522No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC16970.2351219877160192TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT16610.23013413175975717No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT12470.17277378826274362No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC11430.15836442661131994No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC9330.12926860019979136TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC9110.12622046600429787No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT7650.10599193907056847No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT7530.10432932041848113TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG7480.10363656264677805TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG253.889357E-545.0000041
TAACGCG253.889357E-545.0000041
TACACGG502.1827873E-1145.0000042
CGAACTA207.031541E-445.019
TATCTCG207.031541E-445.013
CGTTTTT31100.042.106111
TATAGCG451.9266736E-840.01
AGTACGG800.039.3752
GTTACCG356.24506E-638.57142638
TAGCCGT900.037.544
GTACCGG301.1395014E-437.4999962
AATTCGT301.1395014E-437.49999631
ATAACGC301.1395014E-437.49999611
TGCGTAG301.1395014E-437.4999961
CTACGGG1700.037.0588233
AAACGGG1100.036.818183
ATAGGGA4650.036.2903254
CGTATGG504.8747097E-836.0000042
ACGCACC250.002106589736.00000432
TTGCGTG250.002106589736.0000041
Produced by FastQC (version 0.11.5)