##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721753
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09134426874568	33.0	31.0	34.0	30.0	34.0
2	32.13064164610331	33.0	31.0	34.0	30.0	34.0
3	32.101329679267	33.0	31.0	34.0	30.0	34.0
4	35.77580280234374	37.0	35.0	37.0	35.0	37.0
5	35.84671071682418	37.0	35.0	37.0	35.0	37.0
6	35.777969748653625	37.0	35.0	37.0	35.0	37.0
7	36.04872442511496	37.0	35.0	37.0	35.0	37.0
8	35.874027541277975	37.0	35.0	37.0	35.0	37.0
9	37.72505968108203	39.0	38.0	39.0	35.0	39.0
10	37.376685652847996	39.0	37.0	39.0	34.0	39.0
11	37.33372358687806	39.0	37.0	39.0	34.0	39.0
12	37.19993404946013	39.0	37.0	39.0	34.0	39.0
13	37.14394536635109	39.0	37.0	39.0	34.0	39.0
14	38.18861715850159	40.0	37.0	41.0	33.0	41.0
15	38.29266521926476	40.0	38.0	41.0	33.0	41.0
16	38.127687726964766	40.0	37.0	41.0	33.0	41.0
17	38.17702177891883	40.0	37.0	41.0	33.0	41.0
18	38.059609035223964	40.0	37.0	41.0	33.0	41.0
19	37.87845149240807	40.0	37.0	41.0	33.0	41.0
20	37.98558925283303	40.0	36.0	41.0	34.0	41.0
21	37.90991932142991	40.0	36.0	41.0	33.0	41.0
22	38.032960029262085	40.0	36.0	41.0	34.0	41.0
23	38.05515044620528	40.0	36.0	41.0	34.0	41.0
24	37.97176596425647	40.0	36.0	41.0	34.0	41.0
25	37.73067794661054	40.0	35.0	41.0	33.0	41.0
26	37.80592529577293	40.0	35.0	41.0	34.0	41.0
27	37.81162807774959	40.0	35.0	41.0	34.0	41.0
28	37.68225833491513	40.0	35.0	41.0	33.0	41.0
29	37.6395359631342	40.0	35.0	41.0	33.0	41.0
30	37.43689184527117	40.0	35.0	41.0	33.0	41.0
31	37.3473099522967	40.0	35.0	41.0	33.0	41.0
32	37.20289351066085	40.0	35.0	41.0	32.0	41.0
33	37.04878538779887	40.0	35.0	41.0	32.0	41.0
34	36.88067247382415	40.0	35.0	41.0	31.0	41.0
35	36.74960270341793	39.0	35.0	41.0	31.0	41.0
36	36.64223494741276	39.0	35.0	41.0	31.0	41.0
37	36.618520463371816	39.0	35.0	41.0	31.0	41.0
38	36.470181627232584	39.0	35.0	41.0	31.0	41.0
39	36.39793669025276	39.0	35.0	41.0	30.0	41.0
40	36.28633479874694	39.0	35.0	41.0	30.0	41.0
41	36.138350654586816	39.0	35.0	41.0	30.0	41.0
42	36.142095703100644	39.0	35.0	41.0	30.0	41.0
43	36.08619015092421	39.0	35.0	41.0	30.0	41.0
44	35.96069153851803	39.0	35.0	41.0	29.0	41.0
45	35.91866885208652	38.0	35.0	41.0	29.0	41.0
46	35.79291946136698	38.0	35.0	40.0	29.0	41.0
47	35.68940620960356	38.0	35.0	40.0	29.0	41.0
48	35.59625799962037	38.0	35.0	40.0	29.0	41.0
49	35.54742688980856	38.0	35.0	40.0	28.0	41.0
50	35.41263008259058	37.0	35.0	40.0	28.0	41.0
51	34.33846343555205	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	12.0
10	16.0
11	18.0
12	17.0
13	18.0
14	23.0
15	47.0
16	69.0
17	132.0
18	249.0
19	475.0
20	719.0
21	1191.0
22	1812.0
23	2525.0
24	3580.0
25	5583.0
26	7846.0
27	9005.0
28	9585.0
29	10199.0
30	11282.0
31	13740.0
32	17906.0
33	24938.0
34	47272.0
35	56500.0
36	53071.0
37	78241.0
38	136668.0
39	228860.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51052645434103	19.00567091511916	22.871120729667908	15.612681900871905
2	33.6629012972582	24.962417890885106	23.484349909179457	17.89033090267723
3	30.648573681023837	24.950779560320495	28.05738251174571	16.343264246909953
4	26.725486419869398	23.597546529075736	30.305935687139506	19.371031363915357
5	25.570243559777378	27.89596995093889	26.798641640561243	19.735144848722484
6	24.78188521557929	33.00367300170557	27.07574474924247	15.13869703347267
7	76.87519137433443	5.59748279536074	12.873102016894977	4.654223813409851
8	77.95935728704973	6.003715952687416	11.318137922530285	4.718788837732576
9	72.55834059574397	6.706865090966023	12.966208661411867	7.768585651878136
10	40.78500539658304	25.884617036576223	19.29025580773478	14.040121759105956
11	30.973338524398237	23.85622228102966	26.651499889851515	18.51893930472059
12	27.1321352318591	21.438774760894656	31.01268716583097	20.416402841415277
13	25.58991788049374	22.4166716314307	32.73737691426291	19.256033573812648
14	21.445563787057345	25.173986114363224	32.38379334758567	20.99665675099376
15	19.710482672049856	23.91760061960255	35.54498561142108	20.82693109692651
16	24.448530175835778	24.522516705853665	30.839774826013887	20.189178292296674
17	24.552305290036898	23.76297708495843	29.861185197706142	21.823532427298538
18	25.580496374798578	23.251167643224203	29.8293183402078	21.339017641769413
19	24.050471560215197	25.59726111287379	28.42911633204157	21.923150994869438
20	26.369547476768368	26.19732789472299	28.22669251114994	19.20643211735871
21	24.465572017019674	26.689601567295185	29.903027767117006	18.941798648568138
22	24.8562874002602	22.139845625858154	30.352350457843613	22.651516516038033
23	22.818332587464134	25.43986654714286	29.91965395363788	21.82214691175513
24	23.1545972098488	23.67222581686533	30.617538132851546	22.55563884043433
25	23.484072806070774	26.091474507206758	27.56427752984747	22.860175156875
26	22.28172241750294	24.86751007616179	27.929499427089322	24.921268079245948
27	23.168868019945883	24.23211264795574	28.437013770638984	24.16200556145939
28	20.791461899015314	25.826148280644485	28.773832599241018	24.608557221099183
29	23.613479957824907	25.05968108203222	27.418521294681142	23.90831766546173
30	25.085728774248256	23.521481725742742	29.102753989245628	22.290035510763378
31	26.90158544543632	23.079502267396187	27.787068429227173	22.23184385794032
32	26.72825745095621	24.981676556938453	27.090846868665597	21.199219123439736
33	25.510250736747892	23.34302732375203	27.648101220223538	23.498620719276538
34	22.893566081471086	24.135957869243356	30.98082030833263	21.98965574095293
35	22.132640945032442	27.18991123001913	28.333931414209566	22.343516410738854
36	23.29522703750452	27.583259092792133	26.249838933818083	22.87167493588527
37	22.952034837402824	27.228705665234504	26.88800739311094	22.931252104251733
38	22.632950607756396	27.524513233751712	27.19877852949693	22.643757628994962
39	22.57766853757449	25.280255156542474	27.889458027884885	24.252618277998153
40	23.3899963006735	23.88102300925663	29.95637011553814	22.77261057453173
41	20.95536838780026	24.76359641040633	28.857656289617083	25.423378912176325
42	22.54607878318483	24.93290640981056	28.647612133236716	23.873402673767895
43	23.22539705411685	23.671255955984943	28.098532323384866	25.004814666513337
44	23.391381816216906	23.071743380353112	28.44920630742096	25.087668496009023
45	23.47853074389715	22.79505592633491	27.632583446137392	26.093829883630548
46	23.204891424074443	23.98743060299022	27.552084993065495	25.255592979869846
47	20.802130368699544	24.760686827765177	30.567105367071562	23.87007743646372
48	21.92606057751059	25.322651932170704	28.522361528112803	24.228925962205906
49	24.077766216420297	23.431977421638706	29.49721026445335	22.993046097487646
50	21.8285202832548	23.704508329026687	29.22440225395669	25.242569133761826
51	21.174279843658425	23.803849793488908	28.0457441811811	26.976126181671567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	860.0
2	1331.0
3	6058.0
4	10785.0
5	7031.0
6	3277.0
7	3113.0
8	2949.0
9	2942.0
10	2935.0
11	2943.0
12	2951.0
13	2892.5
14	2834.0
15	2713.0
16	2592.0
17	2545.5
18	2499.0
19	2463.0
20	2427.0
21	2622.0
22	2817.0
23	2999.5
24	3182.0
25	3799.0
26	5145.5
27	5875.0
28	6365.0
29	6855.0
30	7939.5
31	9024.0
32	10918.5
33	12813.0
34	14110.0
35	15407.0
36	17722.0
37	20037.0
38	21389.5
39	22742.0
40	24389.5
41	26037.0
42	28597.5
43	31158.0
44	33876.5
45	36595.0
46	50084.0
47	63573.0
48	61123.5
49	58674.0
50	59285.0
51	59896.0
52	54847.0
53	49798.0
54	46739.0
55	43680.0
56	41357.5
57	39035.0
58	38185.5
59	37336.0
60	36067.5
61	34799.0
62	33008.0
63	31217.0
64	27421.0
65	23625.0
66	19904.5
67	16184.0
68	13376.0
69	10568.0
70	9114.0
71	7660.0
72	6339.5
73	5019.0
74	4492.0
75	3139.0
76	2313.0
77	1833.0
78	1353.0
79	1003.0
80	653.0
81	470.0
82	287.0
83	199.0
84	111.0
85	72.5
86	34.0
87	31.0
88	28.0
89	19.0
90	10.0
91	8.0
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.221784238930866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57820440137856	23.385992426702423
2	8.91863509871502	5.747486717131026
3	3.5257866637732027	3.408214114578001
4	2.072108392962174	2.670681182308198
5	1.5092708799609615	2.4315700326101712
6	1.182107536756158	2.285376839794258
7	0.9794719225481088	2.2092233069505176
8	0.8464967415430183	2.182050829196573
9	0.7146179334913672	2.0723638379606535
>10	7.560097035115806	45.9702985947947
>50	0.0760450278998632	1.5908893270685756
>100	0.02938103346734185	1.7834332946044358
>500	0.004320740215785567	0.985413025699469
>1k	0.00259244412947134	1.5967366476048614
>5k	8.641480431571134E-4	1.6802698229961701
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6859	0.9503251112222602	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	5210	0.7218535981145905	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	2925	0.40526329644629117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	2796	0.3873901459363522	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1697	0.2351219877160192	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT	1661	0.23013413175975717	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT	1247	0.17277378826274362	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	1143	0.15836442661131994	No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	933	0.12926860019979136	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	911	0.12622046600429787	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT	765	0.10599193907056847	No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	753	0.10432932041848113	TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	748	0.10363656264677805	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.771031086812247E-4	0.0	0.0	0.10890152171172132	0.0
2	2.771031086812247E-4	0.0	0.0	0.5683384759051919	0.0
3	2.771031086812247E-4	0.0	0.0	0.8565257089336656	0.0
4	2.771031086812247E-4	0.0	0.0	1.4208461897629798	0.0
5	2.771031086812247E-4	0.0	0.0	2.9676357424215762	0.0
6	2.771031086812247E-4	0.0	0.0	3.951074675131243	0.0
7	2.771031086812247E-4	0.0	0.0	4.768390294186515	0.0
8	2.771031086812247E-4	0.0	0.0	5.852833310010489	0.0
9	2.771031086812247E-4	0.0	0.0	6.377250943189706	0.0
10	2.771031086812247E-4	0.0	0.0	7.587914425017977	0.0
11	2.771031086812247E-4	0.0	0.0	8.551540485456936	0.0
12	2.771031086812247E-4	0.0	0.0	9.669651528985678	0.0
13	2.771031086812247E-4	0.0	0.0	10.046511756792144	0.0
14	2.771031086812247E-4	0.0	0.0	10.259049841150643	0.0
15	2.771031086812247E-4	0.0	0.0	10.554026100341806	0.0
16	2.771031086812247E-4	0.0	0.0	10.965385665179085	0.0
17	2.771031086812247E-4	0.0	0.0	11.413184288807944	0.0
18	2.771031086812247E-4	0.0	0.0	11.922222699455354	0.0
19	2.771031086812247E-4	0.0	0.0	12.338293017140213	0.0
20	2.771031086812247E-4	0.0	0.0	12.687165830969874	0.0
21	2.771031086812247E-4	0.0	0.0	13.094091746068253	0.0
22	2.771031086812247E-4	0.0	0.0	13.569462129010894	0.0
23	2.771031086812247E-4	0.0	0.0	13.982345760945918	0.0
24	2.771031086812247E-4	0.0	0.0	14.327477682808384	0.0
25	2.771031086812247E-4	0.0	0.0	14.647393221780858	0.0
26	2.771031086812247E-4	0.0	0.0	14.965784693655586	0.0
27	2.771031086812247E-4	0.0	0.0	15.318398399452445	0.0
28	2.771031086812247E-4	0.0	0.0	15.652584748522	0.0
29	2.771031086812247E-4	0.0	0.0	16.03069194031753	0.0
30	2.771031086812247E-4	0.0	0.0	16.488743378967598	0.0
31	2.771031086812247E-4	0.0	0.0	16.849115971807528	0.0
32	4.156546630218371E-4	0.0	0.0	17.238722942613332	0.0
33	4.156546630218371E-4	0.0	0.0	17.592722163953596	0.0
34	4.156546630218371E-4	0.0	0.0	17.974985902379345	0.0
35	4.156546630218371E-4	0.0	0.0	18.36958072914141	0.0
36	4.156546630218371E-4	0.0	0.0	18.734941177937603	0.0
37	4.156546630218371E-4	0.0	0.0	19.122746978536977	0.0
38	4.156546630218371E-4	0.0	0.0	19.55669044673178	0.0
39	4.156546630218371E-4	0.0	0.0	20.196244421568043	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	25	3.889357E-5	45.000004	1
TAACGCG	25	3.889357E-5	45.000004	1
TACACGG	50	2.1827873E-11	45.000004	2
CGAACTA	20	7.031541E-4	45.0	19
TATCTCG	20	7.031541E-4	45.0	13
CGTTTTT	3110	0.0	42.10611	1
TATAGCG	45	1.9266736E-8	40.0	1
AGTACGG	80	0.0	39.375	2
GTTACCG	35	6.24506E-6	38.571426	38
TAGCCGT	90	0.0	37.5	44
GTACCGG	30	1.1395014E-4	37.499996	2
AATTCGT	30	1.1395014E-4	37.499996	31
ATAACGC	30	1.1395014E-4	37.499996	11
TGCGTAG	30	1.1395014E-4	37.499996	1
CTACGGG	170	0.0	37.058823	3
AAACGGG	110	0.0	36.81818	3
ATAGGGA	465	0.0	36.290325	4
CGTATGG	50	4.8747097E-8	36.000004	2
ACGCACC	25	0.0021065897	36.000004	32
TTGCGTG	25	0.0021065897	36.000004	1
>>END_MODULE