##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935610.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595758
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.119677788632295	33.0	31.0	34.0	30.0	34.0
2	32.15440665505121	33.0	31.0	34.0	30.0	34.0
3	32.12103907962629	33.0	31.0	34.0	30.0	34.0
4	35.805644238096676	37.0	35.0	37.0	35.0	37.0
5	35.8794544093407	37.0	35.0	37.0	35.0	37.0
6	35.83150708844867	37.0	35.0	37.0	35.0	37.0
7	36.110581813420886	37.0	35.0	37.0	35.0	37.0
8	35.948834929619075	37.0	35.0	37.0	35.0	37.0
9	37.81829031250944	39.0	38.0	39.0	35.0	39.0
10	37.38061091919874	39.0	37.0	39.0	34.0	39.0
11	37.33086588849835	39.0	37.0	39.0	34.0	39.0
12	37.3138824824845	39.0	37.0	39.0	34.0	39.0
13	37.280496443186664	39.0	37.0	39.0	34.0	39.0
14	38.48119202763538	40.0	38.0	41.0	34.0	41.0
15	38.56439695312526	40.0	38.0	41.0	34.0	41.0
16	38.38912780021418	40.0	38.0	41.0	34.0	41.0
17	38.42126501028941	40.0	38.0	41.0	34.0	41.0
18	38.32044051443707	40.0	38.0	41.0	34.0	41.0
19	38.13739471396104	40.0	37.0	41.0	34.0	41.0
20	38.21801805431064	40.0	37.0	41.0	34.0	41.0
21	38.19910433431024	40.0	37.0	41.0	34.0	41.0
22	38.29142537741835	40.0	37.0	41.0	34.0	41.0
23	38.287405288724614	40.0	37.0	41.0	34.0	41.0
24	38.22747155724304	40.0	37.0	41.0	34.0	41.0
25	38.00576576395113	40.0	36.0	41.0	34.0	41.0
26	38.08150792771561	40.0	37.0	41.0	34.0	41.0
27	38.07227095565649	40.0	37.0	41.0	34.0	41.0
28	37.95680293004878	40.0	36.0	41.0	34.0	41.0
29	37.90350108601143	40.0	36.0	41.0	34.0	41.0
30	37.762836588010565	40.0	36.0	41.0	33.0	41.0
31	37.71477848388104	40.0	36.0	41.0	33.0	41.0
32	37.60032261421584	40.0	36.0	41.0	33.0	41.0
33	37.43173234769823	40.0	36.0	41.0	33.0	41.0
34	37.27262411918934	40.0	36.0	41.0	32.0	41.0
35	37.179990868775576	40.0	36.0	41.0	32.0	41.0
36	37.08965049567106	40.0	36.0	41.0	32.0	41.0
37	37.06404110393817	40.0	36.0	41.0	32.0	41.0
38	36.92797914589481	40.0	35.0	41.0	31.0	41.0
39	36.85432675683751	40.0	35.0	41.0	31.0	41.0
40	36.79481265883127	40.0	35.0	41.0	31.0	41.0
41	36.6424538151397	40.0	35.0	41.0	30.0	41.0
42	36.67315923579709	40.0	35.0	41.0	31.0	41.0
43	36.59329291423699	40.0	35.0	41.0	30.0	41.0
44	36.508911336482264	39.0	35.0	41.0	30.0	41.0
45	36.48790616324078	39.0	35.0	41.0	30.0	41.0
46	36.375164076688854	39.0	35.0	41.0	30.0	41.0
47	36.294478630584905	39.0	35.0	41.0	30.0	41.0
48	36.189719651267794	39.0	35.0	41.0	29.0	41.0
49	36.16133228592818	39.0	35.0	41.0	29.0	41.0
50	36.036956280906004	39.0	35.0	41.0	29.0	41.0
51	34.93494002598371	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	11.0
11	14.0
12	13.0
13	11.0
14	5.0
15	16.0
16	34.0
17	58.0
18	151.0
19	289.0
20	548.0
21	922.0
22	1521.0
23	2241.0
24	3121.0
25	4666.0
26	6259.0
27	7063.0
28	7066.0
29	7086.0
30	8109.0
31	9861.0
32	12551.0
33	17186.0
34	30650.0
35	43292.0
36	37704.0
37	57046.0
38	110546.0
39	227557.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40012891140362	20.47576364899842	24.823334306882998	14.300773132714962
2	32.44891382071244	26.540474487963234	25.446070384283555	15.564541307040777
3	29.06901795695568	25.85059705450871	29.163351562211503	15.917033426324112
4	26.232799223845927	24.438278629913487	31.52588802836051	17.803034117880078
5	26.682814162797648	28.33113445392257	26.85721383514783	18.12883754813196
6	23.776936272781903	35.08488345939123	26.63077961185582	14.507400655971047
7	79.11551334602305	4.622346657535442	12.444818197993145	3.8173217984483636
8	81.27863998469176	5.964334511664132	9.27524263207544	3.4817828715686567
9	75.59344566082201	6.802930048778196	10.62444818197993	6.979176108419861
10	36.46581330003122	33.28146663578164	17.907942486714404	12.344777577472732
11	25.544600324292748	25.0141836114664	32.333598541689746	17.107617522551102
12	23.643157120844368	21.834033281970196	33.999879145559106	20.52293045162633
13	22.204989274168373	24.14151383615495	35.9701422389628	17.68335465071388
14	18.711288811900133	29.082781934946738	32.57161464890107	19.63431460425206
15	17.582306909852658	25.909010034275664	37.37692150168357	19.13176155418811
16	20.59141463480138	26.204767707693392	32.844544261260445	20.359273396244784
17	21.00769104233598	25.918745530903486	31.41157986967863	21.6619835570819
18	21.40567143034588	25.29852725435496	32.312113307752476	20.98368800754669
19	21.25728903346661	27.42556541414467	30.458844027272818	20.858301525115902
20	23.565105294431632	27.184527945910926	31.212673602368746	18.037693157288697
21	22.395167165191236	28.081368609401803	31.101051097929023	18.422413127477935
22	21.534750687359633	23.81789249997482	31.69340571171516	22.953951100950388
23	19.95692882009138	27.90579396332068	31.286361240637977	20.850915975949967
24	20.497416736325825	25.983369086105434	32.21828326266706	21.300930914901688
25	19.29827211720195	29.639215923244	30.306768855810578	20.755743103743466
26	18.778430167954102	28.275239275007635	30.990939274000517	21.95539128303774
27	20.536358722837125	27.731058584190226	30.554352606259588	21.17823008671306
28	18.911202199550825	26.87601341484294	33.521161276894304	20.691623108711926
29	20.20568754427133	26.43875533354147	31.465964368082343	21.889592754104857
30	21.09363197808506	27.355906257238676	30.32103639397205	21.22942537070421
31	23.292679242242656	26.424152088599733	28.57754323064063	21.705625438516982
32	23.54664142151679	27.953464326118993	28.242843570711596	20.257050681652615
33	21.438906401592593	26.234813464527544	28.51140899492747	23.81487113895239
34	20.88381524041641	27.245794433310174	30.22619922854582	21.6441910977276
35	20.215087334118888	26.75666965445701	30.15502939112861	22.873213620295488
36	21.760009936920696	28.935238805018145	28.602889092551003	20.701862165510157
37	21.41624619392438	27.1185615635879	29.874042816042756	21.591149426444968
38	21.34910483787041	27.66777785610936	27.84352035558062	23.139596950439607
39	22.111662789253355	26.82313959695044	29.07657135951175	21.98862625428446
40	20.929303509142976	25.085689155663875	31.072012461435683	22.912994873757462
41	18.9048237707257	27.62715733569671	30.0376998714243	23.43031902215329
42	20.679873371402483	26.9950214684486	30.426280469586644	21.898824690562275
43	21.370757925197815	27.13182198140856	28.007009557572033	23.490410535821592
44	21.654933714696238	25.998811597997847	28.90922152954723	23.437033157758687
45	21.039583186461616	25.505490484391313	28.830498289573953	24.624428039573115
46	22.049557034903433	26.883231110618745	28.164623890908725	22.9025879635691
47	19.702630934036975	25.59545990150363	31.779178794074102	22.922730370385292
48	20.67366279596749	25.94258071230265	30.55032412489635	22.833432366833513
49	20.722172425716483	24.567190033537106	31.89029773834342	22.82033980240299
50	21.163291134991052	24.76273923304429	30.63794359454678	23.43602603741788
51	20.59074322124084	24.594382282738962	28.70444039358263	26.11043410243757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	473.5
2	700.0
3	3878.0
4	7056.0
5	4755.5
6	2455.0
7	2423.0
8	2391.0
9	2420.0
10	2449.0
11	2485.5
12	2522.0
13	2413.0
14	2304.0
15	2299.0
16	2294.0
17	2294.5
18	2295.0
19	2327.0
20	2359.0
21	2505.5
22	2652.0
23	2967.5
24	3283.0
25	3934.5
26	6017.0
27	7448.0
28	8127.0
29	8806.0
30	10804.5
31	12803.0
32	13579.0
33	14355.0
34	17491.0
35	20627.0
36	21564.5
37	22502.0
38	24275.0
39	26048.0
40	28211.0
41	30374.0
42	33306.5
43	36239.0
44	39097.5
45	41956.0
46	53310.0
47	64664.0
48	60560.5
49	56457.0
50	53679.0
51	50901.0
52	45005.5
53	39110.0
54	34769.0
55	30428.0
56	27555.5
57	24683.0
58	22651.0
59	20619.0
60	18726.5
61	16834.0
62	14439.0
63	12044.0
64	9968.0
65	7892.0
66	6242.5
67	4593.0
68	3884.0
69	3175.0
70	2607.0
71	2039.0
72	1720.5
73	1402.0
74	1279.0
75	861.0
76	566.0
77	404.5
78	243.0
79	184.5
80	126.0
81	86.0
82	46.0
83	30.0
84	14.0
85	10.5
86	7.0
87	5.0
88	3.0
89	1.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.83034577468451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92835227491538	24.59923713512841
2	9.193727656923139	6.036665158701335
3	3.2937197096690776	3.244018708599879
4	1.7840014208239094	2.342775340327096
5	1.2007717965623885	1.9710876638816168
6	0.912894654164663	1.798238829125215
7	0.7699450717757774	1.7694294053739141
8	0.612617352659461	1.6089951612305555
9	0.5414171391738282	1.599742069867554
>10	6.539989129616021	45.781334348283444
>50	0.19018153273680619	3.7926413555795784
>100	0.024672198841517647	1.4174944332303911
>500	0.004626037282784559	1.0022023141613445
>1k	0.003084024855189706	3.0361380765096664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4304	0.722440991140698	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	4267	0.716230415705706	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	3803	0.6383464426831029	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	3235	0.543005717086468	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1351	0.22676993007227766	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1032	0.173224698619238	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	795	0.13344344515726186	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	730	0.12253297479849201	No Hit
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	701	0.1176652264845793	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	692	0.11615454597336501	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGGCTTAT	685	0.11497957224242059	No Hit
GAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	643	0.1079297298567539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.357067802698411E-4	0.0	0.0	0.14838239687926977	0.0
2	3.357067802698411E-4	0.0	0.0	0.7216017241900234	0.0
3	3.357067802698411E-4	0.0	0.0	1.077450911276055	0.0
4	3.357067802698411E-4	0.0	0.0	1.5346835460035786	0.0
5	3.357067802698411E-4	0.0	0.0	2.9144384129126255	0.0
6	3.357067802698411E-4	0.0	0.0	3.6937817033090616	0.0
7	3.357067802698411E-4	0.0	0.0	4.431665206342172	0.0
8	3.357067802698411E-4	0.0	0.0	5.466313503133823	0.0
9	3.357067802698411E-4	0.0	0.0	5.924049698031751	0.0
10	3.357067802698411E-4	0.0	0.0	7.423484032106996	0.0
11	3.357067802698411E-4	0.0	0.0	8.46400719755337	0.0
12	3.357067802698411E-4	0.0	0.0	10.009097653745313	0.0
13	3.357067802698411E-4	0.0	0.0	10.441655840123003	0.0
14	3.357067802698411E-4	0.0	0.0	10.648115509988955	0.0
15	3.357067802698411E-4	0.0	0.0	10.973079673290162	0.0
16	3.357067802698411E-4	0.0	0.0	11.452468955515494	0.0
17	3.357067802698411E-4	0.0	0.0	12.077219273597668	0.0
18	3.357067802698411E-4	0.0	0.0	12.79093188845135	0.0
19	3.357067802698411E-4	0.0	0.0	13.335616139439168	0.0
20	3.357067802698411E-4	0.0	0.0	13.77539202159266	0.0
21	3.357067802698411E-4	0.0	0.0	14.27408444368351	0.0
22	3.357067802698411E-4	0.0	0.0	14.843946703191564	0.0
23	3.357067802698411E-4	0.0	0.0	15.41112330845746	0.0
24	3.357067802698411E-4	0.0	0.0	15.864159608431612	0.0
25	3.357067802698411E-4	0.0	0.0	16.240822615894373	0.0
26	3.357067802698411E-4	0.0	0.0	16.602714525025263	0.0
27	3.357067802698411E-4	0.0	0.0	17.007073341860284	0.0
28	3.357067802698411E-4	0.0	0.0	17.40119310189708	0.0
29	3.357067802698411E-4	0.0	0.0	17.827708566229912	0.0
30	3.357067802698411E-4	0.0	0.0	18.333618684096564	0.0
31	3.357067802698411E-4	0.0	0.0	18.78346576965815	0.0
32	3.357067802698411E-4	0.0	0.0	19.22038814417935	0.0
33	6.714135605396822E-4	0.0	0.0	19.664863921256618	0.0
34	6.714135605396822E-4	0.0	0.0	20.1108503788451	0.0
35	6.714135605396822E-4	0.0	0.0	20.549954847438055	0.0
36	6.714135605396822E-4	0.0	0.0	20.970931149896433	0.0
37	6.714135605396822E-4	0.0	0.0	21.40600713712615	0.0
38	6.714135605396822E-4	0.0	0.0	21.851657887934362	0.0
39	6.714135605396822E-4	0.0	0.0	22.35740015241088	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.030548E-4	45.0	1
GACGTAG	40	6.8048394E-9	45.0	1
GTTAGCG	20	7.030548E-4	45.0	1
CTAAGCG	20	7.030548E-4	45.0	1
TCGATAG	25	3.8885362E-5	45.0	1
TAGCCGT	25	3.8885362E-5	45.0	44
ACGCTAA	20	7.030548E-4	45.0	42
CCGACGG	25	3.8885362E-5	45.0	2
CTCGAAC	25	3.8885362E-5	45.0	12
AATAGCG	20	7.030548E-4	45.0	1
GCCGTTG	25	3.8885362E-5	45.0	1
TCGCGTG	25	3.8885362E-5	45.0	1
TCGGTAG	20	7.030548E-4	45.0	1
TCGAACA	20	7.030548E-4	45.0	13
TATTGCG	75	0.0	42.0	1
CGTTTTT	2340	0.0	41.923073	1
TTAGCGG	140	0.0	41.785713	2
GCGATTT	60	3.6379788E-12	41.249996	9
CTCGATC	55	6.002665E-11	40.909092	16
ATTAGCG	50	1.0804797E-9	40.5	1
>>END_MODULE