FastQCFastQC Report
Sat 14 Jan 2017
SRR2935609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962016
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130671.358293417157303No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGC45890.4770190932375345No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC41760.4340884143299072No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCG40390.41984748694408414No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTT39120.40664604330905096No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC34860.36236403552539664No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCT22600.2349233276785417No Hit
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC21900.2276469414230117No Hit
CGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG20410.21215863353624057No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC20100.20893623390879154No Hit
CGTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCT15020.15613045936865916No Hit
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT14740.15321990486644713Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCC14410.1497896084888401No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG12250.12733675947177592No Hit
GAACTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCT10240.10644313608089678No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTC10220.10623523933073879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA207.0327136E-445.039
ATAACGC253.89033E-545.011
ATACGCG253.89033E-545.01
CAATACG207.0327136E-445.015
TCGCGAC207.0327136E-445.024
CGTTTTT58050.043.2945751
TACGCGG750.042.02
TCGTAAG603.6379788E-1241.2500041
CGTTTCT4350.040.8620721
CGTTCTG4300.040.8139531
TTAAGCG451.927765E-840.01
TCGATAG403.457444E-739.3751
TACGATC403.457444E-739.37511
TATTGCG750.039.01
AATAGCG356.2472245E-638.571431
CGTAAGG1350.038.3333362
CCGATGA2850.037.89473318
TAGCCGT900.037.544
TACGTAG850.037.0588231
TACGGGA3600.036.8754
Produced by FastQC (version 0.11.5)