##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	962016
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06716520307354	33.0	31.0	34.0	30.0	34.0
2	32.11431202807438	33.0	31.0	34.0	30.0	34.0
3	32.039704121345174	33.0	31.0	34.0	30.0	34.0
4	35.75245837907062	37.0	35.0	37.0	33.0	37.0
5	35.836280269766824	37.0	35.0	37.0	35.0	37.0
6	35.78574888567342	37.0	35.0	37.0	35.0	37.0
7	36.044128164188535	37.0	35.0	37.0	35.0	37.0
8	35.8834281342514	37.0	35.0	37.0	35.0	37.0
9	37.70435522901906	39.0	38.0	39.0	35.0	39.0
10	37.36531928782889	39.0	37.0	39.0	34.0	39.0
11	37.32459127498919	39.0	37.0	39.0	34.0	39.0
12	37.200856326713904	39.0	37.0	39.0	34.0	39.0
13	37.13255704686824	39.0	37.0	39.0	33.0	39.0
14	38.133996731863085	40.0	37.0	41.0	33.0	41.0
15	38.28168138575658	40.0	38.0	41.0	33.0	41.0
16	38.143054793267474	40.0	37.0	41.0	33.0	41.0
17	38.19046772610851	40.0	37.0	41.0	33.0	41.0
18	38.053412833050594	40.0	37.0	41.0	33.0	41.0
19	37.829813641353155	39.0	37.0	41.0	33.0	41.0
20	37.858058493829624	40.0	35.0	41.0	33.0	41.0
21	37.781252078967505	40.0	35.0	41.0	33.0	41.0
22	37.908892367694506	40.0	35.0	41.0	34.0	41.0
23	37.94837403951701	40.0	35.0	41.0	34.0	41.0
24	37.88021924791272	40.0	35.0	41.0	34.0	41.0
25	37.62594593021322	39.0	35.0	41.0	33.0	41.0
26	37.71760552839038	40.0	35.0	41.0	34.0	41.0
27	37.71622717293683	40.0	35.0	41.0	34.0	41.0
28	37.56896558892991	40.0	35.0	41.0	33.0	41.0
29	37.46485817283704	40.0	35.0	41.0	33.0	41.0
30	37.25317770182617	39.0	35.0	41.0	33.0	41.0
31	37.17692429231946	39.0	35.0	41.0	33.0	41.0
32	37.018936275488144	39.0	35.0	41.0	32.0	41.0
33	36.77718457905066	39.0	35.0	41.0	31.0	41.0
34	36.50146775105612	39.0	35.0	41.0	30.0	41.0
35	36.34695368891993	39.0	35.0	41.0	30.0	41.0
36	36.164122010444736	39.0	35.0	41.0	30.0	41.0
37	36.12617357715464	39.0	35.0	41.0	30.0	41.0
38	35.96819803412833	39.0	35.0	41.0	29.0	41.0
39	35.87629103881848	39.0	35.0	41.0	29.0	41.0
40	35.76542282041047	39.0	35.0	41.0	28.0	41.0
41	35.56425776702259	38.0	35.0	41.0	27.0	41.0
42	35.592886189003096	38.0	35.0	41.0	27.0	41.0
43	35.507389689984365	38.0	35.0	41.0	27.0	41.0
44	35.43325162991052	38.0	35.0	40.0	27.0	41.0
45	35.42833279280178	38.0	35.0	40.0	27.0	41.0
46	35.31972649103549	38.0	35.0	40.0	26.0	41.0
47	35.22852218674117	38.0	35.0	40.0	26.0	41.0
48	35.112981488873366	38.0	34.0	40.0	26.0	41.0
49	35.046682175764225	37.0	34.0	40.0	26.0	41.0
50	34.89102468150218	37.0	34.0	40.0	25.0	41.0
51	33.69947589229285	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	24.0
10	33.0
11	28.0
12	36.0
13	46.0
14	32.0
15	41.0
16	76.0
17	160.0
18	326.0
19	584.0
20	1071.0
21	1835.0
22	2740.0
23	3959.0
24	5930.0
25	9513.0
26	13059.0
27	14856.0
28	14902.0
29	14990.0
30	16418.0
31	19619.0
32	24780.0
33	35063.0
34	63783.0
35	75029.0
36	70744.0
37	103428.0
38	180960.0
39	287738.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.90860443069554	19.500403319695305	23.13267139008083	18.458320859528325
2	36.18099906862256	24.545537704154608	23.906255197418755	15.367208029804077
3	29.92590559824369	23.897523533912118	30.488682100921398	15.687888766922795
4	26.58344559757842	23.32799038685427	32.23106476399561	17.8574992515717
5	25.29677261085055	26.923564680836908	29.365519908192795	18.41414280011975
6	24.022677377507236	31.89292069986362	29.106688454246083	14.977713468383062
7	74.10739530319663	5.3503268136912485	15.469285334131659	5.072992548980474
8	75.75903103482686	5.961439310780694	13.554348368426306	4.725181285966138
9	70.08625636164055	6.452283537903735	15.399847819578886	8.061612280876826
10	39.710150350929716	24.074755513421813	21.813150716827995	14.401943418820476
11	29.979023217909056	22.902217842530685	29.067084123340987	18.051674816219272
12	26.21817100755081	19.673893157702157	33.113170674916006	20.994765159831022
13	25.00498952200379	20.828863553204936	35.36905831088048	18.797088613910788
14	19.838235538702058	24.605411968200112	35.076963376908495	20.479389116189335
15	18.7207905066028	22.757937497921034	38.68490752752553	19.836364467950638
16	23.7243455410305	24.04097229152114	32.364222632471815	19.87045953497655
17	23.383602767521538	23.659377806606127	30.960191930279745	21.996827495592587
18	24.833890496623756	22.47291105345441	31.999467784319595	20.693730665602235
19	23.82236885873	25.28804094734391	29.531941256694278	21.35764893723181
20	23.626530120081163	26.219418388051757	30.485875494794268	19.668175997072815
21	22.891303263147392	26.969094069121514	32.0093428799521	18.130259787778996
22	21.71783005688055	23.1278897648272	32.95101120979277	22.203268968499483
23	20.652671057446028	24.9356559558261	32.495717326946746	21.915955659781126
24	21.72832884276353	23.471647041213455	32.35403569171407	22.44598842430895
25	22.466362305824436	25.781899677344246	29.439323254498884	22.312414762332438
26	20.00569637095433	25.010082992382664	30.37849682333766	24.60572381332535
27	21.56939177726774	24.14471276984998	31.50259455144197	22.78330090144031
28	20.00226607457672	24.55801150916409	31.49978794531484	23.939934470944348
29	22.0373673618734	25.401552572930182	30.46123972990054	22.099840335295877
30	22.800868176828658	24.12194807570768	32.08636862588564	20.99081512157802
31	23.62268403020324	23.75667348568007	30.100954661876727	22.519687822239963
32	25.996760968632536	24.152716794731067	28.645365565645477	21.205156670990917
33	24.483480524232444	22.596089877923028	30.306148754282674	22.614280843561854
34	22.795151016199313	24.022157635631842	32.00414546119815	21.178545886970696
35	23.610314173568838	24.975468183481357	30.728075208728338	20.68614243422147
36	23.75054053155041	26.146966370621694	29.54846904833184	20.554024049496057
37	23.66457522536008	25.469950603732162	30.04835678408675	20.81711738682101
38	23.74024964241759	24.820793001363803	28.995047899411237	22.443909456807372
39	24.892517380168314	22.786627249442837	29.418429631108005	22.902425739280844
40	26.386775271928947	22.150255297209196	30.65583108804843	20.807138342813424
41	22.272602534677176	23.33994444998836	30.147523533912118	24.239929481422344
42	23.079241925290223	24.159681335861357	30.81528789541962	21.945788843428797
43	22.332268901972522	23.29503875195423	30.954578718025477	23.418113628047767
44	22.853570002993713	22.954088081695108	30.121432491767287	24.070909423543892
45	22.178945048731	22.80097212520374	29.27071899012075	25.749363835944518
46	23.147847852842364	23.832763696237897	28.88974819545621	24.129640255463528
47	20.61639307454346	23.9619715264611	32.11828077703489	23.30335462196055
48	21.715543192628814	24.59106709243921	30.339516182683035	23.353873532248944
49	22.922279878920932	22.43164354854805	31.473177161294615	23.172899411236404
50	21.493613411835145	22.726441140272094	31.370060373216248	24.409885074676513
51	20.702462329108872	22.943589295812128	29.899918504473938	26.454029870605062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	482.0
1	994.5
2	1507.0
3	10701.5
4	19896.0
5	12753.0
6	5610.0
7	5411.0
8	5212.0
9	5204.0
10	5196.0
11	5202.5
12	5209.0
13	5053.0
14	4897.0
15	4760.0
16	4623.0
17	4492.0
18	4361.0
19	4212.5
20	4064.0
21	4170.0
22	4276.0
23	4579.5
24	4883.0
25	5261.5
26	6560.5
27	7481.0
28	8611.5
29	9742.0
30	11773.5
31	13805.0
32	15003.0
33	16201.0
34	19656.5
35	23112.0
36	25073.5
37	27035.0
38	27832.0
39	28629.0
40	31968.0
41	35307.0
42	39879.0
43	44451.0
44	49739.0
45	55027.0
46	72686.5
47	90346.0
48	89120.5
49	87895.0
50	86191.5
51	84488.0
52	74959.5
53	65431.0
54	60534.5
55	55638.0
56	53471.5
57	51305.0
58	48742.0
59	46179.0
60	41572.5
61	36966.0
62	33771.5
63	30577.0
64	27247.0
65	23917.0
66	20272.5
67	16628.0
68	13991.0
69	11354.0
70	9905.0
71	8456.0
72	7380.0
73	6304.0
74	5160.0
75	3370.0
76	2724.0
77	2278.0
78	1832.0
79	1292.5
80	753.0
81	532.0
82	311.0
83	216.5
84	122.0
85	101.5
86	81.0
87	55.0
88	29.0
89	17.5
90	6.0
91	6.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	962016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.41598749336291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6031359886787	24.9293745916334
2	9.327781957096015	6.2339409043827345
3	3.579534617650098	3.5884105204636585
4	1.8920221081668045	2.528951484146723
5	1.3208920332015812	2.2069455830773377
6	1.00148729426841	2.0079412140021033
7	0.8043940680369714	1.8815735482081128
8	0.6427666547595586	1.7182945997276904
9	0.5682996970475006	1.709126601211909
>10	6.017937978272094	41.40770425633157
>50	0.20083174864997827	4.10247045744761
>100	0.03341981982007551	1.950774920626815
>500	0.0024986781173754473	0.5725724260084802
>1k	0.004685021470078963	3.798119423054066
>5k	0.0	0.0
>10k+	3.123347646719309E-4	1.363799469677873
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13067	1.358293417157303	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGC	4589	0.4770190932375345	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	4176	0.4340884143299072	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCG	4039	0.41984748694408414	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTT	3912	0.40664604330905096	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	3486	0.36236403552539664	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCT	2260	0.2349233276785417	No Hit
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	2190	0.2276469414230117	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	2041	0.21215863353624057	No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	2010	0.20893623390879154	No Hit
CGTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCT	1502	0.15613045936865916	No Hit
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT	1474	0.15321990486644713	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCC	1441	0.1497896084888401	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1225	0.12733675947177592	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCT	1024	0.10644313608089678	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTC	1022	0.10623523933073879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0789675015800153E-4	0.0	0.0	0.1625752586235572	0.0
2	2.0789675015800153E-4	0.0	0.0	0.7061213119116522	0.0
3	2.0789675015800153E-4	0.0	0.0	1.1266964374812893	0.0
4	2.0789675015800153E-4	0.0	0.0	1.6543383893822972	0.0
5	2.0789675015800153E-4	0.0	0.0	3.236120812959452	0.0
6	2.0789675015800153E-4	0.0	0.0	4.617074975883977	0.0
7	2.0789675015800153E-4	0.0	0.0	5.730050227854838	0.0
8	2.0789675015800153E-4	0.0	0.0	7.036265509097562	0.0
9	2.0789675015800153E-4	0.0	0.0	7.774818714033862	0.0
10	2.0789675015800153E-4	0.0	0.0	9.261384426038653	0.0
11	2.0789675015800153E-4	0.0	0.0	10.45689468782224	0.0
12	2.0789675015800153E-4	0.0	0.0	11.841279147124371	0.0
13	2.0789675015800153E-4	0.0	0.0	12.361332867644613	0.0
14	2.0789675015800153E-4	0.0	0.0	12.683572830389515	0.0
15	2.0789675015800153E-4	0.0	0.0	13.015168146891527	0.0
16	2.0789675015800153E-4	0.0	0.0	13.476283138741975	0.0
17	2.0789675015800153E-4	0.0	0.0	14.00839487077138	0.0
18	2.0789675015800153E-4	0.0	0.0	14.649028706383262	0.0
19	3.118451252370023E-4	0.0	0.0	15.112950304360842	0.0
20	4.1579350031600306E-4	0.0	0.0	15.556082227322623	0.0
21	4.1579350031600306E-4	0.0	0.0	16.03060655955826	0.0
22	4.1579350031600306E-4	0.0	0.0	16.541408874696472	0.0
23	4.1579350031600306E-4	0.0	0.0	17.03661893357283	0.0
24	4.1579350031600306E-4	0.0	0.0	17.442745235006488	0.0
25	5.197418753950039E-4	0.0	0.0	17.820701526793734	0.0
26	5.197418753950039E-4	0.0	0.0	18.19044589694974	0.0
27	5.197418753950039E-4	0.0	0.0	18.625989588530754	0.0
28	5.197418753950039E-4	0.0	0.0	19.013820975950505	0.0
29	5.197418753950039E-4	0.0	0.0	19.427743405515084	0.0
30	5.197418753950039E-4	0.0	0.0	19.97659082593221	0.0
31	5.197418753950039E-4	0.0	0.0	20.40516997638293	0.0
32	5.197418753950039E-4	5.197418753950039E-4	0.0	20.84747031234408	0.0
33	5.197418753950039E-4	5.197418753950039E-4	0.0	21.30369973056581	0.0
34	6.236902504740046E-4	5.197418753950039E-4	0.0	21.75649885240994	0.0
35	6.236902504740046E-4	5.197418753950039E-4	0.0	22.198175498120612	0.0
36	8.315870006320061E-4	5.197418753950039E-4	0.0	22.59983201942587	0.0
37	8.315870006320061E-4	5.197418753950039E-4	0.0	23.047225825765892	0.0
38	9.355353757110069E-4	5.197418753950039E-4	0.0	23.54285167814257	0.0
39	0.0011434321258690084	5.197418753950039E-4	0.0	24.265292884941623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	7.0327136E-4	45.0	39
ATAACGC	25	3.89033E-5	45.0	11
ATACGCG	25	3.89033E-5	45.0	1
CAATACG	20	7.0327136E-4	45.0	15
TCGCGAC	20	7.0327136E-4	45.0	24
CGTTTTT	5805	0.0	43.294575	1
TACGCGG	75	0.0	42.0	2
TCGTAAG	60	3.6379788E-12	41.250004	1
CGTTTCT	435	0.0	40.862072	1
CGTTCTG	430	0.0	40.813953	1
TTAAGCG	45	1.927765E-8	40.0	1
TCGATAG	40	3.457444E-7	39.375	1
TACGATC	40	3.457444E-7	39.375	11
TATTGCG	75	0.0	39.0	1
AATAGCG	35	6.2472245E-6	38.57143	1
CGTAAGG	135	0.0	38.333336	2
CCGATGA	285	0.0	37.894733	18
TAGCCGT	90	0.0	37.5	44
TACGTAG	85	0.0	37.058823	1
TACGGGA	360	0.0	36.875	4
>>END_MODULE