##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545474
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.038857947399876	33.0	31.0	34.0	30.0	34.0
2	32.05458738638322	33.0	31.0	34.0	30.0	34.0
3	32.053524090974086	33.0	31.0	34.0	30.0	34.0
4	35.73526144234189	37.0	35.0	37.0	33.0	37.0
5	35.779925349329204	37.0	35.0	37.0	35.0	37.0
6	35.70881471894169	37.0	35.0	37.0	35.0	37.0
7	35.95311600552914	37.0	35.0	37.0	35.0	37.0
8	35.765565361502105	37.0	35.0	37.0	35.0	37.0
9	37.554935707293104	39.0	37.0	39.0	35.0	39.0
10	37.26183832776631	39.0	37.0	39.0	34.0	39.0
11	37.22030014262825	39.0	37.0	39.0	34.0	39.0
12	36.92966300868602	39.0	35.0	39.0	33.0	39.0
13	36.74168521322739	39.0	35.0	39.0	33.0	39.0
14	37.57011699916036	40.0	36.0	41.0	32.0	41.0
15	37.703098589483645	40.0	36.0	41.0	33.0	41.0
16	37.68168052006145	40.0	36.0	41.0	33.0	41.0
17	37.68412243296656	40.0	36.0	41.0	33.0	41.0
18	37.66814916934629	39.0	36.0	41.0	33.0	41.0
19	37.549256243193994	39.0	35.0	41.0	33.0	41.0
20	37.572989730032965	39.0	35.0	41.0	33.0	41.0
21	37.55900189559906	39.0	35.0	41.0	33.0	41.0
22	37.691792826055874	39.0	35.0	41.0	33.0	41.0
23	37.73262886957032	40.0	35.0	41.0	34.0	41.0
24	37.6833597935007	40.0	35.0	41.0	33.0	41.0
25	37.43588878663328	39.0	35.0	41.0	33.0	41.0
26	37.481504159684974	39.0	35.0	41.0	33.0	41.0
27	37.45353765715689	39.0	35.0	41.0	33.0	41.0
28	37.22471098530819	39.0	35.0	41.0	33.0	41.0
29	37.08434865823119	39.0	35.0	41.0	32.0	41.0
30	36.941256595181436	39.0	35.0	41.0	32.0	41.0
31	36.88567374430312	39.0	35.0	41.0	31.0	41.0
32	36.83081503426378	39.0	35.0	41.0	32.0	41.0
33	36.744592776190984	39.0	35.0	41.0	31.0	41.0
34	36.54223666022578	39.0	35.0	41.0	31.0	41.0
35	36.51230122792287	39.0	35.0	41.0	31.0	41.0
36	36.361703765899016	39.0	35.0	41.0	30.0	41.0
37	36.362585567781416	39.0	35.0	41.0	30.0	41.0
38	36.167118872760206	39.0	35.0	41.0	30.0	41.0
39	36.107775989323045	38.0	35.0	41.0	30.0	41.0
40	36.01667907177978	38.0	35.0	41.0	30.0	41.0
41	35.797493556063166	38.0	35.0	41.0	29.0	41.0
42	35.87798501853434	38.0	35.0	40.0	29.0	41.0
43	35.772748105317575	38.0	35.0	40.0	29.0	41.0
44	35.70988168088672	38.0	35.0	40.0	29.0	41.0
45	35.696546489841865	38.0	35.0	40.0	29.0	41.0
46	35.547663133348244	37.0	35.0	40.0	29.0	41.0
47	35.47047705298511	37.0	35.0	40.0	29.0	41.0
48	35.36801937397566	37.0	35.0	40.0	29.0	41.0
49	35.30912197464957	37.0	35.0	40.0	28.0	41.0
50	35.134354708015415	37.0	34.0	40.0	28.0	41.0
51	33.95192254809579	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	14.0
11	4.0
12	14.0
13	17.0
14	21.0
15	21.0
16	58.0
17	99.0
18	200.0
19	369.0
20	678.0
21	1209.0
22	1990.0
23	2582.0
24	3422.0
25	4366.0
26	5273.0
27	6347.0
28	6953.0
29	7869.0
30	9410.0
31	12019.0
32	15886.0
33	23151.0
34	44983.0
35	49119.0
36	40345.0
37	58256.0
38	98497.0
39	152183.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78215276988454	19.437590059287885	23.156007435734793	12.624249735092782
2	31.444761803495673	27.31789232850695	23.20055584684146	18.036790021155912
3	30.85683277296443	26.98130433347877	25.720565966480528	16.44129692707627
4	27.350524497959572	23.334751060545507	30.045428379721123	19.269296061773797
5	27.869705980486696	27.035935718292713	25.05710629654209	20.0372520046785
6	24.653054041072537	35.00533480972512	25.368945174288783	14.972665974913562
7	76.05422073279387	5.6666312234863625	13.482035807389536	4.797112236330237
8	76.662682364329	8.640008506363273	9.521443735173445	5.175865394134275
9	71.4732507873886	6.780524827947803	11.196134004553837	10.550090380109776
10	44.986012165566095	22.961681033376475	17.613488452245203	14.438818348812227
11	36.65124277234112	22.32223717354081	22.805119950721757	18.221400103396313
12	31.223853015909096	19.39524156971735	28.042583147867727	21.338322266505827
13	29.24300699941702	21.57133062254113	30.795968277131447	18.3896941009104
14	22.014248158482346	25.85751108210474	31.423679222107744	20.704561537305167
15	20.132215284321525	21.346388645471645	37.33431840931007	21.18707766089676
16	25.95210770815841	23.37141641948104	27.63229778137913	23.044178090981422
17	25.677850823320636	22.730139291698595	27.70526184566084	23.88674803931993
18	28.77185713709544	21.073781701785972	28.305657098230164	21.848704062888423
19	26.520420771659143	24.18795396297532	25.739815279921686	23.551809985443853
20	26.81576023788485	25.891243212325428	27.307626028005	19.985370521784724
21	25.329163259843735	27.554750547230483	28.952617356647615	18.163468836278174
22	23.828450118612434	22.18290880958579	30.732170552583625	23.256470519218148
23	23.065993979548065	25.440625951007746	29.370969102102023	22.122410967342166
24	24.277600765572693	23.194139409027745	27.62588134356541	24.90237848183415
25	23.714420852322935	27.4849030384583	25.102021361238116	23.698654747980655
26	21.930651140109337	23.69132167619355	26.87204156385089	27.505985619846225
27	23.661806062250445	23.493512064736358	27.450987581442927	25.393694291570267
28	20.730777268944074	24.96470959202455	29.106061883792812	25.198451255238563
29	24.027359690837695	26.054220732793866	26.77249511434092	23.14592446202752
30	26.628950234108316	24.04165918082255	28.25817545840865	21.071215126660483
31	26.55965270572016	23.4135815822569	26.644166358066563	23.382599353956376
32	28.015634109050108	23.91259711736948	25.395344232722366	22.676424540858044
33	26.758195624356063	21.457301356251627	27.289293348537235	24.495209670855072
34	22.278788723202204	23.70213795707953	30.622724456161066	23.3963488635572
35	22.81043642776741	25.674734267811118	27.495169338960245	24.019659965461233
36	24.007927050601864	27.117882795513626	27.89537173174157	20.978818422142943
37	23.45336349670195	26.058070595482096	26.517487542944302	23.971078364871655
38	22.81355298327693	25.392411004007524	26.737663023352166	25.05637298936338
39	23.260503708701055	22.264672560012023	26.429674008293702	28.045149722993212
40	25.478941251095378	20.753509791484102	27.698662081052444	26.068886876368076
41	20.86423917546941	22.336903317115024	26.89385745241753	29.905000054998037
42	22.454965772887434	21.94366734253145	28.97864976149184	26.62271712308928
43	22.00801504746331	22.334886722373568	29.263906254010273	26.393191976152853
44	23.348133916556975	21.093031015227123	28.318856627446955	27.239978440768947
45	22.099495118007457	21.173328151295937	27.12319927255928	29.603977458137326
46	23.29093595661755	23.965028580647292	26.503004726164768	26.241030736570398
47	19.281028976633166	23.538977109816418	31.99125897842977	25.18873493512065
48	21.67546024191804	24.482743448816993	28.485684010603624	25.356112298661348
49	24.361564437535062	20.585582447559368	30.403648936521265	24.649204178384306
50	21.367104573270222	22.465232073389384	29.322754155101798	26.844909198238597
51	21.52348232913026	22.142943568346062	26.914390053421428	29.41918404910225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	598.5
2	952.0
3	2356.0
4	3760.0
5	2428.0
6	1096.0
7	1108.0
8	1120.0
9	1156.5
10	1193.0
11	1183.5
12	1174.0
13	1155.0
14	1136.0
15	1138.0
16	1140.0
17	1169.5
18	1199.0
19	1246.5
20	1294.0
21	1344.5
22	1395.0
23	1363.0
24	1331.0
25	1658.0
26	2629.5
27	3274.0
28	3491.0
29	3708.0
30	4489.5
31	5271.0
32	6187.5
33	7104.0
34	8432.5
35	9761.0
36	10808.5
37	11856.0
38	12931.5
39	14007.0
40	15446.5
41	16886.0
42	19291.5
43	21697.0
44	24454.0
45	27211.0
46	42214.5
47	57218.0
48	52166.0
49	47114.0
50	48714.5
51	50315.0
52	45552.0
53	40789.0
54	39225.5
55	37662.0
56	36837.0
57	36012.0
58	35228.0
59	34444.0
60	30735.5
61	27027.0
62	24570.5
63	22114.0
64	19264.5
65	16415.0
66	14641.5
67	12868.0
68	10664.0
69	8460.0
70	7288.5
71	6117.0
72	5114.0
73	4111.0
74	3159.0
75	1800.0
76	1393.0
77	1066.0
78	739.0
79	565.5
80	392.0
81	270.5
82	149.0
83	104.5
84	60.0
85	55.0
86	50.0
87	29.5
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	4.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	545474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.16506693128535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70605996465795	24.77631478900498
2	9.066654663848645	6.013924175387817
3	3.3563453486786985	3.339402544003119
4	1.8206601893651535	2.415292681576879
5	1.1895289598843068	1.972540378563264
6	0.8986055961423804	1.7881388844533774
7	0.7522019225545166	1.7462778975153446
8	0.6469347163298694	1.716450653380178
9	0.5798654312508931	1.7308148254717062
>10	6.858806708205749	45.054788882670024
>50	0.07715523826954276	1.7195898250693609
>100	0.03774500864984803	2.0990004480720224
>500	0.0049956629095387105	1.197324935472762
>1k	0.003330441939692473	2.2598955885399183
>5k	0.0011101473132308244	2.170243490819248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	5954	1.0915277355107669	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	5835	1.0697118469441258	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	4917	0.9014178494300369	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2217	0.4064355038003645	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1805	0.33090486439316996	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1243	0.22787520578432702	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1055	0.1934097683849276	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	1039	0.1904765396700851	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT	974	0.17856029801603743	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	948	0.17379380135441835	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA	825	0.1512446056090666	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	796	0.14592812856341456	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC	675	0.12374558640741813	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT	625	0.1145792466735353	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC	586	0.10742950168110671	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	574	0.10522958014497483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.33307178710626E-4	0.0	0.0	0.1534445271451985	0.0
2	7.33307178710626E-4	0.0	0.0	0.8434865823118975	0.0
3	9.166339733882824E-4	0.0	0.0	1.17732467541991	0.0
4	9.166339733882824E-4	0.0	0.0	1.7130055694680224	0.0
5	9.166339733882824E-4	0.0	0.0	3.473126125168202	0.0
6	9.166339733882824E-4	0.0	0.0	4.283980538027477	0.0
7	9.166339733882824E-4	0.0	0.0	5.115184225095971	0.0
8	9.166339733882824E-4	0.0	0.0	6.14401419682698	0.0
9	9.166339733882824E-4	0.0	0.0	6.630930163490835	0.0
10	9.166339733882824E-4	0.0	0.0	8.548345109024444	0.0
11	9.166339733882824E-4	0.0	0.0	9.710636987280788	0.0
12	9.166339733882824E-4	0.0	0.0	11.696982807613194	0.0
13	0.001099960768065939	0.0	0.0	12.22148076718597	0.0
14	0.001099960768065939	0.0	0.0	12.537902814799606	0.0
15	0.001099960768065939	0.0	0.0	12.992736592394872	0.0
16	0.001099960768065939	0.0	0.0	13.519617800298455	0.0
17	0.001099960768065939	0.0	0.0	14.105163582498891	0.0
18	0.001099960768065939	0.0	0.0	14.81793816020562	0.0
19	0.001099960768065939	0.0	0.0	15.415400184060102	0.0
20	0.001099960768065939	0.0	0.0	15.875183785111664	0.0
21	0.001099960768065939	0.0	0.0	16.35989983023939	0.0
22	0.001099960768065939	0.0	0.0	16.884397789812162	0.0
23	0.001099960768065939	0.0	0.0	17.330798534852256	0.0
24	0.0012832875627435956	0.0	0.0	17.73100092763358	0.0
25	0.001466614357421252	0.0	0.0	18.093804654300662	0.0
26	0.001466614357421252	0.0	0.0	18.471641178131314	0.0
27	0.001466614357421252	0.0	0.0	18.979273072593745	0.0
28	0.001466614357421252	0.0	0.0	19.36040947872859	0.0
29	0.001466614357421252	0.0	0.0	19.769044904065087	0.0
30	0.001466614357421252	0.0	0.0	20.344324385763574	0.0
31	0.001466614357421252	0.0	0.0	20.743060164187476	0.0
32	0.001466614357421252	0.0	0.0	21.150045648371876	0.0
33	0.0016499411520989084	0.0	0.0	21.60267950443101	0.0
34	0.0016499411520989084	0.0	0.0	22.100411751980847	0.0
35	0.0016499411520989084	0.0	0.0	22.572111594686458	0.0
36	0.0016499411520989084	0.0	0.0	22.981113673612306	0.0
37	0.0016499411520989084	0.0	0.0	23.401482013808174	0.0
38	0.0016499411520989084	0.0	0.0	23.920113515951265	0.0
39	0.0016499411520989084	0.0	0.0	24.875246116221856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	20	7.030022E-4	45.000004	1
TAGACGG	20	7.030022E-4	45.000004	2
GACGTAG	20	7.030022E-4	45.000004	9
GGACGTA	30	2.163395E-6	45.000004	8
ATCGGAT	30	2.163395E-6	45.000004	25
CGTCAAG	20	7.030022E-4	45.000004	10
TATTACG	25	3.8881015E-5	45.0	1
AACGAAG	45	3.8380676E-10	45.0	1
CGACAGG	55	6.002665E-11	40.909092	2
CGCGAAT	50	1.0786607E-9	40.5	27
CCGATGA	325	0.0	40.153843	18
TACGAAT	90	0.0	40.0	12
GTCATAC	40	3.4537698E-7	39.375004	39
CACGCCA	40	3.4537698E-7	39.375004	27
CCATAGG	40	3.4537698E-7	39.375004	2
CGTCATA	40	3.4537698E-7	39.375004	38
TAGGGTA	75	0.0	39.000004	5
AGGGTAC	110	0.0	38.863636	6
AACGGGA	180	0.0	38.75	4
ATTACGG	35	6.242253E-6	38.571426	2
>>END_MODULE