FastQCFastQC Report
Sat 14 Jan 2017
SRR2935606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840964
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC232912.7695596957777027No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132131.5711730823198138No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG123301.466174533035897No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC107001.2723493514585642No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT64310.7647176335728997No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT39560.47041252657664295No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC38630.4593537892228443No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT27410.32593547405120793No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT27250.32403289558173715No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC23790.2828896361794322No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC21680.25779938261328667No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT17430.20726214201796986No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT16300.19382518157733267No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG15050.17896128728459243No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA13610.16183808105935568No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG12050.14328794098201586No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC10720.12747275745454026No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC10050.11950571011363151No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT9130.1085658839141747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT253.88991E-545.038
CGTTTTT58700.043.7350921
TACGGCT13150.042.7756657
CGATGAA11150.042.1748919
CCGATGA11100.041.75675618
ATCGAAT1300.041.5384643
CGTAAGG1200.041.2499962
ACGGCTG13700.041.2226268
CGGGTAT556.002665E-1140.9090926
GCGAGAC1950.040.38461721
GATGAAT11900.040.2731120
TTTAGCG403.456753E-739.3751
TCGGGTA403.456753E-739.3755
AAACACG2000.039.37540
CGGCTGT14000.039.3759
CGATCGA1200.039.37499641
GAATCTG28450.039.30581
GCGTAAG750.039.01
CGTCTCG356.2462877E-638.5714342
CCGTAGG356.2462877E-638.571432
Produced by FastQC (version 0.11.5)