##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935605.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324886
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.124040432644065	33.0	31.0	34.0	30.0	34.0
2	32.142496137106555	33.0	31.0	34.0	30.0	34.0
3	32.139744402652006	33.0	31.0	34.0	30.0	34.0
4	35.80088708039128	37.0	35.0	37.0	35.0	37.0
5	35.885830722161	37.0	35.0	37.0	35.0	37.0
6	35.83211034024242	37.0	35.0	37.0	35.0	37.0
7	36.082890613938424	37.0	35.0	37.0	35.0	37.0
8	35.89207906773453	37.0	35.0	37.0	35.0	37.0
9	37.68624379012946	39.0	38.0	39.0	35.0	39.0
10	37.39434755575802	39.0	37.0	39.0	34.0	39.0
11	37.361797676723526	39.0	37.0	39.0	35.0	39.0
12	37.155417592632496	39.0	37.0	39.0	34.0	39.0
13	37.02397764138806	39.0	37.0	39.0	33.0	39.0
14	38.103427048256926	40.0	37.0	41.0	33.0	41.0
15	38.23450379517738	40.0	37.0	41.0	34.0	41.0
16	38.04596381499972	40.0	36.0	41.0	34.0	41.0
17	38.07642373016997	40.0	36.0	41.0	34.0	41.0
18	38.02041023620593	40.0	36.0	41.0	34.0	41.0
19	37.86541740795233	40.0	36.0	41.0	34.0	41.0
20	38.03624040432644	40.0	36.0	41.0	34.0	41.0
21	37.93828296694841	40.0	36.0	41.0	34.0	41.0
22	38.0260737612578	40.0	36.0	41.0	34.0	41.0
23	38.067571394273685	40.0	36.0	41.0	34.0	41.0
24	37.989965711049415	40.0	35.0	41.0	34.0	41.0
25	37.729249644490686	40.0	35.0	41.0	34.0	41.0
26	37.8088775755188	40.0	35.0	41.0	34.0	41.0
27	37.833098379123754	40.0	35.0	41.0	34.0	41.0
28	37.71585109853918	40.0	35.0	41.0	33.0	41.0
29	37.70745430704924	40.0	35.0	41.0	34.0	41.0
30	37.53581563994755	40.0	35.0	41.0	33.0	41.0
31	37.498590274742526	40.0	35.0	41.0	33.0	41.0
32	37.415487278614656	40.0	35.0	41.0	33.0	41.0
33	37.36855697075282	40.0	35.0	41.0	33.0	41.0
34	37.25647150077258	40.0	35.0	41.0	33.0	41.0
35	37.131532291326806	40.0	35.0	41.0	32.0	41.0
36	37.038234950105576	39.0	35.0	41.0	32.0	41.0
37	37.034473630750476	39.0	35.0	41.0	32.0	41.0
38	36.904104824461506	39.0	35.0	41.0	32.0	41.0
39	36.865552840073136	39.0	35.0	41.0	32.0	41.0
40	36.77586907407522	39.0	35.0	41.0	31.0	41.0
41	36.65299212646897	39.0	35.0	41.0	31.0	41.0
42	36.6541956255425	39.0	35.0	41.0	31.0	41.0
43	36.5787691682621	39.0	35.0	41.0	31.0	41.0
44	36.452549509674164	39.0	35.0	41.0	31.0	41.0
45	36.41904237178579	39.0	35.0	41.0	31.0	41.0
46	36.25782274397789	38.0	35.0	41.0	31.0	41.0
47	36.18366134582592	38.0	35.0	41.0	31.0	41.0
48	36.08976071606656	38.0	35.0	40.0	30.0	41.0
49	36.084463473341415	38.0	35.0	40.0	31.0	41.0
50	35.946002597834315	38.0	35.0	40.0	30.0	41.0
51	34.78623578732232	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	5.0
10	4.0
11	8.0
12	10.0
13	9.0
14	11.0
15	15.0
16	28.0
17	52.0
18	77.0
19	171.0
20	296.0
21	460.0
22	679.0
23	930.0
24	1293.0
25	1802.0
26	2364.0
27	2789.0
28	3221.0
29	3773.0
30	4654.0
31	5788.0
32	7648.0
33	10991.0
34	23192.0
35	31789.0
36	22385.0
37	33681.0
38	60689.0
39	105990.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15702738806843	20.426549620482263	22.931120454559444	12.485302536889863
2	31.896419051605797	24.589240533602556	25.2146906915041	18.299649723287555
3	31.694809871770406	24.10691750337041	26.883583780156734	17.31468884470245
4	29.14499239733322	24.926589634517953	26.476056216642146	19.452361751506682
5	25.754264572803997	29.89725626835259	24.80439292551849	19.54408623332492
6	25.655460684670935	34.3234242164944	24.997075897391703	15.024039201442967
7	77.20892867036437	6.8291646916149045	10.452281723435297	5.509624914585424
8	77.58875420916875	6.6626447430791105	10.05183356623554	5.696767481516594
9	71.11417543384448	8.308452811139908	12.407429067426728	8.169942687588877
10	42.77685095695105	23.781572613162773	18.857999421335485	14.58357700855069
11	33.018658852643696	25.306415173322332	23.361117438116754	18.313808535917214
12	30.123797270427165	22.62947618549276	28.540164857827055	18.706561686253025
13	24.871185585097543	26.404646552944726	30.359264480463917	18.364903381493818
14	20.789753944460518	28.859661542818095	30.00129276115314	20.349291751568245
15	19.63981211871241	24.844714761485566	36.406924274976454	19.10854884482557
16	22.607930166273707	24.353465523291245	32.699469967927214	20.339134342507833
17	22.37954236255179	24.064441065481432	28.997556065820014	24.55846050614677
18	25.30733857414601	23.49593395837309	30.691996577260948	20.504730890219953
19	24.604938347605007	27.192307455538252	26.611796137722155	21.59095805913459
20	25.829675640070672	26.107311487721844	28.435820564751946	19.627192307455537
21	24.586778131406092	27.429621467222347	28.318548660145403	19.665051741226154
22	22.670721422283506	25.773963790375703	28.541088258650728	23.014226528690063
23	22.673799425029088	25.797356611242094	28.626964535252363	22.90187942847645
24	24.27436085272988	24.030583035280067	29.899410870274494	21.795645241715555
25	21.729775982960177	26.27690943900322	27.64600506023651	24.34730951780009
26	20.67340544067765	26.565010495989362	29.34044557167745	23.421138491655537
27	20.672789840128537	26.160253134945798	29.089896148187368	24.0770608767383
28	19.0155931619091	26.876504373841904	30.561489260848422	23.546413203400576
29	21.300086799677427	25.27717414723934	30.633822325369515	22.78891672771372
30	22.11021712231367	26.406185554317513	29.03910910288532	22.444488220483493
31	25.12573641215688	26.650271172041883	25.514795959197993	22.70919645660324
32	25.271941542571856	27.579520200932016	25.93648233534224	21.212055921153883
33	24.407638371613427	27.518267946295012	24.655109792357933	23.41898388973363
34	22.465418639153427	26.299071058771382	28.860584943641772	22.37492535843342
35	21.84520108591937	27.509957338881946	27.86669785709449	22.778143718104197
36	21.771636820300046	29.163768214081248	27.071649747911575	21.992945217707135
37	22.300129891715866	28.458289984794664	27.322814771950775	21.918765351538696
38	21.9720147990372	30.85851652579674	23.779418011240868	23.39005066392519
39	23.802810832107262	27.730342335465362	26.707829823384206	21.759017009043173
40	24.033353237751086	25.3913680491003	28.039681611396	22.535597101752614
41	19.821722080976095	26.661351981925968	27.710335317619105	25.806590619478833
42	21.477071957548187	25.549577390223032	27.860234051328774	25.11311660090001
43	22.084054098976257	25.501868347666566	26.998393282566806	25.41568427079037
44	21.833812475760727	25.838294047758293	27.09504256877797	25.232850907703007
45	22.15084675855531	26.282142043670703	25.969109164445374	25.597902033328612
46	22.64055699537684	27.15506362231675	27.31265736289037	22.89172201941604
47	20.80268155599195	26.197496968167293	29.756899343154213	23.24292213268654
48	21.914148347420326	25.13281581847171	29.162537012983016	23.79049882112495
49	21.763326212886984	24.75299027966733	29.617773619054066	23.86590988839162
50	20.13260035827952	25.310724377166142	29.803684984887006	24.75299027966733
51	20.238175852452862	25.00353970315741	27.580443601755693	27.177840842634033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	564.0
2	836.0
3	1267.5
4	1699.0
5	1200.5
6	702.0
7	703.0
8	704.0
9	726.0
10	748.0
11	803.0
12	858.0
13	840.0
14	822.0
15	799.0
16	776.0
17	809.5
18	843.0
19	778.5
20	714.0
21	1056.5
22	1399.0
23	1615.0
24	1831.0
25	2071.0
26	2768.5
27	3226.0
28	3603.5
29	3981.0
30	4545.0
31	5109.0
32	5482.0
33	5855.0
34	7158.0
35	8461.0
36	8761.0
37	9061.0
38	9945.5
39	10830.0
40	12591.0
41	14352.0
42	16124.5
43	17897.0
44	20919.5
45	23942.0
46	25945.5
47	27949.0
48	29746.0
49	31543.0
50	30885.5
51	30228.0
52	26643.5
53	23059.0
54	20740.5
55	18422.0
56	17599.0
57	16776.0
58	15379.5
59	13983.0
60	13500.0
61	13017.0
62	11882.5
63	10748.0
64	9423.5
65	8099.0
66	6723.0
67	5347.0
68	4213.0
69	3079.0
70	2552.5
71	2026.0
72	1753.5
73	1481.0
74	1190.5
75	735.5
76	571.0
77	396.0
78	221.0
79	170.0
80	119.0
81	75.0
82	31.0
83	25.0
84	19.0
85	12.5
86	6.0
87	8.5
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	324886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.776333852489795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75485058206985	22.69903904754283
2	10.25523062767532	6.312368030632283
3	3.581429771572589	3.3066983495749276
4	1.7392087050446055	2.141058709824369
5	1.1161339360723288	1.7175255320327747
6	0.7590910909309116	1.4017224503364256
7	0.5810697283674041	1.2518237166267552
8	0.48805856702804334	1.2016522718738265
9	0.4240508861063328	1.174565847712736
>10	6.941833019962395	48.91654303355639
>50	0.3060367244069288	5.744784324347617
>100	0.046005520662479496	2.4448575808129624
>500	0.00500060007200864	0.9898856829780293
>1k	0.0020002400288034565	0.6974754221480766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1241	0.3819801407262855	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1025	0.31549528142179106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	858	0.26409263557063095	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	710	0.21853819493606988	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	584	0.17975536034178144	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	554	0.17052135210504607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	510	0.1569781400245009	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	493	0.15174553535701754	No Hit
AAGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTACTCTTCCAAA	380	0.11696410433198105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.078002745578449E-4	0.0	0.0	0.2296190048201523	0.0
2	3.078002745578449E-4	0.0	0.0	1.1736424468890627	0.0
3	3.078002745578449E-4	0.0	0.0	1.5993302266025622	0.0
4	3.078002745578449E-4	0.0	0.0	2.1693763350836908	0.0
5	3.078002745578449E-4	0.0	0.0	3.569867584321885	0.0
6	3.078002745578449E-4	0.0	0.0	4.314436448477312	0.0
7	3.078002745578449E-4	0.0	0.0	5.049155703846888	0.0
8	3.078002745578449E-4	0.0	0.0	6.174165707355811	0.0
9	3.078002745578449E-4	0.0	0.0	6.61001089612972	0.0
10	3.078002745578449E-4	0.0	0.0	7.696238065044354	0.0
11	3.078002745578449E-4	0.0	0.0	9.039170662940231	0.0
12	3.078002745578449E-4	0.0	0.0	10.218045714496776	0.0
13	3.078002745578449E-4	0.0	0.0	10.720991363124297	0.0
14	3.078002745578449E-4	0.0	0.0	10.912135333624718	0.0
15	3.078002745578449E-4	0.0	0.0	11.27595525815209	0.0
16	3.078002745578449E-4	0.0	0.0	11.959271867670505	0.0
17	3.078002745578449E-4	0.0	0.0	12.70137832962947	0.0
18	3.078002745578449E-4	0.0	0.0	13.59399912584722	0.0
19	6.156005491156898E-4	0.0	0.0	14.139421212363722	0.0
20	6.156005491156898E-4	0.0	0.0	14.695924108764306	0.0
21	6.156005491156898E-4	0.0	0.0	15.369698909771428	0.0
22	6.156005491156898E-4	0.0	0.0	15.991147664103716	0.0
23	6.156005491156898E-4	0.0	0.0	16.578738388234644	0.0
24	6.156005491156898E-4	0.0	0.0	17.074296830272772	0.0
25	6.156005491156898E-4	0.0	0.0	17.54307664842437	0.0
26	6.156005491156898E-4	0.0	0.0	18.008162863281274	0.0
27	6.156005491156898E-4	0.0	0.0	18.443700251780626	0.0
28	6.156005491156898E-4	0.0	0.0	18.902014860597255	0.0
29	6.156005491156898E-4	0.0	0.0	19.34955645980436	0.0
30	6.156005491156898E-4	0.0	0.0	19.87004672408168	0.0
31	6.156005491156898E-4	0.0	0.0	20.328053532623752	0.0
32	6.156005491156898E-4	0.0	0.0	20.803912757090178	0.0
33	6.156005491156898E-4	0.0	0.0	21.305627204619466	0.0
34	6.156005491156898E-4	0.0	0.0	21.768866617829023	0.0
35	6.156005491156898E-4	0.0	0.0	22.255498851904974	0.0
36	6.156005491156898E-4	0.0	0.0	22.72212406813467	0.0
37	6.156005491156898E-4	0.0	0.0	23.173051470361912	0.0
38	6.156005491156898E-4	0.0	0.0	23.670764514321945	0.0
39	6.156005491156898E-4	0.0	0.0	24.181097369538854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	20	7.0258067E-4	45.000004	1
TACGGGT	20	7.0258067E-4	45.000004	4
CGCGAGG	35	1.2083365E-7	45.000004	2
TCTAGCG	20	7.0258067E-4	45.000004	1
CGTTAGG	40	6.7884685E-9	45.000004	2
TTATACG	20	7.0258067E-4	45.000004	1
ATTCCGG	20	7.0258067E-4	45.000004	11
CTACGAA	35	1.2083365E-7	45.000004	11
TCGTCCC	20	7.0258067E-4	45.000004	38
AGTTACT	20	7.0258067E-4	45.000004	17
GGCGCTA	20	7.0258067E-4	45.000004	34
TAGGCAG	35	1.2083365E-7	45.000004	1
GATTCCG	20	7.0258067E-4	45.000004	10
CCACGAA	20	7.0258067E-4	45.000004	31
AATACGG	20	7.0258067E-4	45.000004	2
GTACGAG	35	1.2083365E-7	45.000004	1
CGGGTAT	55	1.8189894E-12	45.0	6
CATACGA	45	3.8380676E-10	45.0	18
CTCGAGG	45	3.8380676E-10	45.0	2
GCGAGAC	25	3.8846083E-5	44.999996	21
>>END_MODULE