SRR2935604.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935604.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	7554	1.7149979453625355	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	6801	1.544043027059916	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	5546	1.2591181632222164	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2471	0.560995488878849	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	1379	0.3130768025754483	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT	1336	0.30331443672284186	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1248	0.28333564148960083	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	1070	0.24292398749509045	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	1061	0.2408807016189635	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1025	0.2327075581144558	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.23089130400234295	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	921	0.20909625465698906	Illumina PCR Primer Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	557	0.12645669255585548	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	545	0.12373231138768626	TruSeq Adapter, Index 13 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	25	3.8868748E-5	45.0	1
TCGCAAA	20	7.028543E-4	45.0	14
CGTGAGA	20	7.028543E-4	45.0	42
AGCCACG	20	7.028543E-4	45.0	42
TCCCTCG	20	7.028543E-4	45.0	11
CGTTACT	20	7.028543E-4	45.0	2
CAGGCGA	20	7.028543E-4	45.0	5
GATATCG	20	7.028543E-4	45.0	1
GATGCGA	20	7.028543E-4	45.0	11
CTCGCAC	20	7.028543E-4	45.0	27
ACGTCGG	20	7.028543E-4	45.0	10
CGTCGGC	20	7.028543E-4	45.0	11
TTACGGG	50	2.1827873E-11	45.0	3
TATGTCG	20	7.028543E-4	45.0	1
CCGTTAG	20	7.028543E-4	45.0	1
TACGGGT	30	2.162442E-6	44.999996	4
CGACAAT	30	2.162442E-6	44.999996	20
GTAGACG	30	2.162442E-6	44.999996	1
TCATCGA	30	2.162442E-6	44.999996	16
GTACGAG	30	2.162442E-6	44.999996	1