##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587581
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27093796429769	33.0	31.0	34.0	30.0	34.0
2	32.2885457494371	34.0	31.0	34.0	30.0	34.0
3	32.30896846562431	34.0	31.0	34.0	30.0	34.0
4	35.9410821656929	37.0	35.0	37.0	35.0	37.0
5	35.98616020599713	37.0	35.0	37.0	35.0	37.0
6	35.929497379935704	37.0	35.0	37.0	35.0	37.0
7	36.164785791235595	37.0	35.0	37.0	35.0	37.0
8	35.96897278843257	37.0	35.0	37.0	35.0	37.0
9	37.81065248876325	39.0	38.0	39.0	35.0	39.0
10	37.48558751899738	39.0	37.0	39.0	35.0	39.0
11	37.47819279384459	39.0	37.0	39.0	35.0	39.0
12	37.344907340434766	39.0	37.0	39.0	35.0	39.0
13	37.298903470330046	39.0	37.0	39.0	34.0	39.0
14	38.473948272663684	40.0	38.0	41.0	34.0	41.0
15	38.58910856545736	40.0	38.0	41.0	35.0	41.0
16	38.38325099007626	40.0	37.0	41.0	34.0	41.0
17	38.42887363614548	40.0	38.0	41.0	34.0	41.0
18	38.34424700594471	40.0	37.0	41.0	34.0	41.0
19	38.19268662533336	40.0	37.0	41.0	34.0	41.0
20	38.33243927220247	40.0	37.0	41.0	34.0	41.0
21	38.266880651348494	40.0	37.0	41.0	34.0	41.0
22	38.352131876285995	40.0	37.0	41.0	34.0	41.0
23	38.368718525616046	40.0	37.0	41.0	34.0	41.0
24	38.30061727659676	40.0	37.0	41.0	34.0	41.0
25	38.0336208965232	40.0	36.0	41.0	34.0	41.0
26	38.11005631563989	40.0	37.0	41.0	34.0	41.0
27	38.11692005017181	40.0	37.0	41.0	34.0	41.0
28	38.00869326952369	40.0	36.0	41.0	34.0	41.0
29	37.98080434867703	40.0	36.0	41.0	34.0	41.0
30	37.822238976413466	40.0	36.0	41.0	33.0	41.0
31	37.76984279614215	40.0	36.0	41.0	33.0	41.0
32	37.695917669223476	40.0	36.0	41.0	33.0	41.0
33	37.644854071183374	40.0	36.0	41.0	33.0	41.0
34	37.5562671359353	40.0	36.0	41.0	33.0	41.0
35	37.4934332457993	40.0	35.0	41.0	33.0	41.0
36	37.37107564744265	40.0	35.0	41.0	33.0	41.0
37	37.33372079764322	40.0	35.0	41.0	33.0	41.0
38	37.193632877850035	40.0	35.0	41.0	33.0	41.0
39	37.15387495511257	40.0	35.0	41.0	33.0	41.0
40	37.02646273449958	39.0	35.0	41.0	32.0	41.0
41	36.90243047341558	39.0	35.0	41.0	32.0	41.0
42	36.89578287929664	39.0	35.0	41.0	32.0	41.0
43	36.822038153037624	39.0	35.0	41.0	32.0	41.0
44	36.70565760295176	39.0	35.0	41.0	31.0	41.0
45	36.666677445322435	39.0	35.0	41.0	31.0	41.0
46	36.56482425401774	39.0	35.0	41.0	31.0	41.0
47	36.48833267243155	39.0	35.0	41.0	31.0	41.0
48	36.37899455564424	38.0	35.0	41.0	31.0	41.0
49	36.31427156426093	38.0	35.0	41.0	31.0	41.0
50	36.20656045719654	38.0	35.0	40.0	31.0	41.0
51	35.11983369101451	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	13.0
10	17.0
11	13.0
12	15.0
13	16.0
14	19.0
15	38.0
16	43.0
17	97.0
18	156.0
19	264.0
20	479.0
21	756.0
22	1165.0
23	1565.0
24	2300.0
25	2997.0
26	4045.0
27	4759.0
28	5507.0
29	6026.0
30	7393.0
31	9254.0
32	11888.0
33	17021.0
34	34966.0
35	49086.0
36	40127.0
37	60872.0
38	111238.0
39	215274.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31351762565502	20.235167576895783	24.19955716743734	12.251757630011863
2	31.766003325498954	25.926638199669494	24.450246008635403	17.857112466196153
3	33.00430068365042	24.413825498101538	25.97667385432817	16.605199963919866
4	29.372460988357354	25.037058720414716	25.895323368182428	19.695156923045502
5	26.21698114813107	29.867371477294196	24.012008557118083	19.903638817456656
6	26.421718877907896	35.01866125691607	23.06286282231726	15.496757042858771
7	79.57149737653192	6.775236095108589	8.981229821930933	4.67203670642856
8	81.23084306674315	6.54548053800242	8.004683609578935	4.218992785675507
9	74.61660605091043	8.419094558877841	10.15859941012388	6.805699980087852
10	42.17120703358345	26.63037096162061	16.589882926779456	14.608539078016477
11	33.05450652761066	27.10298665205308	22.210724989405715	17.631781830930542
12	30.585400140576365	23.588917953439612	26.19332483521421	19.632357070769817
13	26.446736705237235	24.668258503933927	27.492720152625765	21.392284638203073
14	20.73399241976851	28.85134134698025	27.520971576684744	22.8936946565665
15	20.53282866532444	26.85944576152054	32.021627656442256	20.58609791671276
16	23.729664505829835	24.872145287202958	31.547990830200433	19.850199376766778
17	23.309637309579443	24.583163853153863	28.3513251789966	23.755873658270094
18	24.85393503193602	25.50048418856294	27.02844373796974	22.617137041531297
19	25.797124141182238	28.434037179554817	24.610904709308162	21.157933969954783
20	28.74395189769581	25.364332747314837	26.053429229331787	19.83828612565757
21	26.893994189737246	26.977897515406386	25.18988871321571	20.93821958164066
22	24.641198404985865	25.086583807168715	26.3755975771851	23.89662021066032
23	25.43479111816073	27.107071195290523	26.067895319964396	21.390242366584353
24	24.97408867883747	24.911459015863343	26.89978062599029	23.214671679308896
25	23.855604588984328	26.80889953895718	25.59340754721477	23.742088324843724
26	23.20599202492933	27.543266375189123	26.571315273979245	22.679426325902302
27	23.986650351185624	27.585132943372916	26.68738437764325	21.74083232779821
28	21.847030451971726	29.53431101414103	27.426176135715757	21.192482398171485
29	23.31457279932469	28.54738325439386	26.823706008192914	21.314337938088535
30	24.49704806656444	26.617606764003597	26.876975259581233	22.008369909850728
31	25.180698490931462	27.522162901795667	24.57976006712266	22.71737854015021
32	25.939742775889624	29.45142882428125	24.318008921323187	20.29081947850594
33	25.83984165587383	28.156288239408696	23.737663403003157	22.266206701714317
34	23.05894846838138	28.921799717826136	26.065682859044117	21.953568954748366
35	24.017284425466446	27.465489864376146	25.59817284765845	22.91905286249896
36	24.17402877220332	30.519026312967913	24.10391077996055	21.203034134868215
37	24.959622588204862	30.140865684901314	24.28243935729712	20.617072369596702
38	23.685755666027323	29.174360641341362	24.78160457877297	22.358279113858345
39	24.8476380277783	26.853999703870617	25.11874958516358	23.179612683187507
40	24.5526999681746	25.786232025882388	27.580708021532352	22.08035998441066
41	21.513119042310763	28.06319469145531	27.343123756554416	23.080562509679517
42	23.46280768098356	27.807229981908876	25.794571301658838	22.93539103544873
43	24.503174881420602	26.96938805032838	25.539287349318645	22.98814971893237
44	23.957377791317285	27.240329418412102	25.978716125946892	22.823576664323728
45	23.639123797399847	26.5318313560173	25.17440148677374	24.654643359809118
46	22.817960417372245	28.427059418190854	25.130492646971227	23.624487517465678
47	22.843659001907824	27.71532775906641	27.356909089980785	22.084104149044983
48	23.798420983660126	26.36028054004469	26.68244888789801	23.158849588397175
49	22.24510322832086	26.89620665065753	27.34754867839498	23.511141442626634
50	21.311955287866695	26.854850650378413	27.60657679536949	24.2266172663854
51	20.876951433079014	26.532682302525103	26.04764279307874	26.54272347131715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	831.5
2	1386.0
3	2411.0
4	3436.0
5	2327.5
6	1219.0
7	1207.0
8	1195.0
9	1209.5
10	1224.0
11	1244.0
12	1264.0
13	1295.5
14	1327.0
15	1220.5
16	1114.0
17	1226.5
18	1339.0
19	1279.5
20	1220.0
21	1673.5
22	2127.0
23	2279.0
24	2431.0
25	2956.0
26	4305.0
27	5129.0
28	6360.5
29	7592.0
30	8674.5
31	9757.0
32	11712.0
33	13667.0
34	14558.5
35	15450.0
36	17040.0
37	18630.0
38	19128.5
39	19627.0
40	22414.5
41	25202.0
42	27679.0
43	30156.0
44	33020.0
45	35884.0
46	39877.5
47	43871.0
48	44975.0
49	46079.0
50	45431.0
51	44783.0
52	41375.0
53	37967.0
54	35877.5
55	33788.0
56	33591.5
57	33395.0
58	32912.0
59	32429.0
60	32044.0
61	31659.0
62	29004.5
63	26350.0
64	22671.5
65	18993.0
66	15330.0
67	11667.0
68	9856.5
69	8046.0
70	6786.0
71	5526.0
72	4553.0
73	3580.0
74	3020.5
75	1940.0
76	1419.0
77	1142.0
78	865.0
79	587.5
80	310.0
81	241.5
82	173.0
83	113.5
84	54.0
85	36.0
86	18.0
87	12.0
88	6.0
89	6.5
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.04308411777322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28752057799089	19.086330631956525
2	8.198764637541107	4.2704223423461505
3	2.8787019025515375	2.2491082739443047
4	1.5850722073631969	1.6512067531641683
5	1.0754423769533343	1.400391814340683
6	0.8788015480640421	1.3732021583436704
7	0.7520465730088841	1.3709928512947414
8	0.7112361922679686	1.4818227186271546
9	0.6410916934846234	1.5026404410563157
>10	9.645741069999984	57.00752451161708
>50	0.28787505867166796	4.608615211515069
>100	0.052460147366135405	2.5681596216839697
>500	0.0026230073683067697	0.4628083903952359
>1k	0.0026230073683067697	0.966774279714919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1882	0.32029626553615587	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	1347	0.2292449892014888	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	1321	0.22482006736092558	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	1111	0.18908031403329922	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	813	0.1383639021683819	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	688	0.11709023947336623	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	638	0.10858077439535996	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.509465078006266E-4	0.0	0.0	0.08543502938318291	0.0
2	8.509465078006266E-4	0.0	0.0	0.4401095338344841	0.0
3	8.509465078006266E-4	0.0	0.0	0.6210207613928973	0.0
4	8.509465078006266E-4	0.0	0.0	0.8461812073569431	0.0
5	8.509465078006266E-4	0.0	0.0	1.5538283232439443	0.0
6	8.509465078006266E-4	0.0	0.0	1.9944484249831087	0.0
7	8.509465078006266E-4	0.0	0.0	2.4047748310445707	0.0
8	8.509465078006266E-4	0.0	0.0	3.010648744598617	0.0
9	8.509465078006266E-4	0.0	0.0	3.255040581638957	0.0
10	8.509465078006266E-4	0.0	0.0	3.9359679771810185	0.0
11	8.509465078006266E-4	0.0	0.0	4.558350252986397	0.0
12	8.509465078006266E-4	0.0	0.0	5.25561582147823	0.0
13	0.0010211358093607519	0.0	0.0	5.467331312619026	0.0
14	0.0010211358093607519	0.0	0.0	5.558722967556814	0.0
15	0.0010211358093607519	0.0	0.0	5.770268269396049	0.0
16	0.0010211358093607519	0.0	0.0	6.101116271628933	0.0
17	0.0010211358093607519	0.0	0.0	6.500039994485866	0.0
18	0.0010211358093607519	0.0	0.0	6.939979339018791	0.0
19	0.0010211358093607519	0.0	0.0	7.235087587924048	0.0
20	0.0010211358093607519	0.0	0.0	7.510624067149891	0.0
21	0.0010211358093607519	0.0	0.0	7.819687838783079	0.0
22	0.0010211358093607519	0.0	0.0	8.173171018123458	0.0
23	0.0010211358093607519	0.0	0.0	8.51474094635463	0.0
24	0.0010211358093607519	0.0	0.0	8.813763549195771	0.0
25	0.0010211358093607519	0.0	0.0	9.063261065282914	0.0
26	0.0010211358093607519	0.0	0.0	9.30271741257801	0.0
27	0.0010211358093607519	0.0	0.0	9.550853414252673	0.0
28	0.0010211358093607519	0.0	0.0	9.816518913988029	0.0
29	0.0010211358093607519	0.0	0.0	10.080652710009343	0.0
30	0.0010211358093607519	0.0	0.0	10.409288251321945	0.0
31	0.0010211358093607519	0.0	0.0	10.711884829495848	0.0
32	0.0010211358093607519	0.0	0.0	11.003248913766782	0.0
33	0.0010211358093607519	0.0	0.0	11.290698644101834	0.0
34	0.0010211358093607519	0.0	0.0	11.5716811809776	0.0
35	0.0010211358093607519	0.0	0.0	11.88244684562639	0.0
36	0.0010211358093607519	0.0	0.0	12.202062353956306	0.0
37	0.0011913251109208773	0.0	0.0	12.521507672984661	0.0
38	0.0013615144124810026	0.0	0.0	12.827337847888206	0.0
39	0.0013615144124810026	0.0	0.0	13.149336006439963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAC	25	3.888469E-5	45.000004	30
CGTAAGG	100	0.0	45.000004	2
ATACGTA	25	3.888469E-5	45.000004	27
GTAACGG	45	3.8380676E-10	45.000004	2
CGTGCCG	20	7.030468E-4	45.0	27
GTTTACG	20	7.030468E-4	45.0	1
TTTCGAT	20	7.030468E-4	45.0	13
AAGCGTG	20	7.030468E-4	45.0	34
TCTAGCG	35	1.2105738E-7	45.0	1
CCGTAAT	20	7.030468E-4	45.0	11
CGTAGAT	20	7.030468E-4	45.0	30
GAACGTT	20	7.030468E-4	45.0	37
CGTAATT	20	7.030468E-4	45.0	12
TATAGCG	20	7.030468E-4	45.0	1
AACTCGA	20	7.030468E-4	45.0	11
TACGTTG	20	7.030468E-4	45.0	1
TAATGCG	30	2.1636843E-6	44.999996	1
GCGTAAG	65	0.0	41.53846	1
AACGGGC	105	0.0	40.714287	4
TTTACGG	50	1.0804797E-9	40.500004	2
>>END_MODULE