##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567867
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25064671833369	33.0	31.0	34.0	30.0	34.0
2	32.27456429058213	34.0	31.0	34.0	30.0	34.0
3	32.28866266220788	34.0	31.0	34.0	30.0	34.0
4	35.90722827704374	37.0	35.0	37.0	35.0	37.0
5	35.96081829019823	37.0	35.0	37.0	35.0	37.0
6	35.89882666187681	37.0	35.0	37.0	35.0	37.0
7	36.14832874599158	37.0	35.0	37.0	35.0	37.0
8	35.98805001875439	37.0	35.0	37.0	35.0	37.0
9	37.83937788249713	39.0	38.0	39.0	35.0	39.0
10	37.50622064673594	39.0	37.0	39.0	35.0	39.0
11	37.431844076165724	39.0	37.0	39.0	35.0	39.0
12	37.210956086548435	39.0	37.0	39.0	34.0	39.0
13	37.122454729716644	39.0	37.0	39.0	34.0	39.0
14	38.088286517793776	40.0	37.0	41.0	33.0	41.0
15	38.28640861328445	40.0	37.0	41.0	33.0	41.0
16	38.12239133459067	40.0	37.0	41.0	33.0	41.0
17	38.223883409319434	40.0	37.0	41.0	34.0	41.0
18	38.14722989714141	40.0	37.0	41.0	34.0	41.0
19	38.02633363093823	40.0	36.0	41.0	34.0	41.0
20	38.16642101055353	40.0	36.0	41.0	34.0	41.0
21	38.060084491615115	40.0	36.0	41.0	34.0	41.0
22	38.16253805908778	40.0	36.0	41.0	34.0	41.0
23	38.19916635409348	40.0	36.0	41.0	34.0	41.0
24	38.13691762331673	40.0	36.0	41.0	34.0	41.0
25	37.86661313300473	40.0	35.0	41.0	34.0	41.0
26	37.93792384484395	40.0	36.0	41.0	34.0	41.0
27	37.95290622628186	40.0	36.0	41.0	34.0	41.0
28	37.847522395208735	40.0	35.0	41.0	34.0	41.0
29	37.82332834977204	40.0	35.0	41.0	34.0	41.0
30	37.661841593189955	40.0	35.0	41.0	33.0	41.0
31	37.58917316906952	40.0	35.0	41.0	33.0	41.0
32	37.522703379488505	40.0	35.0	41.0	33.0	41.0
33	37.451903350608504	40.0	35.0	41.0	33.0	41.0
34	37.33428073827146	40.0	35.0	41.0	33.0	41.0
35	37.22354530198092	40.0	35.0	41.0	33.0	41.0
36	37.10649500675334	40.0	35.0	41.0	32.0	41.0
37	37.07971760993331	40.0	35.0	41.0	32.0	41.0
38	36.93261802499529	39.0	35.0	41.0	32.0	41.0
39	36.85396052244628	39.0	35.0	41.0	32.0	41.0
40	36.74623283268794	39.0	35.0	41.0	31.0	41.0
41	36.56987287516267	39.0	35.0	41.0	31.0	41.0
42	36.59238871073685	39.0	35.0	41.0	31.0	41.0
43	36.52992337994636	39.0	35.0	41.0	31.0	41.0
44	36.40911164057781	39.0	35.0	41.0	31.0	41.0
45	36.37143908696931	39.0	35.0	41.0	31.0	41.0
46	36.250146601228806	38.0	35.0	41.0	31.0	41.0
47	36.17177085479523	38.0	35.0	41.0	31.0	41.0
48	36.06985790686904	38.0	35.0	41.0	30.0	41.0
49	36.01332706425976	38.0	35.0	40.0	30.0	41.0
50	35.89147282726413	38.0	35.0	40.0	30.0	41.0
51	34.72697128024696	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	18.0
10	20.0
11	21.0
12	18.0
13	17.0
14	27.0
15	32.0
16	45.0
17	91.0
18	148.0
19	253.0
20	438.0
21	754.0
22	1095.0
23	1672.0
24	2396.0
25	3275.0
26	4379.0
27	5085.0
28	5733.0
29	6660.0
30	7692.0
31	9859.0
32	13287.0
33	19567.0
34	39282.0
35	47831.0
36	39576.0
37	59085.0
38	103696.0
39	195646.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93012272239803	20.4760973960452	24.36538837439048	12.22839150716629
2	32.69744500032578	23.96388590990496	25.283208920398614	18.055460169370647
3	33.05351429119847	23.18025171386962	26.05152262765753	17.71471136727438
4	29.19644916855531	25.529041131109924	25.640863089420584	19.633646610914177
5	25.652485529182005	29.257202830944568	25.031917684950876	20.058393954922543
6	26.11474165605679	33.76600506808813	24.854059137086676	15.26519413876841
7	80.41900656315651	6.1253779494142115	8.721056162798684	4.734559324630591
8	82.0299471531186	5.454622297122389	8.170751249852518	4.344679299906492
9	76.16202385417712	7.520775111073544	10.246061137555095	6.071139897194238
10	44.359682813053055	24.374897643286193	17.024937177191138	14.240482366469614
11	34.0602641111387	24.796123035851707	23.000632190283994	18.142980662725602
12	30.063201418642045	23.026166338244693	27.588678334891796	19.321953908221467
13	27.3704934430069	23.330639040479547	29.4940540654766	19.80481345103695
14	22.36157410097787	25.123840617609407	31.14972343876295	21.364861842649777
15	21.177670123462008	24.605585462793226	33.922731907295194	20.294012506449572
16	27.006675858959934	24.150373238804157	29.06736964817466	19.77558125406125
17	27.035731958363492	23.777222483433622	26.979909027994232	22.207136530208658
18	27.200911481033412	22.795478518737664	29.51307260326802	20.49053739696091
19	27.095781230464176	25.14197866754011	26.54389143936873	21.218348662626987
20	26.32553044991169	27.396203688539742	26.775459746736473	19.502806114812095
21	26.61450656579798	25.326176728001453	29.252096001352427	18.80722070484814
22	25.001628902542322	23.492296611706614	30.00667409798421	21.499400387766855
23	23.74658150588078	25.612511380305598	29.694805297719363	20.946101816094263
24	24.40324935240118	25.437646491167826	28.353117895563575	21.80598626086742
25	25.240593307940063	25.62395772249488	26.903834876828554	22.231614092736503
26	22.544891673578498	25.648259187450584	27.19122611456556	24.615623024405362
27	22.749164857264113	25.34149721677787	29.527864799328015	22.381473126630002
28	20.428198856422366	26.618204614812974	28.917686711853303	24.035909816911357
29	23.005034629587563	26.959305612053523	28.253622767302904	21.782036991056003
30	24.800525475155276	24.864801088987388	28.44116668163496	21.89350675422238
31	25.542072351448493	25.783502122856234	27.264834899721237	21.40959062597404
32	26.631588030295827	26.46306265375519	26.238890444417446	20.66645887153154
33	26.103647509011797	25.215235257551505	26.132527510843207	22.548589722593494
34	23.328525869613838	25.627127478793447	28.951321348132574	22.09302530346014
35	22.751630223274113	27.702613464068172	27.63129394735035	21.91446236530737
36	24.994408902084466	28.0320920215473	25.30117087275718	21.67232820361106
37	23.307041965812417	28.9287808588983	25.989536282263277	21.774640893026007
38	23.377833189813813	28.637691572146295	26.23360751725316	21.750867720786733
39	23.172855615839623	26.292071911204562	26.548293878672297	23.98677859428352
40	25.744760656984823	24.358344471504772	26.96071439263067	22.936180478879738
41	21.8832930950381	25.40383575731641	26.871785118698572	25.84108602894692
42	22.576413138992052	26.15506800007748	27.716349074695305	23.552169786235158
43	21.802992602140993	25.199210378486512	28.43817302290853	24.559623996463962
44	22.807629251215513	24.976623047298048	27.506616866273266	24.709130835213173
45	22.670977535232723	24.9229132877945	26.81719487133431	25.588914305638465
46	22.702851195790565	26.12266604680321	26.68723486309294	24.48724789431328
47	21.434068188501882	26.514307047248742	28.906064272091882	23.145560492157493
48	21.573713563211104	26.984663662442088	27.96464665141662	23.476976122930193
49	22.981789750064717	25.496815275407798	28.238302278526483	23.283092696001003
50	21.901431144968804	25.901839691336175	28.379884726529276	23.816844437165745
51	21.66299503228749	25.684006994595563	27.462768570809715	25.190229402307228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238.0
1	669.5
2	1101.0
3	2096.5
4	3092.0
5	2143.5
6	1195.0
7	1257.5
8	1320.0
9	1273.0
10	1226.0
11	1257.5
12	1289.0
13	1393.5
14	1498.0
15	1399.0
16	1300.0
17	1432.0
18	1564.0
19	1544.0
20	1524.0
21	1633.0
22	1742.0
23	2086.5
24	2431.0
25	3023.0
26	4153.0
27	4691.0
28	5385.0
29	6079.0
30	7638.0
31	9197.0
32	9891.5
33	10586.0
34	12542.0
35	14498.0
36	16066.5
37	17635.0
38	19573.5
39	21512.0
40	22755.0
41	23998.0
42	27449.5
43	30901.0
44	32235.5
45	33570.0
46	36585.5
47	39601.0
48	42668.5
49	45736.0
50	44818.5
51	43901.0
52	41625.5
53	39350.0
54	37289.5
55	35229.0
56	34334.5
57	33440.0
58	32372.5
59	31305.0
60	28977.5
61	26650.0
62	24460.5
63	22271.0
64	19809.5
65	17348.0
66	15164.0
67	12980.0
68	11183.0
69	9386.0
70	7831.5
71	6277.0
72	5237.5
73	4198.0
74	3161.5
75	1599.0
76	1073.0
77	855.5
78	638.0
79	442.0
80	246.0
81	214.5
82	183.0
83	125.0
84	67.0
85	46.0
86	25.0
87	26.5
88	28.0
89	16.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.55628503610406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9072001549953	20.09051588655341
2	8.116973436524507	4.473472672947089
3	3.1042802145810335	2.5662729127479973
4	1.7291307988531994	1.9059368463162034
5	1.2362271466868404	1.7032913811736101
6	0.9693938747361793	1.6027736354690107
7	0.827654815878585	1.5964964412498162
8	0.7221259549555675	1.591928691737957
9	0.6294695423599198	1.5611257917734342
>10	9.561825021751263	57.87279835879304
>50	0.16106714330516905	2.740144783665918
>100	0.0320850883078026	1.6360234601495272
>500	0.0019251052984681559	0.37293271481359197
>1k	6.41701766156052E-4	0.28628642260939086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1619	0.28510196929914927	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	937	0.16500342509777818	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	630	0.11094147044994691	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.521951442855457E-4	0.0	0.0	0.07836341960353392	0.0
2	3.521951442855457E-4	0.0	0.0	0.37473563351982064	0.0
3	3.521951442855457E-4	0.0	0.0	0.5386824731847422	0.0
4	3.521951442855457E-4	0.0	0.0	0.7459493155967859	0.0
5	3.521951442855457E-4	0.0	0.0	1.3034742290008048	0.0
6	3.521951442855457E-4	0.0	0.0	1.7130771818048944	0.0
7	3.521951442855457E-4	0.0	0.0	2.0969698890761395	0.0
8	3.521951442855457E-4	0.0	0.0	2.6559035830573006	0.0
9	3.521951442855457E-4	0.0	0.0	2.9117733553807494	0.0
10	3.521951442855457E-4	0.0	0.0	3.4140036311319375	0.0
11	3.521951442855457E-4	0.0	0.0	4.102897333354465	0.0
12	3.521951442855457E-4	0.0	0.0	4.6473910264199185	0.0
13	3.521951442855457E-4	0.0	0.0	4.8564188445533905	0.0
14	3.521951442855457E-4	0.0	0.0	4.960492509689769	0.0
15	3.521951442855457E-4	0.0	0.0	5.140464228419683	0.0
16	3.521951442855457E-4	0.0	0.0	5.459905224286673	0.0
17	3.521951442855457E-4	0.0	0.0	5.825131588910784	0.0
18	3.521951442855457E-4	0.0	0.0	6.24530039604344	0.0
19	3.521951442855457E-4	0.0	0.0	6.503459436804745	0.0
20	3.521951442855457E-4	0.0	0.0	6.783454576511754	0.0
21	5.282927164283186E-4	0.0	0.0	7.111348255841596	0.0
22	5.282927164283186E-4	0.0	0.0	7.442939984186438	0.0
23	5.282927164283186E-4	0.0	0.0	7.765902931496283	0.0
24	7.043902885710914E-4	0.0	0.0	8.037269290168297	0.0
25	7.043902885710914E-4	0.0	0.0	8.2978936969396	0.0
26	7.043902885710914E-4	0.0	0.0	8.55693322556162	0.0
27	8.804878607138643E-4	0.0	0.0	8.873380562702183	0.0
28	8.804878607138643E-4	0.0	0.0	9.141753262647768	0.0
29	8.804878607138643E-4	0.0	0.0	9.45115669690262	0.0
30	8.804878607138643E-4	0.0	0.0	9.854244039537427	0.0
31	0.0010565854328566371	0.0	0.0	10.152201131602999	0.0
32	0.0010565854328566371	0.0	0.0	10.431667978593579	0.0
33	0.0010565854328566371	0.0	0.0	10.738253851694147	0.0
34	0.0010565854328566371	0.0	0.0	11.078826556218269	0.0
35	0.0010565854328566371	0.0	0.0	11.440002676683097	0.0
36	0.0010565854328566371	0.0	0.0	11.749582208510091	0.0
37	0.0010565854328566371	0.0	0.0	12.09244418147207	0.0
38	0.0010565854328566371	0.0	0.0	12.471934449439745	0.0
39	0.0010565854328566371	0.0	0.0	13.136174491562285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	25	3.888303E-5	45.000004	26
TAATACG	20	7.0302683E-4	45.0	1
TGCCCGC	20	7.0302683E-4	45.0	41
GTTTACG	20	7.0302683E-4	45.0	1
TTGTACG	20	7.0302683E-4	45.0	1
AGTTACG	20	7.0302683E-4	45.0	1
TATAGCG	20	7.0302683E-4	45.0	1
ACAACGA	120	0.0	41.249996	13
GCTACGA	100	0.0	40.500004	10
GATCTCG	45	1.9254003E-8	40.000004	9
GACCGAT	130	0.0	39.807693	9
ATATGCG	40	3.4540972E-7	39.375	1
TTGAGCG	40	3.4540972E-7	39.375	1
ACACGAC	35	6.242706E-6	38.57143	26
TACGAAT	105	0.0	38.57143	12
CGAATAT	105	0.0	38.57143	14
CGGCTAG	35	6.242706E-6	38.57143	30
AACGAGC	130	0.0	38.076927	15
TACGCTC	30	1.13919465E-4	37.499996	30
CTACGAA	110	0.0	36.81818	11
>>END_MODULE