##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513738
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91754941234637	33.0	31.0	34.0	30.0	34.0
2	31.938240893217944	33.0	31.0	34.0	30.0	34.0
3	31.969854283701032	33.0	31.0	34.0	30.0	34.0
4	35.665652531056686	37.0	35.0	37.0	33.0	37.0
5	35.67543767445663	37.0	35.0	37.0	35.0	37.0
6	35.57743246557584	37.0	35.0	37.0	33.0	37.0
7	35.81527549061973	37.0	35.0	37.0	35.0	37.0
8	35.53503342170523	37.0	35.0	37.0	33.0	37.0
9	37.31245304026566	39.0	37.0	39.0	34.0	39.0
10	37.08324087375277	39.0	37.0	39.0	33.0	39.0
11	37.06403458572268	39.0	37.0	39.0	33.0	39.0
12	36.8854221412471	39.0	35.0	39.0	33.0	39.0
13	36.70965161230043	39.0	35.0	39.0	33.0	39.0
14	37.68433131284819	40.0	36.0	41.0	32.0	41.0
15	37.69417679829018	40.0	36.0	41.0	32.0	41.0
16	37.7071347651916	40.0	36.0	41.0	33.0	41.0
17	37.58386375934815	39.0	36.0	41.0	32.0	41.0
18	37.54402633248854	39.0	36.0	41.0	33.0	41.0
19	37.43905843056188	39.0	36.0	41.0	32.0	41.0
20	37.29642346877202	39.0	35.0	41.0	32.0	41.0
21	37.38251404412366	39.0	35.0	41.0	32.0	41.0
22	37.49559697744765	39.0	35.0	41.0	33.0	41.0
23	37.49236965145658	39.0	35.0	41.0	33.0	41.0
24	37.48625174699944	39.0	35.0	41.0	32.0	41.0
25	37.37125344046966	39.0	35.0	41.0	32.0	41.0
26	37.30830306498643	39.0	35.0	41.0	32.0	41.0
27	37.23794035091817	39.0	35.0	41.0	32.0	41.0
28	37.034071842067355	39.0	35.0	41.0	31.0	41.0
29	37.06444140787717	39.0	35.0	41.0	31.0	41.0
30	36.94096212466277	39.0	35.0	41.0	31.0	41.0
31	36.88011593458144	39.0	35.0	41.0	31.0	41.0
32	36.71250715345176	39.0	35.0	41.0	31.0	41.0
33	36.59476036423235	39.0	35.0	41.0	30.0	41.0
34	36.40233153864421	39.0	35.0	41.0	30.0	41.0
35	36.34408394940612	39.0	35.0	41.0	30.0	41.0
36	36.213009354963035	39.0	35.0	41.0	30.0	41.0
37	36.127284724898686	39.0	35.0	41.0	30.0	41.0
38	35.90958620931292	39.0	35.0	41.0	29.0	41.0
39	35.81655240609027	39.0	35.0	41.0	28.0	41.0
40	35.70088255102796	38.0	35.0	41.0	27.0	41.0
41	35.47000027251245	38.0	35.0	40.0	26.0	41.0
42	35.56977681230511	38.0	35.0	41.0	27.0	41.0
43	35.557587719810485	38.0	35.0	40.0	27.0	41.0
44	35.51841794844843	38.0	35.0	40.0	27.0	41.0
45	35.497161977506046	38.0	35.0	40.0	27.0	41.0
46	35.35928819748588	38.0	34.0	40.0	27.0	41.0
47	35.040071008957874	37.0	34.0	40.0	26.0	41.0
48	34.96117865526786	37.0	34.0	40.0	26.0	41.0
49	34.97854937730906	37.0	34.0	40.0	26.0	41.0
50	34.849518626225816	37.0	34.0	40.0	26.0	41.0
51	33.51871771214121	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	10.0
10	8.0
11	10.0
12	12.0
13	17.0
14	22.0
15	21.0
16	48.0
17	106.0
18	256.0
19	620.0
20	1291.0
21	2157.0
22	3112.0
23	3730.0
24	4415.0
25	5097.0
26	5497.0
27	6013.0
28	6532.0
29	7409.0
30	9181.0
31	12136.0
32	15886.0
33	21630.0
34	36805.0
35	41965.0
36	37979.0
37	55307.0
38	92523.0
39	143856.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.838291113369074	16.976552250368865	19.657685434988263	11.527471201273801
2	29.455286546839048	33.577231974274824	20.672015696717004	16.29546578216912
3	28.161241722434394	33.6366007575846	23.464100378013697	14.738057141967307
4	25.699286406689794	21.351544950928293	35.13872830119633	17.810440341185586
5	32.428981309539104	24.740042589802584	23.15927573977397	19.67170036088434
6	22.877030704366817	38.21247406265451	24.69254756315476	14.21794766982392
7	69.83287200868925	5.546601575121949	19.76318668270597	4.857339733482826
8	68.70953676776878	14.210161599881651	11.39763848498651	5.682663147363052
9	63.33636990061082	6.855829235914027	12.492554570617708	17.31524629285745
10	43.54203893813578	21.272321689265734	19.55471466000179	15.630924712596695
11	35.48345654789017	22.063386395399988	23.6630344650386	18.790122591671242
12	27.112458101211125	19.710046755350003	28.674733035126852	24.50276210831202
13	27.861088726160027	21.524590355395162	33.251579598939536	17.36274131950527
14	21.016354639913732	29.695681456306524	28.593368604230175	20.694595299549576
15	18.034679155522852	22.943601602373196	37.16758347640236	21.85413576570158
16	22.099202317134413	24.35521608290607	26.964912075805174	26.580669524154338
17	22.5344436269071	26.481591784138995	29.220925841576832	21.763038747377067
18	23.42536468005092	22.511669372325972	29.307935173181658	24.755030774441448
19	23.043263297634205	25.102289493866525	25.96031440150427	25.894132806995007
20	26.32431317130522	25.622204314261353	29.943473132219147	18.110009382214283
21	22.8663248581962	30.509131113524795	29.007198221661625	17.617345806617383
22	21.815984022984477	21.729947950122437	32.48659822711188	23.96746979978121
23	23.21455683636406	27.544974286504015	29.01926663007214	20.221202247059786
24	23.241613429413434	25.615002199564756	26.457844270815084	24.685540100206723
25	21.472034383284864	31.798504295964094	25.077179418302713	21.65228190244833
26	20.672599651962674	23.14058917191253	29.58628717361768	26.600524002507115
27	25.438842367120984	25.38122544954821	25.944352958122625	23.23557922520818
28	18.275658020235998	27.34740276171901	29.85023494466051	24.526704273384485
29	22.905644511404645	23.688339192350966	27.932915221377435	25.473101074866953
30	24.215066823945282	26.53200658701517	28.514729297813286	20.738197291226268
31	25.479719234317884	25.117277678505385	24.723107887678157	24.679895199498578
32	28.220999809241288	25.23348477239371	27.37173423028859	19.173781188076415
33	25.85578641252934	22.985451728312874	26.954400881383116	24.204360977774662
34	25.862404571980267	22.948857199584225	28.25447990999303	22.934258318442474
35	27.556069436171743	24.470255266303056	26.168397120711333	21.80527817681386
36	23.03761839692606	31.1980036516668	23.73057862178776	22.033799329619377
37	24.669578656825074	25.937540146923137	29.203407184206736	20.18947401204505
38	21.82182357544118	28.554048951021727	26.380762178386647	23.243365295150447
39	25.532275206428178	23.918612210893492	28.364652799676097	22.184459783002232
40	26.201098614468854	21.511548688241867	29.798846883041552	22.48850581424773
41	23.44288333742102	26.708361071207502	24.38207802420689	25.466677567164588
42	24.385192452183798	23.345168159645578	29.52088418610264	22.74875520206798
43	22.926666900248765	25.71174411859742	26.06445308698208	25.297135894171735
44	23.919780121384832	22.209375206817484	29.057029069292128	24.813815602505557
45	22.256286278219637	21.65578563392235	27.430908361849816	28.657019726008198
46	26.10318878494485	25.42268627199078	25.585999089029816	22.88812585403455
47	19.360257563193688	24.761454282143816	33.98385947700968	21.89442867765281
48	22.948078592589997	25.915739151084793	26.82982376230686	24.30635849401835
49	23.50458794171348	21.350960995682623	32.476670987935485	22.66778007466841
50	22.09258415768349	23.505366548707705	29.674659067462407	24.7273902261464
51	22.177063016557078	22.355947973480646	26.6922049760773	28.774784033884977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	516.5
2	872.0
3	2063.0
4	3254.0
5	2169.5
6	1085.0
7	1063.5
8	1042.0
9	1084.5
10	1127.0
11	1090.0
12	1053.0
13	1085.0
14	1117.0
15	1068.5
16	1020.0
17	1060.5
18	1101.0
19	1149.0
20	1197.0
21	1424.0
22	1651.0
23	1953.5
24	2256.0
25	2307.0
26	3091.5
27	3825.0
28	4380.0
29	4935.0
30	5944.0
31	6953.0
32	7947.5
33	8942.0
34	10159.0
35	11376.0
36	11989.5
37	12603.0
38	14075.5
39	15548.0
40	16713.5
41	17879.0
42	20203.0
43	22527.0
44	24843.0
45	27159.0
46	52966.0
47	78773.0
48	61462.5
49	44152.0
50	44340.0
51	44528.0
52	39744.5
53	34961.0
54	33027.5
55	31094.0
56	29722.5
57	28351.0
58	27019.5
59	25688.0
60	23335.0
61	20982.0
62	19028.5
63	17075.0
64	14698.0
65	12321.0
66	10731.5
67	9142.0
68	7557.5
69	5973.0
70	5058.0
71	4143.0
72	3335.5
73	2528.0
74	2028.5
75	1118.0
76	707.0
77	540.0
78	373.0
79	265.5
80	158.0
81	148.0
82	138.0
83	94.5
84	51.0
85	33.0
86	15.0
87	9.5
88	4.0
89	4.5
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.60492993321921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10139786716474	23.419694875453413
2	8.62697989854841	5.453101904578261
3	3.270856804593443	3.101256003923071
4	1.8593697736232075	2.3506100566122856
5	1.3158521404001458	2.0793707349911568
6	1.0583494530038065	2.006943618422771
7	0.943316608371785	2.0869418718702577
8	0.7923060204306642	2.0032621009103115
9	0.7292573849104707	2.074331569804029
>10	7.187426914300674	41.46231679984714
>50	0.06979702266533644	1.455523377093857
>100	0.03520734771614316	1.8742596490716363
>500	0.0030883638347494	0.6340584668456073
>1k	0.00494138213559904	3.3686308201624993
>5k	0.0	0.0
>10k+	0.0018530183008496398	6.629698150413691
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	11649	2.2674982189365007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	11328	2.205015007649814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	10984	2.138054806146324	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	3066	0.5968022610747112	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	2512	0.4889651923743231	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT	2320	0.45159205665144486	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT	2175	0.4233675531107296	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT	2032	0.39553235306712764	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1799	0.35017849565342646	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	1748	0.3402512564770369	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	1604	0.3122214046848783	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	846	0.16467537927893205	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	731	0.14229042819491647	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	583	0.1134819694085312	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	557	0.10842102394605811	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	531	0.10336007848358501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.839552456699718E-4	0.0	0.0	0.1535802296112026	0.0
2	5.839552456699718E-4	0.0	0.0	1.0857674534490342	0.0
3	5.839552456699718E-4	0.0	0.0	1.3717108720787639	0.0
4	5.839552456699718E-4	0.0	0.0	2.1754279418692017	0.0
5	5.839552456699718E-4	0.0	0.0	5.347083532851376	0.0
6	5.839552456699718E-4	0.0	0.0	6.37348220299063	0.0
7	5.839552456699718E-4	0.0	0.0	7.287761465961249	0.0
8	5.839552456699718E-4	0.0	0.0	8.304232896924113	0.0
9	5.839552456699718E-4	0.0	0.0	8.590760270799512	0.0
10	5.839552456699718E-4	0.0	0.0	11.34702903036178	0.0
11	5.839552456699718E-4	0.0	0.0	12.221793988375397	0.0
12	5.839552456699718E-4	0.0	0.0	15.152860018141544	0.0
13	5.839552456699718E-4	0.0	0.0	15.581483168463302	0.0
14	5.839552456699718E-4	0.0	0.0	15.825381809404794	0.0
15	5.839552456699718E-4	0.0	0.0	16.405833323600746	0.0
16	5.839552456699718E-4	0.0	0.0	16.89110013275249	0.0
17	5.839552456699718E-4	0.0	0.0	17.400893062222377	0.0
18	5.839552456699718E-4	0.0	0.0	17.92139183786288	0.0
19	5.839552456699718E-4	0.0	0.0	18.73367358458981	0.0
20	5.839552456699718E-4	0.0	0.0	19.161518127917343	0.0
21	5.839552456699718E-4	0.0	0.0	19.59500757195302	0.0
22	5.839552456699718E-4	0.0	0.0	20.15988694626444	0.0
23	5.839552456699718E-4	0.0	0.0	20.549579746874866	0.0
24	7.786069942266291E-4	0.0	0.0	20.915525034161384	0.0
25	9.732587427832864E-4	0.0	0.0	21.24584905146203	0.0
26	9.732587427832864E-4	0.0	0.0	21.632232772346995	0.0
27	9.732587427832864E-4	0.0	0.0	22.111076073796372	0.0
28	9.732587427832864E-4	0.0	0.0	22.432251458914855	0.0
29	9.732587427832864E-4	0.0	0.0	22.77367062588323	0.0
30	9.732587427832864E-4	0.0	0.0	23.204240293690557	0.0
31	0.0011679104913399436	0.0	0.0	23.56181555578914	0.0
32	0.0011679104913399436	0.0	0.0	23.90946357871133	0.0
33	0.001362562239896601	0.0	0.0	24.259447422616198	0.0
34	0.001362562239896601	0.0	0.0	24.611183132258077	0.0
35	0.001362562239896601	0.0	0.0	24.960583020917277	0.0
36	0.001362562239896601	0.0	0.0	25.30219683963421	0.0
37	0.001362562239896601	0.0	0.0	25.64809299681939	0.0
38	0.001362562239896601	0.0	0.0	26.029999727487553	0.0
39	0.001362562239896601	0.0	0.0	26.622519650094016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCA	20	7.0296385E-4	45.000004	25
GCGTTAG	20	7.0296385E-4	45.000004	1
TTATACG	20	7.0296385E-4	45.000004	26
GTGTCGT	20	7.0296385E-4	45.000004	1
CGATCGA	85	0.0	45.000004	41
TAAGACG	20	7.0296385E-4	45.000004	1
ACTCAAC	35	1.2101737E-7	45.0	45
CGGGTAT	65	0.0	44.999996	6
CCGATGA	1040	0.0	42.403843	18
CGATGAA	1085	0.0	41.8894	19
CCGATTA	60	3.6379788E-12	41.249996	18
AGTACGG	60	3.6379788E-12	41.249996	2
TACGGCT	1355	0.0	41.180813	7
ACGGCTG	1360	0.0	41.029415	8
ACGTTGG	50	1.0786607E-9	40.5	2
TACGGAA	50	1.0786607E-9	40.5	5
GTTCCGA	50	1.0786607E-9	40.5	15
CCGATCG	90	0.0	40.0	40
CGTTAGG	45	1.9246727E-8	40.0	2
AGGGCGC	45	1.9246727E-8	40.0	6
>>END_MODULE