##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	807217
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11745168895105	33.0	31.0	34.0	30.0	34.0
2	32.13599193277644	33.0	31.0	34.0	30.0	34.0
3	32.14486439210274	33.0	31.0	34.0	30.0	34.0
4	35.796925733724635	37.0	35.0	37.0	35.0	37.0
5	35.86066571937905	37.0	35.0	37.0	35.0	37.0
6	35.80195412138248	37.0	35.0	37.0	35.0	37.0
7	36.06315897707803	37.0	35.0	37.0	35.0	37.0
8	35.879257993823224	37.0	35.0	37.0	35.0	37.0
9	37.6925981489488	39.0	38.0	39.0	35.0	39.0
10	37.372042461940225	39.0	37.0	39.0	34.0	39.0
11	37.31625572801366	39.0	37.0	39.0	34.0	39.0
12	37.14614905285691	39.0	37.0	39.0	34.0	39.0
13	37.04645838727381	39.0	37.0	39.0	33.0	39.0
14	38.06712073705088	40.0	37.0	41.0	33.0	41.0
15	38.1969569520959	40.0	37.0	41.0	33.0	41.0
16	38.04407984470099	40.0	37.0	41.0	33.0	41.0
17	38.11047091426469	40.0	37.0	41.0	33.0	41.0
18	38.035237117156846	40.0	37.0	41.0	33.0	41.0
19	37.8986307275491	40.0	36.0	41.0	33.0	41.0
20	38.01039125786499	40.0	36.0	41.0	34.0	41.0
21	37.908704846404376	40.0	36.0	41.0	33.0	41.0
22	38.02932792545251	40.0	36.0	41.0	34.0	41.0
23	38.061266053613835	40.0	36.0	41.0	34.0	41.0
24	37.99409576359269	40.0	36.0	41.0	34.0	41.0
25	37.75515381861383	40.0	35.0	41.0	33.0	41.0
26	37.823792115379135	40.0	35.0	41.0	34.0	41.0
27	37.83432955450641	40.0	36.0	41.0	34.0	41.0
28	37.71007672038622	40.0	35.0	41.0	33.0	41.0
29	37.663363135315535	40.0	35.0	41.0	33.0	41.0
30	37.50714244125186	40.0	35.0	41.0	33.0	41.0
31	37.45120333194172	40.0	35.0	41.0	33.0	41.0
32	37.37433924211209	40.0	35.0	41.0	33.0	41.0
33	37.2841069997287	40.0	35.0	41.0	33.0	41.0
34	37.12278111090326	40.0	35.0	41.0	32.0	41.0
35	37.03025580482076	40.0	35.0	41.0	32.0	41.0
36	36.93099748890323	39.0	35.0	41.0	32.0	41.0
37	36.92382097998431	39.0	35.0	41.0	32.0	41.0
38	36.75003871325802	39.0	35.0	41.0	31.0	41.0
39	36.68055306070115	39.0	35.0	41.0	31.0	41.0
40	36.55858585733452	39.0	35.0	41.0	31.0	41.0
41	36.38839494212832	39.0	35.0	41.0	31.0	41.0
42	36.419511729807475	39.0	35.0	41.0	31.0	41.0
43	36.343207588541866	39.0	35.0	41.0	31.0	41.0
44	36.23677648017819	38.0	35.0	41.0	31.0	41.0
45	36.204191685754886	38.0	35.0	41.0	31.0	41.0
46	36.08578362447768	38.0	35.0	40.0	30.0	41.0
47	35.996251317799306	38.0	35.0	40.0	30.0	41.0
48	35.89747738216614	38.0	35.0	40.0	30.0	41.0
49	35.86946508807421	38.0	35.0	40.0	30.0	41.0
50	35.73889053377221	37.0	35.0	40.0	30.0	41.0
51	34.57799080049107	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	12.0
9	16.0
10	19.0
11	21.0
12	34.0
13	20.0
14	30.0
15	50.0
16	77.0
17	147.0
18	255.0
19	414.0
20	720.0
21	1247.0
22	1779.0
23	2628.0
24	3662.0
25	5200.0
26	6744.0
27	7950.0
28	8806.0
29	9767.0
30	11749.0
31	15003.0
32	19744.0
33	28841.0
34	55572.0
35	67974.0
36	61121.0
37	90818.0
38	150985.0
39	255650.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18120158519952	19.944451120330715	23.044856587509926	13.829490706959838
2	31.41683091411603	25.530309693675925	24.874971661895128	18.177887730312914
3	32.137207219372236	25.01322444894	26.183665606646045	16.665902725041718
4	28.40983279589008	24.146171351693535	27.717825566111713	19.726170286304672
5	26.0449172898985	28.257952942021785	25.630654458466555	20.066475309613153
6	25.471589423909556	33.57288065043228	25.60525856120473	15.350271364453425
7	77.81253368053449	5.9442504308011355	11.34106442257782	4.902151466086567
8	78.63176816147332	6.394563048102307	9.839981070765358	5.133687719659026
9	72.92772575404135	6.908551232196547	11.840186715591965	8.323536298170133
10	43.55198168522219	23.06467777561672	18.41933457793877	14.964005961222323
11	32.77012253210722	23.952914767652317	23.756685005395077	19.520277694845376
12	28.268235183352186	21.407750332314606	29.15721546994179	21.166799014391422
13	26.59235372892295	22.68262437485831	31.17612736104418	19.548894535174558
14	21.379257374411097	25.781419370503844	31.366039119592376	21.473284135492687
15	20.04578694452669	23.494673675108427	35.26796388083997	21.191575499524912
16	24.253577414747213	24.316385804560607	29.677769422596402	21.75226735809578
17	24.656690827869085	24.00420209187864	27.802189497991247	23.536917582261026
18	26.58231925244389	22.714833805531846	29.05464082148914	21.64820612053512
19	25.45969671104548	25.227912692621686	26.671019069221785	22.641371527111048
20	27.309137443834807	25.312896036629557	27.155523236007173	20.22244328352847
21	25.4150990378052	26.576001248734848	28.688692136067502	19.32020757739245
22	24.48127331312398	22.562830069237886	29.257312469881086	23.698584147757046
23	23.3634821863266	25.163246066423277	29.01338797374188	22.45988377350824
24	23.79013326032529	23.532705579788335	28.839828695381787	23.837332464504588
25	23.977691252785807	26.075392366612693	26.631252810582534	23.315663570018966
26	22.780367608709927	24.337445816924074	27.087759549167078	25.794427025198924
27	22.78061537356126	25.01087068285232	28.067917301047924	24.1405966425385
28	20.520752164535683	25.69160461189494	28.82310456791668	24.964538655652692
29	22.803781387161074	26.45918012133045	26.831322928035462	23.905715563473017
30	23.32470698709269	24.59202420167068	28.25200658558975	23.83126222564688
31	24.012130567121357	24.472477660901593	26.83664987233916	24.678741899637892
32	24.989562905637516	25.561775829795458	25.830724575919483	23.61793668864754
33	23.985371963177187	25.10403026695424	25.502931677603417	25.407666092265153
34	21.43042081621175	25.680826840861876	28.610893972748343	24.27785837017803
35	21.082187317660555	26.72973933898815	26.26666683184323	25.92140651150806
36	22.63108928578065	28.841934696618132	25.378429839807637	23.14854617779358
37	21.44999423946721	26.732588634778505	25.959810063464968	25.857607062289322
38	21.382230552627114	27.70506567626797	25.50243614790075	25.41026762320417
39	22.431514698030394	25.25516682626853	25.87507448430843	26.438243991392653
40	24.09005261286618	23.947587823348616	27.384829605917616	24.577529957867586
41	20.9149460430095	24.409049858959857	26.750799351351617	27.92520474667902
42	21.929171461948894	24.980643370989462	27.440081167765296	25.65010399929635
43	22.483793081662057	24.579140429401264	27.485917665262257	25.451148823674423
44	22.08997085046524	23.875736016461495	28.042273638934763	25.992019494138503
45	22.231444580577467	23.408947036546554	27.003023970010542	27.356584412865438
46	22.575589959081636	24.84486823245794	26.51975862748183	26.059783180978595
47	20.203365389975684	25.563757948606135	29.338827105970267	24.894049555447918
48	21.24843753290627	25.52907086941925	27.926195806084365	25.29629579159012
49	23.034822111030863	23.294975204932502	29.09031896008137	24.579883723955266
50	20.87381707768791	24.662141654598454	28.63418386877382	25.829857398939815
51	21.117246044124443	23.748137118024026	27.13087063329935	28.003746204552183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	386.0
1	932.0
2	1478.0
3	4184.0
4	6890.0
5	4435.0
6	1980.0
7	1924.5
8	1869.0
9	1893.0
10	1917.0
11	1943.5
12	1970.0
13	2007.0
14	2044.0
15	1965.0
16	1886.0
17	1884.0
18	1882.0
19	1998.0
20	2114.0
21	2171.0
22	2228.0
23	2609.5
24	2991.0
25	3242.0
26	4375.5
27	5258.0
28	6272.5
29	7287.0
30	8936.5
31	10586.0
32	12010.0
33	13434.0
34	15974.5
35	18515.0
36	19084.5
37	19654.0
38	22210.0
39	24766.0
40	27094.5
41	29423.0
42	33672.5
43	37922.0
44	40318.0
45	42714.0
46	57249.0
47	71784.0
48	71397.0
49	71010.0
50	70981.0
51	70952.0
52	64084.5
53	57217.0
54	53467.5
55	49718.0
56	48954.0
57	48190.0
58	45819.0
59	43448.0
60	40462.0
61	37476.0
62	34838.5
63	32201.0
64	29203.5
65	26206.0
66	22813.0
67	19420.0
68	16329.0
69	13238.0
70	11088.5
71	8939.0
72	7651.5
73	6364.0
74	5119.5
75	3150.0
76	2425.0
77	1717.5
78	1010.0
79	802.0
80	594.0
81	416.5
82	239.0
83	193.0
84	147.0
85	89.5
86	32.0
87	29.0
88	26.0
89	16.0
90	6.0
91	6.5
92	7.0
93	5.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	807217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.349554452113196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3907495966796	21.539858015691525
2	9.428568259938075	5.534485551010374
3	3.4331699961478175	3.0228602923590486
4	1.8155370155242663	2.1314080998782616
5	1.2213751325818196	1.7923407980083548
6	0.8965905232637086	1.5788719430266127
7	0.7517764840665431	1.5445013398450451
8	0.6354459032095954	1.4920043310097817
9	0.5253076734826027	1.3875791550291492
>10	7.644121381966973	50.166201029670276
>50	0.19969626634842885	3.561021977861842
>100	0.04960607878307065	2.714972825666442
>500	0.004663819372767326	0.9103832245199234
>1k	0.0033918686347398736	2.623511416423393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4279	0.530092899431008	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	4232	0.524270425424638	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	3702	0.45861273982089085	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	3279	0.4062104737635605	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	2037	0.2523485010845906	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1374	0.17021445286707293	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	1158	0.14345584892290425	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	1022	0.12660783903213138	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	949	0.1175644219584077	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	931	0.11533453829639367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7164727700234263E-4	0.0	0.0	0.14977385263194407	0.0
2	3.7164727700234263E-4	0.0	0.0	0.7154210082295095	0.0
3	3.7164727700234263E-4	0.0	0.0	1.038878021647215	0.0
4	3.7164727700234263E-4	0.0	0.0	1.5256120720946165	0.0
5	3.7164727700234263E-4	0.0	0.0	2.9658691529043617	0.0
6	3.7164727700234263E-4	0.0	0.0	3.845434375143239	0.0
7	3.7164727700234263E-4	0.0	0.0	4.619327888287784	0.0
8	3.7164727700234263E-4	0.0	0.0	5.675301684677106	0.0
9	3.7164727700234263E-4	0.0	0.0	6.136515955437014	0.0
10	3.7164727700234263E-4	0.0	0.0	7.313027352000763	0.0
11	3.7164727700234263E-4	0.0	0.0	8.342242544445917	0.0
12	3.7164727700234263E-4	0.0	0.0	9.623310708272992	0.0
13	3.7164727700234263E-4	0.0	0.0	10.015150820659128	0.0
14	3.7164727700234263E-4	0.0	0.0	10.208655169551681	0.0
15	3.7164727700234263E-4	0.0	0.0	10.517246291889293	0.0
16	3.7164727700234263E-4	0.0	0.0	11.009307286640395	0.0
17	3.7164727700234263E-4	0.0	0.0	11.545098777652106	0.0
18	4.955297026697902E-4	0.0	0.0	12.197339748791217	0.0
19	4.955297026697902E-4	0.0	0.0	12.671190026969205	0.0
20	4.955297026697902E-4	0.0	0.0	13.10093816160958	0.0
21	4.955297026697902E-4	0.0	0.0	13.591884214529674	0.0
22	4.955297026697902E-4	0.0	0.0	14.114048638717966	0.0
23	4.955297026697902E-4	0.0	0.0	14.597190098821011	0.0
24	4.955297026697902E-4	0.0	0.0	15.020248582475345	0.0
25	6.194121283372377E-4	0.0	0.0	15.394497390416703	0.0
26	7.432945540046853E-4	0.0	0.0	15.748801127825603	0.0
27	7.432945540046853E-4	0.0	0.0	16.15909972163619	0.0
28	7.432945540046853E-4	0.0	0.0	16.51972146275413	0.0
29	7.432945540046853E-4	0.0	0.0	16.9118093399916	0.0
30	8.671769796721328E-4	0.0	0.0	17.43558423571357	0.0
31	8.671769796721328E-4	0.0	0.0	17.843776828287808	0.0
32	8.671769796721328E-4	0.0	0.0	18.247633535963686	0.0
33	8.671769796721328E-4	0.0	0.0	18.650746949085562	0.0
34	8.671769796721328E-4	0.0	0.0	19.055842481018114	0.0
35	9.910594053395803E-4	0.0	0.0	19.479520376800785	0.0
36	9.910594053395803E-4	0.0	0.0	19.85500800899882	0.0
37	9.910594053395803E-4	0.0	0.0	20.260846835485378	0.0
38	9.910594053395803E-4	0.0	0.0	20.69517861987545	0.0
39	9.910594053395803E-4	0.0	0.0	21.326359578651093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAT	20	7.032036E-4	45.000004	8
ATATGCG	20	7.032036E-4	45.000004	1
CCGTAAG	20	7.032036E-4	45.000004	1
ATAACGC	30	2.1646956E-6	45.000004	11
TACCCGT	25	3.8897706E-5	45.0	34
GTTACCG	50	2.1827873E-11	45.0	38
ATACCCG	25	3.8897706E-5	45.0	33
CGTTTTT	2005	0.0	41.857853	1
CGATGAA	255	0.0	40.588234	19
CACAACG	245	0.0	39.489796	12
AACGAGC	255	0.0	38.82353	15
TTGTACG	35	6.2459767E-6	38.57143	1
TAACGCC	35	6.2459767E-6	38.57143	12
TATAGCG	35	6.2459767E-6	38.57143	1
TACGTAG	35	6.2459767E-6	38.57143	1
ACAACGA	255	0.0	37.941177	13
CGTTAGG	60	1.546141E-10	37.500004	2
CGTAAGG	90	0.0	37.5	2
TACGGCT	505	0.0	37.425743	7
TTCACGG	55	2.746674E-9	36.81818	2
>>END_MODULE