SRR2935596.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935596.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540690
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2708	0.5008415173204609	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	2199	0.40670254674582473	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	2118	0.39172168895300447	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	2028	0.3750762914054264	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1942	0.3591706893044073	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1501	0.27760824132127465	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	977	0.18069503782204221	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	872	0.16127540734986776	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	631	0.11670273169468641	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	596	0.11022952153729494	TruSeq Adapter, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	35	1.2103374E-7	45.0	1
TTAGCCG	25	3.888055E-5	45.0	1
TAGAACG	20	7.02997E-4	45.0	1
GCACGCG	25	3.888055E-5	45.0	1
TAACGCG	20	7.02997E-4	45.0	1
AACGGTT	20	7.02997E-4	45.0	22
GTTACGG	25	3.888055E-5	45.0	2
ATGCGAG	20	7.02997E-4	45.0	1
CGTCGAG	25	3.888055E-5	45.0	17
TTACGAG	20	7.02997E-4	45.0	1
CCGCGAA	20	7.02997E-4	45.0	35
TTCGCAA	20	7.02997E-4	45.0	14
AATACGG	40	6.8030204E-9	45.0	2
GCTACGA	60	0.0	44.999996	10
CTAGGCG	30	2.1633605E-6	44.999996	1
CGCGCAA	60	3.6379788E-12	41.249996	18
AACGACG	60	3.6379788E-12	41.249996	13
ACGCGCA	60	3.6379788E-12	41.249996	17
GGCCGAT	55	6.002665E-11	40.909092	8
CGGGAGT	110	0.0	40.909092	6