##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935596.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540690
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.032802530100426	33.0	31.0	34.0	30.0	34.0
2	32.04427490798794	33.0	31.0	34.0	30.0	34.0
3	32.06651315911151	33.0	31.0	34.0	30.0	34.0
4	35.727063566923746	37.0	35.0	37.0	33.0	37.0
5	35.813628881614235	37.0	35.0	37.0	35.0	37.0
6	35.75390519521353	37.0	35.0	37.0	35.0	37.0
7	36.024429895134	37.0	35.0	37.0	35.0	37.0
8	35.81393774621317	37.0	35.0	37.0	35.0	37.0
9	37.592117479516915	39.0	37.0	39.0	35.0	39.0
10	37.30170707799294	39.0	37.0	39.0	34.0	39.0
11	37.26896558101685	39.0	37.0	39.0	34.0	39.0
12	37.09985943886515	39.0	37.0	39.0	34.0	39.0
13	36.99491575579352	39.0	37.0	39.0	33.0	39.0
14	38.05062975087388	40.0	37.0	41.0	33.0	41.0
15	38.14883389742736	40.0	37.0	41.0	33.0	41.0
16	37.950677837577906	40.0	36.0	41.0	33.0	41.0
17	37.97925058721263	40.0	36.0	41.0	33.0	41.0
18	37.910014980857795	40.0	36.0	41.0	33.0	41.0
19	37.795890436294364	40.0	36.0	41.0	33.0	41.0
20	37.977643381604985	40.0	36.0	41.0	34.0	41.0
21	37.87763598365052	40.0	36.0	41.0	33.0	41.0
22	37.98984445800736	40.0	36.0	41.0	34.0	41.0
23	38.030819878303646	40.0	36.0	41.0	34.0	41.0
24	37.95699938966876	40.0	36.0	41.0	34.0	41.0
25	37.712730030146666	40.0	35.0	41.0	33.0	41.0
26	37.803462242689896	40.0	35.0	41.0	34.0	41.0
27	37.819476964619284	40.0	35.0	41.0	34.0	41.0
28	37.71714290998539	40.0	35.0	41.0	33.0	41.0
29	37.71834137860881	40.0	36.0	41.0	33.0	41.0
30	37.55402541197359	40.0	35.0	41.0	33.0	41.0
31	37.54250124840482	40.0	35.0	41.0	33.0	41.0
32	37.50113188703324	40.0	35.0	41.0	33.0	41.0
33	37.46752852836191	40.0	35.0	41.0	33.0	41.0
34	37.39583125265864	40.0	35.0	41.0	33.0	41.0
35	37.31400987626921	40.0	35.0	41.0	33.0	41.0
36	37.22116924670328	40.0	35.0	41.0	33.0	41.0
37	37.222519373393254	40.0	35.0	41.0	33.0	41.0
38	37.09306071871128	39.0	35.0	41.0	32.0	41.0
39	37.046571972849506	39.0	35.0	41.0	32.0	41.0
40	36.93153747988681	39.0	35.0	41.0	32.0	41.0
41	36.81762377702565	39.0	35.0	41.0	31.0	41.0
42	36.82794207401653	39.0	35.0	41.0	32.0	41.0
43	36.77815753944034	39.0	35.0	41.0	32.0	41.0
44	36.668721448519484	39.0	35.0	41.0	31.0	41.0
45	36.65626329319943	39.0	35.0	41.0	31.0	41.0
46	36.52020936211137	39.0	35.0	41.0	31.0	41.0
47	36.44123064972535	38.0	35.0	41.0	31.0	41.0
48	36.3649225989014	38.0	35.0	40.0	31.0	41.0
49	36.35263274704544	38.0	35.0	40.0	31.0	41.0
50	36.21253398435333	38.0	35.0	40.0	31.0	41.0
51	35.077700715752094	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	21.0
10	9.0
11	15.0
12	12.0
13	8.0
14	20.0
15	22.0
16	52.0
17	85.0
18	144.0
19	237.0
20	396.0
21	669.0
22	1112.0
23	1452.0
24	2041.0
25	2849.0
26	3554.0
27	4524.0
28	5143.0
29	6256.0
30	7585.0
31	9901.0
32	13034.0
33	18656.0
34	38240.0
35	49717.0
36	37725.0
37	56596.0
38	101625.0
39	178866.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.026891564482426	20.617359300153506	23.360150918271096	11.995598217092972
2	32.270062327766375	25.311361408570534	23.95032273576356	18.468253527899535
3	32.408589025134546	24.564907802992472	25.684773160221198	17.34173001165178
4	28.913610386728067	24.145073887070225	27.230020899221362	19.711294826980343
5	26.86030812480349	28.376148994802936	24.676431966561246	20.087110913832326
6	25.271782352179624	33.414525883593186	25.160813035195766	16.15287872903142
7	76.56143076439365	6.344485749690211	11.39673380310344	5.697349682812702
8	76.01620152027965	7.15789084318186	10.226007508923782	6.599900127614715
9	70.14555475411048	7.892507721614973	12.53084022267843	9.43109730159611
10	42.07956500027743	23.99619005345022	18.814662745750798	15.109582200521556
11	33.365884332981935	24.02411733155782	23.576910984112892	19.03308735134735
12	30.39024209805989	20.90495478000333	28.303464092178515	20.401339029758272
13	25.791118755664062	23.209972442619616	30.977639682627757	20.021269119088572
14	21.23693798664669	27.37797998853317	28.91046625608019	22.474615768739945
15	19.390223603173723	24.518115740997615	35.18633597810206	20.905324677726608
16	22.53731343283582	24.030405592853576	31.622001516580667	21.810279457729937
17	22.354029110950822	25.295085908746234	28.19120013316318	24.159684847139765
18	23.68658565906527	25.06242024080342	29.472895744326692	21.77809835580462
19	24.593759825408274	27.07503375316725	25.99012373078844	22.34108269063604
20	26.987367992750006	26.4728402596682	27.37557565333185	19.164216094249937
21	24.494442286707727	28.743087536296212	26.931328487673156	19.8311416893229
22	22.660119476964617	25.324862675470232	27.82167230760695	24.1933455399582
23	22.804009691320353	26.257004938134603	28.342488301984503	22.59649706856054
24	24.549372098614732	23.255839760306277	28.76694593944774	23.42784220163125
25	22.106197636353546	25.900608481754794	26.319702602230482	25.673491279661175
26	20.54670883500712	25.72028334165603	28.12480349183451	25.60820433150234
27	22.012798461225472	25.89006639664133	28.307902864857866	23.78923227727533
28	18.703878377628584	26.86012317594185	29.837614899480293	24.598383546949268
29	21.556159721836913	24.797203573212006	27.912112300948788	25.734524404002297
30	21.664354805896167	24.549002200891454	31.39950803602804	22.387134957184337
31	23.782019271671384	24.34888753259724	28.32843218849988	23.5406610072315
32	24.809965044665148	24.26843477778394	27.221698200447577	23.69990197710333
33	23.1154635743217	23.97288649688361	29.0556511124674	23.855998816327286
34	21.029980210471805	23.423033531228615	31.56392757402578	23.983058684273797
35	20.97005677930052	23.558970944533836	29.849266677763598	25.621705598402038
36	21.16277349312915	26.211507518171224	29.387079472525844	23.23863951617378
37	21.917179899757716	26.02581886108491	30.270950082302246	21.78605115685513
38	21.991529342136896	26.258114631304448	26.944274907987943	24.806081118570717
39	23.150049011448335	23.41767001424106	28.50894227746028	24.92333869685032
40	23.447261832103422	22.89574432669367	30.612920527474152	23.044073313728756
41	20.175701418557768	24.42212728180658	28.257966672215133	27.14420462742052
42	21.38878100205293	23.42710240618469	28.326582699883485	26.857533891878894
43	22.33202019641569	22.48941167767112	28.38780077308624	26.790767352826943
44	21.16462298174555	22.67694982337384	29.15626329319943	27.002163901681186
45	21.853002644768722	22.115075181712257	28.565536629122047	27.466385544396978
46	22.694889862952895	23.574136751188295	29.213597440307755	24.517375945551056
47	20.31089903641643	23.224768351550797	32.05515175054098	24.409180861491798
48	20.919380791211232	21.888327877341915	30.785477815384045	26.40681351606281
49	21.6114594314672	20.85631322939207	32.25656106086667	25.275666278274056
50	20.024413249736448	21.790120031811206	31.712811407645784	26.472655310806566
51	19.71147977584198	21.61941223251771	28.89030683016146	29.778801161478853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	237.0
1	662.0
2	1087.0
3	1641.0
4	2195.0
5	1581.5
6	968.0
7	943.0
8	918.0
9	916.5
10	915.0
11	919.0
12	923.0
13	931.0
14	939.0
15	921.5
16	904.0
17	940.5
18	977.0
19	1041.5
20	1106.0
21	1369.0
22	1632.0
23	1672.5
24	1713.0
25	2305.5
26	3392.5
27	3887.0
28	4607.5
29	5328.0
30	6720.5
31	8113.0
32	9058.5
33	10004.0
34	11044.5
35	12085.0
36	13016.5
37	13948.0
38	15208.0
39	16468.0
40	18641.0
41	20814.0
42	23779.0
43	26744.0
44	30636.5
45	34529.0
46	43498.0
47	52467.0
48	54157.0
49	55847.0
50	55743.0
51	55639.0
52	48269.0
53	40899.0
54	36209.5
55	31520.0
56	29537.0
57	27554.0
58	26487.0
59	25420.0
60	25189.5
61	24959.0
62	22430.5
63	19902.0
64	17403.0
65	14904.0
66	11389.0
67	7874.0
68	6555.0
69	5236.0
70	4419.0
71	3602.0
72	2961.5
73	2321.0
74	1934.5
75	1204.5
76	861.0
77	649.5
78	438.0
79	305.5
80	173.0
81	115.5
82	58.0
83	61.5
84	65.0
85	48.0
86	31.0
87	31.5
88	32.0
89	17.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.137414886185432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1303761885546	21.738230730911006
2	10.784540858004647	6.500363643894085
3	3.821791483479982	3.455367466383789
4	1.8744942640850977	2.259696453540297
5	1.164032565284521	1.7540466180505163
6	0.9062821884677594	1.6387801390687777
7	0.6662670207713192	1.405569594097758
8	0.603459561044161	1.4549368926578579
9	0.5426399406963377	1.471838852392253
>10	7.2401528308215335	48.23062093538772
>50	0.1982410134500016	3.802324469851645
>100	0.061565532127329695	3.398954860840887
>500	0.0024626212850931877	0.5707290328045508
>1k	0.0036939319276397815	2.3185403101188773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2708	0.5008415173204609	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	2199	0.40670254674582473	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	2118	0.39172168895300447	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	2028	0.3750762914054264	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1942	0.3591706893044073	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1501	0.27760824132127465	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	977	0.18069503782204221	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	872	0.16127540734986776	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	631	0.11670273169468641	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	596	0.11022952153729494	TruSeq Adapter, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.849488616397566E-4	0.0	0.0	0.28999981505113837	0.0
2	1.849488616397566E-4	0.0	0.0	1.4838447169357674	0.0
3	1.849488616397566E-4	0.0	0.0	2.007804841961198	0.0
4	1.849488616397566E-4	0.0	0.0	2.7535186520926964	0.0
5	1.849488616397566E-4	0.0	0.0	4.75632987478962	0.0
6	1.849488616397566E-4	0.0	0.0	5.733599659694095	0.0
7	1.849488616397566E-4	0.0	0.0	6.766724000813775	0.0
8	1.849488616397566E-4	0.0	0.0	8.162163161885738	0.0
9	1.849488616397566E-4	0.0	0.0	8.706837559414822	0.0
10	1.849488616397566E-4	0.0	0.0	10.200669514879136	0.0
11	1.849488616397566E-4	0.0	0.0	11.859105957202834	0.0
12	1.849488616397566E-4	0.0	0.0	13.417670014241063	0.0
13	1.849488616397566E-4	0.0	0.0	13.940520446096654	0.0
14	1.849488616397566E-4	0.0	0.0	14.161164438032884	0.0
15	1.849488616397566E-4	0.0	0.0	14.618173075144723	0.0
16	1.849488616397566E-4	0.0	0.0	15.423995265309141	0.0
17	3.698977232795132E-4	0.0	0.0	16.288446244613365	0.0
18	3.698977232795132E-4	0.0	0.0	17.216334683460023	0.0
19	5.548465849192698E-4	0.0	0.0	17.900090624942205	0.0
20	5.548465849192698E-4	0.0	0.0	18.498400192346818	0.0
21	5.548465849192698E-4	0.0	0.0	19.20952856535168	0.0
22	5.548465849192698E-4	0.0	0.0	19.9385969779356	0.0
23	5.548465849192698E-4	0.0	0.0	20.611255987719396	0.0
24	5.548465849192698E-4	0.0	0.0	21.138175294531063	0.0
25	7.397954465590264E-4	0.0	0.0	21.61071963602064	0.0
26	7.397954465590264E-4	0.0	0.0	22.08844254563613	0.0
27	7.397954465590264E-4	0.0	0.0	22.563021324603746	0.0
28	0.0011096931698385396	0.0	0.0	23.019290166269027	0.0
29	0.0012946420314782962	0.0	0.0	23.463722280789362	0.0
30	0.0012946420314782962	0.0	0.0	24.018198967985352	0.0
31	0.0012946420314782962	0.0	0.0	24.515896354657936	0.0
32	0.0012946420314782962	0.0	0.0	25.005455991418373	0.0
33	0.0012946420314782962	0.0	0.0	25.469492685272524	0.0
34	0.0012946420314782962	0.0	0.0	25.91244520889974	0.0
35	0.0014795908931180528	0.0	0.0	26.39812091956574	0.0
36	0.0016645397547578095	0.0	0.0	26.859568329356932	0.0
37	0.0016645397547578095	0.0	0.0	27.341730011651777	0.0
38	0.0016645397547578095	0.0	0.0	27.835913369953207	0.0
39	0.0016645397547578095	0.0	0.0	28.340083966783183	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	35	1.2103374E-7	45.0	1
TTAGCCG	25	3.888055E-5	45.0	1
TAGAACG	20	7.02997E-4	45.0	1
GCACGCG	25	3.888055E-5	45.0	1
TAACGCG	20	7.02997E-4	45.0	1
AACGGTT	20	7.02997E-4	45.0	22
GTTACGG	25	3.888055E-5	45.0	2
ATGCGAG	20	7.02997E-4	45.0	1
CGTCGAG	25	3.888055E-5	45.0	17
TTACGAG	20	7.02997E-4	45.0	1
CCGCGAA	20	7.02997E-4	45.0	35
TTCGCAA	20	7.02997E-4	45.0	14
AATACGG	40	6.8030204E-9	45.0	2
GCTACGA	60	0.0	44.999996	10
CTAGGCG	30	2.1633605E-6	44.999996	1
CGCGCAA	60	3.6379788E-12	41.249996	18
AACGACG	60	3.6379788E-12	41.249996	13
ACGCGCA	60	3.6379788E-12	41.249996	17
GGCCGAT	55	6.002665E-11	40.909092	8
CGGGAGT	110	0.0	40.909092	6
>>END_MODULE