##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626756
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16025694209549	33.0	31.0	34.0	30.0	34.0
2	32.169740696539	33.0	31.0	34.0	30.0	34.0
3	32.170058204468724	34.0	31.0	34.0	30.0	34.0
4	35.83389389172182	37.0	35.0	37.0	35.0	37.0
5	35.89935955938196	37.0	35.0	37.0	35.0	37.0
6	35.84894440579747	37.0	35.0	37.0	35.0	37.0
7	36.08953723618122	37.0	35.0	37.0	35.0	37.0
8	35.90987561347637	37.0	35.0	37.0	35.0	37.0
9	37.71186713808883	39.0	38.0	39.0	35.0	39.0
10	37.356272616456806	39.0	37.0	39.0	34.0	39.0
11	37.37920179463779	39.0	37.0	39.0	35.0	39.0
12	37.28604432985085	39.0	37.0	39.0	34.0	39.0
13	37.240910338313476	39.0	37.0	39.0	34.0	39.0
14	38.37907734429347	40.0	38.0	41.0	34.0	41.0
15	38.49282974554691	40.0	38.0	41.0	34.0	41.0
16	38.2760404367888	40.0	37.0	41.0	34.0	41.0
17	38.33661903515882	40.0	37.0	41.0	34.0	41.0
18	38.26271467684394	40.0	37.0	41.0	34.0	41.0
19	38.15207832075002	40.0	37.0	41.0	34.0	41.0
20	38.31883699557723	40.0	37.0	41.0	34.0	41.0
21	38.215771368762326	40.0	37.0	41.0	34.0	41.0
22	38.32820268174537	40.0	37.0	41.0	34.0	41.0
23	38.365989316416595	40.0	37.0	41.0	34.0	41.0
24	38.30197876047457	40.0	37.0	41.0	34.0	41.0
25	38.031635915731165	40.0	36.0	41.0	34.0	41.0
26	38.133911761514845	40.0	37.0	41.0	34.0	41.0
27	38.149745674552776	40.0	37.0	41.0	34.0	41.0
28	38.041449942242274	40.0	37.0	41.0	34.0	41.0
29	38.01471226442188	40.0	37.0	41.0	34.0	41.0
30	37.856915609902416	40.0	36.0	41.0	33.0	41.0
31	37.8231895665937	40.0	36.0	41.0	33.0	41.0
32	37.79722092808046	40.0	36.0	41.0	33.0	41.0
33	37.75100517585791	40.0	36.0	41.0	33.0	41.0
34	37.606915609902416	40.0	36.0	41.0	33.0	41.0
35	37.53484769192477	40.0	36.0	41.0	33.0	41.0
36	37.45570525052812	40.0	36.0	41.0	33.0	41.0
37	37.4348709864764	40.0	36.0	41.0	33.0	41.0
38	37.319907587641765	40.0	35.0	41.0	33.0	41.0
39	37.264307003044244	40.0	35.0	41.0	33.0	41.0
40	37.160719642093575	40.0	35.0	41.0	32.0	41.0
41	37.02421995162392	39.0	35.0	41.0	32.0	41.0
42	37.033775504343	39.0	35.0	41.0	32.0	41.0
43	36.97249806942415	39.0	35.0	41.0	32.0	41.0
44	36.878029089470225	39.0	35.0	41.0	32.0	41.0
45	36.85042823682581	39.0	35.0	41.0	32.0	41.0
46	36.70623017569836	39.0	35.0	41.0	31.0	41.0
47	36.65232083936971	39.0	35.0	41.0	31.0	41.0
48	36.57379426762568	39.0	35.0	41.0	31.0	41.0
49	36.52130972818768	39.0	35.0	41.0	31.0	41.0
50	36.40444287729196	39.0	35.0	41.0	31.0	41.0
51	35.33043481035682	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	25.0
10	25.0
11	17.0
12	13.0
13	22.0
14	15.0
15	30.0
16	37.0
17	81.0
18	133.0
19	249.0
20	443.0
21	730.0
22	1191.0
23	1601.0
24	2371.0
25	3261.0
26	4174.0
27	5158.0
28	5845.0
29	6654.0
30	7697.0
31	9963.0
32	13436.0
33	18759.0
34	36154.0
35	48983.0
36	42503.0
37	65795.0
38	119893.0
39	231341.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12674150706176	20.34268519168544	23.073732042453525	12.456841258799276
2	31.957731557416285	25.24874113690176	24.22617414113307	18.567353164548884
3	32.286248556056904	24.91400162104551	25.777176445059958	17.022573377837627
4	29.386874637019826	25.22943537836096	25.471634894600133	19.91205509001908
5	25.41770641206466	29.311247120091394	24.829120104155365	20.44192636368858
6	25.124131240865665	35.02958088953277	24.963143551876648	14.88314431772492
7	78.28038343470186	7.0220309019778036	9.746855235530253	4.950730427790081
8	79.31507636145486	6.536834110882066	9.08726841067337	5.060821116989706
9	73.16355966277148	7.944239863679008	11.375718780514267	7.5164816930352485
10	39.0978307347676	26.429583442360343	19.32538340279152	15.147202420080541
11	30.585905838954876	26.223283063903658	24.74407903554174	18.44673206159973
12	27.918839229301355	23.63280128151944	29.219664430815186	19.22869505836402
13	25.554601790808544	25.478176515262717	29.731506359731696	19.23571533419704
14	20.126971261543567	29.119944603641606	29.48834953315166	21.264734601663168
15	19.20540050673627	25.945184409881993	34.75339685619284	20.09601822718889
16	22.084830460338633	25.83716789308758	31.61980100709048	20.458200639483305
17	22.17513673582702	25.44307513609762	29.013842707528926	23.367945420546434
18	23.757889832725972	25.061267861815445	30.144745323538984	21.0360969819196
19	24.435665554059316	27.208834059825517	27.245690507948865	21.109809878166306
20	26.121169960877914	25.834774617235418	28.324898365552144	19.71915705633452
21	24.22856741698524	27.50990816202797	28.449189158141287	19.812335262845508
22	22.926784905130546	25.518383549579106	28.59214750237732	22.962684042913033
23	22.47844456215816	27.154267370396134	28.60283746785033	21.764450599595378
24	23.120959352602927	24.39258658872033	29.617107773998175	22.869346284678567
25	21.759983151337998	27.110390646439765	27.96526878083337	23.164357421388864
26	21.24718391208062	27.09172309479287	27.92378533272916	23.73730766039735
27	21.32137546349776	27.521555437841837	28.332397296555595	22.824671802104806
28	19.801485745649025	26.839790923421553	30.829062665534913	22.52966066539451
29	21.092418740307235	25.766805583033907	29.719859083917825	23.420916592741033
30	21.941393460932165	25.5857143768867	30.150648737307662	22.322243424873474
31	23.187492421293136	26.588177855497197	27.645208023537073	22.579121699672598
32	23.085538869990874	27.22813981836632	28.148434159385786	21.537887152257017
33	24.156609589696785	26.3933652011309	27.31030895595734	22.139716253214967
34	21.93628780578088	26.03517158192343	29.403946671431946	22.62459394086375
35	21.54969397979437	26.55626751080165	29.01352360408197	22.88051490532201
36	22.82929880208566	27.78274160917486	27.73104685076808	21.656912737971396
37	21.856671495765497	27.99175436693067	29.136537982883294	21.015036154420542
38	21.337649739292484	28.33128043449125	28.154337573154464	22.176732253061797
39	23.262960386498094	25.536253342608607	28.1418925387232	23.058893732170095
40	24.245799003120833	25.127322275335217	29.02820236264192	21.59867635890203
41	20.57547115623943	26.829100957948548	28.09626074580858	24.499167140003447
42	21.57633911761515	26.01411075442437	28.161836504157918	24.247713623802564
43	21.649413806967942	24.841565138586628	28.819030053162635	24.689991001282795
44	21.996598357255458	24.577347484507527	28.89465757009107	24.531396588145945
45	21.87039294398458	25.115515447797865	28.136467780124963	24.87762382809259
46	22.388138286669772	26.871222612946667	27.70312529915948	23.03751380122408
47	20.710132810854624	26.643542303543967	29.693852152990956	22.952472732610456
48	21.148740498694867	25.349099170969247	30.241114564519528	23.261045765816363
49	22.28219594228057	24.36737741641085	30.558303390793228	22.792123250515353
50	20.50159870826925	25.4196210327464	30.074383013485313	24.004397245499046
51	20.79086598293435	24.66542003586723	28.04855478048874	26.495159200709683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	873.0
2	1428.0
3	2117.5
4	2807.0
5	1994.5
6	1182.0
7	1179.0
8	1176.0
9	1190.5
10	1205.0
11	1162.5
12	1120.0
13	1215.0
14	1310.0
15	1353.0
16	1396.0
17	1416.5
18	1437.0
19	1649.5
20	1862.0
21	2509.0
22	3156.0
23	3227.0
24	3298.0
25	3646.0
26	5066.5
27	6139.0
28	7192.5
29	8246.0
30	10144.5
31	12043.0
32	12820.5
33	13598.0
34	16119.0
35	18640.0
36	20389.5
37	22139.0
38	23560.5
39	24982.0
40	27766.5
41	30551.0
42	33866.0
43	37181.0
44	40331.0
45	43481.0
46	49739.5
47	55998.0
48	56379.5
49	56761.0
50	55427.0
51	54093.0
52	48413.5
53	42734.0
54	38836.5
55	34939.0
56	31556.0
57	28173.0
58	27187.5
59	26202.0
60	25269.0
61	24336.0
62	22404.5
63	20473.0
64	18010.5
65	15548.0
66	12637.5
67	9727.0
68	7831.0
69	5935.0
70	4967.0
71	3999.0
72	3171.0
73	2343.0
74	1788.0
75	961.5
76	690.0
77	537.5
78	385.0
79	370.5
80	356.0
81	212.0
82	68.0
83	42.0
84	16.0
85	23.5
86	31.0
87	21.0
88	11.0
89	7.5
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.288008082800857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2910300972672	20.732572517882954
2	9.98155293625549	5.647165002793998
3	3.569014629459265	3.0288094405733457
4	1.803246355537804	2.0404098992293838
5	1.0630663938828049	1.5036015371355376
6	0.7824631671693467	1.3280594638428262
7	0.6061879507857295	1.2003494756066244
8	0.4825027904389684	1.0919234268729205
9	0.4837309938673868	1.2315407637979738
>10	7.47804636971827	50.99901065258099
>50	0.40077151745895306	6.819534987332442
>100	0.052718176978334454	2.637823329138878
>500	0.0022674484721864284	0.4348800528039293
>1k	0.0034011727082796425	1.3043194504082452
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1762	0.2811301367677374	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1467	0.23406237834181087	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	1325	0.21140603360797505	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC	1210	0.19305758540803758	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1209	0.19289803368455985	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG	1161	0.18523955095762945	No Hit
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	957	0.15269099936817518	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.97758617388585E-4	0.0	0.0	0.1605090338185833	0.0
2	7.97758617388585E-4	0.0	0.0	0.8185003414406883	0.0
3	7.97758617388585E-4	0.0	0.0	1.141113926312632	0.0
4	7.97758617388585E-4	0.0	0.0	1.5688720969563914	0.0
5	7.97758617388585E-4	0.0	0.0	2.720835540465508	0.0
6	7.97758617388585E-4	0.0	0.0	3.4332339857935144	0.0
7	7.97758617388585E-4	0.0	0.0	4.082609500347822	0.0
8	7.97758617388585E-4	0.0	0.0	4.998117289662963	0.0
9	7.97758617388585E-4	0.0	0.0	5.3325377020722575	0.0
10	7.97758617388585E-4	0.0	0.0	6.2371959741909135	0.0
11	7.97758617388585E-4	0.0	0.0	7.283855280204737	0.0
12	7.97758617388585E-4	0.0	0.0	8.28089400021699	0.0
13	7.97758617388585E-4	0.0	0.0	8.686793584744303	0.0
14	7.97758617388585E-4	0.0	0.0	8.873947756383664	0.0
15	9.57310340866302E-4	0.0	0.0	9.176298272373938	0.0
16	9.57310340866302E-4	0.0	0.0	9.74717433897721	0.0
17	9.57310340866302E-4	0.0	0.0	10.360650715749031	0.0
18	9.57310340866302E-4	0.0	0.0	11.120116919502964	0.0
19	9.57310340866302E-4	0.0	0.0	11.55505491770322	0.0
20	0.001116862064344019	0.0	0.0	11.977547881472216	0.0
21	0.001116862064344019	0.0	0.0	12.511248396505179	0.0
22	0.001116862064344019	0.0	0.0	13.067126601101545	0.0
23	0.001116862064344019	0.0	0.0	13.58391463344587	0.0
24	0.001116862064344019	0.0	0.0	14.02427739024437	0.0
25	0.001116862064344019	0.0	0.0	14.43655904371079	0.0
26	0.001116862064344019	0.0	0.0	14.811346042159947	0.0
27	0.001116862064344019	0.0	0.0	15.188366764737793	0.0
28	0.0012764137878217361	0.0	0.0	15.577034763129511	0.0
29	0.001435965511299453	0.0	0.0	15.9551723477717	0.0
30	0.001435965511299453	0.0	0.0	16.40654417349016	0.0
31	0.001435965511299453	0.0	0.0	16.817868516615718	0.0
32	0.001435965511299453	0.0	0.0	17.240521032108187	0.0
33	0.001435965511299453	0.0	0.0	17.653121789021565	0.0
34	0.00159551723477717	0.0	0.0	18.04896961496978	0.0
35	0.00159551723477717	0.0	0.0	18.47608957871963	0.0
36	0.0017550689582548872	0.0	0.0	18.872735163285235	0.0
37	0.0017550689582548872	0.0	0.0	19.285495471922086	0.0
38	0.0017550689582548872	0.0	0.0	19.69825578055894	0.0
39	0.0017550689582548872	0.0	0.0	20.144043295955683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.030829E-4	45.0	1
GTCGATT	20	7.030829E-4	45.0	32
CACGAAA	20	7.030829E-4	45.0	25
TTGAGCG	55	1.8189894E-12	45.0	1
AGGTTCG	20	7.030829E-4	45.0	2
AAGCACG	20	7.030829E-4	45.0	1
CCACGAT	20	7.030829E-4	45.0	44
TAAGACG	20	7.030829E-4	45.0	1
TCAACGG	20	7.030829E-4	45.0	2
TTAACGG	30	2.1639153E-6	44.999996	2
CGTTAGG	55	6.002665E-11	40.909092	2
CACGAGG	50	1.0804797E-9	40.500004	2
ACGGGAA	200	0.0	40.500004	5
GCGATAT	50	1.0804797E-9	40.500004	9
CAATAGG	90	0.0	40.0	2
ACGGGAG	260	0.0	39.80769	5
TACGGGA	125	0.0	39.600002	4
TAGTTAC	35	6.243741E-6	38.57143	14
TATCGAG	35	6.243741E-6	38.57143	1
CGTAAGG	100	0.0	38.250004	2
>>END_MODULE