SRR2935594.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311061
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	1498	0.4815775683869081	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	1467	0.4716116774523325	No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	1199	0.3854549429211634	TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	1177	0.37838237516114204	No Hit
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	988	0.3176225884955041	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	834	0.26811461417535465	No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	647	0.2079977882151732	No Hit
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT	628	0.20188966151333662	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC	539	0.173277910120523	No Hit
TCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	502	0.161383137069578	TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	352	0.11316108416034154	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.025345E-4	45.0	41
GAGTGCA	20	7.025345E-4	45.0	9
TCGTTCA	20	7.025345E-4	45.0	16
AGTCCGA	20	7.025345E-4	45.0	11
GTTCTCG	25	3.884225E-5	45.0	42
CGTATGG	20	7.025345E-4	45.0	2
ACGTTAG	20	7.025345E-4	45.0	5
TTTCGCA	35	1.2081182E-7	45.0	14
GACGTAA	20	7.025345E-4	45.0	11
TTCACGG	40	6.7866495E-9	45.0	2
GTTAGTA	25	3.884225E-5	45.0	13
CGCGAGT	20	7.025345E-4	45.0	18
TCGATGG	20	7.025345E-4	45.0	2
ACGGGCC	35	1.2081182E-7	45.0	5
CGTTAGT	20	7.025345E-4	45.0	6
TCTACGC	20	7.025345E-4	45.0	43
CTACGAA	35	1.2081182E-7	45.0	11
TACGAAT	35	1.2081182E-7	45.0	12
TGCGAGA	20	7.025345E-4	45.0	41
GCGGGTT	40	6.7866495E-9	45.0	5