##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935593.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887222
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.114182245255414	33.0	31.0	34.0	30.0	34.0
2	32.13636834974786	33.0	31.0	34.0	30.0	34.0
3	32.13136734661674	33.0	31.0	34.0	30.0	34.0
4	35.79773833381048	37.0	35.0	37.0	35.0	37.0
5	35.86596364833154	37.0	35.0	37.0	35.0	37.0
6	35.81758004197371	37.0	35.0	37.0	35.0	37.0
7	36.080791504268376	37.0	35.0	37.0	35.0	37.0
8	35.882340609227455	37.0	35.0	37.0	35.0	37.0
9	37.69694957969933	39.0	38.0	39.0	35.0	39.0
10	37.34578267896874	39.0	37.0	39.0	34.0	39.0
11	37.34943678132418	39.0	37.0	39.0	34.0	39.0
12	37.259088480673384	39.0	37.0	39.0	34.0	39.0
13	37.227312893503544	39.0	37.0	39.0	34.0	39.0
14	38.37747035127623	40.0	38.0	41.0	34.0	41.0
15	38.484795237268685	40.0	38.0	41.0	34.0	41.0
16	38.294344594701215	40.0	37.0	41.0	34.0	41.0
17	38.32820646918133	40.0	37.0	41.0	34.0	41.0
18	38.24712304248542	40.0	37.0	41.0	34.0	41.0
19	38.0973048459123	40.0	37.0	41.0	34.0	41.0
20	38.20483599369718	40.0	37.0	41.0	34.0	41.0
21	38.13303660188769	40.0	37.0	41.0	34.0	41.0
22	38.235608449745385	40.0	37.0	41.0	34.0	41.0
23	38.265023860995335	40.0	37.0	41.0	34.0	41.0
24	38.195652271922924	40.0	37.0	41.0	34.0	41.0
25	37.968925477501685	40.0	36.0	41.0	34.0	41.0
26	38.03479512455733	40.0	36.0	41.0	34.0	41.0
27	38.024902448316205	40.0	36.0	41.0	34.0	41.0
28	37.91861676108122	40.0	36.0	41.0	34.0	41.0
29	37.9023536386609	40.0	36.0	41.0	34.0	41.0
30	37.710063546665886	40.0	36.0	41.0	33.0	41.0
31	37.672047131383124	40.0	36.0	41.0	33.0	41.0
32	37.60535807272588	40.0	36.0	41.0	33.0	41.0
33	37.503216782270954	40.0	36.0	41.0	33.0	41.0
34	37.42608727015335	40.0	36.0	41.0	33.0	41.0
35	37.317435771430375	40.0	35.0	41.0	33.0	41.0
36	37.19458489532496	40.0	35.0	41.0	32.0	41.0
37	37.15717937562414	40.0	35.0	41.0	32.0	41.0
38	37.04921203486839	40.0	35.0	41.0	32.0	41.0
39	37.00014088920248	40.0	35.0	41.0	32.0	41.0
40	36.891559271523924	39.0	35.0	41.0	31.0	41.0
41	36.76411653453138	39.0	35.0	41.0	31.0	41.0
42	36.751962868368906	39.0	35.0	41.0	31.0	41.0
43	36.689307749356985	39.0	35.0	41.0	31.0	41.0
44	36.568693066673276	39.0	35.0	41.0	31.0	41.0
45	36.54271760619101	39.0	35.0	41.0	31.0	41.0
46	36.404503044333886	39.0	35.0	41.0	31.0	41.0
47	36.32458618023448	39.0	35.0	41.0	30.0	41.0
48	36.22525027557928	39.0	35.0	41.0	30.0	41.0
49	36.173217075320494	39.0	35.0	41.0	30.0	41.0
50	36.04936194097982	38.0	35.0	40.0	30.0	41.0
51	34.89176891465721	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	26.0
11	26.0
12	29.0
13	17.0
14	28.0
15	54.0
16	72.0
17	123.0
18	244.0
19	381.0
20	706.0
21	1230.0
22	1861.0
23	2791.0
24	3860.0
25	5394.0
26	7365.0
27	8622.0
28	9307.0
29	10316.0
30	12085.0
31	15113.0
32	19746.0
33	27221.0
34	51645.0
35	69650.0
36	61588.0
37	93672.0
38	169577.0
39	314251.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78207709006314	20.12089420686142	23.68516560680416	13.411863096271281
2	32.362475231678204	25.760294492246587	24.239480085029452	17.637750191045757
3	31.285292745220474	24.2703629982124	27.506418912064852	16.93792534450228
4	28.92286259808706	23.925353519186853	28.21199203806939	18.939791844656693
5	26.031252606450245	29.031516351037283	25.401308804335333	19.535922238177143
6	25.152780251166	34.90963930109939	25.222886718318527	14.714693729416087
7	77.50326299392937	5.950596355816244	11.557648480312706	4.988492169941683
8	78.01643782503139	7.185236614962208	9.766777649787764	5.031547910218637
9	71.95110130271793	7.628304978911704	12.055607277547221	8.364986440823154
10	40.16751162617699	24.42049453237183	19.52938497918221	15.88260886226897
11	30.550978221910636	25.311027003388105	25.18264876209111	18.955346012610146
12	28.272405328091505	21.841658570233832	29.622800155992522	20.26313594568214
13	24.943024406518326	23.021971952904686	31.507897685134047	20.527105955442945
14	20.15797624495335	27.214045639084695	30.009400127589263	22.618577988372696
15	18.968307819238024	25.587620685690844	34.79974572316737	20.644325771903763
16	22.012867129083816	24.279605329894885	31.56154829343727	22.145979247584034
17	21.191990279772142	25.38823428634547	29.540746284469954	23.879029149412435
18	22.720919905051947	25.584352056193378	29.32400233537942	22.370725703375253
19	23.710638374611992	27.08499112961581	27.381083877541357	21.823286618230835
20	25.06779588423191	25.897126085692197	28.983501310833137	20.05157671924276
21	24.726731302875717	26.951090031581725	28.3054297571521	20.016748908390458
22	22.704125912116695	24.841809603458888	28.567934519207146	23.886129965217272
23	22.307945474751527	26.240219471564053	28.832355374415876	22.619479679268547
24	22.816273717288347	24.928033795374777	29.049662880316312	23.206029607020564
25	22.41535940271995	26.228272067194002	27.830689500485782	23.52567902960026
26	21.67833980672256	26.481759920290525	28.36494135627836	23.47495891670856
27	22.829235523916225	26.84299983544141	28.410138612432966	21.9176260282094
28	20.528007646338796	26.927984202375505	30.553683294598194	21.990324856687504
29	23.34973659354705	24.97672510375081	29.366832652932413	22.306705649769732
30	23.161170484951906	25.067232327421994	29.771353731084215	22.00024345654188
31	23.138853635279556	25.77010038073898	27.894033285919424	23.197012698062043
32	24.102535780221864	25.854408479501185	28.617978363926955	21.42507737635
33	23.704439249703004	25.255573013293176	27.85266821607219	23.187319520931627
34	22.276611716120655	24.882047559686303	29.391516441206374	23.44982428298667
35	22.5042886673234	23.54686876565279	30.3092123504602	23.63963021656361
36	23.207607566088306	25.922148008052098	28.775548847977166	22.094695577882423
37	21.37886571793756	26.844803217233114	30.075561697072434	21.700769367756887
38	21.709560853991448	25.598440976441072	29.361084373471353	23.330913796096127
39	22.880857327703776	24.419141996028053	28.286945093787125	24.413055582481046
40	23.403725335936215	23.37148988640949	30.654898097657636	22.569886679996664
41	20.30664253140702	24.759530309212348	29.577602899837924	25.35622425954271
42	21.154006550784356	24.987319971777076	30.349901152135544	23.508772325303024
43	22.403524709711885	23.95240424606243	29.19630036225432	24.447770681971367
44	22.666142183128912	24.39445820775409	28.991841951619772	23.94755765749722
45	22.329586056251987	23.404288892746123	28.975386092770467	25.290738958231422
46	22.951640063028194	25.388008863621508	28.031315724812956	23.629035348537343
47	21.106780490114087	23.879029149412435	31.70356460953403	23.310625750939447
48	21.37672420205991	23.462447955528607	30.86634461273503	24.29448322967645
49	21.414482508323733	23.491527486919846	31.152518760806203	23.941471243950218
50	20.638915626528647	23.18822121182748	30.870627644490327	25.302235517153544
51	20.41450730482337	23.073593756692237	29.1646284695375	27.347270468946896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	993.0
2	1661.0
3	4197.0
4	6733.0
5	4526.5
6	2320.0
7	2264.0
8	2208.0
9	2166.5
10	2125.0
11	2180.5
12	2236.0
13	2129.5
14	2023.0
15	2169.0
16	2315.0
17	2452.5
18	2590.0
19	2585.5
20	2581.0
21	2920.0
22	3259.0
23	3426.5
24	3594.0
25	4524.0
26	6129.0
27	6804.0
28	9002.0
29	11200.0
30	12946.0
31	14692.0
32	16073.5
33	17455.0
34	20216.5
35	22978.0
36	25047.5
37	27117.0
38	29433.0
39	31749.0
40	35824.0
41	39899.0
42	43098.5
43	46298.0
44	51977.5
45	57657.0
46	71663.5
47	85670.0
48	85309.5
49	84949.0
50	83254.5
51	81560.0
52	70935.5
53	60311.0
54	53357.0
55	46403.0
56	43674.0
57	40945.0
58	40690.0
59	40435.0
60	39398.5
61	38362.0
62	34368.0
63	30374.0
64	26761.0
65	23148.0
66	18771.0
67	14394.0
68	12268.0
69	10142.0
70	8402.5
71	6663.0
72	5190.0
73	3717.0
74	2944.0
75	1823.0
76	1475.0
77	1046.0
78	617.0
79	474.5
80	332.0
81	232.0
82	132.0
83	106.0
84	80.0
85	54.5
86	29.0
87	18.5
88	8.0
89	11.0
90	14.0
91	13.0
92	12.0
93	7.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	887222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.560861285217953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21038832639923	21.195126069124456
2	9.64057202380744	5.506860805642344
3	3.380118798894639	2.896173124283619
4	1.7561419686630302	2.006277086565375
5	1.0686476744340752	1.5260748996141185
6	0.7788566150729705	1.3346889446504102
7	0.620466916820902	1.2404748680372053
8	0.5163851293215116	1.179872323864082
9	0.44928235639308206	1.1548701952954803
>10	7.1170168309412345	48.533561422538824
>50	0.40301917055812914	6.969448997956475
>100	0.049187378510254114	2.5805892299627167
>500	0.006346758517452142	1.2259441591635156
>1k	0.0035700516660668296	2.650037873301339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3957	0.4459988593610168	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	3922	0.44205396169166233	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	3911	0.4408141367098652	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	3908	0.4404760026239205	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	2191	0.246950594101589	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1755	0.19780844027763064	Illumina PCR Primer Index 5 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1333	0.15024424552141402	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1232	0.1388603979612769	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1182	0.13322482986219908	Illumina PCR Primer Index 5 (95% over 22bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	1000	0.1127113619815559	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	920	0.10369445302303144	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5084544792622363E-4	0.0	0.0	0.14990611143546936	0.0
2	4.5084544792622363E-4	0.0	0.0	0.7586601774978529	0.0
3	4.5084544792622363E-4	0.0	0.0	1.1577711102745423	0.0
4	4.5084544792622363E-4	0.0	0.0	1.6396121827456938	0.0
5	4.5084544792622363E-4	0.0	0.0	3.0480533620672166	0.0
6	4.5084544792622363E-4	0.0	0.0	3.9533510215030736	0.0
7	4.5084544792622363E-4	0.0	0.0	4.742443266735947	0.0
8	4.5084544792622363E-4	0.0	0.0	5.805085987498056	0.0
9	4.5084544792622363E-4	0.0	0.0	6.290533823552617	0.0
10	4.5084544792622363E-4	0.0	0.0	7.649156580878292	0.0
11	4.5084544792622363E-4	0.0	0.0	8.875568910599602	0.0
12	4.5084544792622363E-4	0.0	0.0	10.2010545275027	0.0
13	4.5084544792622363E-4	0.0	0.0	10.590246860425012	0.0
14	4.5084544792622363E-4	0.0	0.0	10.759877460207253	0.0
15	4.5084544792622363E-4	0.0	0.0	11.094404782568512	0.0
16	4.5084544792622363E-4	0.0	0.0	11.644999785848412	0.0
17	4.5084544792622363E-4	0.0	0.0	12.289483353658948	0.0
18	4.5084544792622363E-4	0.0	0.0	13.002833563640216	0.0
19	5.635568099077795E-4	0.0	0.0	13.503384722200305	0.0
20	5.635568099077795E-4	0.0	0.0	13.945213261168005	0.0
21	5.635568099077795E-4	0.0	0.0	14.47788715789284	0.0
22	6.762681718893355E-4	0.0	0.0	15.058350672097852	0.0
23	6.762681718893355E-4	0.0	0.0	15.619991388851945	0.0
24	6.762681718893355E-4	0.0	0.0	16.06159450509568	0.0
25	6.762681718893355E-4	0.0	0.0	16.487868876109925	0.0
26	6.762681718893355E-4	0.0	0.0	16.863648556956434	0.0
27	6.762681718893355E-4	0.0	0.0	17.273805203207313	0.0
28	6.762681718893355E-4	0.0	0.0	17.666942433798983	0.0
29	6.762681718893355E-4	0.0	0.0	18.078789750479586	0.0
30	7.889795338708914E-4	0.0	0.0	18.579340909039676	0.0
31	7.889795338708914E-4	0.0	0.0	19.016210148080187	0.0
32	7.889795338708914E-4	0.0	0.0	19.43572183737554	0.0
33	7.889795338708914E-4	0.0	0.0	19.851175917639555	0.0
34	9.016908958524473E-4	0.0	0.0	20.24791991181463	0.0
35	9.016908958524473E-4	0.0	0.0	20.665628219318275	0.0
36	9.016908958524473E-4	0.0	0.0	21.06372474983713	0.0
37	9.016908958524473E-4	0.0	0.0	21.462610259889857	0.0
38	9.016908958524473E-4	0.0	0.0	21.863862708544197	0.0
39	0.0010144022578340032	0.0	0.0	22.269398188953836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	35	1.2115015E-7	45.0	1
ACGATCC	20	7.032416E-4	45.0	44
TGCGACG	35	1.2115015E-7	45.0	1
TCTCGAC	20	7.032416E-4	45.0	14
TACACCG	20	7.032416E-4	45.0	18
TCACGAC	80	0.0	42.1875	25
TAGTACG	60	3.6379788E-12	41.250004	1
CGGGTAC	110	0.0	40.909092	6
AGTACGG	125	0.0	39.6	2
GTTCACG	40	3.4570257E-7	39.375	1
TACGAAT	185	0.0	38.91892	12
CGTTTTT	2035	0.0	38.918915	1
ACGGGTA	145	0.0	38.793106	5
ACGCTAG	35	6.246679E-6	38.571426	1
TCGAGAC	35	6.246679E-6	38.571426	44
CGAATAT	190	0.0	37.894737	14
CACGACC	60	1.546141E-10	37.500004	27
ACGTGCG	30	1.139712E-4	37.500004	1
AATCGCG	30	1.139712E-4	37.500004	1
AAATGCG	30	1.139712E-4	37.500004	1
>>END_MODULE