##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935592.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503948
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13576400739759	33.0	31.0	34.0	30.0	34.0
2	32.14338185685825	33.0	31.0	34.0	30.0	34.0
3	32.15390476795225	33.0	31.0	34.0	30.0	34.0
4	35.806259772833705	37.0	35.0	37.0	35.0	37.0
5	35.866498130759524	37.0	35.0	37.0	35.0	37.0
6	35.795107828585486	37.0	35.0	37.0	35.0	37.0
7	36.04326041575718	37.0	35.0	37.0	35.0	37.0
8	35.816955320787066	37.0	35.0	37.0	35.0	37.0
9	37.6320612444141	39.0	37.0	39.0	35.0	39.0
10	37.31931865986173	39.0	37.0	39.0	34.0	39.0
11	37.33558224261233	39.0	37.0	39.0	34.0	39.0
12	37.27213323596879	39.0	37.0	39.0	34.0	39.0
13	37.227789375094254	39.0	37.0	39.0	34.0	39.0
14	38.41319540905014	40.0	38.0	41.0	34.0	41.0
15	38.497833109765295	40.0	38.0	41.0	34.0	41.0
16	38.31104399660283	40.0	37.0	41.0	34.0	41.0
17	38.33253430909538	40.0	38.0	41.0	34.0	41.0
18	38.253530126124126	40.0	37.0	41.0	34.0	41.0
19	38.130983752291904	40.0	37.0	41.0	34.0	41.0
20	38.2446026177304	40.0	37.0	41.0	34.0	41.0
21	38.19869708779477	40.0	37.0	41.0	34.0	41.0
22	38.294139474707706	40.0	37.0	41.0	34.0	41.0
23	38.32378142189273	40.0	37.0	41.0	34.0	41.0
24	38.22924389024264	40.0	37.0	41.0	34.0	41.0
25	37.982776000698486	40.0	37.0	41.0	34.0	41.0
26	38.06768753919055	40.0	37.0	41.0	34.0	41.0
27	38.06358195686857	40.0	37.0	41.0	34.0	41.0
28	37.96974092565106	40.0	37.0	41.0	34.0	41.0
29	37.94981029788788	40.0	37.0	41.0	34.0	41.0
30	37.81076817449419	40.0	36.0	41.0	33.0	41.0
31	37.78438846865153	40.0	36.0	41.0	33.0	41.0
32	37.70733885242128	40.0	36.0	41.0	33.0	41.0
33	37.64380451951392	40.0	36.0	41.0	33.0	41.0
34	37.58635811631359	40.0	36.0	41.0	33.0	41.0
35	37.50776072134427	40.0	36.0	41.0	33.0	41.0
36	37.40645860287172	40.0	36.0	41.0	33.0	41.0
37	37.36851024312032	40.0	36.0	41.0	33.0	41.0
38	37.231450467111685	40.0	35.0	41.0	32.0	41.0
39	37.1616873169454	40.0	35.0	41.0	32.0	41.0
40	37.03233468532468	40.0	35.0	41.0	31.0	41.0
41	36.90737536412487	39.0	35.0	41.0	31.0	41.0
42	36.93510838419837	39.0	35.0	41.0	31.0	41.0
43	36.89658853691254	39.0	35.0	41.0	31.0	41.0
44	36.817955423972315	39.0	35.0	41.0	31.0	41.0
45	36.78836110074849	39.0	35.0	41.0	31.0	41.0
46	36.66750736187067	39.0	35.0	41.0	31.0	41.0
47	36.55973235333804	39.0	35.0	41.0	31.0	41.0
48	36.46104359973648	39.0	35.0	41.0	31.0	41.0
49	36.40440481954487	39.0	35.0	41.0	31.0	41.0
50	36.3017970108027	39.0	35.0	41.0	31.0	41.0
51	35.11377165898069	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	16.0
10	19.0
11	21.0
12	10.0
13	13.0
14	19.0
15	31.0
16	54.0
17	62.0
18	116.0
19	211.0
20	423.0
21	755.0
22	1059.0
23	1503.0
24	2082.0
25	2773.0
26	3570.0
27	4247.0
28	4756.0
29	5422.0
30	6781.0
31	8571.0
32	11230.0
33	15771.0
34	28246.0
35	37720.0
36	34782.0
37	52978.0
38	96479.0
39	184104.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6678069959599	19.4160905490249	22.617214474509275	12.298887980505926
2	32.1473644106138	26.808123060315747	23.926476541230443	17.118035987840017
3	31.38220610062943	26.15468262598522	25.75583195091557	16.707279322469777
4	28.816465190853023	23.88817893909689	28.20628318794796	19.08907268210212
5	27.4958924333463	28.39360410201052	23.869129354615954	20.241374110027223
6	25.583790391072093	35.15779405811711	24.183249065379762	15.075166485431035
7	77.01330295982919	6.380221768912666	11.541071697873589	5.065403573384556
8	77.21233143102066	8.249462246104757	9.291236397406081	5.2469699254685
9	70.7789295720987	8.140324001682714	11.4289569558764	9.651789470342178
10	39.13241048679625	26.460865009881974	18.816227071047013	15.590497432274757
11	31.129402239913638	26.08007175343488	24.3227475850683	18.467778421583176
12	27.98681610007382	22.703334471016852	28.770230261852415	20.53961916705692
13	25.213910959067203	24.121536348988386	30.265027344091056	20.399525347853352
14	20.44476811099558	28.228706136347405	28.917666108408007	22.40885964424901
15	19.144038670656496	26.366807686507336	33.516553295181254	20.972600347654915
16	21.824077087318532	25.07322184034861	30.848420868819797	22.254280203513062
17	21.36629176026098	25.667926055862907	29.08554057164628	23.88024161222983
18	22.251700572281266	26.109241429671314	28.18207434100344	23.45698365704398
19	23.39130227721908	27.414733266130632	26.67279163723241	22.521172819417874
20	26.502932842277378	25.39825537555462	28.55770833498694	19.541103447181058
21	24.783310976529325	27.291307833347883	27.6576154682626	20.267765721860194
22	22.374530705548985	25.454411963139055	28.062816004825898	24.108241326486066
23	22.901370776349943	27.396278981164723	28.049322549151896	21.653027693333442
24	23.551636279933643	25.188114646749266	28.612674323541317	22.64757474977577
25	22.040369244445856	28.090795082032272	26.49499551541032	23.373840158111552
26	21.471461341249494	27.187924150904458	27.61356330415043	23.72705120369562
27	22.515021391095907	27.52149031249256	27.05695825759801	22.90653003881353
28	20.841039154833435	27.791557859144195	28.98136315651615	22.386039829506217
29	22.02112122679324	25.965178947034218	28.85416749347155	23.159532332700994
30	21.566709263654186	26.431100034130505	28.824997817235108	23.177192884980197
31	24.11796455189821	26.31878685896164	26.289617182725202	23.273631406414946
32	24.14197496567106	28.176518212196495	26.354107963520047	21.327398858612394
33	22.428702961416654	26.55015993713637	27.22304682229119	23.798090279155787
34	22.21360140331939	25.80841674140983	30.013017216061975	21.964964639208805
35	22.97240985181011	24.830934937731673	27.93581877495297	24.26083643550525
36	22.445768214180827	26.70255661298388	27.652654638970688	23.199020533864605
37	22.40092231738195	27.684999245953946	28.061228539452483	21.85284989721162
38	22.63646249216189	26.75276020541802	26.493011183693554	24.117766118726536
39	22.576932540658955	26.13146594489908	28.6027526649575	22.688848849484472
40	23.641129640359722	23.958821148213705	31.452054577059542	20.94799463436704
41	20.816433441545556	25.629430020557674	29.62508036543453	23.92905617246224
42	22.210624905744243	25.790756189130626	28.58509211267829	23.41352679244684
43	22.98391897576734	25.648281171866937	26.971830426948813	24.395969425416908
44	22.05961726209847	25.47346154761999	27.525657409097764	24.94126378118377
45	21.829434782953797	24.88510719359934	27.792351591830904	25.49310643161596
46	23.093255653361062	26.824394580393214	26.62794574043354	23.454404025812188
47	21.528808527863987	25.452030765078938	30.470604109947853	22.548556597109226
48	21.800463539889037	24.750966369546067	28.954971544683183	24.493598545881717
49	21.267472040766112	25.0581409193012	30.204505226729744	23.46988181320295
50	20.640026351925197	24.758903696413125	29.926500353211043	24.67456959845063
51	20.14453872224912	24.07569828633113	28.15885765991729	27.620905331502456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	524.0
2	829.0
3	1814.0
4	2799.0
5	1885.5
6	972.0
7	1002.5
8	1033.0
9	1039.0
10	1045.0
11	1055.0
12	1065.0
13	1054.5
14	1044.0
15	1051.5
16	1059.0
17	1056.0
18	1053.0
19	1171.5
20	1290.0
21	1451.5
22	1613.0
23	1981.5
24	2350.0
25	2485.0
26	3616.0
27	4612.0
28	5545.5
29	6479.0
30	7647.0
31	8815.0
32	9928.5
33	11042.0
34	12345.0
35	13648.0
36	14934.0
37	16220.0
38	17724.0
39	19228.0
40	21883.5
41	24539.0
42	25581.0
43	26623.0
44	29250.5
45	31878.0
46	41853.5
47	51829.0
48	48441.0
49	45053.0
50	43534.5
51	42016.0
52	37405.0
53	32794.0
54	29683.0
55	26572.0
56	25526.0
57	24480.0
58	24166.5
59	23853.0
60	23133.0
61	22413.0
62	20536.0
63	18659.0
64	15453.0
65	12247.0
66	10314.5
67	8382.0
68	6776.5
69	5171.0
70	4305.0
71	3439.0
72	2867.5
73	2296.0
74	1839.5
75	1025.5
76	668.0
77	483.5
78	299.0
79	251.0
80	203.0
81	132.5
82	62.0
83	44.0
84	26.0
85	17.0
86	8.0
87	9.0
88	10.0
89	6.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	503948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.402714572045753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65380082600015	22.088727690871448
2	8.375801680212227	5.0929421559110715
3	3.0490032126443847	2.7809392320983326
4	1.7722984533086128	2.1553073604967965
5	1.33306145982591	2.026434353504089
6	1.1342762843833973	2.069104687196942
7	0.951721092541738	2.0254433310119406
8	0.8651837051437263	2.1043146591895763
9	0.8375407189363798	2.291716027825988
>10	8.904096100542983	50.119526043099114
>50	0.07733799490605738	1.5879144104321803
>100	0.0393244041895207	2.233919996907413
>500	0.003277033682460058	0.7563964238926536
>1k	0.003277033682460058	2.66731362756245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	4178	0.829053791264178	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	3203	0.6355814488796463	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	2894	0.5742655988316254	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.3174930746823085	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1511	0.2998325224031051	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	979	0.1942660750712375	TruSeq Adapter, Index 27 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	796	0.15795280465444847	No Hit
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	793	0.15735750513941915	Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT	634	0.12580663084286473	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT	594	0.11786930397580703	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.937326867057712E-4	0.0	0.0	0.16727916372324128	0.0
2	7.937326867057712E-4	0.0	0.0	0.8064324096930636	0.0
3	7.937326867057712E-4	0.0	0.0	1.1120194940747854	0.0
4	7.937326867057712E-4	0.0	0.0	1.6237786438283315	0.0
5	7.937326867057712E-4	0.0	0.0	3.3644344257740877	0.0
6	7.937326867057712E-4	0.0	0.0	4.151221951471183	0.0
7	7.937326867057712E-4	0.0	0.0	4.827283767372824	0.0
8	7.937326867057712E-4	0.0	0.0	5.666854516735854	0.0
9	7.937326867057712E-4	0.0	0.0	5.990499019740132	0.0
10	7.937326867057712E-4	0.0	0.0	7.219594085104019	0.0
11	7.937326867057712E-4	0.0	0.0	8.145681697317977	0.0
12	7.937326867057712E-4	0.0	0.0	9.519831411177345	0.0
13	7.937326867057712E-4	0.0	0.0	9.915308722328493	0.0
14	7.937326867057712E-4	0.0	0.0	10.088540881202029	0.0
15	7.937326867057712E-4	0.0	0.0	10.436195797979156	0.0
16	7.937326867057712E-4	0.0	0.0	10.960257804376642	0.0
17	7.937326867057712E-4	0.0	0.0	11.530356306603062	0.0
18	7.937326867057712E-4	0.0	0.0	12.129227618722567	0.0
19	7.937326867057712E-4	0.0	0.0	12.609039027836205	0.0
20	7.937326867057712E-4	0.0	0.0	12.997968044322032	0.0
21	7.937326867057712E-4	0.0	0.0	13.458531435783057	0.0
22	7.937326867057712E-4	0.0	0.0	13.995690031511188	0.0
23	7.937326867057712E-4	0.0	0.0	14.464190749839268	0.0
24	7.937326867057712E-4	0.0	0.0	14.868795986887536	0.0
25	7.937326867057712E-4	0.0	0.0	15.226570995420163	0.0
26	9.92165858382214E-4	0.0	0.0	15.574424345368966	0.0
27	9.92165858382214E-4	0.0	0.0	15.94966147300912	0.0
28	9.92165858382214E-4	0.0	0.0	16.291958694150985	0.0
29	9.92165858382214E-4	0.0	0.0	16.655488264662228	0.0
30	9.92165858382214E-4	0.0	0.0	17.086286680371785	0.0
31	9.92165858382214E-4	0.0	0.0	17.45199901577147	0.0
32	9.92165858382214E-4	0.0	0.0	17.86077134942494	0.0
33	9.92165858382214E-4	0.0	0.0	18.237000642923476	0.0
34	9.92165858382214E-4	0.0	0.0	18.59259288656766	0.0
35	0.0011905990300586568	0.0	0.0	18.94739139752514	0.0
36	0.0013890322017350997	0.0	0.0	19.319255161246794	0.0
37	0.0013890322017350997	0.0	0.0	19.68199099907133	0.0
38	0.0017858985450879853	0.0	0.0	20.03143181439355	0.0
39	0.0017858985450879853	0.0	0.0	20.399922214196703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTA	25	3.887676E-5	45.000004	42
TAGCGCG	25	3.887676E-5	45.000004	1
CGCCCTA	25	3.887676E-5	45.000004	21
CTACGTT	20	7.0295116E-4	45.0	27
AATTGCG	35	1.2101191E-7	45.0	1
CGCAGTA	20	7.0295116E-4	45.0	37
CGTATGG	85	0.0	42.35294	2
GCACGCG	55	6.002665E-11	40.909092	1
GCTACGA	45	1.9246727E-8	40.0	10
TACGGCT	445	0.0	39.943817	7
TCGTTGA	85	0.0	39.705883	24
CGTTGAT	85	0.0	39.705883	25
ATTGCGG	105	0.0	38.57143	2
TAGCGAG	35	6.241309E-6	38.571426	1
TACGGGT	70	0.0	38.571426	4
CGAAAGG	65	9.094947E-12	38.07692	2
CGTTTTT	880	0.0	37.840908	1
GGCGATA	150	0.0	37.5	8
ACACGGT	30	1.1390123E-4	37.499996	36
GCGAAGT	30	1.1390123E-4	37.499996	9
>>END_MODULE