##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935591.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664234
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.103480098880816	33.0	31.0	34.0	30.0	34.0
2	32.09225062252158	33.0	31.0	34.0	30.0	34.0
3	32.0905509203082	33.0	31.0	34.0	30.0	34.0
4	35.75066618089408	37.0	35.0	37.0	33.0	37.0
5	35.82976481179825	37.0	35.0	37.0	35.0	37.0
6	35.7829560064676	37.0	35.0	37.0	35.0	37.0
7	36.022281304480046	37.0	35.0	37.0	35.0	37.0
8	35.86259661504831	37.0	35.0	37.0	35.0	37.0
9	37.644495765046656	39.0	38.0	39.0	35.0	39.0
10	37.291359671441086	39.0	37.0	39.0	34.0	39.0
11	37.318490170632636	39.0	37.0	39.0	34.0	39.0
12	37.19320600872584	39.0	37.0	39.0	34.0	39.0
13	37.13354028851278	39.0	37.0	39.0	33.0	39.0
14	38.14198159082492	40.0	37.0	41.0	33.0	41.0
15	38.31224237241695	40.0	38.0	41.0	33.0	41.0
16	38.12245835052105	40.0	37.0	41.0	33.0	41.0
17	38.20096532246166	40.0	37.0	41.0	34.0	41.0
18	38.10803120587022	40.0	37.0	41.0	34.0	41.0
19	38.02322073245272	40.0	37.0	41.0	34.0	41.0
20	38.1764031952595	40.0	37.0	41.0	34.0	41.0
21	38.067608704161486	40.0	37.0	41.0	34.0	41.0
22	38.17593498676671	40.0	37.0	41.0	34.0	41.0
23	38.233944664079225	40.0	37.0	41.0	34.0	41.0
24	38.17617285474697	40.0	37.0	41.0	34.0	41.0
25	37.88300056907656	40.0	36.0	41.0	33.0	41.0
26	38.012477530508825	40.0	36.0	41.0	34.0	41.0
27	37.99787123212603	40.0	36.0	41.0	34.0	41.0
28	37.76547421541204	40.0	36.0	41.0	33.0	41.0
29	37.62722022660689	40.0	36.0	41.0	33.0	41.0
30	37.46224372736114	40.0	36.0	41.0	33.0	41.0
31	37.457332807414254	40.0	36.0	41.0	33.0	41.0
32	37.48345010944938	40.0	35.0	41.0	33.0	41.0
33	37.42491802587642	40.0	35.0	41.0	33.0	41.0
34	37.30117097288004	40.0	35.0	41.0	32.0	41.0
35	37.23294200537762	40.0	35.0	41.0	32.0	41.0
36	37.12012182453774	40.0	35.0	41.0	32.0	41.0
37	37.085307286287666	40.0	35.0	41.0	32.0	41.0
38	36.91872743641548	39.0	35.0	41.0	31.0	41.0
39	36.83949782757281	39.0	35.0	41.0	31.0	41.0
40	36.746870831664744	39.0	35.0	41.0	31.0	41.0
41	36.58023527853136	39.0	35.0	41.0	31.0	41.0
42	36.593438456929334	39.0	35.0	41.0	31.0	41.0
43	36.48999750088071	39.0	35.0	41.0	30.0	41.0
44	36.403281072634044	39.0	35.0	41.0	30.0	41.0
45	36.378798736589815	39.0	35.0	41.0	30.0	41.0
46	36.21424528103048	39.0	35.0	41.0	30.0	41.0
47	36.19666864388152	38.0	35.0	41.0	30.0	41.0
48	36.11352475181939	38.0	35.0	41.0	30.0	41.0
49	35.9711411942177	38.0	35.0	40.0	30.0	41.0
50	35.81656765537446	38.0	35.0	40.0	29.0	41.0
51	34.64103312989097	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	14.0
10	21.0
11	15.0
12	14.0
13	14.0
14	22.0
15	24.0
16	71.0
17	101.0
18	180.0
19	319.0
20	569.0
21	985.0
22	1460.0
23	2167.0
24	2969.0
25	4141.0
26	5393.0
27	6498.0
28	7133.0
29	8360.0
30	10116.0
31	12461.0
32	16044.0
33	22479.0
34	42115.0
35	50615.0
36	47493.0
37	71384.0
38	128400.0
39	222504.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46505297831788	19.882300514577693	22.886663434873856	12.765983072230568
2	32.897292219308255	23.28878076099688	24.821523740127727	18.992403279567142
3	32.75396923373389	23.808326583703934	26.27297006777732	17.16473411478485
4	28.42462144364787	25.11584772836079	26.121216318345642	20.3383145096457
5	24.589527184696962	28.871000280021796	25.31562672190824	21.223845813373
6	25.067973033599607	33.97070911757002	25.988431787592926	14.972886061237457
7	79.28591430128539	6.257734473092313	9.420475314422328	5.035875911199969
8	79.59137894175848	5.576197544841125	9.282722654967978	5.54970085843242
9	73.18023467633394	8.113255268474665	11.819328730537732	6.887181324653661
10	39.756621913361855	26.788149959201125	18.2413125494931	15.213915577943919
11	29.55313940569137	27.077806917441745	24.687384265183653	18.68166941168323
12	27.22037715624313	22.809130517257472	31.086484582240598	18.884007744258803
13	26.28185247969842	24.002836349840567	30.842293529087637	18.873017641373373
14	20.481938593929247	27.45387920521985	31.721802858631143	20.34237934221976
15	18.96154066187518	25.329778361240162	35.51233450862196	20.19634646826269
16	23.421264193040404	26.161412996022488	29.868690852922313	20.548631958014795
17	23.952853964115057	23.856351827819715	27.596148345312045	24.594645862753186
18	26.29630521773953	23.11956328643219	29.941105092482466	20.64302640334581
19	25.194434491459333	25.39722447209869	27.091055260646097	22.317285775795877
20	24.907487421601424	26.243161295567525	27.166932135361936	21.682419147469115
21	24.837030323651003	26.75156646603456	29.287419794831337	19.1239834154831
22	22.741383307689762	24.859462177485643	28.991891411761518	23.407263103063077
23	21.634695002062525	25.182992740510123	28.990837566279353	24.191474691148
24	21.860067385891117	24.893034683560312	29.18865941821707	24.0582385123315
25	22.576531764408326	26.638804999442968	26.633987420095927	24.15067581605278
26	20.833019688844594	25.61687598045267	27.973123929217714	25.576980401485017
27	21.372287477003585	24.76326113989949	29.022302381389693	24.84214900170723
28	20.523791314506635	25.990388929202663	29.291635176759996	24.19418457953071
29	21.0135283650039	25.48996287452916	30.117699485422307	23.378809275044638
30	23.474257565857815	25.08498511066883	28.364100603100717	23.07665672037264
31	22.678152578759896	28.614012531728278	26.15117563991003	22.556659249601797
32	22.389398916646844	27.884902007425094	26.089902052589903	23.635797023338164
33	22.63599875947332	24.965147824411275	27.244766151687504	25.1540872644279
34	20.757142814128756	26.8333147655796	28.539942249267575	23.869600171024068
35	20.011622410174727	28.105155713197455	27.58380329823526	24.299418578392554
36	21.893639891965783	27.75407461828211	26.559164390862257	23.79312109888985
37	20.723118659990305	29.341165914421723	26.093816335809368	23.8418990897786
38	21.057488776545615	28.30930063802817	25.109073007404014	25.524137578022206
39	22.02702662013688	25.329928910594763	25.845259351373162	26.797785117895202
40	23.4658268020005	25.08031808067639	27.68090763194898	23.77294748537413
41	19.79287419794831	25.863626372633743	26.815098293673618	27.52840113574433
42	21.129601917396577	25.83110771204124	27.930217363158167	25.109073007404014
43	21.171304088619372	24.978095068906438	28.017535988823216	25.83306485365097
44	21.325617177079163	24.827997362375307	27.038513535892474	26.80787192465306
45	20.834976830454327	24.30604274999473	26.78649391630058	28.07248650325036
46	21.28060292005528	25.71307701803882	27.25440131038158	25.75191875152431
47	19.11269221388848	26.22223493527883	28.750861895055056	25.914210955777634
48	19.635249023687436	26.07063173520175	27.863523999072616	26.430595242038198
49	22.388194521810085	24.248382347184876	28.72993553476636	24.633487596238677
50	19.98346968086548	24.639961218486256	29.39792302110401	25.97864607954426
51	20.583408858926223	24.434762448173387	27.44213635556144	27.53969233733895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	314.0
1	775.5
2	1237.0
3	2107.0
4	2977.0
5	2047.5
6	1118.0
7	1124.0
8	1130.0
9	1150.5
10	1171.0
11	1215.5
12	1260.0
13	1315.0
14	1370.0
15	1374.0
16	1378.0
17	1326.5
18	1275.0
19	1488.0
20	1701.0
21	1761.0
22	1821.0
23	2106.5
24	2392.0
25	3051.5
26	4733.5
27	5756.0
28	6486.5
29	7217.0
30	8594.5
31	9972.0
32	11788.5
33	13605.0
34	15323.0
35	17041.0
36	18331.5
37	19622.0
38	22053.5
39	24485.0
40	26418.0
41	28351.0
42	31760.0
43	35169.0
44	38971.0
45	42773.0
46	48864.5
47	54956.0
48	60541.0
49	66126.0
50	65353.5
51	64581.0
52	56800.0
53	49019.0
54	44094.5
55	39170.0
56	37457.0
57	35744.0
58	33845.5
59	31947.0
60	29287.5
61	26628.0
62	24230.5
63	21833.0
64	18721.0
65	15609.0
66	13261.5
67	10914.0
68	9592.5
69	8271.0
70	6650.5
71	5030.0
72	4317.0
73	3604.0
74	2791.0
75	1421.0
76	864.0
77	705.0
78	546.0
79	428.5
80	311.0
81	239.5
82	168.0
83	112.5
84	57.0
85	40.0
86	23.0
87	14.0
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.625379188569276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49172235624988	24.303206882822757
2	9.596994560196002	6.2621117319406245
3	3.5356870183758784	3.4605938899984476
4	1.7537485312680405	2.2886684333606504
5	1.1048664430734343	1.8023343328998291
6	0.8289677531872705	1.6227232369698605
7	0.6242732400857243	1.4257005822551454
8	0.5351971660820939	1.3968808387260803
9	0.46035150425198357	1.35172281476243
>10	6.882611527494268	49.404993880453205
>50	0.13837503746043103	2.812904488112809
>100	0.043502520135802296	2.516364261773611
>500	0.0018511710696086084	0.40842225657612014
>1k	0.0018511710696086084	0.9433723693484654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1872	0.28182839180168434	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1608	0.24208336218862628	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1552	0.23365259833131097	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1216	0.1830680151874189	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	737	0.11095487433645372	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	716	0.10779333788996047	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	700	0.1053845482164418	RNA PCR Primer, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5054935459491686E-4	0.0	0.0	0.19315482194527833	0.0
2	1.5054935459491686E-4	0.0	0.0	0.9221147968938657	0.0
3	1.5054935459491686E-4	0.0	0.0	1.3151991617411936	0.0
4	1.5054935459491686E-4	0.0	0.0	1.7788911738935376	0.0
5	1.5054935459491686E-4	0.0	0.0	2.9972871006301998	0.0
6	1.5054935459491686E-4	0.0	0.0	3.837352499269836	0.0
7	1.5054935459491686E-4	0.0	0.0	4.545988311348109	0.0
8	1.5054935459491686E-4	0.0	0.0	5.616845870581753	0.0
9	1.5054935459491686E-4	0.0	0.0	6.049825814396733	0.0
10	1.5054935459491686E-4	0.0	0.0	6.987748293523065	0.0
11	1.5054935459491686E-4	0.0	0.0	8.272385936281491	0.0
12	1.5054935459491686E-4	0.0	0.0	9.30635890363938	0.0
13	1.5054935459491686E-4	0.0	0.0	9.854358554364877	0.0
14	1.5054935459491686E-4	0.0	0.0	10.135283650038993	0.0
15	1.5054935459491686E-4	0.0	0.0	10.493892212684084	0.0
16	3.010987091898337E-4	0.0	0.0	11.121532471990292	0.0
17	3.010987091898337E-4	0.0	0.0	11.864493536916207	0.0
18	3.010987091898337E-4	0.0	0.0	12.743852317105116	0.0
19	3.010987091898337E-4	0.0	0.0	13.228771788255344	0.0
20	3.010987091898337E-4	0.0	0.0	13.757049473528907	0.0
21	3.010987091898337E-4	0.0	0.0	14.344944703222057	0.0
22	3.010987091898337E-4	0.0	0.0	14.940367400644954	0.0
23	3.010987091898337E-4	0.0	0.0	15.510196707786713	0.0
24	3.010987091898337E-4	0.0	0.0	15.998879912801813	0.0
25	3.010987091898337E-4	0.0	0.0	16.44330160756601	0.0
26	3.010987091898337E-4	0.0	0.0	16.86288265882204	0.0
27	3.010987091898337E-4	0.0	0.0	17.352619709319306	0.0
28	3.010987091898337E-4	0.0	0.0	17.7792765802413	0.0
29	3.010987091898337E-4	0.0	0.0	18.235742223373087	0.0
30	3.010987091898337E-4	0.0	0.0	18.790968243119142	0.0
31	3.010987091898337E-4	0.0	0.0	19.242164658840107	0.0
32	3.010987091898337E-4	0.0	0.0	19.68131712619348	0.0
33	3.010987091898337E-4	0.0	0.0	20.168645387017225	0.0
34	6.021974183796675E-4	0.0	0.0	20.70700987904865	0.0
35	6.021974183796675E-4	0.0	0.0	21.2267062511103	0.0
36	6.021974183796675E-4	0.0	0.0	21.649749937522017	0.0
37	6.021974183796675E-4	0.0	0.0	22.109678215809488	0.0
38	6.021974183796675E-4	0.0	0.0	22.590833953094844	0.0
39	6.021974183796675E-4	0.0	0.0	23.305190640647723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTA	25	3.8890204E-5	45.000004	12
TTACGCG	25	3.8890204E-5	45.000004	1
ACCGGTA	20	7.031133E-4	45.0	41
CGTTAGA	20	7.031133E-4	45.0	9
GCGATAT	45	3.8380676E-10	45.0	9
ATGCGGC	20	7.031133E-4	45.0	42
TATCGCC	20	7.031133E-4	45.0	17
ATCGCAC	20	7.031133E-4	45.0	33
CTTACGT	20	7.031133E-4	45.0	22
GCGTAAG	80	0.0	42.1875	1
CACGGGT	65	0.0	41.53846	4
CGTAAGG	115	0.0	41.08696	2
ACGGGTC	50	1.0804797E-9	40.500004	5
ATTAACG	35	6.244307E-6	38.57143	1
TTCGAAG	35	6.244307E-6	38.57143	1
AAATGCG	35	6.244307E-6	38.57143	1
CGAATGG	100	0.0	38.250004	2
CGTTTTT	855	0.0	38.157894	1
CGGGCAC	65	9.094947E-12	38.07692	6
GCTACGA	95	0.0	37.894737	10
>>END_MODULE