##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542266
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071247321425275	33.0	31.0	34.0	30.0	34.0
2	32.15154555144523	33.0	31.0	34.0	30.0	34.0
3	32.02696647033006	33.0	31.0	34.0	30.0	34.0
4	35.767342964522946	37.0	35.0	37.0	35.0	37.0
5	35.84673573486075	37.0	35.0	37.0	35.0	37.0
6	35.773756790947616	37.0	35.0	37.0	35.0	37.0
7	36.02323951713735	37.0	35.0	37.0	35.0	37.0
8	35.86310039722203	37.0	35.0	37.0	35.0	37.0
9	37.74087809303921	39.0	38.0	39.0	35.0	39.0
10	37.47798313005057	39.0	37.0	39.0	35.0	39.0
11	37.39384914414697	39.0	37.0	39.0	35.0	39.0
12	37.151829544909695	39.0	37.0	39.0	34.0	39.0
13	37.07557914381503	39.0	37.0	39.0	33.0	39.0
14	38.10348426786854	40.0	37.0	41.0	33.0	41.0
15	38.22043240771134	40.0	37.0	41.0	33.0	41.0
16	38.061019868477835	40.0	36.0	41.0	33.0	41.0
17	38.075245359288616	40.0	36.0	41.0	33.0	41.0
18	37.8384814832573	39.0	36.0	41.0	33.0	41.0
19	37.50907672618235	39.0	36.0	41.0	33.0	41.0
20	37.40303098479344	39.0	35.0	41.0	33.0	41.0
21	37.32183282743156	39.0	35.0	41.0	33.0	41.0
22	37.43792345454076	39.0	35.0	41.0	33.0	41.0
23	37.477059229234364	39.0	35.0	41.0	33.0	41.0
24	37.40319142265973	39.0	35.0	41.0	33.0	41.0
25	37.142629263129166	39.0	35.0	41.0	33.0	41.0
26	37.18353723080555	39.0	35.0	41.0	33.0	41.0
27	37.18202505781296	39.0	35.0	41.0	33.0	41.0
28	37.01591469869031	39.0	35.0	41.0	33.0	41.0
29	36.90823138459723	39.0	35.0	41.0	33.0	41.0
30	36.60651967853415	38.0	35.0	41.0	32.0	41.0
31	36.342968211173115	38.0	35.0	41.0	31.0	41.0
32	36.00224797424142	38.0	35.0	41.0	30.0	41.0
33	35.5778437150771	38.0	35.0	41.0	27.0	41.0
34	35.1669844688769	38.0	35.0	41.0	23.0	41.0
35	34.88066926563716	38.0	34.0	41.0	22.0	41.0
36	34.69835468201953	38.0	34.0	41.0	21.0	41.0
37	34.65946417440887	38.0	34.0	41.0	21.0	41.0
38	34.528720222178784	38.0	34.0	40.0	21.0	41.0
39	34.41158029454179	38.0	34.0	40.0	20.0	41.0
40	34.26514662545688	37.0	34.0	40.0	19.0	41.0
41	34.06646737947797	37.0	34.0	40.0	18.0	41.0
42	34.06016973219785	37.0	34.0	40.0	18.0	41.0
43	33.98701190928438	37.0	34.0	40.0	18.0	41.0
44	33.863841730811075	37.0	34.0	40.0	18.0	41.0
45	33.832884599071306	36.0	33.0	40.0	19.0	41.0
46	33.72604035657777	36.0	33.0	40.0	19.0	41.0
47	33.64126093098221	36.0	33.0	40.0	20.0	41.0
48	33.54781048415354	35.0	33.0	40.0	19.0	41.0
49	33.50948243113159	35.0	33.0	40.0	19.0	41.0
50	33.37834937097292	35.0	33.0	40.0	18.0	41.0
51	32.28316914576979	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	13.0
10	15.0
11	20.0
12	14.0
13	15.0
14	23.0
15	35.0
16	79.0
17	163.0
18	258.0
19	525.0
20	850.0
21	1334.0
22	1985.0
23	2760.0
24	4717.0
25	7842.0
26	11865.0
27	14100.0
28	13400.0
29	12144.0
30	11499.0
31	12724.0
32	15021.0
33	20762.0
34	40237.0
35	48947.0
36	40309.0
37	54706.0
38	90528.0
39	135288.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.493407294574986	17.46283189431017	20.772831046018005	24.270929765096835
2	40.44915226106745	22.36485414907075	21.404808710116438	15.781184879745364
3	28.1725204973205	22.15517845485426	34.70861164078146	14.963689407043775
4	24.91507857767221	21.913415187380362	35.89271685851593	17.278789376431494
5	23.34979511900064	25.436593848775324	33.324420118539614	17.889190913684427
6	22.89024205832562	29.88809919854831	32.995245875640364	14.226412867485699
7	69.9728546506696	4.899440495992741	20.61349964777434	4.5142052055633215
8	71.18038010865516	5.151161975856867	19.328337015413098	4.340120900074871
9	66.37683350975352	5.352907982429287	20.819302703839078	7.450955803978121
10	41.396657728863694	18.854953104196095	25.948150907488206	13.800238259452001
11	32.18383597717725	18.9067726908934	31.155189519534694	17.754201812394655
12	28.292203457343813	17.23526830005938	35.78981533048356	18.682712912113246
13	25.691265910088408	17.275654383641974	37.90980810155901	19.123271604710602
14	19.94316442483947	20.330243828674487	39.13005794204321	20.59653380444284
15	19.367616630952337	19.697528519213815	41.119671895342876	19.81518295449097
16	25.230053147348347	19.959945856830412	36.045225037158886	18.764775958662355
17	24.388215377692866	21.46418916177669	35.169639992180954	18.97795546834948
18	25.34088436302479	21.0522142269661	34.668225557198866	18.938675852810245
19	23.80768847761062	24.298407054840244	32.44108979725817	19.452814670290962
20	25.11276753475232	24.47525753043709	32.899167567208714	17.51280736760188
21	25.021483921175218	24.424728823123708	33.56802749941911	16.985759756281972
22	23.070780760733662	21.166549258113175	35.69281496534911	20.069855015804052
23	22.507957349345155	21.828954793403977	36.156978309538125	19.506109547712747
24	24.11915923181612	20.653885731356937	34.91754968963571	20.30940534719123
25	24.80941087953145	21.359627931679288	32.377467884765046	21.453493304024224
26	22.303998406686016	20.360487288526294	33.75188560595722	23.583628698830463
27	22.031991679360313	20.009736918781556	35.22570103971114	22.73257036214699
28	20.526273083689556	23.17257582072267	33.300999878288515	23.00015121729926
29	24.040600000737644	23.534759693582117	32.038888663497254	20.385751642182985
30	24.9064112446659	22.453556003880017	33.27020318441503	19.369829567039055
31	25.900572781623776	22.67724695997905	31.550198611013787	19.87198164738339
32	28.309169300675315	22.7600476518904	30.43155941917804	18.499223628256242
33	27.225937086227052	24.122478635946194	29.22145220242464	19.43013207540211
34	24.683089111247984	25.776463949427036	30.874884281883798	18.665562657441182
35	23.948947564479425	27.488354423843646	29.76288389830821	18.799814113368715
36	24.11823717511332	30.233317228076256	26.550069523075393	19.09837607373503
37	24.2091519660093	29.23104159213375	26.54250865811244	20.01729778374451
38	24.819737914602797	28.161824639568035	26.391106947512842	20.627330498316322
39	24.809964113553125	25.989827870454725	26.568510657131373	22.63169735886078
40	25.975259374550497	23.685239347478912	28.808370799570692	21.5311304783999
41	21.78838429848082	25.280213031980615	28.49745327938687	24.433949390151696
42	22.23816355810617	24.62315542556605	29.65186827129121	23.48681274503657
43	22.12714793108917	23.230112158977327	29.987312499769487	24.655427410164016
44	22.90849878104104	22.723349795119	29.94194730999177	24.426204113848186
45	22.89706527792633	22.554797829847345	29.501204206053856	25.046932686172468
46	22.958658665673305	23.87094156742263	29.687644071359813	23.482755695544252
47	21.311680983133737	24.015888881102633	31.92344716430682	22.74898297145681
48	21.31131216045262	23.61387215868227	31.9230783416257	23.151737339239414
49	22.489885037970296	22.529533476190654	32.3427985527398	22.637782933099253
50	20.9600085566862	22.456690996669533	32.87500968159538	23.708290765048886
51	20.240804328502985	21.7325076622912	32.80253602475538	25.224151984450437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302.0
1	685.5
2	1069.0
3	11915.5
4	22762.0
5	14665.0
6	6568.0
7	6361.5
8	6155.0
9	6091.0
10	6027.0
11	5857.0
12	5687.0
13	5427.0
14	5167.0
15	4764.5
16	4362.0
17	4110.0
18	3858.0
19	3626.5
20	3395.0
21	3130.5
22	2866.0
23	2837.0
24	2808.0
25	2924.0
26	3240.0
27	3440.0
28	3691.5
29	3943.0
30	4518.0
31	5093.0
32	5805.0
33	6517.0
34	7717.0
35	8917.0
36	9622.5
37	10328.0
38	11151.5
39	11975.0
40	13153.5
41	14332.0
42	15370.5
43	16409.0
44	18538.5
45	20668.0
46	29396.0
47	38124.0
48	37841.5
49	37559.0
50	38622.5
51	39686.0
52	37550.5
53	35415.0
54	34482.5
55	33550.0
56	33499.5
57	33449.0
58	33274.0
59	33099.0
60	32230.5
61	31362.0
62	28826.0
63	26290.0
64	23214.0
65	20138.0
66	16698.5
67	13259.0
68	11411.0
69	9563.0
70	7800.0
71	6037.0
72	5027.0
73	4017.0
74	3253.0
75	1929.5
76	1370.0
77	986.5
78	603.0
79	454.5
80	306.0
81	222.5
82	139.0
83	101.0
84	63.0
85	54.5
86	46.0
87	24.5
88	3.0
89	3.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	542266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.2798773166263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13834565112718	30.614374633064152
2	10.45159795867062	9.255909507452738
3	5.771876690187836	7.667339751846374
4	3.948185845806499	6.993007395022088
5	2.814672596190535	6.2316678622893455
6	2.115271465996238	5.61983766034042
7	1.5680312463668962	4.860256185243395
8	1.11629420744988	3.954349644411302
9	0.8395156091393715	3.345628336027575
>10	2.1806331772083984	13.097487782359215
>50	0.0284145678896826	0.9009036087192681
>100	0.0213109258816967	1.9938667642908963
>500	0.0029250290425858216	0.9349488455792409
>1k	0.002507167750787847	1.9074584700329296
>5k	0.0	0.0
>10k+	4.1786129179797457E-4	2.6229635533210605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14176	2.614215163775712	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	3495	0.6445176352564977	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	1513	0.27901435826697596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	1443	0.26610556442779004	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	1367	0.25209030254524534	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	1293	0.2384438633438202	RNA PCR Primer, Index 40 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	1198	0.22092478599063928	RNA PCR Primer, Index 40 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT	983	0.18127634777028248	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	898	0.16560138382269957	Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	866	0.15970022092478597	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	720	0.13277616520305532	RNA PCR Primer, Index 40 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	557	0.10271711669180808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8441134055979908E-4	0.0	0.0	0.07542423828895782	0.0
2	1.8441134055979908E-4	0.0	0.0	0.3415298027167479	0.0
3	1.8441134055979908E-4	0.0	0.0	0.6463617486620957	0.0
4	1.8441134055979908E-4	0.0	0.0	1.1557058712882606	0.0
5	1.8441134055979908E-4	0.0	0.0	2.4987736645852774	0.0
6	1.8441134055979908E-4	0.0	0.0	3.673842726632317	0.0
7	1.8441134055979908E-4	0.0	0.0	4.609545868632737	0.0
8	1.8441134055979908E-4	0.0	0.0	5.710297160434178	0.0
9	1.8441134055979908E-4	0.0	0.0	6.5543478661763785	0.0
10	1.8441134055979908E-4	0.0	0.0	7.765930373654259	0.0
11	1.8441134055979908E-4	0.0	0.0	8.615329008272692	0.0
12	1.8441134055979908E-4	0.0	0.0	9.442413870683392	0.0
13	1.8441134055979908E-4	0.0	0.0	9.78431249608126	0.0
14	1.8441134055979908E-4	0.0	0.0	10.02201871406284	0.0
15	1.8441134055979908E-4	0.0	0.0	10.221920607229663	0.0
16	1.8441134055979908E-4	0.0	0.0	10.41665898286081	0.0
17	1.8441134055979908E-4	0.0	0.0	10.612319415194756	0.0
18	1.8441134055979908E-4	0.0	0.0	10.83969859810499	0.0
19	1.8441134055979908E-4	0.0	0.0	11.065233667609624	0.0
20	3.6882268111959816E-4	0.0	0.0	11.254624114364537	0.0
21	3.6882268111959816E-4	0.0	0.0	11.448440433292886	0.0
22	3.6882268111959816E-4	0.0	0.0	11.70182161522205	0.0
23	3.6882268111959816E-4	0.0	0.0	11.935286372370756	0.0
24	5.532340216793972E-4	0.0	0.0	12.153998222274677	0.0
25	5.532340216793972E-4	0.0	0.0	12.345048371094629	0.0
26	5.532340216793972E-4	0.0	0.0	12.539233512704097	0.0
27	5.532340216793972E-4	0.0	0.0	12.752044199710106	0.0
28	5.532340216793972E-4	0.0	0.0	12.944569639254535	0.0
29	5.532340216793972E-4	0.0	0.0	13.153692099449348	0.0
30	5.532340216793972E-4	0.0	0.0	13.442664670106552	0.0
31	5.532340216793972E-4	0.0	0.0	13.66506474682167	0.0
32	5.532340216793972E-4	0.0	0.0	13.933198835995618	0.0
33	5.532340216793972E-4	0.0	0.0	14.178281507599591	0.0
34	7.376453622391963E-4	0.0	0.0	14.448444121519698	0.0
35	7.376453622391963E-4	0.0	0.0	14.750509897356649	0.0
36	7.376453622391963E-4	0.0	0.0	15.045936864933445	0.0
37	7.376453622391963E-4	0.0	0.0	15.34560529334312	0.0
38	7.376453622391963E-4	0.0	0.0	15.786717219962158	0.0
39	7.376453622391963E-4	0.0	0.0	16.379968502543033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1633732E-6	45.000004	1
CGGTCTA	35	1.2103374E-7	45.000004	31
CCGATCG	20	7.029988E-4	45.0	10
TACGGAT	20	7.029988E-4	45.0	13
AATTGCG	20	7.029988E-4	45.0	1
CGTTTTT	7010	0.0	43.716118	1
CCATACG	40	3.4537152E-7	39.375	2
TATACGG	40	3.4537152E-7	39.375	2
ATGGGAC	155	0.0	39.193546	5
ACCGGTA	35	6.242186E-6	38.57143	41
TTTACGG	35	6.242186E-6	38.57143	2
TTAGACG	35	6.242186E-6	38.57143	1
TACCGGT	35	6.242186E-6	38.57143	40
GTTTTTT	7985	0.0	38.49092	2
ACGGGTA	30	1.1391267E-4	37.500004	5
GCGATAT	30	1.1391267E-4	37.500004	9
CGTAAGG	60	1.546141E-10	37.500004	2
TCGACGG	30	1.1391267E-4	37.500004	2
CGGTAGT	145	0.0	37.24138	12
TACGAAT	85	0.0	37.058826	12
>>END_MODULE