##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935589.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992813
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14040811310891	33.0	31.0	34.0	30.0	34.0
2	32.17086399956487	33.0	31.0	34.0	30.0	34.0
3	32.14036782354784	33.0	31.0	34.0	30.0	34.0
4	35.81063906294539	37.0	35.0	37.0	35.0	37.0
5	35.86722272975878	37.0	35.0	37.0	35.0	37.0
6	35.792768628130375	37.0	35.0	37.0	35.0	37.0
7	36.04297888927724	37.0	35.0	37.0	35.0	37.0
8	35.86537746786152	37.0	35.0	37.0	35.0	37.0
9	37.71268909653681	39.0	38.0	39.0	35.0	39.0
10	37.391703170687734	39.0	37.0	39.0	34.0	39.0
11	37.32698403425419	39.0	37.0	39.0	34.0	39.0
12	37.10653265015667	39.0	37.0	39.0	34.0	39.0
13	36.98350545369571	39.0	37.0	39.0	33.0	39.0
14	37.929909257836066	40.0	37.0	41.0	33.0	41.0
15	38.06683333115098	40.0	37.0	41.0	33.0	41.0
16	37.984559025717836	40.0	36.0	41.0	33.0	41.0
17	38.00741730819399	40.0	36.0	41.0	33.0	41.0
18	37.881433865189116	40.0	36.0	41.0	33.0	41.0
19	37.6999324142613	39.0	36.0	41.0	33.0	41.0
20	37.72880995716213	40.0	35.0	41.0	33.0	41.0
21	37.69803880489075	40.0	35.0	41.0	33.0	41.0
22	37.84080587180063	40.0	35.0	41.0	34.0	41.0
23	37.86597073164835	40.0	35.0	41.0	34.0	41.0
24	37.797765540942756	40.0	35.0	41.0	34.0	41.0
25	37.556705039116125	39.0	35.0	41.0	33.0	41.0
26	37.60420542438506	40.0	35.0	41.0	33.0	41.0
27	37.599773572666756	40.0	35.0	41.0	33.0	41.0
28	37.46152699450954	40.0	35.0	41.0	33.0	41.0
29	37.390654634860745	40.0	35.0	41.0	33.0	41.0
30	37.21064994112688	39.0	35.0	41.0	33.0	41.0
31	37.11970129319419	39.0	35.0	41.0	32.0	41.0
32	36.959959226964195	39.0	35.0	41.0	32.0	41.0
33	36.815908937534054	39.0	35.0	41.0	32.0	41.0
34	36.55779487174322	39.0	35.0	41.0	31.0	41.0
35	36.32463716732154	39.0	35.0	41.0	30.0	41.0
36	36.220478579551234	39.0	35.0	41.0	30.0	41.0
37	36.2382946234588	39.0	35.0	41.0	30.0	41.0
38	36.09087914843984	39.0	35.0	41.0	30.0	41.0
39	36.01036650406471	39.0	35.0	41.0	30.0	41.0
40	35.8511330935433	39.0	35.0	41.0	29.0	41.0
41	35.67199059641644	38.0	35.0	41.0	28.0	41.0
42	35.68298763211199	38.0	35.0	41.0	28.0	41.0
43	35.68089257493607	38.0	35.0	41.0	29.0	41.0
44	35.62339332784724	38.0	35.0	41.0	28.0	41.0
45	35.60471911628877	38.0	35.0	40.0	28.0	41.0
46	35.51456719442634	38.0	35.0	40.0	28.0	41.0
47	35.400180094338005	38.0	35.0	40.0	28.0	41.0
48	35.30114331701942	37.0	35.0	40.0	27.0	41.0
49	35.26826703518185	37.0	35.0	40.0	27.0	41.0
50	35.14174673377565	37.0	34.0	40.0	27.0	41.0
51	34.00911652043235	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	22.0
10	28.0
11	41.0
12	35.0
13	36.0
14	45.0
15	73.0
16	97.0
17	183.0
18	360.0
19	672.0
20	1320.0
21	2069.0
22	3202.0
23	4394.0
24	5939.0
25	8705.0
26	11852.0
27	13351.0
28	13991.0
29	14560.0
30	16322.0
31	20114.0
32	26369.0
33	38307.0
34	75585.0
35	81844.0
36	71684.0
37	102696.0
38	176017.0
39	302673.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83193310321279	19.36316305286091	22.492654709396433	15.312249134529864
2	32.96975361926163	26.92501004720929	23.558313599842066	16.54692273368701
3	29.976037783550375	26.4459671660222	27.705217397435366	15.872777652992054
4	26.741188924802557	23.359081720323967	31.334601783014527	18.565127571858948
5	27.100068190082123	27.298091382767954	26.612363053263806	18.989477373886118
6	24.586704646292905	33.88885923129532	26.589800899061554	14.934635223350218
7	76.01602718739582	5.376037582102571	14.204185481052322	4.403749749449292
8	77.27155063440951	6.995879385141008	11.220844207317993	4.511725773131496
9	72.06321835028349	6.015735088078017	12.848441750863456	9.07260481077504
10	44.14960319818536	20.91330391523882	19.668759373618194	15.268333512957627
11	33.4861650683462	22.97129469497277	25.08951836851451	18.453021868166513
12	29.01321799774983	19.996716400772353	30.461325546704167	20.52874005477366
13	27.043662804576492	20.084749091722205	33.86176450147208	19.009823602229222
14	21.412693024769013	24.522442796377568	33.199303393489004	20.865560785364416
15	20.05010006919732	22.75040717637662	36.927397203703016	20.272095550723044
16	26.06523081385921	22.527605903629386	30.06920739353735	21.337955888974054
17	26.04780557869407	23.424350809266194	28.941704026840902	21.586139585198826
18	26.58657773417552	22.471905585442574	29.982282665517072	20.959234014864833
19	25.55919392675156	25.396021204395996	27.467609710992907	21.57717515785954
20	25.96783079995931	26.459766340690543	28.76634371226001	18.80605914709014
21	24.891193004120616	26.339502000880326	30.366947249884923	18.402357745114134
22	23.881738051375233	21.91560747089331	32.20364761541197	21.99900686231949
23	23.185332988186094	24.351514333514974	31.747166888427124	20.71598578987181
24	24.40570379316145	24.02909712100869	29.67577982963559	21.889419256194266
25	24.865609132837704	25.72115796227487	27.274219817830748	22.13901308705668
26	22.468783144459227	23.322720391453373	28.975144362533527	25.23335210155387
27	22.514209624571798	23.759056337900493	30.2201925236676	23.506541513860114
28	21.374115770039275	24.69014809435412	29.892134772610753	24.04360136299585
29	23.121373309978818	26.4202825708366	28.38590953180508	22.072434587379497
30	24.643311479603913	25.467736623110294	29.336138829769553	20.55281306751624
31	27.07156332562124	23.554687539345274	27.643372921184557	21.73037621384893
32	28.216089031872066	24.46069904402944	26.437909253807113	20.885302670291384
33	28.255774249531385	23.080479405487235	26.33517087306472	22.328575471916665
34	25.933483949142488	24.54399771155293	28.92065273117898	20.6018656081256
35	23.82069936634593	26.894289256889266	28.775912483015432	20.50909889374938
36	25.670393115319808	27.665028560262606	27.26142788218929	19.403150442228295
37	23.89130682213065	27.04849755190554	27.312998520365873	21.74719710559793
38	26.07953360804099	25.676235101675744	26.398123312245104	21.84610797803816
39	25.475190191909252	23.892011889449474	26.851985217760046	23.780812700881235
40	26.102901553464754	22.984187354516912	29.26442341105525	21.648487680963083
41	22.962028095925415	24.267107703061903	28.896982614047158	23.87388158696552
42	21.929205197756275	24.142713683241457	31.135168455691055	22.792912663311217
43	22.843274614655527	23.414076971191957	29.68111819647809	24.061530217674427
44	23.79048219553934	22.972402657902343	28.644367066104092	24.592748080454225
45	22.645150698067006	22.556815835409086	28.170259656148737	26.627773810375167
46	24.21644358000953	24.637167321539906	27.58978780495421	23.556601293496357
47	21.582614248604724	24.19146405214275	31.580569553380144	22.645352145872387
48	21.768752020773295	25.17624164872942	29.36484514203581	23.690161188461474
49	23.200643021394765	23.325641384631346	30.607375205602665	22.866340388371224
50	22.198339465740276	23.72914133880197	30.1351815497984	23.937337645659355
51	21.659466586356142	23.766006287186006	28.899601435517063	25.674925690940793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511.0
1	1321.0
2	2131.0
3	8606.0
4	15081.0
5	9649.5
6	4218.0
7	4112.5
8	4007.0
9	4039.5
10	4072.0
11	3980.5
12	3889.0
13	3809.5
14	3730.0
15	3534.0
16	3338.0
17	3316.5
18	3295.0
19	3101.5
20	2908.0
21	3070.5
22	3233.0
23	3597.0
24	3961.0
25	4575.5
26	6404.0
27	7618.0
28	8395.0
29	9172.0
30	10730.5
31	12289.0
32	14847.5
33	17406.0
34	19875.5
35	22345.0
36	23798.0
37	25251.0
38	28350.5
39	31450.0
40	33111.5
41	34773.0
42	38101.0
43	41429.0
44	45698.5
45	49968.0
46	71282.0
47	92596.0
48	85266.5
49	77937.0
50	78465.5
51	78994.0
52	73108.0
53	67222.0
54	65012.5
55	62803.0
56	62197.0
57	61591.0
58	59154.5
59	56718.0
60	52152.0
61	47586.0
62	44249.0
63	40912.0
64	36257.0
65	31602.0
66	27050.0
67	22498.0
68	19114.5
69	15731.0
70	12863.0
71	9995.0
72	8261.5
73	6528.0
74	5536.0
75	3403.5
76	2263.0
77	1667.5
78	1072.0
79	826.5
80	581.0
81	385.0
82	189.0
83	161.5
84	134.0
85	81.0
86	28.0
87	20.0
88	12.0
89	9.0
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	992813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.67727792932558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20228614943034	20.992423049692345
2	8.41289033599903	4.82517588708763
3	3.2941750213170575	2.834039179024539
4	1.8864699756924574	2.163952951930427
5	1.299872865364435	1.8638407716422358
6	1.027040084505492	1.7671628368753214
7	0.8705748251661769	1.7476001353695136
8	0.7387067743952738	1.69472795820871
9	0.6617103643567959	1.7078446824637616
>10	8.41795715482448	50.876336897233465
>50	0.15051185142687634	2.625426874926239
>100	0.02967837562444398	1.5322750098008693
>500	0.0038864539508194533	0.8060072540477967
>1k	0.0028265119642323297	1.3257831450930762
>5k	0.0014132559821161648	3.2374033666040485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	9721	0.9791370580361055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9492	0.9560712843204108	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	6474	0.6520865460061462	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	6265	0.6310352503442239	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	2726	0.2745733587291867	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	2290	0.23065773715694698	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	2247	0.2263266093413362	TruSeq Adapter, Index 21 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1306	0.1315454169113418	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	1259	0.12681139348497653	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	1149	0.11573176418922797	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1060	0.10676733684994051	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	1048	0.10555865001767706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0289561075449256E-4	0.0	0.0	0.08067984605358713	0.0
2	5.036195134431157E-4	0.0	0.0	0.4625241611461574	0.0
3	6.043434161317388E-4	0.0	0.0	0.7036571841827212	0.0
4	6.043434161317388E-4	0.0	0.0	1.2062694585989506	0.0
5	6.043434161317388E-4	0.0	0.0	2.77403700394737	0.0
6	6.043434161317388E-4	0.0	0.0	3.7124816052972713	0.0
7	6.043434161317388E-4	0.0	0.0	4.358726164947478	0.0
8	6.043434161317388E-4	0.0	0.0	5.153840652771469	0.0
9	6.043434161317388E-4	0.0	0.0	5.609314140729422	0.0
10	7.050673188203619E-4	0.0	0.0	6.888104809264182	0.0
11	8.057912215089851E-4	0.0	0.0	7.6017336598130765	0.0
12	8.057912215089851E-4	0.0	0.0	8.79692348911628	0.0
13	8.057912215089851E-4	0.0	0.0	9.104836459635399	0.0
14	8.057912215089851E-4	0.0	0.0	9.299636487435198	0.0
15	8.057912215089851E-4	0.0	0.0	9.550036109519114	0.0
16	8.057912215089851E-4	0.0	0.0	9.81373128675793	0.0
17	8.057912215089851E-4	0.0	0.0	10.106434947971069	0.0
18	8.057912215089851E-4	0.0	0.0	10.437212244400506	0.0
19	8.057912215089851E-4	0.0	0.0	10.771011257910603	0.0
20	8.057912215089851E-4	0.0	0.0	11.028562277085413	0.0
21	8.057912215089851E-4	0.0	0.0	11.310387756808181	0.0
22	8.057912215089851E-4	0.0	0.0	11.662417796704919	0.0
23	8.057912215089851E-4	0.0	0.0	11.944545448135752	0.0
24	9.065151241976082E-4	0.0	0.0	12.19272914436052	0.0
25	9.065151241976082E-4	0.0	0.0	12.427617285430388	0.0
26	9.065151241976082E-4	0.0	0.0	12.685974095826706	0.0
27	9.065151241976082E-4	0.0	0.0	12.985023362909228	0.0
28	9.065151241976082E-4	0.0	0.0	13.232099096204422	0.0
29	0.0010072390268862314	0.0	0.0	13.509996343722332	0.0
30	0.0010072390268862314	0.0	0.0	13.878242931951938	0.0
31	0.0010072390268862314	0.0	0.0	14.145362721882167	0.0
32	0.0010072390268862314	0.0	0.0	14.446426467018462	0.0
33	0.0011079629295748545	0.0	0.0	14.73691420237245	0.0
34	0.0011079629295748545	0.0	0.0	15.03324392408238	0.0
35	0.0011079629295748545	0.0	0.0	15.34790539608164	0.0
36	0.0011079629295748545	0.0	0.0	15.634867794841526	0.0
37	0.0011079629295748545	0.0	0.0	15.951241573186492	0.0
38	0.0011079629295748545	0.0	0.0	16.365720432750177	0.0
39	0.0011079629295748545	0.0	0.0	17.055880613972622	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	70	0.0	45.000004	5
AATCCCG	20	7.0328225E-4	45.0	25
ACGTTAG	20	7.0328225E-4	45.0	1
GTAATCG	20	7.0328225E-4	45.0	1
ACTCGAT	20	7.0328225E-4	45.0	33
CGGTATT	45	3.8380676E-10	45.0	43
GCGTAAT	30	2.1652031E-6	44.999996	1
CGTTTTT	4245	0.0	41.819786	1
CTAACGG	55	6.002665E-11	40.909092	2
CCGGTAT	50	1.0804797E-9	40.5	42
CGAATAT	85	0.0	39.705883	14
CGGGTAT	80	0.0	39.375	6
TCGTTAG	35	6.2474282E-6	38.57143	1
ATCGGCA	35	6.2474282E-6	38.57143	41
TACAACG	35	6.2474282E-6	38.57143	1
CGATGAA	550	0.0	38.454544	19
CGTTAGG	95	0.0	37.894737	2
GCGATAT	30	1.13981005E-4	37.499996	9
GCGAACC	55	2.748493E-9	36.81818	33
TATTGCG	55	2.748493E-9	36.81818	1
>>END_MODULE