##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756835
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17410532018207	33.0	31.0	34.0	30.0	34.0
2	32.19428541227612	33.0	31.0	34.0	30.0	34.0
3	32.20955426215754	34.0	31.0	34.0	30.0	34.0
4	35.85056848586548	37.0	35.0	37.0	35.0	37.0
5	35.9164599945827	37.0	35.0	37.0	35.0	37.0
6	35.84934629080315	37.0	35.0	37.0	35.0	37.0
7	36.08591436706812	37.0	35.0	37.0	35.0	37.0
8	35.88103087198663	37.0	35.0	37.0	35.0	37.0
9	37.68490754259515	39.0	38.0	39.0	35.0	39.0
10	37.41556481928029	39.0	37.0	39.0	34.0	39.0
11	37.37439071924528	39.0	37.0	39.0	35.0	39.0
12	37.22009024424082	39.0	37.0	39.0	34.0	39.0
13	37.15446695779133	39.0	37.0	39.0	34.0	39.0
14	38.30708542813163	40.0	37.0	41.0	34.0	41.0
15	38.377744158237924	40.0	37.0	41.0	34.0	41.0
16	38.21230783460067	40.0	37.0	41.0	34.0	41.0
17	38.1856230221911	40.0	37.0	41.0	34.0	41.0
18	38.11739811187379	40.0	37.0	41.0	34.0	41.0
19	38.000383174668194	40.0	37.0	41.0	34.0	41.0
20	38.106597871398655	40.0	36.0	41.0	34.0	41.0
21	38.069311012307836	40.0	36.0	41.0	34.0	41.0
22	38.15842554850133	40.0	37.0	41.0	34.0	41.0
23	38.18319316627799	40.0	37.0	41.0	34.0	41.0
24	38.117190669036184	40.0	36.0	41.0	34.0	41.0
25	37.88639135346542	40.0	36.0	41.0	34.0	41.0
26	37.94573453923246	40.0	36.0	41.0	34.0	41.0
27	37.953912015168434	40.0	36.0	41.0	34.0	41.0
28	37.850084893008386	40.0	36.0	41.0	34.0	41.0
29	37.802859275799875	40.0	36.0	41.0	34.0	41.0
30	37.645640066857375	40.0	36.0	41.0	33.0	41.0
31	37.655609214690124	40.0	36.0	41.0	33.0	41.0
32	37.5704770524619	40.0	35.0	41.0	33.0	41.0
33	37.47972015036302	40.0	35.0	41.0	33.0	41.0
34	37.39401454742447	40.0	35.0	41.0	33.0	41.0
35	37.28530657276685	40.0	35.0	41.0	33.0	41.0
36	37.18732220365073	40.0	35.0	41.0	32.0	41.0
37	37.20115216658849	40.0	35.0	41.0	33.0	41.0
38	37.03140314599616	40.0	35.0	41.0	32.0	41.0
39	36.9952076740637	39.0	35.0	41.0	32.0	41.0
40	36.83364802103497	39.0	35.0	41.0	31.0	41.0
41	36.709835036698884	39.0	35.0	41.0	31.0	41.0
42	36.73130206716127	39.0	35.0	41.0	31.0	41.0
43	36.6858760496013	39.0	35.0	41.0	31.0	41.0
44	36.61213606664597	39.0	35.0	41.0	31.0	41.0
45	36.597276817271926	39.0	35.0	41.0	31.0	41.0
46	36.46083624568103	39.0	35.0	41.0	31.0	41.0
47	36.37573843704374	39.0	35.0	41.0	31.0	41.0
48	36.261926311547434	38.0	35.0	41.0	31.0	41.0
49	36.20463905606903	38.0	35.0	41.0	30.0	41.0
50	36.095163410783066	38.0	35.0	40.0	30.0	41.0
51	34.91055381952473	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	25.0
10	26.0
11	22.0
12	21.0
13	24.0
14	38.0
15	33.0
16	46.0
17	95.0
18	200.0
19	341.0
20	660.0
21	1191.0
22	1756.0
23	2457.0
24	3266.0
25	4379.0
26	5564.0
27	6607.0
28	7437.0
29	8425.0
30	10349.0
31	12894.0
32	17004.0
33	24044.0
34	49204.0
35	65034.0
36	50922.0
37	76326.0
38	141499.0
39	266751.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.488514669643976	19.168510970026492	23.291074012169098	12.051900348160432
2	31.011382930229175	27.144622011402745	24.476008641249415	17.367986417118658
3	31.901537323194617	26.443015981026246	25.40910502289138	16.24634167288775
4	28.65869046753916	24.467552372710035	28.238916012076608	18.634841147674198
5	28.12832387508506	28.501324595189175	23.644387482079978	19.72596404764579
6	25.09992270442038	35.81176874748129	24.230380466019675	14.857928082078656
7	76.60863992812172	6.505116703112303	11.965355724827736	4.920887643938243
8	77.18234489684012	8.707181882444655	9.044771978040128	5.065701242675088
9	70.64128905243547	8.615087832882994	11.29374302192684	9.449880092754695
10	42.838531516116454	25.128066223153	17.86267812667226	14.170724134058283
11	33.943329787866574	24.876227975714656	23.32899509140037	17.851447145018398
12	30.67167876749886	21.73644189288286	27.156117251448464	20.435762088169813
13	26.82579426162902	22.840513454055376	29.168312776232604	21.165379508083003
14	21.126269266088382	27.809628254507256	28.375405471470007	22.68869700793436
15	20.300593920735697	25.198094696994723	33.97239821097069	20.528913171298896
16	23.291998916540592	24.163919480468003	30.532810982578766	22.011270620412642
17	22.91159896146452	25.21500723407348	28.947657019033212	22.925736785428793
18	24.00298611982797	25.57426651780111	28.25556429076351	22.16718307160742
19	25.041521599820303	27.78267389853799	25.917670298017402	21.258134203624305
20	27.85468431031863	25.280675444449585	27.730614995342446	19.13402524988934
21	25.777877608725813	28.267323789201082	26.574220272582533	19.380578329490575
22	23.092615959885578	25.3873037055633	27.935679507422357	23.584400827128764
23	24.685829804382724	26.84191402353221	27.321410875554115	21.15084529653095
24	24.659800352784952	23.542515872019663	28.576373978476155	23.221309796719233
25	22.93009704889441	26.787608924005895	26.51634768476616	23.76594634233353
26	21.60616250569807	25.89098020043999	28.932462161501515	23.57039513236042
27	23.704638395423043	26.198709097755785	27.383379468444247	22.713273038376926
28	20.48649969940608	27.625175896992076	29.422529349197646	22.465795054404197
29	24.018577364947447	25.699260737148784	28.386107936340153	21.896053961563616
30	24.12480923847338	25.516790317572525	28.830722680637127	21.527677763316973
31	25.37712975747686	25.36893774732934	25.498027971750776	23.75590452344302
32	26.82116973977155	26.308112071983984	26.686397959925213	20.18432022831925
33	25.226238215727335	25.342247649751926	27.361446021920234	22.0700681126005
34	23.958194322408453	25.296134560373133	29.40667384568631	21.338997271532104
35	24.068918588595928	24.961319177892143	28.55998995818111	22.40977227533082
36	22.53806972457669	27.78756267878732	28.261377975384335	21.41298962125166
37	25.59910680663553	27.27833675768166	27.206194216705093	19.916362218977714
38	22.60955161957362	26.9460318299233	26.744534806133437	23.699881744369645
39	25.963783387396195	24.7546691154611	27.23275218508658	22.04879531205613
40	23.681912173723465	23.740973924303184	30.6580694603183	21.91904444165505
41	21.412725362859806	25.9319402511776	29.256575079112356	23.398759306850238
42	21.769077804277025	24.87754926767393	29.481326841385506	23.87204608666354
43	22.651172316290868	24.512872686913266	29.026273890610238	23.80968110618563
44	23.382903803338905	24.661518032332015	27.411126599589082	24.54445156474
45	22.997615068013506	24.144100101078834	27.372280615986313	25.486004214921348
46	23.427959859150278	26.617162261259057	27.080142963789992	22.87473491580067
47	21.881123362423775	25.210118453824148	31.18803966518462	21.720718518567455
48	22.427081199997357	23.66143214835466	29.9151069916164	23.99637966003158
49	21.66205315557552	24.21756393401468	30.673792834633705	23.446590075776093
50	21.091915675147156	23.809152589401915	30.138669591126206	24.960262144324723
51	20.634484398845192	23.718247702603605	29.007247286396638	26.640020612154565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	859.0
2	1469.0
3	2710.0
4	3951.0
5	2737.0
6	1523.0
7	1467.5
8	1412.0
9	1457.0
10	1502.0
11	1556.0
12	1610.0
13	1538.0
14	1466.0
15	1624.5
16	1783.0
17	1816.0
18	1849.0
19	2001.0
20	2153.0
21	2257.5
22	2362.0
23	2759.5
24	3157.0
25	3926.0
26	6030.0
27	7365.0
28	8693.5
29	10022.0
30	11607.5
31	13193.0
32	14850.5
33	16508.0
34	18241.5
35	19975.0
36	21659.0
37	23343.0
38	24560.5
39	25778.0
40	29118.5
41	32459.0
42	35600.0
43	38741.0
44	42868.5
45	46996.0
46	59877.5
47	72759.0
48	67447.0
49	62135.0
50	60333.5
51	58532.0
52	52972.5
53	47413.0
54	45006.0
55	42599.0
56	41378.5
57	40158.0
58	39560.5
59	38963.0
60	39223.0
61	39483.0
62	36167.0
63	32851.0
64	27414.5
65	21978.0
66	17865.0
67	13752.0
68	10982.5
69	8213.0
70	7077.5
71	5942.0
72	4821.0
73	3700.0
74	2906.0
75	1816.0
76	1520.0
77	1060.5
78	601.0
79	450.0
80	299.0
81	211.5
82	124.0
83	101.5
84	79.0
85	57.5
86	36.0
87	24.5
88	13.0
89	9.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	756835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.995741576095337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28775605242491	19.051695670307552
2	9.3634723741481	4.868208161865238
3	3.203660981032043	2.4984462888098724
4	1.6458284052680856	1.7113811960778504
5	0.990615444036312	1.287589154222845
6	0.7701088958613718	1.2011731105358603
7	0.6698779045766233	1.2189781026437108
8	0.5671044737212981	1.1793841076405132
9	0.4932403047819364	1.153993274822315
>10	8.539886545570965	52.82058178265474
>50	0.3999201088738119	6.313775841514427
>100	0.05983459429431774	2.8689312444134267
>500	0.005114067888403226	0.9985422880810587
>1k	0.002557033944201613	1.4199747275986936
>5k	0.0010228135776806453	1.4073450488118877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	5303	0.7006811260050076	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	5283	0.6980385420864521	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	4138	0.5467506127491462	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2186	0.28883442229812306	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.25646276929581746	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1414	0.18683068304187836	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	1002	0.13239345431963373	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	916	0.1210303434698448	TruSeq Adapter, Index 21 (95% over 22bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	866	0.11442388367345592	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	794	0.10491058156665589	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	780	0.10306077282366698	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	780	0.10306077282366698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.642583918555564E-4	0.0	0.0	0.13186493753592263	0.0
2	2.642583918555564E-4	0.0	0.0	0.7681991451241024	0.0
3	2.642583918555564E-4	0.0	0.0	1.0373463172289865	0.0
4	2.642583918555564E-4	0.0	0.0	1.4207852438113988	0.0
5	2.642583918555564E-4	0.0	0.0	2.730053446259753	0.0
6	2.642583918555564E-4	0.0	0.0	3.3651984910845827	0.0
7	2.642583918555564E-4	0.0	0.0	3.9288616409124844	0.0
8	2.642583918555564E-4	0.0	0.0	4.674995210316648	0.0
9	2.642583918555564E-4	0.0	0.0	4.9376680518210705	0.0
10	2.642583918555564E-4	0.0	0.0	6.200426777302846	0.0
11	2.642583918555564E-4	0.0	0.0	7.003904417739665	0.0
12	2.642583918555564E-4	0.0	0.0	8.360078484742381	0.0
13	2.642583918555564E-4	0.0	0.0	8.675999392205698	0.0
14	2.642583918555564E-4	0.0	0.0	8.832704618576043	0.0
15	2.642583918555564E-4	0.0	0.0	9.157346052970595	0.0
16	2.642583918555564E-4	0.0	0.0	9.619269721934106	0.0
17	2.642583918555564E-4	0.0	0.0	10.144879663334809	0.0
18	2.642583918555564E-4	0.0	0.0	10.711053267885339	0.0
19	2.642583918555564E-4	0.0	0.0	11.2003276804059	0.0
20	2.642583918555564E-4	0.0	0.0	11.586409190906869	0.0
21	3.9638758778333454E-4	0.0	0.0	12.009090488679831	0.0
22	3.9638758778333454E-4	0.0	0.0	12.509199495266472	0.0
23	3.9638758778333454E-4	0.0	0.0	12.951303784840817	0.0
24	3.9638758778333454E-4	0.0	0.0	13.340424266848125	0.0
25	3.9638758778333454E-4	0.0	0.0	13.687395535354469	0.0
26	3.9638758778333454E-4	0.0	0.0	14.033177641097465	0.0
27	3.9638758778333454E-4	0.0	0.0	14.377374196489328	0.0
28	3.9638758778333454E-4	0.0	0.0	14.720513718313768	0.0
29	3.9638758778333454E-4	0.0	0.0	15.058764459888879	0.0
30	3.9638758778333454E-4	0.0	0.0	15.444185324410208	0.0
31	3.9638758778333454E-4	0.0	0.0	15.797234535929231	0.0
32	5.285167837111127E-4	0.0	0.0	16.15543678608944	0.0
33	5.285167837111127E-4	0.0	0.0	16.50875025600032	0.0
34	5.285167837111127E-4	0.0	0.0	16.86536695580939	0.0
35	5.285167837111127E-4	0.0	0.0	17.233082508076397	0.0
36	5.285167837111127E-4	0.0	0.0	17.60515832380902	0.0
37	5.285167837111127E-4	0.0	0.0	17.972477488488245	0.0
38	5.285167837111127E-4	0.0	0.0	18.363712037630396	0.0
39	5.285167837111127E-4	0.0	0.0	18.766045439230478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	25	3.889539E-5	45.0	1
TAACCGA	20	7.031759E-4	45.0	40
CAATGCG	20	7.031759E-4	45.0	1
TAGGCGT	20	7.031759E-4	45.0	1
CGACTAA	25	3.889539E-5	45.0	15
CGTCCAA	20	7.031759E-4	45.0	14
CGAATAT	120	0.0	43.125004	14
AATGCGG	80	0.0	42.1875	2
TTGCGAG	70	0.0	41.785717	1
CGGTAGT	120	0.0	41.250004	12
CGACGGT	55	6.002665E-11	40.909092	28
TTACGGG	110	0.0	40.909092	3
TCGTCCC	90	0.0	40.0	38
TATAGCG	45	1.9268555E-8	40.0	1
TACGAAT	130	0.0	39.807693	12
GCTACGA	130	0.0	39.807693	10
TTAGCGG	75	0.0	39.000004	2
TACGGCT	625	0.0	38.879997	7
TCGCCTA	35	6.24546E-6	38.57143	31
ATAGCGG	105	0.0	38.57143	2
>>END_MODULE