##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935587.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606890
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.214122822916835	33.0	31.0	34.0	30.0	34.0
2	32.204570844798894	33.0	31.0	34.0	30.0	34.0
3	32.21812354792466	34.0	31.0	34.0	30.0	34.0
4	35.854128425250046	37.0	35.0	37.0	35.0	37.0
5	35.930292145199296	37.0	35.0	37.0	35.0	37.0
6	35.873703636573346	37.0	35.0	37.0	35.0	37.0
7	36.121158694326816	37.0	35.0	37.0	35.0	37.0
8	35.931257723804976	37.0	35.0	37.0	35.0	37.0
9	37.74293034981628	39.0	38.0	39.0	35.0	39.0
10	37.40064262057375	39.0	37.0	39.0	34.0	39.0
11	37.39595149038541	39.0	37.0	39.0	35.0	39.0
12	37.32945179521824	39.0	37.0	39.0	35.0	39.0
13	37.2926873074198	39.0	37.0	39.0	34.0	39.0
14	38.459539620029986	40.0	38.0	41.0	34.0	41.0
15	38.581540312082915	40.0	38.0	41.0	35.0	41.0
16	38.36322727347625	40.0	38.0	41.0	34.0	41.0
17	38.4240867373	40.0	38.0	41.0	34.0	41.0
18	38.34873700341083	40.0	37.0	41.0	34.0	41.0
19	38.23135329301851	40.0	37.0	41.0	34.0	41.0
20	38.38141837894841	40.0	37.0	41.0	34.0	41.0
21	38.31932640181911	40.0	37.0	41.0	34.0	41.0
22	38.42537527393762	40.0	37.0	41.0	34.0	41.0
23	38.442661767371355	40.0	37.0	41.0	34.0	41.0
24	38.37473182949134	40.0	37.0	41.0	34.0	41.0
25	38.10528761390038	40.0	37.0	41.0	34.0	41.0
26	38.19995056764817	40.0	37.0	41.0	34.0	41.0
27	38.20331361531744	40.0	37.0	41.0	34.0	41.0
28	38.080680189161136	40.0	37.0	41.0	34.0	41.0
29	38.02713177017252	40.0	37.0	41.0	34.0	41.0
30	37.88647036530508	40.0	36.0	41.0	34.0	41.0
31	37.87158957966024	40.0	36.0	41.0	34.0	41.0
32	37.880780701609844	40.0	36.0	41.0	34.0	41.0
33	37.83212938094218	40.0	36.0	41.0	34.0	41.0
34	37.76589497272982	40.0	36.0	41.0	33.0	41.0
35	37.70740167081349	40.0	36.0	41.0	33.0	41.0
36	37.58083342945179	40.0	36.0	41.0	33.0	41.0
37	37.55111469953369	40.0	36.0	41.0	33.0	41.0
38	37.44417439733724	40.0	36.0	41.0	33.0	41.0
39	37.375628202804464	40.0	36.0	41.0	33.0	41.0
40	37.2853910922902	40.0	35.0	41.0	33.0	41.0
41	37.13492230882038	40.0	35.0	41.0	32.0	41.0
42	37.13405394717329	40.0	35.0	41.0	32.0	41.0
43	37.0649310418692	40.0	35.0	41.0	32.0	41.0
44	36.96058264265353	39.0	35.0	41.0	32.0	41.0
45	36.968139201502744	39.0	35.0	41.0	32.0	41.0
46	36.829260656791185	39.0	35.0	41.0	32.0	41.0
47	36.762199080558254	39.0	35.0	41.0	31.0	41.0
48	36.66565275420587	39.0	35.0	41.0	31.0	41.0
49	36.58780339105934	39.0	35.0	41.0	31.0	41.0
50	36.479286196839624	39.0	35.0	41.0	31.0	41.0
51	35.328562012885364	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	12.0
10	16.0
11	18.0
12	17.0
13	16.0
14	22.0
15	28.0
16	45.0
17	75.0
18	125.0
19	238.0
20	409.0
21	677.0
22	1053.0
23	1496.0
24	2224.0
25	2903.0
26	3945.0
27	4693.0
28	5280.0
29	6200.0
30	7474.0
31	9447.0
32	12450.0
33	17353.0
34	33380.0
35	46970.0
36	40261.0
37	62681.0
38	116053.0
39	231170.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70413089686764	20.223269455749808	23.916690009721698	12.15590963766086
2	32.09444874688988	24.71551681523835	25.432780240241232	17.75725419763054
3	33.235017878033915	23.726210680683486	26.220402379343867	16.818369061938736
4	29.952380167740444	25.36093855558668	25.449587239862247	19.237094036810625
5	26.320585279045627	29.710820741814825	24.005503468503353	19.963090510636196
6	25.999934090197563	35.14607259964738	23.765591787638616	15.088401522516437
7	79.46448285521264	6.91492692250655	9.198701576892024	4.421888645388786
8	79.89223747301818	6.645355830545897	8.52411474896604	4.938291947469888
9	72.87795152334031	8.887772083903178	11.502249172008106	6.732027220748406
10	39.112854059219956	27.90456260607359	18.51571124915553	14.466872085550925
11	30.345202590255237	27.01741666529355	24.77994364711892	17.8574370973323
12	28.902437014945047	23.51282769529898	28.32078300845293	19.263952281303037
13	25.334080311094265	24.969928652638863	29.89273179653644	19.803259239730426
14	20.186854289904264	29.218474517622635	29.200843645471174	21.39382754700193
15	19.90953879615746	27.011155234062183	33.986389625797095	19.09291634398326
16	22.21143864621266	25.561469129496285	32.262024419581806	19.965067804709253
17	21.97564632800013	25.076372983571982	28.385209840333502	24.562770848094384
18	23.292853729670945	25.327983654368996	29.431692728501048	21.94746988745901
19	24.925109986982815	26.36606304272603	27.809982039578834	20.898844930712322
20	26.60465652754206	25.257624940269242	28.118110365964178	20.019608166224522
21	25.096640247820858	26.050519863566706	28.108882993623226	20.743956894989207
22	22.987691344395195	25.992024913905322	27.02977475325018	23.990508988449307
23	23.14224983110613	26.72230552488919	27.973767898630726	22.161676745373956
24	22.560431050107926	25.294534429633046	29.182059351777095	22.962975168481933
25	22.073192835604473	26.36589826821994	28.2515777158958	23.309331180279788
26	21.56024979815123	27.897477302311785	27.89681820428743	22.64545469524955
27	22.90678706190578	27.720674257278915	27.853812058198358	21.51872662261695
28	21.023084908302987	27.383216068809833	29.536983637891545	22.056715384995634
29	21.923248035064013	26.240504869086656	29.980062284763303	21.85618481108603
30	23.25396694623408	25.810278633689798	29.25192374235858	21.683830677717545
31	23.061015999604543	26.844897757418973	27.1930662887838	22.901019954192687
32	23.125113282472938	27.404801529107413	28.59249616899273	20.877589019426914
33	23.616965183146863	26.543525185783256	27.37019888282885	22.469310748241032
34	21.351480498937203	25.811926378750677	30.15521758473529	22.681375537576827
35	22.411969220122263	24.940928339567304	29.42114716011139	23.22595528019905
36	23.08622649903607	26.26505627049383	28.393283791131836	22.255433439338265
37	22.14470497124685	26.920529255713554	29.219298390153075	21.71546738288652
38	21.698001285241148	26.762016180856495	28.079717906045577	23.460264627856777
39	23.19777883965793	24.42040567483399	29.196559508312873	23.185255977195208
40	23.817990080574734	23.950468783469823	30.75845705152499	21.473084084430457
41	20.506846380727975	25.814397996342002	29.704559310583466	23.974196312346553
42	21.235808795663136	26.517655588327372	29.10741650051904	23.139119115490452
43	23.089851538170013	25.834500486084792	28.16573019822373	22.909917777521464
44	21.607869630410782	26.52638863715006	28.05022326945575	23.815518462983405
45	21.981248661207136	25.402956054639226	27.87819868509944	24.737596599054193
46	22.688625615844714	26.534627362454483	28.272504078169025	22.50424294353178
47	22.041885679447677	25.251363509037883	30.035756067821183	22.670994743693257
48	22.171068892220998	24.531298917431496	29.206775527690354	24.090856662657153
49	20.586597241674767	24.551071858162103	30.657615053798875	24.204715846364248
50	20.41902156898285	24.916212163654038	30.313895434098438	24.350870833264675
51	20.90823707755936	24.807131440623508	28.6556048048246	25.629026676992535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	323.0
1	782.0
2	1241.0
3	1896.0
4	2551.0
5	1843.0
6	1135.0
7	1103.5
8	1072.0
9	1104.5
10	1137.0
11	1214.5
12	1292.0
13	1259.5
14	1227.0
15	1310.5
16	1394.0
17	1393.5
18	1393.0
19	1587.0
20	1781.0
21	2217.5
22	2654.0
23	2847.0
24	3040.0
25	3811.0
26	5661.0
27	6740.0
28	7655.5
29	8571.0
30	10457.0
31	12343.0
32	13514.5
33	14686.0
34	16170.5
35	17655.0
36	18996.0
37	20337.0
38	21407.0
39	22477.0
40	26053.5
41	29630.0
42	32706.0
43	35782.0
44	38657.5
45	41533.0
46	45815.5
47	50098.0
48	52351.0
49	54604.0
50	52652.0
51	50700.0
52	45573.5
53	40447.0
54	36479.5
55	32512.0
56	30122.0
57	27732.0
58	27882.5
59	28033.0
60	26463.0
61	24893.0
62	23301.0
63	21709.0
64	18711.5
65	15714.0
66	12625.5
67	9537.0
68	8003.5
69	6470.0
70	5201.5
71	3933.0
72	3232.5
73	2532.0
74	2106.5
75	1369.0
76	1057.0
77	721.5
78	386.0
79	271.0
80	156.0
81	111.5
82	67.0
83	50.5
84	34.0
85	22.5
86	11.0
87	7.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.134337248052304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56338245895432	19.96093628748722
2	9.863871446239836	5.352992287874103
3	3.402621912055793	2.7698367146800336
4	1.6983985433972744	1.8433967543256973
5	1.0392533837174587	1.4099725899984514
6	0.7599367200554531	1.2372227549498027
7	0.5954881969724003	1.1310724294718595
8	0.5016376144934444	1.0889283366378855
9	0.4415378246543468	1.0782752617748188
>10	7.529666220041754	50.732499290252285
>50	0.5353103799484766	8.755210973065777
>100	0.062188677397203435	3.069998299897616
>500	0.004877543325270857	0.8511688986890787
>1k	0.0018290787469765716	0.7184891208953681
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1555	0.25622435696748996	TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1502	0.24749130814480386	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1286	0.21190001482970555	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	991	0.16329153553362225	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	672	0.11072846809141691	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	628	0.10347838982352651	TruSeq Adapter, Index 23 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	625	0.10298406630526126	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	624	0.10281929179917282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1693881922588937	0.0
2	0.0	0.0	0.0	0.8706684901712007	0.0
3	0.0	0.0	0.0	1.1662739540938225	0.0
4	0.0	0.0	0.0	1.53734614180494	0.0
5	0.0	0.0	0.0	2.5096805022326945	0.0
6	0.0	0.0	0.0	3.1430737036365732	0.0
7	0.0	0.0	0.0	3.667550956516008	0.0
8	0.0	0.0	0.0	4.428314851126234	0.0
9	0.0	0.0	0.0	4.693437031422499	0.0
10	0.0	0.0	0.0	5.4322859167229645	0.0
11	0.0	0.0	0.0	6.330142200398754	0.0
12	0.0	0.0	0.0	7.168514887376625	0.0
13	0.0	0.0	0.0	7.547331476873898	0.0
14	0.0	0.0	0.0	7.735339188320783	0.0
15	0.0	0.0	0.0	8.011995584043238	0.0
16	0.0	0.0	0.0	8.577501688938687	0.0
17	0.0	0.0	0.0	9.197712929855493	0.0
18	0.0	0.0	0.0	9.956499530392657	0.0
19	0.0	0.0	0.0	10.395623589118292	0.0
20	0.0	0.0	0.0	10.81876452075335	0.0
21	0.0	0.0	0.0	11.349503204864144	0.0
22	0.0	0.0	0.0	11.910560398095207	0.0
23	0.0	0.0	0.0	12.441134307699913	0.0
24	0.0	0.0	0.0	12.875974229267248	0.0
25	0.0	0.0	0.0	13.25808630888629	0.0
26	0.0	0.0	0.0	13.615811761604245	0.0
27	0.0	0.0	0.0	14.013577419301686	0.0
28	1.64774506088418E-4	0.0	0.0	14.397007694969433	0.0
29	1.64774506088418E-4	0.0	0.0	14.80482459753827	0.0
30	1.64774506088418E-4	0.0	0.0	15.263556822488425	0.0
31	3.29549012176836E-4	0.0	0.0	15.67961245036168	0.0
32	3.29549012176836E-4	0.0	0.0	16.09599762724711	0.0
33	3.29549012176836E-4	0.0	0.0	16.51254757863863	0.0
34	3.29549012176836E-4	0.0	0.0	16.933546441694542	0.0
35	3.29549012176836E-4	0.0	0.0	17.363278353573136	0.0
36	4.94323518265254E-4	0.0	0.0	17.787078383232547	0.0
37	4.94323518265254E-4	0.0	0.0	18.205935177709303	0.0
38	4.94323518265254E-4	0.0	0.0	18.60024057077889	0.0
39	4.94323518265254E-4	0.0	0.0	19.024370149450476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	30	2.1638007E-6	45.000004	1
CCCCGAT	30	2.1638007E-6	45.000004	40
CGTAAGG	35	1.2106648E-7	45.000004	2
CACAACG	45	3.8380676E-10	45.000004	12
TGCGTAG	45	3.8380676E-10	45.000004	1
CCCGATC	30	2.1638007E-6	45.000004	41
CGTCGAC	20	7.0306525E-4	45.0	16
CAGTCGA	25	3.8886217E-5	45.0	12
CGACTAG	25	3.8886217E-5	45.0	24
TCGACGG	50	2.1827873E-11	45.0	2
TATAACG	20	7.0306525E-4	45.0	18
CGATAGA	20	7.0306525E-4	45.0	10
TAATGCG	20	7.0306525E-4	45.0	1
ACGACTA	20	7.0306525E-4	45.0	23
TACCGGT	20	7.0306525E-4	45.0	40
ACACGAC	75	0.0	42.0	26
CACGACC	75	0.0	42.0	27
TTGTGCG	70	0.0	41.785717	1
GGCGATA	180	0.0	41.250004	8
ACGCCGG	95	0.0	40.263157	27
>>END_MODULE