##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686739
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1883102605211	33.0	31.0	34.0	30.0	34.0
2	32.19259136294866	34.0	31.0	34.0	30.0	34.0
3	32.18479364066989	34.0	31.0	34.0	30.0	34.0
4	35.84207100514169	37.0	35.0	37.0	35.0	37.0
5	35.90618561054491	37.0	35.0	37.0	35.0	37.0
6	35.84769468458905	37.0	35.0	37.0	35.0	37.0
7	36.085986087873266	37.0	35.0	37.0	35.0	37.0
8	35.91133603887358	37.0	35.0	37.0	35.0	37.0
9	37.735966648173466	39.0	38.0	39.0	35.0	39.0
10	37.373974683249386	39.0	37.0	39.0	34.0	39.0
11	37.361311939470454	39.0	37.0	39.0	35.0	39.0
12	37.20257623347443	39.0	37.0	39.0	34.0	39.0
13	37.1474257323379	39.0	37.0	39.0	34.0	39.0
14	38.19778984446784	40.0	37.0	41.0	33.0	41.0
15	38.34675327890218	40.0	38.0	41.0	34.0	41.0
16	38.180396045659265	40.0	37.0	41.0	33.0	41.0
17	38.22869532675441	40.0	37.0	41.0	34.0	41.0
18	38.13440768618063	40.0	37.0	41.0	34.0	41.0
19	38.00394909856583	40.0	37.0	41.0	34.0	41.0
20	38.118111829967425	40.0	37.0	41.0	34.0	41.0
21	37.99777207934892	40.0	36.0	41.0	34.0	41.0
22	38.125469792745136	40.0	36.0	41.0	34.0	41.0
23	38.158119751463076	40.0	36.0	41.0	34.0	41.0
24	38.1064305362008	40.0	36.0	41.0	34.0	41.0
25	37.823643334658435	40.0	36.0	41.0	33.0	41.0
26	37.9115690240397	40.0	36.0	41.0	34.0	41.0
27	37.910265763266686	40.0	36.0	41.0	34.0	41.0
28	37.760885867847904	40.0	36.0	41.0	33.0	41.0
29	37.66675403610397	40.0	36.0	41.0	33.0	41.0
30	37.504640045199125	40.0	35.0	41.0	33.0	41.0
31	37.48484504302217	40.0	35.0	41.0	33.0	41.0
32	37.43332328584805	40.0	35.0	41.0	33.0	41.0
33	37.364971262735914	40.0	35.0	41.0	33.0	41.0
34	37.21764309293633	40.0	35.0	41.0	32.0	41.0
35	37.12828163246881	40.0	35.0	41.0	32.0	41.0
36	37.03410611600623	40.0	35.0	41.0	32.0	41.0
37	37.005625135604646	40.0	35.0	41.0	32.0	41.0
38	36.852089367285096	39.0	35.0	41.0	31.0	41.0
39	36.764754877762876	39.0	35.0	41.0	31.0	41.0
40	36.66895720208114	39.0	35.0	41.0	31.0	41.0
41	36.518220168069675	39.0	35.0	41.0	31.0	41.0
42	36.52451076755507	39.0	35.0	41.0	31.0	41.0
43	36.43982794045482	39.0	35.0	41.0	31.0	41.0
44	36.3143683408107	39.0	35.0	41.0	30.0	41.0
45	36.294596637150356	39.0	35.0	41.0	30.0	41.0
46	36.17808803635734	38.0	35.0	41.0	30.0	41.0
47	36.1210736538918	38.0	35.0	41.0	30.0	41.0
48	35.98494187748184	38.0	35.0	40.0	30.0	41.0
49	35.87943745731639	38.0	35.0	40.0	30.0	41.0
50	35.70460247634109	38.0	35.0	40.0	29.0	41.0
51	34.57704164172997	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	15.0
10	27.0
11	24.0
12	18.0
13	22.0
14	31.0
15	43.0
16	89.0
17	118.0
18	241.0
19	368.0
20	633.0
21	1069.0
22	1633.0
23	2310.0
24	3315.0
25	4560.0
26	5838.0
27	6810.0
28	7480.0
29	8513.0
30	9985.0
31	12437.0
32	16045.0
33	23044.0
34	44785.0
35	54761.0
36	48346.0
37	72879.0
38	127223.0
39	233937.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30416795900626	19.954305784293595	23.065968293631204	12.67555796306894
2	33.00933833668977	24.74899488743176	23.758662315668687	18.483004460209774
3	32.515118553045625	24.569013846599656	25.624582264877922	17.291285335476797
4	29.33268679949734	25.182929759340883	26.043227485260047	19.44115595590173
5	26.224082220465128	28.903411630910725	24.751033507635363	20.121472640988788
6	26.101182545333817	34.64081696248502	24.337630453491062	14.920370038690098
7	79.24888494755649	6.580083554305202	9.490650742130562	4.680380756007741
8	80.28464962671407	6.6074593113249716	8.720052305169796	4.387838756791154
9	74.07384756071812	8.42124882961358	10.655722188487912	6.849181421180391
10	41.236918246961366	26.135693473066183	17.63304545103744	14.99434282893501
11	33.00132947160421	25.873585161174766	23.47150809841876	17.653577268802266
12	29.651148398445404	23.226291211071455	27.75566845628397	19.366891934199167
13	27.483512659103386	24.43446491316206	28.515345713582597	19.566676714151956
14	21.729361518713805	27.521809595785296	29.30997074580008	21.438858139700816
15	20.643941876025682	26.084145505060874	33.352408993809874	19.91950362510357
16	25.033091174376292	25.03527541030872	30.15352266290396	19.77811075241103
17	25.086677762585204	24.33996030515232	27.862987248430628	22.710374683831848
18	26.750483080180388	24.65783944118508	27.98690623366373	20.604771244970795
19	27.186310956564284	26.376541888548633	25.778789321707375	20.658357833179707
20	28.049957844246503	26.26645639755424	26.34625381695229	19.337331941246966
21	26.56875465060234	26.766355194622705	27.72363299594169	18.94125715883327
22	24.98751345125295	24.872040178291897	27.93754250159085	22.202903868864297
23	24.209197380664268	26.164671003103074	28.310901230307294	21.315230385925364
24	23.714395134104805	25.25937801697588	28.593250128505883	22.432976720413432
25	24.14454399706439	26.464785020218745	26.772179823775843	22.618491158941023
26	23.34962773338925	26.66806457766342	26.73417411855159	23.24813357039574
27	23.352103200779336	26.57894775162034	27.76251239553892	22.30643665206141
28	22.037921248101537	28.087235470826617	27.969577961933133	21.90526531913871
29	23.883891842461257	26.92056225145215	27.699752016413804	21.495793889672786
30	24.597700145178884	25.493382493203388	28.064082569942876	21.84483479167486
31	25.00542418589886	26.64928014864454	26.572395043823054	21.772900621633546
32	25.601283748265352	26.90381644263687	27.14859648279769	20.346303326300095
33	25.89600998341437	26.20748202737867	26.30198663538841	21.594521353818553
34	23.830887717167656	27.094281815944633	28.481126017307883	20.593704449579825
35	22.921808722090926	27.01928971559792	28.1631012655463	21.895800296764854
36	24.746373804312846	28.524519504498798	26.007114784510566	20.721991906677793
37	23.8081716634704	28.4399167660494	26.41527567241703	21.336635898063168
38	23.999365115422307	27.698441474854345	26.40959665899272	21.89259675073063
39	24.542511783952854	25.650065017422925	26.966285590304324	22.8411376083199
40	24.823841372049642	25.237535657651595	29.06679247865637	20.871830491642385
41	22.234356866291268	25.547697160056444	28.339150681700037	23.878795291952258
42	24.01960570172948	25.921201504501713	27.731933092484915	22.327259701283893
43	23.511698039575442	26.118510817064415	27.40327839251885	22.966512750841297
44	23.161200980285088	26.295433927591123	27.10403806977614	23.439327022347644
45	22.802549440180332	25.50736160317093	26.660929406950824	25.029159549697923
46	22.639605439621167	27.118162795472518	26.477744820084485	23.764486944821833
47	22.25255883239484	26.60821651311488	28.542867086331196	22.596357568159082
48	22.779979002211903	26.1913186814787	28.4817084802232	22.546993836086198
49	24.069115049531188	25.7921859687596	27.923272160165652	22.215426821543556
50	21.00026356446918	26.666025957459823	29.224057465791226	23.109653012279775
51	21.840320704081172	26.177630802968814	27.209609473176855	24.77243901977316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	856.0
2	1420.0
3	2830.5
4	4241.0
5	2950.5
6	1660.0
7	1633.0
8	1606.0
9	1633.5
10	1661.0
11	1692.5
12	1724.0
13	1738.0
14	1752.0
15	1634.5
16	1517.0
17	1625.5
18	1734.0
19	1916.0
20	2098.0
21	2399.5
22	2701.0
23	3054.0
24	3407.0
25	4039.5
26	5511.5
27	6351.0
28	6999.5
29	7648.0
30	9195.5
31	10743.0
32	12808.5
33	14874.0
34	17216.5
35	19559.0
36	20322.0
37	21085.0
38	22788.0
39	24491.0
40	27121.0
41	29751.0
42	32920.0
43	36089.0
44	38371.0
45	40653.0
46	46736.0
47	52819.0
48	52690.5
49	52562.0
50	52971.5
51	53381.0
52	50669.5
53	47958.0
54	44778.5
55	41599.0
56	40883.5
57	40168.0
58	38723.0
59	37278.0
60	35198.0
61	33118.0
62	30764.0
63	28410.0
64	24209.0
65	20008.0
66	16628.0
67	13248.0
68	11228.5
69	9209.0
70	7695.5
71	6182.0
72	5099.0
73	4016.0
74	3181.5
75	1923.5
76	1500.0
77	1039.0
78	578.0
79	433.0
80	288.0
81	253.5
82	219.0
83	129.5
84	40.0
85	31.0
86	22.0
87	19.5
88	17.0
89	25.0
90	33.0
91	19.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.631647311577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89956180254322	20.419666282079614
2	8.098378897904519	4.475430990048306
3	2.9733511008757114	2.4647576685866057
4	1.6506795985012115	1.8244398596080398
5	1.1635411264486761	1.6075279019272415
6	0.8749204440248209	1.4505295880989317
7	0.7551855874654485	1.4606915265341949
8	0.659572932623039	1.458006932040188
9	0.5884599271378432	1.4634105447302264
>10	9.063054926965092	56.338690523237055
>50	0.2266854351908335	3.7819235644187197
>100	0.041311831646912656	1.8859859521185989
>500	0.0031778332036086652	0.5188034608722305
>1k	0.002118555469072444	0.8501352057000808
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2295	0.33418809766155705	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	1264	0.18405828123930634	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	1132	0.16483700503393572	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	1118	0.16279838483033582	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	829	0.12071543919888052	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.824629153142605E-4	0.0	0.0	0.07135170712599692	0.0
2	5.824629153142605E-4	0.0	0.0	0.3628743962407843	0.0
3	5.824629153142605E-4	0.0	0.0	0.540816816869291	0.0
4	5.824629153142605E-4	0.0	0.0	0.776859913300395	0.0
5	5.824629153142605E-4	0.0	0.0	1.4381009379109093	0.0
6	5.824629153142605E-4	0.0	0.0	1.9366891934199164	0.0
7	5.824629153142605E-4	0.0	0.0	2.3043689087120436	0.0
8	5.824629153142605E-4	0.0	0.0	2.875182565720019	0.0
9	5.824629153142605E-4	0.0	0.0	3.1187976800502084	0.0
10	5.824629153142605E-4	0.0	0.0	3.7021342897374403	0.0
11	7.280786441428257E-4	0.0	0.0	4.302071092511128	0.0
12	7.280786441428257E-4	0.0	0.0	4.924432717524416	0.0
13	7.280786441428257E-4	0.0	0.0	5.202849991044633	0.0
14	7.280786441428257E-4	0.0	0.0	5.350941187263284	0.0
15	7.280786441428257E-4	0.0	0.0	5.528301144976476	0.0
16	7.280786441428257E-4	0.0	0.0	5.8308906294822345	0.0
17	7.280786441428257E-4	0.0	0.0	6.182843846060876	0.0
18	7.280786441428257E-4	0.0	0.0	6.5811028644070015	0.0
19	7.280786441428257E-4	0.0	0.0	6.834183001111048	0.0
20	7.280786441428257E-4	0.0	0.0	7.096873775917779	0.0
21	7.280786441428257E-4	0.0	0.0	7.410675671543338	0.0
22	7.280786441428257E-4	0.0	0.0	7.757095490426494	0.0
23	7.280786441428257E-4	0.0	0.0	8.070023691679081	0.0
24	7.280786441428257E-4	0.0	0.0	8.330675846282212	0.0
25	7.280786441428257E-4	0.0	0.0	8.5936578525466	0.0
26	7.280786441428257E-4	0.0	0.0	8.847029220708304	0.0
27	7.280786441428257E-4	0.0	0.0	9.138260678365434	0.0
28	7.280786441428257E-4	0.0	0.0	9.397311059951452	0.0
29	7.280786441428257E-4	0.0	0.0	9.673107250352754	0.0
30	0.001019310101799956	0.0	0.0	10.033360563474623	0.0
31	0.001019310101799956	0.0	0.0	10.328814877267783	0.0
32	0.001019310101799956	0.0	0.0	10.626307811264542	0.0
33	0.001019310101799956	0.0	0.0	10.93996409116127	0.0
34	0.001019310101799956	0.0	0.0	11.248815052006657	0.0
35	0.001019310101799956	0.0	0.0	11.56625734085293	0.0
36	0.001019310101799956	0.0	0.0	11.871759139935259	0.0
37	0.001164925830628521	0.0	0.0	12.175367934542818	0.0
38	0.0014561572882856515	0.0	0.0	12.52630184101966	0.0
39	0.0014561572882856515	0.0	0.0	13.020812856121466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCA	20	7.0313E-4	45.000004	44
CCGGCAT	20	7.0313E-4	45.000004	33
ATCTCGA	30	2.1642209E-6	44.999996	28
ACGGGTA	150	0.0	42.0	5
TACGGGT	125	0.0	41.399998	4
AACACGT	165	0.0	40.90909	41
ATAGCGG	50	1.0804797E-9	40.500004	2
GCGAGAC	190	0.0	40.263157	21
CACGACC	180	0.0	40.0	27
GCGTTAG	40	3.455516E-7	39.375004	1
GTTCGCC	40	3.455516E-7	39.375004	22
CTACGAA	75	0.0	39.0	11
TACGAAT	75	0.0	39.0	12
GCGATCA	70	0.0	38.571426	9
TCACGAC	30	1.13944334E-4	37.499996	25
ATTACGG	30	1.13944334E-4	37.499996	2
CGATGAC	30	1.13944334E-4	37.499996	10
CGGTCTA	30	1.13944334E-4	37.499996	31
TAGTGCG	30	1.13944334E-4	37.499996	1
TACGTAG	30	1.13944334E-4	37.499996	1
>>END_MODULE