##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662953
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03962271835258	33.0	31.0	34.0	30.0	34.0
2	32.0667121198637	33.0	31.0	34.0	30.0	34.0
3	32.070546479162175	33.0	31.0	34.0	30.0	34.0
4	35.73651978345373	37.0	35.0	37.0	35.0	37.0
5	35.79226732513466	37.0	35.0	37.0	35.0	37.0
6	35.73006532891472	37.0	35.0	37.0	35.0	37.0
7	35.990984277920155	37.0	35.0	37.0	35.0	37.0
8	35.75181347697348	37.0	35.0	37.0	35.0	37.0
9	37.57827628806265	39.0	37.0	39.0	35.0	39.0
10	37.187477845337455	39.0	37.0	39.0	34.0	39.0
11	37.19830666729014	39.0	37.0	39.0	34.0	39.0
12	37.13623439369005	39.0	37.0	39.0	34.0	39.0
13	37.054211987878475	39.0	37.0	39.0	33.0	39.0
14	38.20836922074415	40.0	37.0	41.0	33.0	41.0
15	38.23274048084857	40.0	37.0	41.0	33.0	41.0
16	38.138344648866514	40.0	37.0	41.0	33.0	41.0
17	38.07951845756788	40.0	37.0	41.0	33.0	41.0
18	38.019156712466796	40.0	37.0	41.0	33.0	41.0
19	37.87225187909249	40.0	37.0	41.0	33.0	41.0
20	37.88480933037485	40.0	36.0	41.0	33.0	41.0
21	37.96736118548374	40.0	36.0	41.0	33.0	41.0
22	38.03044258039409	40.0	37.0	41.0	34.0	41.0
23	38.02880898042546	40.0	37.0	41.0	34.0	41.0
24	37.95984632394755	40.0	36.0	41.0	33.0	41.0
25	37.804527621113415	40.0	36.0	41.0	33.0	41.0
26	37.81467917031826	40.0	36.0	41.0	33.0	41.0
27	37.8036346468	40.0	36.0	41.0	33.0	41.0
28	37.68818000672748	40.0	36.0	41.0	33.0	41.0
29	37.69001573263866	40.0	36.0	41.0	33.0	41.0
30	37.55890236562773	40.0	36.0	41.0	33.0	41.0
31	37.53747249050838	40.0	36.0	41.0	33.0	41.0
32	37.434009650759556	40.0	36.0	41.0	33.0	41.0
33	37.334391729127105	40.0	36.0	41.0	33.0	41.0
34	37.21147200480275	40.0	36.0	41.0	32.0	41.0
35	37.104372406490356	40.0	35.0	41.0	32.0	41.0
36	37.057806511170476	40.0	35.0	41.0	32.0	41.0
37	37.004614203420154	40.0	35.0	41.0	32.0	41.0
38	36.89239357842864	40.0	35.0	41.0	31.0	41.0
39	36.818739790000194	40.0	35.0	41.0	31.0	41.0
40	36.73522406565775	39.0	35.0	41.0	31.0	41.0
41	36.54776281274842	39.0	35.0	41.0	30.0	41.0
42	36.67058750771171	39.0	35.0	41.0	31.0	41.0
43	36.654138377833725	39.0	35.0	41.0	31.0	41.0
44	36.61875577906729	39.0	35.0	41.0	31.0	41.0
45	36.64026861632725	39.0	35.0	41.0	31.0	41.0
46	36.504889486886704	39.0	35.0	41.0	31.0	41.0
47	36.359331656995295	39.0	35.0	41.0	30.0	41.0
48	36.310326674741646	39.0	35.0	41.0	30.0	41.0
49	36.277259473899356	39.0	35.0	41.0	30.0	41.0
50	36.164317832485864	39.0	35.0	41.0	30.0	41.0
51	35.06352486526194	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	20.0
11	10.0
12	16.0
13	9.0
14	20.0
15	30.0
16	58.0
17	80.0
18	178.0
19	388.0
20	812.0
21	1527.0
22	2310.0
23	3000.0
24	3766.0
25	4715.0
26	5659.0
27	6275.0
28	6824.0
29	7465.0
30	9203.0
31	11746.0
32	15699.0
33	21795.0
34	38832.0
35	52345.0
36	46038.0
37	67383.0
38	123075.0
39	233526.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13343630694785	18.849450866049327	23.07539146817346	11.941721358829358
2	30.127324259789155	29.98357349616036	23.301953532150847	16.587148711899637
3	29.75354210630316	28.96947445746531	25.893238283860242	15.383745152371283
4	25.823248405241394	23.240410707848067	33.20506883595066	17.731272050959873
5	29.832431560005006	26.43671572494581	24.449546197090896	19.281306517958285
6	23.54812482936196	37.95910117308467	24.22268245260222	14.27009154495115
7	75.04815575161436	5.549865525912093	14.867418957301648	4.53455976517189
8	75.29855057598351	10.496068348736637	9.399459690204283	4.805921385075564
9	70.4105720918376	6.84135979473658	10.42969863625325	12.31836947717259
10	40.71887449034849	23.84829693809365	19.651015984541893	15.781812587015972
11	32.175885771691206	23.8940015355538	24.37925463796076	19.550858054794233
12	26.790436124431142	20.860000633529076	29.063447936731563	23.28611530530822
13	24.915190066264124	23.16695150334941	32.52085743634918	19.397000994037285
14	20.42165885062742	29.32590998155224	28.374107968438185	21.87832319938216
15	18.61866527491391	25.19288697690485	35.58125538311162	20.607192365069622
16	20.957895959442073	24.36960086159954	30.115558719848917	24.55694445910947
17	21.698672454910074	25.662000171957892	29.824286186200226	22.815041186931804
18	22.028107573236717	25.415074673468556	29.34370913171824	23.21310862157649
19	22.43794054782164	27.25049890414554	27.19091700316614	23.120643544866677
20	25.272681472140558	25.784482459540875	29.999260882747343	18.943575185571223
21	23.39230684528164	29.189399550194356	27.950246850078365	19.468046754445638
22	21.546022116198284	23.768200762346652	30.36474682217292	24.32103029928215
23	22.09251636239673	28.440326840665932	28.346202521144033	21.12095427579331
24	22.374285959939844	25.120785334706987	28.312414303879763	24.19251440147341
25	20.833603588791362	29.92610335875997	26.97234947273789	22.267943579710778
26	19.85992973860892	26.41469304762178	29.915695381120532	23.80968183264877
27	22.629960193256533	27.14129055905924	27.864569584872534	22.364179662811694
28	18.835573562530072	26.671272322472333	32.14179587391565	22.351358241081947
29	22.80780085466089	23.604388244717196	29.477202757963234	24.11060814265868
30	20.996058544120018	26.588611862379384	31.28638078415815	21.128948809342443
31	22.115594921510272	25.005995900161853	28.364906712843897	24.513502465483977
32	26.186773421343595	26.285422948534816	26.289193954925917	21.238609675195676
33	24.637342315367754	25.12380213981987	26.99708727466351	23.241768270148864
34	22.255423838492323	24.27638158361151	29.171449559772718	24.296745018123456
35	21.260934033031	24.05524976883731	28.88817155967316	25.79564463845853
36	21.115825707101408	27.30344383387661	28.425544495612808	23.15518596340917
37	22.024487407101255	26.091593220032188	31.096171221790986	20.787748151075565
38	22.345777151623118	26.514700137113795	27.39424966777434	23.745273043488755
39	24.487407101257556	25.051549657366362	28.379236537130083	22.081806704246002
40	23.037379723751155	23.421419014620945	29.407062039088743	24.134139222539154
41	19.243143933280336	27.407071089504083	29.02332442873024	24.326460548485336
42	20.390133237197812	25.265290299614	29.381268355373606	24.96330810781458
43	22.027051691447205	26.552561041280455	27.27976191374049	24.14062535353185
44	22.23868057011583	24.56071546550057	28.910345077252835	24.29025888713076
45	21.231067662413476	23.720987762330058	28.369130240001933	26.678814335254536
46	24.593296960719687	26.086766331851578	26.909901606901244	22.41003510052749
47	20.3052101732702	24.73357839846867	32.85044339493147	22.110768033329663
48	21.661565752021637	25.09423744971363	28.487087319915588	24.757109478349143
49	20.751999010487925	23.12984480046097	31.993821583128817	24.12433460592229
50	21.254749582549593	23.590058420431014	29.959891576024244	25.195300420995153
51	21.191698355690374	23.317791758993472	27.99776153060624	27.49274835470991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	187.0
1	674.5
2	1162.0
3	2647.0
4	4132.0
5	2811.0
6	1490.0
7	1474.0
8	1458.0
9	1469.0
10	1480.0
11	1491.0
12	1502.0
13	1491.0
14	1480.0
15	1472.0
16	1464.0
17	1575.0
18	1686.0
19	1831.5
20	1977.0
21	2004.0
22	2031.0
23	2385.0
24	2739.0
25	3205.0
26	4311.0
27	4951.0
28	5933.5
29	6916.0
30	8696.5
31	10477.0
32	12196.0
33	13915.0
34	15156.5
35	16398.0
36	18342.0
37	20286.0
38	21960.0
39	23634.0
40	26029.5
41	28425.0
42	32174.5
43	35924.0
44	38999.0
45	42074.0
46	65427.5
47	88781.0
48	75624.5
49	62468.0
50	61116.5
51	59765.0
52	52702.5
53	45640.0
54	41578.0
55	37516.0
56	34402.5
57	31289.0
58	29651.5
59	28014.0
60	26539.5
61	25065.0
62	22597.5
63	20130.0
64	17091.5
65	14053.0
66	11060.5
67	8068.0
68	6698.5
69	5329.0
70	4216.5
71	3104.0
72	2504.5
73	1905.0
74	1577.0
75	947.5
76	646.0
77	441.0
78	236.0
79	163.5
80	91.0
81	101.5
82	112.0
83	61.5
84	11.0
85	10.5
86	10.0
87	7.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	662953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.66630482880538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62725902061206	22.139150146436933
2	9.379648192147922	5.56519004910427
3	3.3116326967246827	2.9473171518621966
4	1.7781314522471132	2.11002358752197
5	1.14851903604535	1.7036157912503533
6	0.875501730097804	1.5583740721936765
7	0.664388495523242	1.3796966143060727
8	0.5875093731975262	1.3943385724046553
9	0.4954510401937312	1.3228381427522342
>10	6.827786972943993	46.09327650092476
>50	0.2525394339356735	4.528511886576681
>100	0.0444755683246724	2.252123292552675
>500	0.0015336402870576693	0.3694392148591459
>1k	0.004089707432153784	2.1844837647090056
>5k	5.11213429019223E-4	1.3744776700609356
>10k+	0.001022426858038446	3.0771435424844293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	10210	1.5400790101259063	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	10080	1.5204697768921778	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	9063	1.3670652369021634	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2750	0.41481070302117945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2229	0.33622292983062146	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT	1956	0.29504354003979166	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGGTCAT	1883	0.2840322013777749	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	1869	0.281920437798758	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC	1409	0.21253392020248793	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTA	1303	0.19654485310421707	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1005	0.1515944569222856	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT	946	0.14269488183928572	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	856	0.1291192588313199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.050415338643916E-4	0.0	0.0	0.1359070703353028	0.0
2	9.050415338643916E-4	0.0	0.0	0.8514932431107485	0.0
3	9.050415338643916E-4	0.0	0.0	1.109731760773388	0.0
4	9.050415338643916E-4	0.0	0.0	1.694086911138497	0.0
5	9.050415338643916E-4	0.0	0.0	3.8056996498997666	0.0
6	9.050415338643916E-4	0.0	0.0	4.5301853977582125	0.0
7	9.050415338643916E-4	0.0	0.0	5.289364404414792	0.0
8	9.050415338643916E-4	0.0	0.0	6.192445014955811	0.0
9	9.050415338643916E-4	0.0	0.0	6.47662805658923	0.0
10	9.050415338643916E-4	0.0	0.0	8.652951265021803	0.0
11	9.050415338643916E-4	0.0	0.0	9.609429326060821	0.0
12	9.050415338643916E-4	0.0	0.0	11.904614655940918	0.0
13	9.050415338643916E-4	0.0	0.0	12.29755352189371	0.0
14	9.050415338643916E-4	0.0	0.0	12.480371911734316	0.0
15	9.050415338643916E-4	0.0	0.0	12.918412014124682	0.0
16	9.050415338643916E-4	0.0	0.0	13.431268883314504	0.0
17	9.050415338643916E-4	0.0	0.0	14.028747135920646	0.0
18	9.050415338643916E-4	0.0	0.0	14.7186904652366	0.0
19	9.050415338643916E-4	0.0	0.0	15.45222662843369	0.0
20	9.050415338643916E-4	0.0	0.0	15.907311679711835	0.0
21	9.050415338643916E-4	0.0	0.0	16.42650383963871	0.0
22	9.050415338643916E-4	0.0	0.0	17.064708961268746	0.0
23	9.050415338643916E-4	0.0	0.0	17.6173876579486	0.0
24	9.050415338643916E-4	0.0	0.0	18.066137418489696	0.0
25	9.050415338643916E-4	0.0	0.0	18.476422913841553	0.0
26	0.001055881789508457	0.0	0.0	18.862423128034717	0.0
27	0.001055881789508457	0.0	0.0	19.302725834259743	0.0
28	0.001206722045152522	0.0	0.0	19.692949575610943	0.0
29	0.001206722045152522	0.0	0.0	20.107156917609544	0.0
30	0.001206722045152522	0.0	0.0	20.590298256437485	0.0
31	0.001206722045152522	0.0	0.0	21.031506004196377	0.0
32	0.001206722045152522	0.0	0.0	21.47542887655686	0.0
33	0.001206722045152522	0.0	0.0	21.888580336765955	0.0
34	0.001206722045152522	0.0	0.0	22.292681381636406	0.0
35	0.001206722045152522	0.0	0.0	22.71141393130433	0.0
36	0.001206722045152522	0.0	0.0	23.124565391513425	0.0
37	0.001206722045152522	0.0	0.0	23.53967777504589	0.0
38	0.001206722045152522	0.0	0.0	23.96067292854848	1.5084025564406526E-4
39	0.001206722045152522	0.0	0.0	24.381668082051064	1.5084025564406526E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	65	0.0	45.000004	2
AAGTACG	20	7.031125E-4	45.0	1
GCAACCG	20	7.031125E-4	45.0	12
GGCGTGA	25	3.889014E-5	45.0	8
TAGCGCG	20	7.031125E-4	45.0	1
TTCGTAG	35	1.210883E-7	45.0	1
TCGATTA	25	3.889014E-5	45.0	41
GCTACGA	35	1.210883E-7	45.0	10
CGGTATA	20	7.031125E-4	45.0	26
ATCGACG	20	7.031125E-4	45.0	1
GACTACG	20	7.031125E-4	45.0	12
CGTTCGG	30	2.1641063E-6	44.999996	2
CCGATGA	670	0.0	43.320896	18
CGATGAA	715	0.0	41.853146	19
CGTTTTT	1380	0.0	40.923912	1
CGTTAGG	55	6.002665E-11	40.909092	2
GATGAAT	755	0.0	40.5298	20
CACCGAG	125	0.0	39.6	16
TTGTGCG	40	3.4552613E-7	39.375	1
GCGTTAG	40	3.4552613E-7	39.375	1
>>END_MODULE