##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840697
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05679929867717	33.0	31.0	34.0	30.0	34.0
2	32.06816367847156	33.0	31.0	34.0	30.0	34.0
3	32.07482006002162	33.0	31.0	34.0	30.0	34.0
4	35.75459767312123	37.0	35.0	37.0	33.0	37.0
5	35.824099526940145	37.0	35.0	37.0	35.0	37.0
6	35.76733353396051	37.0	35.0	37.0	35.0	37.0
7	36.05625689160304	37.0	35.0	37.0	35.0	37.0
8	35.8791312446696	37.0	35.0	37.0	35.0	37.0
9	37.71236961711532	39.0	38.0	39.0	35.0	39.0
10	37.276634744741564	39.0	37.0	39.0	34.0	39.0
11	37.24881021343005	39.0	37.0	39.0	34.0	39.0
12	37.241034522544986	39.0	37.0	39.0	34.0	39.0
13	37.21502515174908	39.0	37.0	39.0	34.0	39.0
14	38.36838956247019	40.0	38.0	41.0	34.0	41.0
15	38.45120180041085	40.0	38.0	41.0	34.0	41.0
16	38.25835586424122	40.0	37.0	41.0	34.0	41.0
17	38.295045658542854	40.0	37.0	41.0	34.0	41.0
18	38.22899927084312	40.0	37.0	41.0	34.0	41.0
19	38.10061651225114	40.0	37.0	41.0	34.0	41.0
20	38.23000914717193	40.0	37.0	41.0	34.0	41.0
21	38.19763244070099	40.0	37.0	41.0	34.0	41.0
22	38.293329225630636	40.0	37.0	41.0	34.0	41.0
23	38.31076475828985	40.0	37.0	41.0	34.0	41.0
24	38.228760183514396	40.0	37.0	41.0	34.0	41.0
25	37.99434754733275	40.0	37.0	41.0	34.0	41.0
26	38.09319291016859	40.0	37.0	41.0	34.0	41.0
27	38.08577168706442	40.0	37.0	41.0	34.0	41.0
28	37.92509072828855	40.0	37.0	41.0	33.0	41.0
29	37.90200155347289	40.0	37.0	41.0	33.0	41.0
30	37.772417410791284	40.0	36.0	41.0	33.0	41.0
31	37.76485107000501	40.0	36.0	41.0	33.0	41.0
32	37.740511742042614	40.0	37.0	41.0	33.0	41.0
33	37.63951578273742	40.0	36.0	41.0	33.0	41.0
34	37.60314001358397	40.0	36.0	41.0	33.0	41.0
35	37.53105102076015	40.0	36.0	41.0	33.0	41.0
36	37.46383179671154	40.0	36.0	41.0	33.0	41.0
37	37.481913222005076	40.0	36.0	41.0	33.0	41.0
38	37.32139641273848	40.0	36.0	41.0	32.0	41.0
39	37.28597818238914	40.0	36.0	41.0	32.0	41.0
40	37.19704364354815	40.0	36.0	41.0	32.0	41.0
41	37.05861802766038	40.0	35.0	41.0	31.0	41.0
42	37.12122084413291	40.0	35.0	41.0	32.0	41.0
43	37.06396002364705	40.0	35.0	41.0	32.0	41.0
44	36.99709764635772	39.0	35.0	41.0	31.0	41.0
45	36.98196139631758	39.0	35.0	41.0	31.0	41.0
46	36.86861616016234	39.0	35.0	41.0	31.0	41.0
47	36.788595653368574	39.0	35.0	41.0	31.0	41.0
48	36.712115066427025	39.0	35.0	41.0	31.0	41.0
49	36.67754137340802	39.0	35.0	41.0	31.0	41.0
50	36.53870419425786	39.0	35.0	41.0	31.0	41.0
51	35.40817916562091	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	10.0
10	16.0
11	17.0
12	10.0
13	14.0
14	14.0
15	23.0
16	43.0
17	75.0
18	164.0
19	303.0
20	644.0
21	1098.0
22	1672.0
23	2463.0
24	3450.0
25	4715.0
26	6038.0
27	6958.0
28	7857.0
29	9019.0
30	10944.0
31	14368.0
32	19094.0
33	25585.0
34	44800.0
35	61852.0
36	56549.0
37	85818.0
38	165492.0
39	311362.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20034090760405	20.779781538413957	24.830943847783445	12.188933706198547
2	30.071476405887022	27.017700788750286	25.733647199882952	17.17717560547974
3	30.40167860715573	25.899699891875432	27.551900387416634	16.146721113552207
4	27.500514454077983	24.464105379227	29.398701315694005	18.636678851001015
5	26.00413704342944	29.006764625067056	25.682023368704776	19.307074962798726
6	24.048735751406277	35.43369370891058	25.576991472551942	14.940579067131202
7	79.20356561281888	4.991810366874153	11.274692308881797	4.529931711425163
8	80.32406443700881	6.793529654560442	8.160014844825186	4.7223910636055555
9	74.36044139565146	7.470824803704545	9.77189165656592	8.39684214407807
10	36.88606001924594	32.35125140211039	17.397825851644527	13.364862726999144
11	26.818223450303734	26.296632437132523	28.56724836653396	18.317895746029784
12	25.457090961428435	22.177788192416532	31.76340584062986	20.60171500552518
13	23.309706112903935	24.471718110091984	33.68181401860599	18.53676175839809
14	19.691874718239745	29.70594637544799	28.999627689881137	21.602551216431127
15	17.86327297468648	27.058024472550752	35.36934234331751	19.709360209445258
16	20.67510648902042	27.127133794934444	30.397515394963943	21.8002443210812
17	21.56151383911207	25.94537627706534	29.08955307322377	23.403556810598825
18	21.515480607162864	26.496109775579075	29.891149843522697	22.097259773735363
19	21.94583779887403	27.755540938054974	28.300921735179262	21.997699527891736
20	23.63848092713546	26.803354835333064	30.11453591484209	19.44362832268939
21	23.104043430629584	28.01366009394586	29.633506483310875	19.24878999211369
22	21.37773775807455	25.087992463396443	29.40774143359617	24.126528344932836
23	20.902061027932774	26.587581494878656	29.77196302591778	22.73839445127079
24	21.6889081321808	25.14996485059421	29.360280814609784	23.80084620261521
25	20.05157625161027	29.006288829387994	28.24679997668601	22.69533494231572
26	20.05978372707408	27.310077233533605	28.46102698118347	24.169112058208846
27	21.679630116439096	27.784564474477726	27.768268472469863	22.76753693661331
28	18.715304086965933	26.989866741525187	31.283328000456763	23.011501171052114
29	20.022433766267753	24.849975674945906	29.331138329267265	25.79645222951908
30	20.45172041770103	27.00057214430407	29.048396746984945	23.49931069100996
31	21.450058701291905	26.691423901833833	27.94264758884592	23.91586980802834
32	21.91205630566066	27.649557450544016	26.328867594388942	24.109518649406386
33	20.377734189606958	27.87294352186341	27.276057842480704	24.473264446048933
34	20.609446685309926	26.024715206548855	29.774698851072383	23.59113925706884
35	20.432212794859502	25.62242995990232	27.565460564269884	26.37989668096829
36	20.59136644950559	28.09240427883054	27.909341891311612	23.406887380352256
37	19.84079876578601	26.909219373924252	29.795633860951092	23.454347999338644
38	20.35549074161083	27.917787264614958	27.74543027987491	23.9812917138993
39	20.80035970153337	27.03661366699298	28.499328533347924	23.66369809812572
40	22.152927868185564	25.848313958536785	29.64575822204671	22.35299995123094
41	19.22940131819193	27.12297058274265	29.1358242030125	24.511803896052918
42	21.746717307186774	25.619694134747718	28.90720437922343	23.726384178842082
43	21.322783357142942	26.628024127598888	27.505153461948833	24.544039053309337
44	20.24879356058128	25.50026941930327	28.51907405402898	25.73186296608647
45	20.245819837587145	24.795853916452657	27.91957149841144	27.03875474754876
46	22.926214795580336	26.639086377137065	26.995457340754157	23.439241486528438
47	19.5848206904509	25.54951427208614	31.25858662514556	23.6070784123174
48	20.09285152676886	25.38191524413671	28.839165597117628	25.6860676319768
49	20.433521232976922	23.965947303249564	31.29605553487166	24.30447592890185
50	20.372143590377984	24.08489622301495	30.59259162337917	24.950368563227894
51	19.766574639852408	23.80881578023949	28.09157163639218	28.333037943515915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	684.5
2	1085.0
3	2489.5
4	3894.0
5	2700.0
6	1506.0
7	1497.5
8	1489.0
9	1527.5
10	1566.0
11	1564.5
12	1563.0
13	1683.0
14	1803.0
15	1751.0
16	1699.0
17	1848.0
18	1997.0
19	2064.5
20	2132.0
21	2187.5
22	2243.0
23	2528.5
24	2814.0
25	3638.0
26	6473.0
27	8484.0
28	9651.5
29	10819.0
30	12784.5
31	14750.0
32	17150.5
33	19551.0
34	22644.5
35	25738.0
36	27447.0
37	29156.0
38	31358.0
39	33560.0
40	37012.0
41	40464.0
42	45085.5
43	49707.0
44	55774.5
45	61842.0
46	81183.5
47	100525.0
48	96041.0
49	91557.0
50	86941.5
51	82326.0
52	70641.0
53	58956.0
54	51482.0
55	44008.0
56	39601.5
57	35195.0
58	32612.0
59	30029.0
60	27591.0
61	25153.0
62	21991.5
63	18830.0
64	16018.0
65	13206.0
66	10168.0
67	7130.0
68	5665.5
69	4201.0
70	3553.5
71	2906.0
72	2271.0
73	1636.0
74	1498.0
75	965.0
76	570.0
77	425.5
78	281.0
79	209.0
80	137.0
81	78.5
82	20.0
83	20.5
84	21.0
85	18.0
86	15.0
87	11.5
88	8.0
89	8.0
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.273854585444166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97159552981576	21.36159876407674
2	10.801295667981407	6.323911174377514
3	3.8011636077303574	3.338241321245424
4	1.9422195182877264	2.2742500700546526
5	1.1794193452192694	1.7263075203604334
6	0.833662117710354	1.4642702164347805
7	0.6304097397621024	1.2918166135730438
8	0.4956819628771681	1.1608417361515002
9	0.4218336269831511	1.1113826629999737
>10	6.442300397445452	45.07579225947623
>50	0.4119037957791012	7.252118094734455
>100	0.06035817964527936	2.9951623654027473
>500	0.003670429843294015	0.7514170889606542
>1k	0.0032626043051502355	1.637860032324629
>5k	0.0012234766144313383	2.235030079827202
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC	6894	0.8200338528625651	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG	6149	0.7314169076373533	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	5678	0.6753919664278568	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	3447	0.41001692643128257	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2178	0.25907074724900886	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	2004	0.23837363520983185	No Hit
CTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGCT	1559	0.18544136591423543	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT	1287	0.15308725973805068	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCATGAT	1143	0.13595861529183523	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT	1087	0.12929747578497366	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	1014	0.12061420464210054	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCC	996	0.11847312408632361	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTCATGATCGTA	928	0.11038459754227742	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC	880	0.10467504939353893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.757956790615406E-4	0.0	0.0	0.19305409677922009	0.0
2	4.757956790615406E-4	0.0	0.0	1.0886205136928049	0.0
3	4.757956790615406E-4	0.0	0.0	1.4848393654313028	0.0
4	4.757956790615406E-4	0.0	0.0	2.0780376283012787	0.0
5	4.757956790615406E-4	0.0	0.0	3.961117977107091	0.0
6	4.757956790615406E-4	0.0	0.0	4.8211186670108255	0.0
7	4.757956790615406E-4	0.0	0.0	5.718945113399952	0.0
8	4.757956790615406E-4	0.0	0.0	6.960771835750574	0.0
9	5.947445988269258E-4	0.0	0.0	7.383516296596753	0.0
10	5.947445988269258E-4	0.0	0.0	8.983022420681886	0.0
11	5.947445988269258E-4	0.0	0.0	10.265410724672504	0.0
12	5.947445988269258E-4	0.0	0.0	12.125414983043832	0.0
13	5.947445988269258E-4	0.0	0.0	12.613224503001677	0.0
14	5.947445988269258E-4	0.0	0.0	12.816151360121424	0.0
15	5.947445988269258E-4	0.0	0.0	13.23663579149206	0.0
16	5.947445988269258E-4	0.0	0.0	13.895018062393467	0.0
17	5.947445988269258E-4	0.0	0.0	14.720523565565239	0.0
18	5.947445988269258E-4	0.0	0.0	15.609666740811493	0.0
19	5.947445988269258E-4	0.0	0.0	16.303733687642517	0.0
20	5.947445988269258E-4	0.0	0.0	16.86112832566311	0.0
21	5.947445988269258E-4	0.0	0.0	17.53485500721425	0.0
22	5.947445988269258E-4	0.0	0.0	18.257469694788966	0.0
23	7.136935185923109E-4	0.0	0.0	18.979132791005558	0.0
24	7.136935185923109E-4	0.0	0.0	19.531888421155305	0.0
25	8.32642438357696E-4	0.0	0.0	20.027072774138603	0.0
26	8.32642438357696E-4	0.0	0.0	20.506080074033807	0.0
27	8.32642438357696E-4	0.0	0.0	20.99317590047306	0.0
28	9.515913581230812E-4	0.0	0.0	21.47004211981249	0.0
29	9.515913581230812E-4	0.0	0.0	21.958565333288927	0.0
30	9.515913581230812E-4	0.0	0.0	22.543437171775324	0.0
31	9.515913581230812E-4	0.0	0.0	23.071332477694103	0.0
32	9.515913581230812E-4	0.0	0.0	23.5854297089201	0.0
33	9.515913581230812E-4	0.0	0.0	24.098456399868205	0.0
34	9.515913581230812E-4	0.0	0.0	24.607557776464052	0.0
35	9.515913581230812E-4	0.0	0.0	25.136166775901426	0.0
36	9.515913581230812E-4	0.0	0.0	25.6303995375266	0.0
37	9.515913581230812E-4	0.0	0.0	26.142117790357286	0.0
38	9.515913581230812E-4	0.0	0.0	26.648126495039236	0.0
39	9.515913581230812E-4	0.0	0.0	27.166505887376783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTC	20	7.032203E-4	45.000004	32
AGTCCGT	20	7.032203E-4	45.000004	14
AATTTCG	20	7.032203E-4	45.000004	37
GTCGAGA	20	7.032203E-4	45.000004	2
CAACCGA	20	7.032203E-4	45.000004	24
GTTAACG	20	7.032203E-4	45.000004	1
TACGAGA	20	7.032203E-4	45.000004	1
CGTACCG	20	7.032203E-4	45.000004	2
ACGTAAG	35	1.2114106E-7	45.000004	1
ACGACAG	20	7.032203E-4	45.000004	1
CCGATCG	25	3.889908E-5	45.0	40
TGTACCG	25	3.889908E-5	45.0	1
TCGCGCG	25	3.889908E-5	45.0	1
CGTTTCA	70	0.0	41.785717	11
CGTTTTT	1415	0.0	40.865723	1
TCTCGAG	50	1.0804797E-9	40.5	1
ACGGGTA	90	0.0	40.0	5
ACGGCTG	865	0.0	39.53757	8
TACGGCT	860	0.0	39.505814	7
TCGTGTA	75	0.0	39.000004	17
>>END_MODULE