##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611077
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.135613024217896	33.0	31.0	34.0	30.0	34.0
2	32.14120151797564	33.0	31.0	34.0	30.0	34.0
3	32.15737951191094	33.0	31.0	34.0	30.0	34.0
4	35.82387489628967	37.0	35.0	37.0	35.0	37.0
5	35.8866967665286	37.0	35.0	37.0	35.0	37.0
6	35.819369735728884	37.0	35.0	37.0	35.0	37.0
7	36.07425741764131	37.0	35.0	37.0	35.0	37.0
8	35.842589395444435	37.0	35.0	37.0	35.0	37.0
9	37.669265902660385	39.0	38.0	39.0	35.0	39.0
10	37.35535947188325	39.0	37.0	39.0	34.0	39.0
11	37.345704387499445	39.0	37.0	39.0	34.0	39.0
12	37.22172164882658	39.0	37.0	39.0	34.0	39.0
13	37.12803623765908	39.0	37.0	39.0	33.0	39.0
14	38.278940297212955	40.0	37.0	41.0	33.0	41.0
15	38.388857705330096	40.0	38.0	41.0	34.0	41.0
16	38.20571220975425	40.0	37.0	41.0	34.0	41.0
17	38.240555609194914	40.0	37.0	41.0	34.0	41.0
18	38.176817324167004	40.0	37.0	41.0	34.0	41.0
19	38.05049281841732	40.0	37.0	41.0	34.0	41.0
20	38.161388826612686	40.0	37.0	41.0	34.0	41.0
21	38.10478875820887	40.0	37.0	41.0	34.0	41.0
22	38.19453031287383	40.0	37.0	41.0	34.0	41.0
23	38.231564925533114	40.0	37.0	41.0	34.0	41.0
24	38.149830545086786	40.0	37.0	41.0	34.0	41.0
25	37.91932113301597	40.0	36.0	41.0	34.0	41.0
26	37.97509479165473	40.0	36.0	41.0	34.0	41.0
27	37.996208333810635	40.0	36.0	41.0	34.0	41.0
28	37.88338294519349	40.0	36.0	41.0	34.0	41.0
29	37.859181412489754	40.0	36.0	41.0	34.0	41.0
30	37.73754535025864	40.0	36.0	41.0	33.0	41.0
31	37.72259633401355	40.0	36.0	41.0	33.0	41.0
32	37.67427345490012	40.0	36.0	41.0	33.0	41.0
33	37.618666714669345	40.0	36.0	41.0	33.0	41.0
34	37.53840023434035	40.0	36.0	41.0	33.0	41.0
35	37.490823578697935	40.0	36.0	41.0	33.0	41.0
36	37.39279501601271	40.0	35.0	41.0	33.0	41.0
37	37.39229917015368	40.0	35.0	41.0	33.0	41.0
38	37.24900298980325	40.0	35.0	41.0	33.0	41.0
39	37.19497870153843	40.0	35.0	41.0	32.0	41.0
40	37.0733442757623	40.0	35.0	41.0	32.0	41.0
41	36.932258946090265	39.0	35.0	41.0	32.0	41.0
42	36.95790383208663	39.0	35.0	41.0	32.0	41.0
43	36.91261821341664	39.0	35.0	41.0	32.0	41.0
44	36.815370239757016	39.0	35.0	41.0	32.0	41.0
45	36.799277341480696	39.0	35.0	41.0	31.0	41.0
46	36.65418269710691	39.0	35.0	41.0	31.0	41.0
47	36.581738471583776	39.0	35.0	41.0	31.0	41.0
48	36.51085051474691	39.0	35.0	41.0	31.0	41.0
49	36.457232067317214	39.0	35.0	41.0	31.0	41.0
50	36.33660733426393	39.0	35.0	41.0	31.0	41.0
51	35.196446601655765	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	13.0
10	13.0
11	13.0
12	8.0
13	16.0
14	19.0
15	34.0
16	46.0
17	74.0
18	129.0
19	280.0
20	445.0
21	816.0
22	1346.0
23	1773.0
24	2428.0
25	3308.0
26	4443.0
27	4897.0
28	5688.0
29	6395.0
30	7934.0
31	10221.0
32	13561.0
33	19227.0
34	36654.0
35	51987.0
36	41203.0
37	62510.0
38	114641.0
39	220805.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.054186297307865	20.145579035047955	23.49262040626631	12.307614261377863
2	30.97874735917078	27.2788862941986	24.161439556717074	17.580926789913544
3	31.475247800195394	25.846497249937407	26.001796827568374	16.676458122298826
4	28.799152970902192	24.768727999908357	27.37363703755828	19.05848199163117
5	27.251884787023567	28.560885125769747	24.387597634995263	19.799632452211423
6	25.622466563133617	35.288351549804695	24.15702112827025	14.932160758791447
7	77.21498272721769	6.22965681902608	11.47400409441036	5.081356359345876
8	77.10124910608647	8.487310109855223	9.107362901892888	5.304077882165423
9	70.72856612178171	8.745051769253303	11.103019750375157	9.423362358589834
10	41.081074889089265	25.678596968958082	18.44628418349897	14.794043958453681
11	32.094973301237	25.48631350877222	23.822857021291917	18.59585616869887
12	29.8150642226757	22.1116160483867	28.211665632972604	19.861654095964994
13	24.790656496644452	25.313340217353954	29.623926280976047	20.272077005025555
14	19.731392279532695	29.338364886912778	27.683090674334004	23.247152159220523
15	18.85932542052802	25.452275245181866	35.122251369303704	20.56614796498641
16	20.915531103281584	25.28764787416316	31.39097036870967	22.405850653845587
17	20.90227581794111	25.48811360925055	28.924178131397515	24.68543244141082
18	21.93749723848222	25.981832076808654	29.40153204915256	22.679138635556566
19	24.262899765496	27.59455845989949	26.20504453612229	21.93749723848222
20	27.3957291797924	25.383216845012985	27.6793268278793	19.541727147315314
21	24.735835254804222	28.31558052422199	26.717909526949956	20.230674694023833
22	22.05499470606814	25.613956997236027	27.65068886572396	24.680359430971873
23	22.236641208882023	27.58490337551569	27.76900456080003	22.40945085480226
24	22.800072658601124	24.152766345321456	29.29548976642878	23.751671229648636
25	21.74144993184165	28.100877630805936	26.72903742081603	23.42863501653638
26	20.674317639184586	28.1574989731245	27.52910026068728	23.639083127003634
27	22.38523132109374	27.655434585166844	27.4016204177215	22.557713676017915
28	19.412610849369226	27.804842924868716	30.195212714600615	22.587333511161443
29	21.892167435527764	26.76160287492411	28.388566416343604	22.957663273204524
30	21.631480157165136	27.449732194142474	27.83659015148664	23.082197497205755
31	23.095943719040317	27.39834750776089	26.19669861572273	23.309010157476063
32	25.748146305621063	27.934450159308895	25.55258993547458	20.76481359959547
33	23.253207042647652	27.377891820507074	25.681215296926574	23.6876858399187
34	21.196837714396057	27.726293085814063	28.17615455989998	22.900714639889898
35	22.704340042253268	25.734400083786497	26.4763687718569	25.084891102103335
36	21.25967758563978	29.250650899968416	26.438893952807913	23.050777561583892
37	23.070578666845588	27.220464851401704	27.948032735645427	21.760923746107284
38	22.157273142337218	28.2196842623761	26.06201837084361	23.561024224443074
39	23.60668131839359	25.867116582689253	27.82709871260087	22.699103386316292
40	24.037887205704028	25.813604504833272	28.487081006157982	21.661427283304725
41	20.0750478253968	25.749946406099394	28.38709360686133	25.787912161642478
42	22.13387183611885	26.059072751879057	27.90204835069885	23.90500706130324
43	23.5048938186186	25.045125246081916	26.625940757056803	24.824040178242676
44	22.059576780012993	25.702816502666604	27.790933057536122	24.44667365978428
45	21.665845711751547	25.29795754053908	27.14993364175055	25.88626310595882
46	22.585697056181136	27.483115875740697	26.283267084180878	23.647919983897285
47	20.919622240732348	25.415291362626967	30.489610965557535	23.175475431083154
48	22.06415885395785	24.561389153903683	28.53993850202184	24.83451349011663
49	21.209274772246378	24.51949590640787	29.703294347520853	24.567934973824904
50	20.3822104252001	25.140530571433718	29.69003906218038	24.78721994118581
51	20.861855379927572	24.389561380971628	27.191335952752272	27.55724728634853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	209.0
1	795.0
2	1381.0
3	2044.5
4	2708.0
5	1919.5
6	1131.0
7	1122.5
8	1114.0
9	1170.5
10	1227.0
11	1244.0
12	1261.0
13	1200.5
14	1140.0
15	1261.5
16	1383.0
17	1352.0
18	1321.0
19	1611.5
20	1902.0
21	2051.0
22	2200.0
23	2334.0
24	2468.0
25	3049.5
26	4554.5
27	5478.0
28	6850.5
29	8223.0
30	9143.5
31	10064.0
32	11501.5
33	12939.0
34	15583.5
35	18228.0
36	18376.0
37	18524.0
38	20329.0
39	22134.0
40	25104.0
41	28074.0
42	31090.0
43	34106.0
44	37177.0
45	40248.0
46	50632.0
47	61016.0
48	58409.0
49	55802.0
50	53906.0
51	52010.0
52	46163.5
53	40317.0
54	36878.5
55	33440.0
56	30560.5
57	27681.0
58	28128.0
59	28575.0
60	27610.5
61	26646.0
62	24192.5
63	21739.0
64	18630.0
65	15521.0
66	12870.0
67	10219.0
68	8284.0
69	6349.0
70	5409.0
71	4469.0
72	3677.0
73	2885.0
74	2191.0
75	1212.5
76	928.0
77	749.0
78	570.0
79	376.0
80	182.0
81	134.5
82	87.0
83	53.0
84	19.0
85	15.0
86	11.0
87	10.5
88	10.0
89	8.5
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	611077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.108610106265463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29892939618367	19.870320982078233
2	10.252295918368908	5.5585098549023915
3	3.5710258678043116	2.904166437890861
4	1.7061501819764728	1.8500544026373629
5	1.0912342092008076	1.479092135592181
6	0.7295151418711506	1.1865684928561173
7	0.5921796745937289	1.1237217537991586
8	0.5090941958957229	1.1040668851119906
9	0.4347015150775843	1.0605738496357
>10	7.2112595601248985	48.38578792283151
>50	0.5345259507151017	8.734732640965348
>100	0.058785733808803704	2.848107379836652
>500	0.006060384928742649	1.069518864947889
>1k	0.004242269450119855	2.8247783969145934
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	4161	0.6809289173050205	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	3935	0.6439450347501214	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	3276	0.5361026515480046	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1877	0.30716259980329813	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1356	0.22190329532939382	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1351	0.2210850678392412	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1238	0.20259312656179174	No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	948	0.15513593213293905	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	789	0.12911629794608537	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT	695	0.11373362113121586	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	655	0.10718780120999481	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	634	0.10375124575135376	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.182274901526322E-4	0.0	0.0	0.16020894257188537	0.0
2	8.182274901526322E-4	0.0	0.0	0.882540170878629	0.0
3	8.182274901526322E-4	0.0	0.0	1.2096675214416515	0.0
4	8.182274901526322E-4	0.0	0.0	1.6850576932203307	0.0
5	8.182274901526322E-4	0.0	0.0	3.1899416931090516	0.0
6	8.182274901526322E-4	0.0	0.0	3.857124388579508	0.0
7	8.182274901526322E-4	0.0	0.0	4.5979475581637015	0.0
8	8.182274901526322E-4	0.0	0.0	5.584893556785806	0.0
9	8.182274901526322E-4	0.0	0.0	5.958823519785559	0.0
10	8.182274901526322E-4	0.0	0.0	7.352428581013522	0.0
11	8.182274901526322E-4	0.0	0.0	8.451635391284569	0.0
12	8.182274901526322E-4	0.0	0.0	9.947846179777672	0.0
13	8.182274901526322E-4	0.0	0.0	10.371524374178705	0.0
14	8.182274901526322E-4	0.0	0.0	10.561516797392146	0.0
15	8.182274901526322E-4	0.0	0.0	10.92988281345886	0.0
16	8.182274901526322E-4	0.0	0.0	11.53275282820332	0.0
17	8.182274901526322E-4	0.0	0.0	12.190771375784067	0.0
18	8.182274901526322E-4	0.0	0.0	12.945504412700855	0.0
19	8.182274901526322E-4	0.0	0.0	13.479970609268554	0.0
20	9.818729881831585E-4	0.0	0.0	13.939978104232363	0.0
21	9.818729881831585E-4	0.0	0.0	14.518628585268305	0.0
22	9.818729881831585E-4	0.0	0.0	15.119862145032458	0.0
23	9.818729881831585E-4	0.0	0.0	15.693275970131424	0.0
24	9.818729881831585E-4	0.0	0.0	16.166375104937675	0.0
25	9.818729881831585E-4	0.0	0.0	16.58367112491552	0.0
26	9.818729881831585E-4	0.0	0.0	16.959728479389668	0.0
27	9.818729881831585E-4	0.0	0.0	17.351495801674748	0.0
28	9.818729881831585E-4	0.0	0.0	17.747517906908623	0.0
29	9.818729881831585E-4	0.0	0.0	18.161213725929795	0.0
30	9.818729881831585E-4	0.0	0.0	18.64560440010015	0.0
31	9.818729881831585E-4	0.0	0.0	19.078119451394834	0.0
32	9.818729881831585E-4	0.0	0.0	19.51636209512058	0.0
33	9.818729881831585E-4	0.0	0.0	19.92891239565554	0.0
34	9.818729881831585E-4	0.0	0.0	20.33622604025352	0.0
35	9.818729881831585E-4	0.0	0.0	20.773977747485176	0.0
36	9.818729881831585E-4	0.0	0.0	21.201419788340914	0.0
37	0.001145518486213685	0.0	0.0	21.633280257643474	0.0
38	0.001145518486213685	0.0	0.0	22.03617547379463	0.0
39	0.001145518486213685	0.0	0.0	22.477200090986898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	25	3.8886537E-5	45.0	31
ACCGGTA	20	7.0306903E-4	45.0	41
CGTATGA	20	7.0306903E-4	45.0	2
CGAAACA	20	7.0306903E-4	45.0	32
AATTGCG	45	3.8380676E-10	45.0	1
CTACGCG	20	7.0306903E-4	45.0	1
CGTGTAA	20	7.0306903E-4	45.0	20
CCGAATA	20	7.0306903E-4	45.0	13
TCATACG	25	3.8886537E-5	45.0	31
TAAGCCG	20	7.0306903E-4	45.0	38
GCGTATT	20	7.0306903E-4	45.0	10
GACTCGT	20	7.0306903E-4	45.0	38
ATCGAAG	25	3.8886537E-5	45.0	12
GCTACGA	195	0.0	42.692307	10
TACGAAT	200	0.0	41.625	12
TCGTGTA	55	6.002665E-11	40.909092	17
CTAGCGG	55	6.002665E-11	40.909092	2
TGACCGG	50	1.0804797E-9	40.5	2
CGGATCT	50	1.0804797E-9	40.5	29
CGAATAT	195	0.0	40.384617	14
>>END_MODULE