SRR2935575.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	10089	0.9287079278463254	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	10041	0.9242894542080438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	9690	0.8919793657281093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6993	0.6437163781771587	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	3026	0.27854794228000607	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	2438	0.2244216402110558	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1953	0.17977664615758487	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1925	0.17719920320192056	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	1667	0.15344990739615666	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	1581	0.14553347546090203	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1461	0.13448729136519788	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1164	0.10714798572833013	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1140	0.10493874890918932	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	25	3.890664E-5	45.0	23
AAACTCG	25	3.890664E-5	45.0	39
GTATACG	20	7.033116E-4	45.0	1
ACCGCTC	50	1.0822987E-9	40.5	18
CGTTTTT	3145	0.0	40.206676	1
TACGGCT	1310	0.0	39.50382	7
CTTGACG	40	3.4579898E-7	39.375	1
GATCGAT	35	6.247974E-6	38.571426	9
TCGATAG	35	6.247974E-6	38.571426	1
TAGCGAT	30	1.1398807E-4	37.499996	12
TGCGATA	30	1.1398807E-4	37.499996	1
TATTACG	30	1.1398807E-4	37.499996	1
CGGTACC	30	1.1398807E-4	37.499996	14
ACGGCTG	1405	0.0	36.99288	8
GCCGACC	55	2.748493E-9	36.818184	14
GCGTACG	55	2.748493E-9	36.818184	1
CATACGA	230	0.0	36.195652	18
TAATACG	25	0.002107058	36.0	22
GTCGACT	25	0.002107058	36.0	9
CGAAACG	25	0.002107058	36.0	37