##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.098603762330306	33.0	31.0	34.0	30.0	34.0
2	32.12365190528266	33.0	31.0	34.0	30.0	34.0
3	32.116605360344934	33.0	31.0	34.0	30.0	34.0
4	35.78161049682054	37.0	35.0	37.0	35.0	37.0
5	35.843433227658174	37.0	35.0	37.0	35.0	37.0
6	35.77471215485277	37.0	35.0	37.0	35.0	37.0
7	36.03383170033912	37.0	35.0	37.0	35.0	37.0
8	35.81640413569133	37.0	35.0	37.0	35.0	37.0
9	37.63994778836984	39.0	37.0	39.0	35.0	39.0
10	37.308925869058534	39.0	37.0	39.0	34.0	39.0
11	37.28711978113827	39.0	37.0	39.0	34.0	39.0
12	37.16404411845928	39.0	37.0	39.0	34.0	39.0
13	37.07084101963643	39.0	37.0	39.0	33.0	39.0
14	38.10913629886556	40.0	37.0	41.0	33.0	41.0
15	38.207704161097546	40.0	37.0	41.0	33.0	41.0
16	38.119532599130295	40.0	37.0	41.0	33.0	41.0
17	38.13975908272487	40.0	37.0	41.0	33.0	41.0
18	38.05291030130308	40.0	37.0	41.0	33.0	41.0
19	37.904328079031764	40.0	37.0	41.0	33.0	41.0
20	37.941438655016626	40.0	36.0	41.0	33.0	41.0
21	37.944619956036185	40.0	36.0	41.0	33.0	41.0
22	38.053527046581756	40.0	36.0	41.0	34.0	41.0
23	38.05651043680294	40.0	36.0	41.0	34.0	41.0
24	37.97672108753365	40.0	36.0	41.0	34.0	41.0
25	37.776972020015684	40.0	36.0	41.0	33.0	41.0
26	37.81194792092405	40.0	36.0	41.0	33.0	41.0
27	37.791111135658184	40.0	36.0	41.0	33.0	41.0
28	37.65351526398539	40.0	35.0	41.0	33.0	41.0
29	37.6262155405082	40.0	35.0	41.0	33.0	41.0
30	37.45684624079945	40.0	35.0	41.0	33.0	41.0
31	37.41978813418905	40.0	35.0	41.0	33.0	41.0
32	37.296949043952765	40.0	35.0	41.0	33.0	41.0
33	37.19013612580867	40.0	35.0	41.0	32.0	41.0
34	37.083976773556905	40.0	35.0	41.0	32.0	41.0
35	36.93972557596645	40.0	35.0	41.0	31.0	41.0
36	36.836556978058596	40.0	35.0	41.0	31.0	41.0
37	36.78660981563919	40.0	35.0	41.0	31.0	41.0
38	36.66660038956209	39.0	35.0	41.0	31.0	41.0
39	36.604985695191594	39.0	35.0	41.0	31.0	41.0
40	36.48619134936503	39.0	35.0	41.0	30.0	41.0
41	36.30050591523158	39.0	35.0	41.0	30.0	41.0
42	36.345926903717775	39.0	35.0	41.0	30.0	41.0
43	36.28377831044932	39.0	35.0	41.0	30.0	41.0
44	36.20066405976722	39.0	35.0	41.0	30.0	41.0
45	36.194292252574684	39.0	35.0	41.0	30.0	41.0
46	36.092747443728896	39.0	35.0	41.0	30.0	41.0
47	35.954502608740476	38.0	35.0	41.0	29.0	41.0
48	35.87379826722192	38.0	35.0	40.0	29.0	41.0
49	35.83956061961729	38.0	35.0	40.0	29.0	41.0
50	35.6916899557048	38.0	35.0	40.0	29.0	41.0
51	34.55603913294819	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	24.0
10	28.0
11	34.0
12	26.0
13	33.0
14	37.0
15	56.0
16	107.0
17	186.0
18	342.0
19	710.0
20	1462.0
21	2297.0
22	3270.0
23	4416.0
24	5794.0
25	7943.0
26	10341.0
27	11720.0
28	12359.0
29	13799.0
30	16477.0
31	20339.0
32	26970.0
33	38560.0
34	70076.0
35	82662.0
36	77543.0
37	114390.0
38	200699.0
39	363427.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77934510856558	18.819475895385274	22.016977984955098	13.384201011094051
2	33.05193179349527	27.261798245129555	23.220643845250326	16.465626116124852
3	31.277362318520403	26.416765161808186	26.414924131125566	15.890948388545844
4	27.742583407895076	23.739538343146027	30.148166149337047	18.36971209962185
5	27.8896817594362	27.846509589928825	24.969807096805074	19.294001553829894
6	24.5854919418087	35.23465777080641	25.697290371041326	14.482559916343565
7	75.96304315007714	6.031032413186198	13.515742653367061	4.490181783369602
8	76.37847172360975	8.636735189828673	10.43312087839256	4.5516722081690215
9	70.61310003792524	7.212053596085233	12.328461966147128	9.846384399842407
10	42.209586614970526	22.943752830584675	19.544289675131726	15.302370879313074
11	32.26995401105355	24.642932099106364	25.18465537746652	17.902458512373567
12	28.230824744925197	21.157676913843446	29.997385736430683	20.61411260480067
13	26.035671810506393	21.90670024706632	33.15180770802726	18.905820234400025
14	21.58967476351961	26.363743478148805	31.350175082017916	20.696406676313668
15	20.02415432255594	24.629768729725647	35.3208180067529	20.02525894096551
16	23.80968161215375	24.124405807347184	30.24923873381274	21.816673846686328
17	24.263127469282402	24.36373979608744	29.53887704492483	21.834255689705326
18	24.45395029953569	24.028119902646296	29.76145765445327	21.756472143364743
19	24.61605305114015	25.760529774989234	27.378887796544017	22.244529377326604
20	24.80024817093602	26.442631642898963	29.77756667292617	18.97955351323885
21	24.294240887818635	27.175914163785453	29.80831188532588	18.721533063070027
22	23.24043492508846	22.948999768030134	31.23998939566327	22.570575911218135
23	22.81165887910688	25.745525374925897	30.405910444903473	21.036905301063747
24	22.903250155567093	24.88530378847294	29.150143416290174	23.061302639669794
25	22.952129520190585	27.63248977307456	27.69232327025962	21.723057436475237
26	21.771844749564593	25.241911431695918	28.961990080526682	24.0242537382128
27	23.773137153103793	25.990382455714006	28.535331219830113	21.701149171352093
28	21.569975735215603	26.344688810583715	29.785851310997952	22.29948414320273
29	24.272608777297883	25.39987186426449	28.189677709168702	22.13784164926893
30	23.765773030373325	26.888805428831276	28.69375191006933	20.651669630726065
31	25.67906416728341	25.28950207484158	26.657387872026273	22.374045885848734
32	26.163347288345907	25.891611159591587	26.7660086823007	21.17903286976181
33	24.60519097011271	25.040594726551713	27.58149322316606	22.77272108016952
34	24.074790030450647	25.96663315990824	28.63631175277167	21.322265056869437
35	22.384723863807913	26.745112984052994	28.59507266548104	22.27509048665805
36	26.873248719563158	26.466657093307116	26.213607425981362	20.44648676114836
37	22.831173804342622	28.23432270322217	27.611409971758587	21.323093520676615
38	23.793388490612585	25.600636260203913	27.800115616726867	22.805859632456635
39	23.264000117825965	25.710729895024432	27.409540957409597	23.61572902974001
40	23.75785659843807	24.029316572589998	31.05330888444587	21.159517944526062
41	22.35848917658062	26.063011116143265	27.78373044365157	23.79476926362455
42	22.20283003236532	24.874441707445495	30.691270200709162	22.23145805948002
43	24.33851767573558	24.733326705622876	27.861329886923897	23.066825731717643
44	23.537117019592248	24.89193149893036	28.135919613236275	23.435031868241115
45	22.015689263477263	24.507156086263336	27.47747498959818	25.999679660661222
46	23.971784363758207	25.877895481006085	27.47471344357425	22.675606711661455
47	21.02318962247825	25.382474124313752	31.90441736901987	21.689918884188124
48	22.046526527411107	26.581353304834177	28.282925913243268	23.089194254511447
49	22.348547610894485	24.353798230401306	30.52465692393229	22.772997234771914
50	21.647391075419662	24.275922632526594	29.692511055389247	24.384175236664493
51	21.835176205046633	24.538177453265437	27.726658492490436	25.899987849197494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	502.0
1	1202.5
2	1903.0
3	6367.0
4	10831.0
5	7027.0
6	3223.0
7	3205.0
8	3187.0
9	3125.5
10	3064.0
11	3110.5
12	3157.0
13	3198.5
14	3240.0
15	3161.5
16	3083.0
17	3153.5
18	3224.0
19	3297.0
20	3370.0
21	3764.0
22	4158.0
23	4873.0
24	5588.0
25	6348.5
26	8454.5
27	9800.0
28	10791.0
29	11782.0
30	13796.0
31	15810.0
32	19371.5
33	22933.0
34	25708.0
35	28483.0
36	30454.0
37	32425.0
38	34920.5
39	37416.0
40	42220.0
41	47024.0
42	49916.0
43	52808.0
44	57917.0
45	63026.0
46	88567.5
47	114109.0
48	102706.0
49	91303.0
50	90468.5
51	89634.0
52	80724.0
53	71814.0
54	67565.0
55	63316.0
56	60551.5
57	57787.0
58	55745.5
59	53704.0
60	49698.0
61	45692.0
62	41161.0
63	36630.0
64	31577.0
65	26524.0
66	23058.0
67	19592.0
68	17120.0
69	14648.0
70	12142.0
71	9636.0
72	8117.5
73	6599.0
74	5262.5
75	3026.5
76	2127.0
77	1724.0
78	1321.0
79	884.0
80	447.0
81	312.5
82	178.0
83	146.0
84	114.0
85	85.5
86	57.0
87	36.0
88	15.0
89	13.5
90	12.0
91	7.0
92	2.0
93	4.5
94	7.0
95	6.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1086348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.8364216010734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9093721235359	19.834630705778313
2	9.202943464456546	4.939481415659979
3	3.3781129610208676	2.7196939091401933
4	1.8212253917245231	1.9550068977159745
5	1.10189331479084	1.4785436777565637
6	0.8074999528925455	1.3002245507202759
7	0.6592845649372093	1.2384986977814643
8	0.5547376945552401	1.1909739719273527
9	0.4867098592425806	1.1755395882029687
>10	7.575419016744095	48.95450823391205
>50	0.4458612091179044	7.258651217087321
>100	0.04969348100227533	2.437700905465481
>500	0.002070561708427655	0.4012825022179364
>1k	0.0037960297987840344	1.7059831464012476
>5k	6.901872361425517E-4	1.5450256137784057
>10k+	6.901872361425517E-4	1.864254966454433
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	10089	0.9287079278463254	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	10041	0.9242894542080438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	9690	0.8919793657281093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6993	0.6437163781771587	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	3026	0.27854794228000607	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	2438	0.2244216402110558	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1953	0.17977664615758487	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1925	0.17719920320192056	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	1667	0.15344990739615666	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	1581	0.14553347546090203	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1461	0.13448729136519788	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1164	0.10714798572833013	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1140	0.10493874890918932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001012566875439546	0.0	0.0	0.09775872924698163	0.0
2	0.001012566875439546	0.0	0.0	0.5796485104220747	0.0
3	0.001012566875439546	0.0	0.0	0.8149322316605728	0.0
4	0.001012566875439546	0.0	0.0	1.2955332913578337	0.0
5	0.001012566875439546	0.0	0.0	2.8809368636937704	0.0
6	0.001012566875439546	0.0	0.0	3.5812649353614128	0.0
7	0.001012566875439546	0.0	0.0	4.261065514917871	0.0
8	0.001012566875439546	0.0	0.0	5.066056180892311	0.0
9	0.001012566875439546	0.0	0.0	5.377742675459429	0.0
10	0.001012566875439546	0.0	0.0	6.863822642468159	0.0
11	0.001012566875439546	0.0	0.0	7.686579254529856	0.0
12	0.001012566875439546	0.0	0.0	9.224208080651872	0.0
13	0.001012566875439546	0.0	0.0	9.540313048857271	0.0
14	0.001012566875439546	0.0	0.0	9.731412033712953	0.0
15	0.001012566875439546	0.0	0.0	10.061784989708638	0.0
16	0.001012566875439546	0.0	0.0	10.456962225732454	0.0
17	0.001012566875439546	0.0	0.0	10.889972642284055	0.0
18	0.001104618409570414	0.0	0.0	11.38677477198835	0.0
19	0.001104618409570414	0.0	0.0	11.860195812023402	0.0
20	0.001104618409570414	0.0	0.0	12.214778321495507	0.0
21	0.0011966699437012816	0.0	0.0	12.613453515816294	0.0
22	0.0011966699437012816	0.0	0.0	13.067727836752127	0.0
23	0.0011966699437012816	0.0	0.0	13.461156093627457	0.0
24	0.0011966699437012816	0.0	0.0	13.798617017751218	0.0
25	0.0011966699437012816	0.0	0.0	14.115550449763795	0.0
26	0.0011966699437012816	0.0	0.0	14.428341562740485	0.0
27	0.0011966699437012816	0.0	0.0	14.79065640107958	0.0
28	0.0011966699437012816	0.0	0.0	15.106301111614327	0.0
29	0.0011966699437012816	0.0	0.0	15.45563668364097	0.0
30	0.0011966699437012816	0.0	0.0	15.934304661121482	0.0
31	0.0011966699437012816	0.0	0.0	16.308310044295197	0.0
32	0.0011966699437012816	0.0	0.0	16.654699967229654	0.0
33	0.0012887214778321495	0.0	0.0	17.002654766244333	0.0
34	0.0012887214778321495	0.0	0.0	17.377396561691096	0.0
35	0.0012887214778321495	0.0	0.0	17.75959453140246	0.0
36	0.0012887214778321495	0.0	0.0	18.13083836855225	0.0
37	0.0012887214778321495	0.0	0.0	18.490759867003945	0.0
38	0.0013807730119630175	0.0	0.0	18.88676556683494	0.0
39	0.0013807730119630175	0.0	0.0	19.489058754653204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	25	3.890664E-5	45.0	23
AAACTCG	25	3.890664E-5	45.0	39
GTATACG	20	7.033116E-4	45.0	1
ACCGCTC	50	1.0822987E-9	40.5	18
CGTTTTT	3145	0.0	40.206676	1
TACGGCT	1310	0.0	39.50382	7
CTTGACG	40	3.4579898E-7	39.375	1
GATCGAT	35	6.247974E-6	38.571426	9
TCGATAG	35	6.247974E-6	38.571426	1
TAGCGAT	30	1.1398807E-4	37.499996	12
TGCGATA	30	1.1398807E-4	37.499996	1
TATTACG	30	1.1398807E-4	37.499996	1
CGGTACC	30	1.1398807E-4	37.499996	14
ACGGCTG	1405	0.0	36.99288	8
GCCGACC	55	2.748493E-9	36.818184	14
GCGTACG	55	2.748493E-9	36.818184	1
CATACGA	230	0.0	36.195652	18
TAATACG	25	0.002107058	36.0	22
GTCGACT	25	0.002107058	36.0	9
CGAAACG	25	0.002107058	36.0	37
>>END_MODULE