FastQCFastQC Report
Sat 14 Jan 2017
SRR2935573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497332
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC39530.7948412730329035No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC37560.7552299067825919Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG37360.7512084482800222No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC17900.35992053597998924RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14310.2877353558588629No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC14120.28391497028142165Illumina PCR Primer Index 5 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT14060.2827085327306507Illumina PCR Primer Index 5 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT9220.18538923696846374Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT8550.1719173509848552No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7530.15140791262174966No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT7440.14959825629559328No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT7190.14457143316738116RNA PCR Primer, Index 33 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA5560.11179654637143799No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC5420.10898152541963918RNA PCR Primer, Index 33 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC5090.10234611889039917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG351.2100645E-745.0000041
GTCGATG253.887603E-545.01
GTTTACG207.029423E-445.01
TTCACGG502.1827873E-1145.02
ACTTAGC253.887603E-545.039
GACCAAT207.029423E-445.024
CGGATAG207.029423E-445.018
ATAGTAG207.029423E-445.01
ATCGGAC207.029423E-445.031
TCGACAA253.887603E-545.019
TATCGTG253.887603E-545.01
TAGTCCG207.029423E-445.02
GTATACG302.1630094E-644.9999961
CGATGAA2600.042.4038519
TACGAAT700.041.78571712
CGGGTAT556.002665E-1140.9090926
TATTGCG501.0786607E-940.51
TACACGG451.9244908E-840.0000042
CCGATGA2800.039.37500418
CGTATGG403.4529512E-739.3752
Produced by FastQC (version 0.11.5)