##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497332
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09158871739603	33.0	31.0	34.0	30.0	34.0
2	32.11020404880442	33.0	31.0	34.0	30.0	34.0
3	32.092368880345525	33.0	31.0	34.0	30.0	34.0
4	35.76870782495396	37.0	35.0	37.0	35.0	37.0
5	35.837525033579176	37.0	35.0	37.0	35.0	37.0
6	35.77206373207435	37.0	35.0	37.0	35.0	37.0
7	36.021462524028216	37.0	35.0	37.0	35.0	37.0
8	35.841512309684475	37.0	35.0	37.0	35.0	37.0
9	37.6477322995504	39.0	37.0	39.0	35.0	39.0
10	37.354815294410976	39.0	37.0	39.0	34.0	39.0
11	37.313794406955516	39.0	37.0	39.0	34.0	39.0
12	37.07107123611591	39.0	35.0	39.0	34.0	39.0
13	36.888062702580974	39.0	35.0	39.0	33.0	39.0
14	37.790379464824305	40.0	36.0	41.0	33.0	41.0
15	37.9022644832828	40.0	36.0	41.0	33.0	41.0
16	37.838932544055076	40.0	36.0	41.0	33.0	41.0
17	37.91303797061118	40.0	36.0	41.0	33.0	41.0
18	37.838618870291874	39.0	36.0	41.0	33.0	41.0
19	37.73216885299961	39.0	36.0	41.0	33.0	41.0
20	37.7939706272671	40.0	36.0	41.0	33.0	41.0
21	37.68773776873397	39.0	35.0	41.0	33.0	41.0
22	37.85452172794029	40.0	35.0	41.0	34.0	41.0
23	37.89236164172022	40.0	35.0	41.0	34.0	41.0
24	37.859755656181385	40.0	35.0	41.0	34.0	41.0
25	37.620575390282546	39.0	35.0	41.0	33.0	41.0
26	37.69527398196778	40.0	35.0	41.0	33.0	41.0
27	37.6717544819155	40.0	35.0	41.0	33.0	41.0
28	37.51339949973056	40.0	35.0	41.0	33.0	41.0
29	37.37280126756372	39.0	35.0	41.0	33.0	41.0
30	37.25978822999525	39.0	35.0	41.0	33.0	41.0
31	37.21285780927027	39.0	35.0	41.0	33.0	41.0
32	37.069098710720404	39.0	35.0	41.0	32.0	41.0
33	36.98758575760257	39.0	35.0	41.0	32.0	41.0
34	36.75354089421151	39.0	35.0	41.0	31.0	41.0
35	36.73319432491776	39.0	35.0	41.0	31.0	41.0
36	36.68683294057089	39.0	35.0	41.0	31.0	41.0
37	36.58808602704028	39.0	35.0	41.0	31.0	41.0
38	36.43855010335148	39.0	35.0	41.0	31.0	41.0
39	36.28183185477709	39.0	35.0	41.0	30.0	41.0
40	36.23091214721755	38.0	35.0	41.0	30.0	41.0
41	36.04497599189274	38.0	35.0	40.0	30.0	41.0
42	36.15338244874651	38.0	35.0	41.0	30.0	41.0
43	36.0815310496811	38.0	35.0	40.0	30.0	41.0
44	36.00386462162097	38.0	35.0	40.0	30.0	41.0
45	35.966064922426064	38.0	35.0	40.0	30.0	41.0
46	35.83327234121271	38.0	35.0	40.0	30.0	41.0
47	35.744026123394434	37.0	35.0	40.0	30.0	41.0
48	35.65715055536342	37.0	35.0	40.0	30.0	41.0
49	35.604141700111796	37.0	35.0	40.0	29.0	41.0
50	35.44030748071711	37.0	35.0	40.0	29.0	41.0
51	34.24213000571047	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	4.0
10	12.0
11	8.0
12	10.0
13	12.0
14	19.0
15	29.0
16	36.0
17	86.0
18	153.0
19	293.0
20	543.0
21	947.0
22	1405.0
23	1889.0
24	2433.0
25	3223.0
26	4137.0
27	4873.0
28	5454.0
29	6365.0
30	7667.0
31	10120.0
32	13661.0
33	19698.0
34	38207.0
35	46860.0
36	37741.0
37	56623.0
38	94958.0
39	139771.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22230622602205	19.385842857487553	23.14791728664152	12.243933629848874
2	32.130246997981224	26.667296695165405	23.823924460923486	17.37853184592988
3	31.407993050919707	26.892297298384175	25.62413035959882	16.075579291097295
4	27.260461824294435	24.600065951919444	29.19438121818021	18.945091005605914
5	27.4140815390926	28.141764455132588	24.93485237225837	19.509301633516444
6	24.89644744355883	34.10056059131526	25.632977568304472	15.370014396821437
7	77.1140807348009	5.721530084531057	12.374832104107519	4.789557076560527
8	78.08224686929456	7.655248405491704	9.404582854109528	4.857921871104212
9	72.3014002718506	6.836077308518253	11.246611921211585	9.615910498419566
10	44.67498572382232	23.055423741082414	18.001656840903056	14.267933694192209
11	34.66436907337553	23.212461695607765	22.92311775634787	19.200051474668832
12	29.894919289327852	20.848849460722416	28.038010825766293	21.218220424183443
13	27.62078450612468	23.666283287622754	30.360000965150043	18.352931241102524
14	21.47338196617149	28.18901659253778	30.259464502585796	20.07813693870493
15	18.769956487819	22.76427014549637	37.89299703216363	20.572776334521002
16	22.509912895208835	25.303620116944014	30.174812801106704	22.011654186740447
17	24.285386824093365	22.923318829273	27.54095855484867	25.250335791784966
18	26.204828967369885	21.945501194373175	29.846862860222146	22.002806978034794
19	26.855098807235407	24.129354234193656	26.245646771170968	22.769900187399966
20	27.997595167815465	25.1383381724884	27.381708798146914	19.482357861549225
21	24.61595071300459	26.87098356832056	29.40188847691281	19.111177241762043
22	24.53250544907627	22.516146155887817	29.636942726388003	23.314405668647904
23	22.719833029042974	26.1477242566334	28.41622899793297	22.71621371639066
24	22.130086139641126	24.34309475360524	28.746189668068816	24.780629438684823
25	22.797045032292313	27.774404220922843	25.934989101847457	23.493561644937387
26	21.603074002879364	25.6458462355127	26.94879879034528	25.802280971262658
27	22.74255426958249	26.460392655208192	27.564283014163575	23.23277006104574
28	21.009506727900074	25.959318925788004	29.53459660749761	23.496577738814313
29	23.05502159523216	26.341156410607	28.090289786299692	22.513532207861147
30	25.64765589183885	24.740414853659125	27.719511312362766	21.892417942139257
31	26.917431414025238	25.201676143903875	25.346247577071253	22.53464486499964
32	27.89364046552404	25.877884391110967	26.764615990927588	19.463859152437408
33	27.629028496054946	23.588870211448288	25.369572036386156	23.412529256110606
34	23.3588427851013	26.39826112134349	29.57702299469972	20.665873098855492
35	22.259778176349	27.33686953584326	27.98110718795493	22.422245099852812
36	27.49249997989271	27.455301488743938	24.155091568610104	20.897106962753252
37	25.08907530583192	26.259319730079707	27.391561371478208	21.260043592610167
38	24.835522347244897	26.4103254968512	25.646851600138337	23.107300555765566
39	23.926672725664144	23.943964997225194	28.039217263317063	24.090145013793602
40	27.102418505143444	21.900460859144395	29.56898007769458	21.42814055801758
41	21.27472191614455	25.05589827318572	27.117096828677823	26.552282981991908
42	23.75194035372749	23.052005501355232	29.65765323767624	23.538400907241037
43	23.857302566494816	23.45998246644093	27.15570283030249	25.527012136761762
44	24.233510009410214	23.237394738323694	28.179365092131615	24.349730160134477
45	22.899994369958097	23.728615894412584	26.577216024707838	26.794173710921477
46	24.507974552210595	25.334585347413803	26.60757803640224	23.54986206397336
47	21.20675926745112	25.188003184995132	31.00303217971094	22.60220536784281
48	24.014943739795548	24.60549492089791	27.890021152871725	23.489540186434816
49	24.629020453137944	22.361118930613756	30.06784200493835	22.94201861130995
50	22.180153297998118	25.137332807862755	28.643039257477902	24.03947463666122
51	22.643023171643893	23.654419985040175	26.103689286030257	27.59886755728568
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	295.0
1	589.0
2	883.0
3	1645.0
4	2407.0
5	1656.0
6	905.0
7	936.0
8	967.0
9	1017.0
10	1067.0
11	1042.5
12	1018.0
13	1017.5
14	1017.0
15	1070.5
16	1124.0
17	1205.5
18	1287.0
19	1178.5
20	1070.0
21	1750.0
22	2430.0
23	2223.0
24	2016.0
25	2501.5
26	3125.0
27	3263.0
28	3722.0
29	4181.0
30	5080.5
31	5980.0
32	6956.0
33	7932.0
34	9132.5
35	10333.0
36	11004.5
37	11676.0
38	12604.0
39	13532.0
40	15479.0
41	17426.0
42	20978.5
43	24531.0
44	27436.0
45	30341.0
46	41127.0
47	51913.0
48	48496.5
49	45080.0
50	45557.0
51	46034.0
52	41802.0
53	37570.0
54	33984.5
55	30399.0
56	29713.5
57	29028.0
58	27399.0
59	25770.0
60	23738.0
61	21706.0
62	20076.5
63	18447.0
64	16284.0
65	14121.0
66	11920.0
67	9719.0
68	8249.5
69	6780.0
70	5597.0
71	4414.0
72	3697.0
73	2980.0
74	2561.5
75	1803.0
76	1463.0
77	1019.0
78	575.0
79	460.5
80	346.0
81	227.5
82	109.0
83	74.0
84	39.0
85	25.0
86	11.0
87	8.5
88	6.0
89	5.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.87624871624951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52913750625135	23.01180186250576
2	9.803592337409697	6.053963106451594
3	3.6271834109940846	3.359814514119228
4	1.8370418002958953	2.268838381123313
5	1.170934261281696	1.8077028740855765
6	0.7949430877171368	1.47269162949706
7	0.633371776323199	1.368930114692549
8	0.5178800219443694	1.2792153890184883
9	0.4025019925344665	1.1184976467252172
>10	6.36538070966494	46.27310418187843
>50	0.2638303298061569	4.937458599682366
>100	0.0444070852148977	2.3934182863654057
>500	0.005224362966458553	1.1291611123543617
>1k	0.004571317595651233	3.5254023015006535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	3953	0.7948412730329035	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	3756	0.7552299067825919	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	3736	0.7512084482800222	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1790	0.35992053597998924	RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1431	0.2877353558588629	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1412	0.28391497028142165	Illumina PCR Primer Index 5 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	1406	0.2827085327306507	Illumina PCR Primer Index 5 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	922	0.18538923696846374	Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT	855	0.1719173509848552	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	753	0.15140791262174966	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	744	0.14959825629559328	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	719	0.14457143316738116	RNA PCR Primer, Index 33 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA	556	0.11179654637143799	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC	542	0.10898152541963918	RNA PCR Primer, Index 33 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	509	0.10234611889039917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.010729251284856E-4	0.0	0.0	0.19463859152437407	0.0
2	2.010729251284856E-4	0.0	0.0	1.0377373665881142	0.0
3	2.010729251284856E-4	0.0	0.0	1.4101244239260695	0.0
4	2.010729251284856E-4	0.0	0.0	1.9343215397360314	0.0
5	2.010729251284856E-4	0.0	0.0	3.6834549154287277	0.0
6	2.010729251284856E-4	0.0	0.0	4.639154528564419	0.0
7	2.010729251284856E-4	0.0	0.0	5.428164686768597	0.0
8	2.010729251284856E-4	0.0	0.0	6.419253134726903	0.0
9	2.010729251284856E-4	0.0	0.0	6.816975380631047	0.0
10	2.010729251284856E-4	0.0	0.0	8.34352102820651	0.0
11	4.021458502569712E-4	0.0	0.0	9.537894203469714	0.0
12	4.021458502569712E-4	0.0	0.0	11.286424360387025	0.0
13	4.021458502569712E-4	0.0	0.0	11.794133496336451	0.0
14	4.021458502569712E-4	0.0	0.0	12.052914350976812	0.0
15	4.021458502569712E-4	0.0	0.0	12.473558910345604	0.0
16	4.021458502569712E-4	0.0	0.0	13.15921758503374	0.0
17	4.021458502569712E-4	0.0	0.0	13.859152437405998	0.0
18	4.021458502569712E-4	0.0	0.0	14.735830390966196	0.0
19	4.021458502569712E-4	0.0	0.0	15.395349585387628	0.0
20	4.021458502569712E-4	0.0	0.0	15.908487690315523	0.0
21	4.021458502569712E-4	0.0	0.0	16.53663950841691	0.0
22	4.021458502569712E-4	0.0	0.0	17.183491108555252	0.0
23	4.021458502569712E-4	0.0	0.0	17.738653454835	0.0
24	4.021458502569712E-4	0.0	0.0	18.20474049528283	0.0
25	6.032187753854568E-4	0.0	0.0	18.623374325400338	0.0
26	6.032187753854568E-4	0.0	0.0	19.077598063265587	0.0
27	8.042917005139424E-4	0.0	0.0	19.6064198563535	0.0
28	0.001005364625642428	0.0	0.0	19.993887383076093	0.0
29	0.001005364625642428	0.0	0.0	20.447507902165956	0.0
30	0.001005364625642428	0.0	0.0	21.00910458204982	0.0
31	0.001005364625642428	0.0	0.0	21.434775964546823	0.0
32	0.001005364625642428	0.0	0.0	21.852806575888945	0.0
33	0.001005364625642428	0.0	0.0	22.29918846967418	0.0
34	0.001005364625642428	0.0	0.0	22.772715208351766	0.0
35	0.001005364625642428	0.0	0.0	23.251670916007818	0.0
36	0.001005364625642428	0.0	0.0	23.674125131702766	0.0
37	0.001005364625642428	0.0	0.0	24.150869037182407	0.0
38	0.001005364625642428	0.0	0.0	24.68109834074622	0.0
39	0.001005364625642428	0.0	0.0	25.541891533221268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	35	1.2100645E-7	45.000004	1
GTCGATG	25	3.887603E-5	45.0	1
GTTTACG	20	7.029423E-4	45.0	1
TTCACGG	50	2.1827873E-11	45.0	2
ACTTAGC	25	3.887603E-5	45.0	39
GACCAAT	20	7.029423E-4	45.0	24
CGGATAG	20	7.029423E-4	45.0	18
ATAGTAG	20	7.029423E-4	45.0	1
ATCGGAC	20	7.029423E-4	45.0	31
TCGACAA	25	3.887603E-5	45.0	19
TATCGTG	25	3.887603E-5	45.0	1
TAGTCCG	20	7.029423E-4	45.0	2
GTATACG	30	2.1630094E-6	44.999996	1
CGATGAA	260	0.0	42.40385	19
TACGAAT	70	0.0	41.785717	12
CGGGTAT	55	6.002665E-11	40.909092	6
TATTGCG	50	1.0786607E-9	40.5	1
TACACGG	45	1.9244908E-8	40.000004	2
CCGATGA	280	0.0	39.375004	18
CGTATGG	40	3.4529512E-7	39.375	2
>>END_MODULE