##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935572.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668995
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.183748757464556	33.0	31.0	34.0	30.0	34.0
2	32.200272049865845	33.0	31.0	34.0	30.0	34.0
3	32.1976905656993	34.0	31.0	34.0	30.0	34.0
4	35.84438000284008	37.0	35.0	37.0	35.0	37.0
5	35.92433276780843	37.0	35.0	37.0	35.0	37.0
6	35.86892278716582	37.0	35.0	37.0	35.0	37.0
7	36.12958093857204	37.0	35.0	37.0	35.0	37.0
8	35.94300555310578	37.0	35.0	37.0	35.0	37.0
9	37.773645542941274	39.0	38.0	39.0	35.0	39.0
10	37.4140060837525	39.0	37.0	39.0	34.0	39.0
11	37.39694766029641	39.0	37.0	39.0	35.0	39.0
12	37.31870940739467	39.0	37.0	39.0	35.0	39.0
13	37.29049843421849	39.0	37.0	39.0	34.0	39.0
14	38.43961913018782	40.0	38.0	41.0	34.0	41.0
15	38.55076196384128	40.0	38.0	41.0	35.0	41.0
16	38.328347745498846	40.0	37.0	41.0	34.0	41.0
17	38.37949162549795	40.0	38.0	41.0	34.0	41.0
18	38.28752681260697	40.0	37.0	41.0	34.0	41.0
19	38.16470078251706	40.0	37.0	41.0	34.0	41.0
20	38.31181996875911	40.0	37.0	41.0	34.0	41.0
21	38.22727524122004	40.0	37.0	41.0	34.0	41.0
22	38.325512148820245	40.0	37.0	41.0	34.0	41.0
23	38.34414905941001	40.0	37.0	41.0	34.0	41.0
24	38.28039372491573	40.0	37.0	41.0	34.0	41.0
25	38.027645946531734	40.0	36.0	41.0	34.0	41.0
26	38.11721014357357	40.0	37.0	41.0	34.0	41.0
27	38.12734026412753	40.0	37.0	41.0	34.0	41.0
28	38.017690715177245	40.0	36.0	41.0	34.0	41.0
29	37.99891030575714	40.0	36.0	41.0	34.0	41.0
30	37.830581693435676	40.0	36.0	41.0	33.0	41.0
31	37.78582052182752	40.0	36.0	41.0	33.0	41.0
32	37.72260031838803	40.0	36.0	41.0	33.0	41.0
33	37.658384591813096	40.0	36.0	41.0	33.0	41.0
34	37.55426124261018	40.0	36.0	41.0	33.0	41.0
35	37.45970448209628	40.0	36.0	41.0	33.0	41.0
36	37.37342282079836	40.0	35.0	41.0	33.0	41.0
37	37.35996681589548	40.0	35.0	41.0	33.0	41.0
38	37.23684481946801	40.0	35.0	41.0	33.0	41.0
39	37.17363208992593	40.0	35.0	41.0	32.0	41.0
40	37.09158962324083	40.0	35.0	41.0	32.0	41.0
41	36.959935425526346	39.0	35.0	41.0	32.0	41.0
42	36.95695633001741	39.0	35.0	41.0	32.0	41.0
43	36.898854251526544	39.0	35.0	41.0	32.0	41.0
44	36.78640946494368	39.0	35.0	41.0	31.0	41.0
45	36.756960814355864	39.0	35.0	41.0	31.0	41.0
46	36.6153155105793	39.0	35.0	41.0	31.0	41.0
47	36.56817315525527	39.0	35.0	41.0	31.0	41.0
48	36.480670259120025	39.0	35.0	41.0	31.0	41.0
49	36.43286422170569	39.0	35.0	41.0	31.0	41.0
50	36.312908168222485	39.0	35.0	41.0	31.0	41.0
51	35.19730042825432	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	16.0
10	15.0
11	15.0
12	13.0
13	20.0
14	30.0
15	27.0
16	51.0
17	71.0
18	171.0
19	261.0
20	465.0
21	741.0
22	1164.0
23	1678.0
24	2443.0
25	3567.0
26	4954.0
27	6102.0
28	6648.0
29	7210.0
30	8469.0
31	10888.0
32	14116.0
33	20127.0
34	38683.0
35	52659.0
36	44825.0
37	68111.0
38	127903.0
39	247381.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09419352909962	20.582216608494832	23.25548023527829	13.068109627127258
2	32.70622351437604	24.58030329075703	24.578659033326108	18.13481416154082
3	32.33716246010807	23.571775573808473	27.146839662478794	16.94422230360466
4	28.53145389726381	25.13426856702965	27.050575863795697	19.28370167191085
5	26.307222027070452	29.135643764153695	25.010799781762195	19.546334427013655
6	25.707367020680273	34.297416273664226	24.823503912585295	15.171712793070203
7	79.01807935784274	6.043542926329793	10.113229545811254	4.825148170016218
8	80.06427551775424	6.170300226459092	8.997675617904468	4.767748637882196
9	73.52252258985493	8.116503112878274	11.390219657844975	6.97075463942182
10	39.11583793600849	28.12218327491237	18.47861344255189	14.283365346527255
11	30.597986532036863	25.27208723533061	25.68644010792308	18.44348612470945
12	28.40051121458307	22.30838795506693	29.449397977563358	19.841702852786643
13	25.044581798070244	23.70525938160973	31.080800304935014	20.16935851538502
14	20.255607291534318	27.54325518127938	30.23266242647553	21.96847510071077
15	19.935126570452695	26.224859677576067	33.8619870103663	19.978026741604946
16	23.38986091076914	25.497350503366988	31.190816074858557	19.921972511005315
17	22.50121450832966	25.031577216571126	29.409487365376425	23.057720909722793
18	23.46220823772973	25.690326534577984	29.66270300973849	21.184762217953796
19	24.45952510855836	27.03114373052116	27.50723099574735	21.002100165173132
20	26.329643719310308	26.12530736403112	27.633539862031853	19.911509054626716
21	25.0003363253836	26.81978191167348	28.435339576528975	19.74454218641395
22	22.627822330510693	25.725603330368685	28.646701395376645	22.999872943743974
23	22.80301048587807	26.658196249598276	28.648943564600632	21.88984969992302
24	23.006001539622865	24.625146675236735	29.80605236212528	22.56279942301512
25	22.084918422409732	27.33219231832824	27.574197116570375	23.00869214269165
26	21.28924730379151	27.851478710603217	28.234441214059895	22.62483277154538
27	22.000164425743094	27.287199455900268	28.886165068498272	21.82647104985837
28	19.87922181780133	28.07165972839857	30.105606170449704	21.9435122833504
29	22.387760745596005	27.265076719556948	28.073005029932958	22.27415750491409
30	22.499869206795267	26.502290749557172	29.432058535564543	21.56578150808302
31	22.721246048176745	26.862682082825728	28.172258387581373	22.243813481416154
32	23.475511775125373	26.99003729474809	28.00379673988595	21.530654190240583
33	23.657426438164713	25.941897921509128	28.026367910074068	22.374307730252095
34	21.550385279411653	25.823212430586178	30.05044880753967	22.5759534824625
35	21.013012055396526	26.18883549204404	28.970769587216644	23.82738286534279
36	21.410623397783244	28.440870260614805	27.89647157303119	22.252034768570766
37	21.09911135359756	27.17778159776979	29.124283440085502	22.59882360854715
38	21.508381975949	27.029349995141967	27.425616035994288	24.036651992914745
39	23.094940918840948	24.92111301280279	27.762987765229934	24.22095830312633
40	22.88372857794154	24.5747726066712	29.66031136256624	22.881187452821024
41	19.740207325914245	26.377327184807058	28.92846732785746	24.953998161421236
42	20.619885051457786	26.460885357887577	28.501109873765873	24.418119716888768
43	21.640968916060658	25.436811934319394	28.824430675864544	24.09778847375541
44	22.159059484749513	25.4507133835081	28.519196705506022	23.87103042623637
45	21.619892525355198	25.165509458217176	27.76029716216115	25.454300854266478
46	21.525721417947818	26.726208716059162	27.842958467552076	23.905111398440944
47	20.898810902921547	25.49361355466035	30.556581140367268	23.050994402050836
48	21.101353522821544	24.67656708944013	29.314867824124246	24.90721156361408
49	20.972354053468262	24.6814998617329	30.059417484435606	24.28672860036323
50	20.02107639070546	24.2663995993991	29.997533613853616	25.714990396041827
51	19.805977623151144	24.409449995889357	28.278238252901737	27.50633412805776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	321.0
1	739.0
2	1157.0
3	3285.5
4	5414.0
5	3608.0
6	1802.0
7	1736.0
8	1670.0
9	1676.5
10	1683.0
11	1768.5
12	1854.0
13	1815.0
14	1776.0
15	1744.0
16	1712.0
17	1720.0
18	1728.0
19	2067.5
20	2407.0
21	2444.5
22	2482.0
23	2727.0
24	2972.0
25	3862.0
26	5527.0
27	6302.0
28	7538.5
29	8775.0
30	10265.5
31	11756.0
32	13816.0
33	15876.0
34	17345.0
35	18814.0
36	20180.0
37	21546.0
38	22948.0
39	24350.0
40	27224.5
41	30099.0
42	33339.0
43	36579.0
44	40155.0
45	43731.0
46	50996.5
47	58262.0
48	59541.0
49	60820.0
50	59056.0
51	57292.0
52	51287.5
53	45283.0
54	40862.5
55	36442.0
56	35498.5
57	34555.0
58	32531.5
59	30508.0
60	29454.0
61	28400.0
62	26610.5
63	24821.0
64	20015.5
65	15210.0
66	12557.0
67	9904.0
68	8216.0
69	6528.0
70	5534.0
71	4540.0
72	3637.0
73	2734.0
74	2244.0
75	1498.0
76	1242.0
77	910.5
78	579.0
79	404.0
80	229.0
81	175.5
82	122.0
83	109.0
84	96.0
85	79.5
86	63.0
87	43.0
88	23.0
89	21.0
90	19.0
91	13.5
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	668995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.212812958444278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24672325631812	22.1298954956207
2	9.287947363513911	5.61230032923443
3	3.298965999197097	2.990131280700274
4	1.779676934682728	2.1507618541610687
5	1.1817372706336855	1.7851803561838995
6	0.9213606296372381	1.67021378223026
7	0.7940166675682833	1.6792633944189443
8	0.6748653890758894	1.6311665417822057
9	0.5930744150880717	1.6126601736145192
>10	8.028291762880825	51.5235278813549
>50	0.1396622815029258	2.738829505121232
>100	0.04970187953816205	2.818866575466409
>500	9.94037590763241E-4	0.2362069637336811
>1k	0.0029821127722897234	1.4209958663775106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3180	0.4753398754848691	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1480	0.2212273634332095	TruSeq Adapter, Index 22 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1291	0.1929760312109956	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	1246	0.1862495235390399	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	1191	0.17802823638442739	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1075	0.16068879438560826	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	824	0.12316982937092207	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCC	749	0.1119589832509959	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4947794826568211E-4	0.0	0.0	0.1295973811463464	0.0
2	1.4947794826568211E-4	0.0	0.0	0.6597956636447209	0.0
3	1.4947794826568211E-4	0.0	0.0	0.9560609571073028	0.0
4	1.4947794826568211E-4	0.0	0.0	1.3668263589413971	0.0
5	1.4947794826568211E-4	0.0	0.0	2.4814834191585886	0.0
6	1.4947794826568211E-4	0.0	0.0	3.250547462985523	0.0
7	1.4947794826568211E-4	0.0	0.0	3.907054611768399	0.0
8	1.4947794826568211E-4	0.0	0.0	4.798541095224927	0.0
9	1.4947794826568211E-4	0.0	0.0	5.1835962899573245	0.0
10	1.4947794826568211E-4	0.0	0.0	6.1126017384285385	0.0
11	2.9895589653136423E-4	0.0	0.0	7.161637979357096	0.0
12	2.9895589653136423E-4	0.0	0.0	8.109627127258051	0.0
13	2.9895589653136423E-4	0.0	0.0	8.469570026681813	0.0
14	2.9895589653136423E-4	0.0	0.0	8.620393276481886	0.0
15	2.9895589653136423E-4	0.0	0.0	8.912622665341296	0.0
16	2.9895589653136423E-4	0.0	0.0	9.442073558098341	0.0
17	2.9895589653136423E-4	0.0	0.0	10.072422065934722	0.0
18	2.9895589653136423E-4	0.0	0.0	10.765401834094424	0.0
19	4.484338447970463E-4	0.0	0.0	11.21249037735708	0.0
20	7.473897413284105E-4	0.0	0.0	11.651656589361654	0.0
21	7.473897413284105E-4	0.0	0.0	12.163020650378552	0.0
22	7.473897413284105E-4	0.0	0.0	12.728495728667628	0.0
23	7.473897413284105E-4	0.0	0.0	13.272894416251242	0.0
24	7.473897413284105E-4	0.0	0.0	13.71370488568674	0.0
25	7.473897413284105E-4	0.0	0.0	14.132093662882383	0.0
26	7.473897413284105E-4	0.0	0.0	14.514757210442529	0.0
27	7.473897413284105E-4	0.0	0.0	14.91042533950179	0.0
28	7.473897413284105E-4	0.0	0.0	15.308933549578098	0.0
29	7.473897413284105E-4	0.0	0.0	15.708637583240533	0.0
30	0.0010463456378597747	0.0	0.0	16.20101794482769	0.0
31	0.001195823586125457	0.0	0.0	16.648704399883407	0.0
32	0.001195823586125457	0.0	0.0	17.066345787337724	0.0
33	0.001195823586125457	0.0	0.0	17.503419308066576	0.0
34	0.001195823586125457	0.0	0.0	17.908803503763107	0.0
35	0.001195823586125457	0.0	0.0	18.341990597837054	0.0
36	0.001195823586125457	0.0	0.0	18.76620901501506	0.0
37	0.001345301534391139	0.0	0.0	19.181010321452327	0.0
38	0.001345301534391139	0.0	0.0	19.620176533456902	0.0
39	0.001345301534391139	0.0	0.0	20.069656723891807	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.031169E-4	45.000004	9
CGACGTA	20	7.031169E-4	45.000004	33
CGCATCG	20	7.031169E-4	45.000004	21
CGCGACT	20	7.031169E-4	45.000004	23
ATCGTAG	20	7.031169E-4	45.000004	1
TTGCGTG	20	7.031169E-4	45.000004	1
CCGAATA	20	7.031169E-4	45.000004	39
CCGCGAC	20	7.031169E-4	45.000004	22
CGACTCG	20	7.031169E-4	45.000004	25
CTCCGAC	25	3.889051E-5	45.0	20
GCCGATA	25	3.889051E-5	45.0	9
AAGTCGG	25	3.889051E-5	45.0	2
GTTCGTA	30	2.1641372E-6	44.999996	30
AACACGT	155	0.0	42.096775	41
GCGAGAC	145	0.0	41.89655	21
GCTACGA	65	0.0	41.53846	10
GTCAAGC	165	0.0	40.909092	16
ACGGGCC	50	1.0804797E-9	40.5	5
TTACGCG	45	1.9263098E-8	40.0	1
TGCACGG	90	0.0	40.0	2
>>END_MODULE