##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935571.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608390
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20625092457141	33.0	31.0	34.0	30.0	34.0
2	32.21419155475928	34.0	31.0	34.0	30.0	34.0
3	32.228171074475256	34.0	31.0	34.0	30.0	34.0
4	35.86571278291885	37.0	35.0	37.0	35.0	37.0
5	35.938069330528116	37.0	35.0	37.0	35.0	37.0
6	35.874605105277865	37.0	35.0	37.0	35.0	37.0
7	36.111648777922056	37.0	35.0	37.0	35.0	37.0
8	35.90577261296208	37.0	35.0	37.0	35.0	37.0
9	37.73970150725686	39.0	38.0	39.0	35.0	39.0
10	37.40998208386068	39.0	37.0	39.0	34.0	39.0
11	37.43225398182087	39.0	37.0	39.0	35.0	39.0
12	37.31260375745821	39.0	37.0	39.0	35.0	39.0
13	37.25031640888246	39.0	37.0	39.0	34.0	39.0
14	38.41332369039596	40.0	38.0	41.0	34.0	41.0
15	38.538953631716495	40.0	38.0	41.0	34.0	41.0
16	38.32076628478443	40.0	37.0	41.0	34.0	41.0
17	38.380277453607064	40.0	37.0	41.0	34.0	41.0
18	38.297508177320466	40.0	37.0	41.0	34.0	41.0
19	38.160663390259536	40.0	37.0	41.0	34.0	41.0
20	38.31255280330051	40.0	37.0	41.0	34.0	41.0
21	38.23130228964973	40.0	37.0	41.0	34.0	41.0
22	38.292935452587976	40.0	37.0	41.0	34.0	41.0
23	38.330529758871776	40.0	37.0	41.0	34.0	41.0
24	38.27923535889808	40.0	37.0	41.0	34.0	41.0
25	38.00739164023077	40.0	36.0	41.0	34.0	41.0
26	38.1080244579957	40.0	37.0	41.0	34.0	41.0
27	38.10839428655961	40.0	37.0	41.0	34.0	41.0
28	37.981860319860616	40.0	36.0	41.0	34.0	41.0
29	37.964565492529466	40.0	36.0	41.0	34.0	41.0
30	37.81292591922944	40.0	36.0	41.0	33.0	41.0
31	37.785667088545175	40.0	36.0	41.0	33.0	41.0
32	37.743136803694995	40.0	36.0	41.0	33.0	41.0
33	37.70708920264962	40.0	36.0	41.0	33.0	41.0
34	37.63759101891878	40.0	36.0	41.0	33.0	41.0
35	37.55952431828268	40.0	36.0	41.0	33.0	41.0
36	37.445786419895136	40.0	35.0	41.0	33.0	41.0
37	37.40241456960174	40.0	35.0	41.0	33.0	41.0
38	37.28429790101744	40.0	35.0	41.0	33.0	41.0
39	37.22285540524992	40.0	35.0	41.0	33.0	41.0
40	37.11206134223114	40.0	35.0	41.0	32.0	41.0
41	36.979935567645754	39.0	35.0	41.0	32.0	41.0
42	36.983122668025445	39.0	35.0	41.0	32.0	41.0
43	36.89920117030194	39.0	35.0	41.0	32.0	41.0
44	36.79198376041684	39.0	35.0	41.0	32.0	41.0
45	36.76177945068131	39.0	35.0	41.0	31.0	41.0
46	36.61922451059353	39.0	35.0	41.0	31.0	41.0
47	36.56986965597725	39.0	35.0	41.0	31.0	41.0
48	36.457499301434936	39.0	35.0	41.0	31.0	41.0
49	36.389381811009386	39.0	35.0	41.0	31.0	41.0
50	36.2725488584625	38.0	35.0	41.0	31.0	41.0
51	35.140667992570556	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	13.0
11	10.0
12	13.0
13	22.0
14	22.0
15	33.0
16	52.0
17	83.0
18	153.0
19	264.0
20	454.0
21	725.0
22	1144.0
23	1564.0
24	2285.0
25	3013.0
26	4056.0
27	5008.0
28	5623.0
29	6666.0
30	7930.0
31	9948.0
32	12840.0
33	17996.0
34	35599.0
35	50037.0
36	40784.0
37	63241.0
38	114507.0
39	224112.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.763868571146794	19.940827429773663	23.74200759381318	12.553296405266359
2	31.77928631305577	25.388977465112838	25.025066158220877	17.806670063610515
3	33.440556222160126	23.940728808823287	25.47559953319417	17.143115435822416
4	29.98126201942833	24.7956080803432	25.53279968441296	19.690330215815514
5	26.153782935288223	30.482913920347144	23.95174148161541	19.411561662749225
6	25.756176137017373	36.12781275168888	23.73247423527671	14.383536876017027
7	78.74603461595359	7.226121402389913	9.36751097158073	4.6603330100757745
8	79.30044872532422	7.515409523496441	8.501454659018064	4.682687092161278
9	72.48837094626802	9.630335804336035	10.815430891369022	7.065862358026924
10	39.984385016190274	26.579661072667204	18.814576176465753	14.621377734676768
11	32.06660201515476	26.87535955554825	22.528476799421423	18.529561629875573
12	28.555696181725537	24.05069116849389	28.161376748467266	19.232235901313302
13	25.206035602163084	25.941912260227813	29.051923930373608	19.80012820723549
14	20.68985354788869	29.56080803432009	27.865020792583707	21.884317625207515
15	19.926363023718338	26.535774749749336	32.730649747694734	20.807212478837588
16	22.99594010420947	25.08456746494847	31.07266720360295	20.84682522723911
17	22.816121238021665	25.323230164861354	27.636877660711058	24.223770936405923
18	24.173967356465425	25.785104949128023	28.484689097453934	21.55623859695261
19	25.488420256743204	27.037755387169415	25.89802593730995	21.575798418777428
20	26.49895626160851	25.66363681191341	27.64936964775884	20.188037278719243
21	25.828826903795267	27.12733608376206	27.043015171189534	20.000821841253146
22	23.574680714673153	25.415112016962805	27.293019280395796	23.717187987968245
23	22.898962836338534	27.066026726277553	27.775604464241688	22.259405973142226
24	22.133828629661895	25.661993129407122	29.30307861733428	22.901099623596707
25	22.378572954848043	27.731718141323825	27.205575371061325	22.68413353276681
26	21.963871858511812	28.342674928910732	26.573415079143313	23.120038133434146
27	23.09275300382978	27.8461184437614	26.845608902184452	22.215519650224362
28	21.24656881276813	28.804385344926775	28.936208681930996	21.0128371603741
29	23.301007577376353	26.24007626686829	27.897565706208187	22.561350449547167
30	22.385147684873193	26.94751721757425	27.444566807475468	23.222768290077088
31	23.935140288301913	28.646263087821954	25.150314765199955	22.26828185867618
32	24.44698302075971	28.26410690511021	25.508801919821167	21.780108154308913
33	22.301319877052546	27.784644718026264	25.82586827528395	24.088167129637238
34	21.926231529117835	28.333634675126152	27.042850802938904	22.697282992817108
35	23.19071648120449	25.853153404888314	26.801393842765332	24.154736271141868
36	22.76040040105853	28.834464734791826	26.721017768207894	21.684117095941748
37	21.800161080885616	29.24817962162429	27.770509048472196	21.1811502490179
38	22.121994115616626	28.82805437301731	26.01620670951199	23.033744801854077
39	23.206495833264846	26.033629744078635	27.62323509590888	23.136639326747645
40	22.949259521030918	26.127484015187623	28.85747628987985	22.065780173901608
41	20.60684758132119	27.096434852643863	28.637058465786748	23.659659100248195
42	22.382024688111244	26.522954026200303	27.965121057216585	23.129900228471868
43	23.027005703578297	26.117128815398015	28.067358109107644	22.78850737191604
44	22.58436200463518	27.235161656174494	26.304508621114746	23.87596771807558
45	22.4642088134256	26.577195548907774	26.247801574647838	24.71079406301879
46	22.318414175117933	27.783001035519977	26.822926083597693	23.075658705764397
47	21.940038462170648	26.33376616972666	29.081345847236147	22.64484952086655
48	21.726524104603957	26.39408931770739	28.32377258008843	23.555613997600226
49	21.919328062591433	25.503542135801048	29.996548266736795	22.580581534870724
50	21.67326879140025	25.084731833199097	29.341376419730764	23.900622955669885
51	20.739985864330446	25.956705402784397	27.16464767665478	26.13866105623038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	845.5
2	1408.0
3	2149.5
4	2891.0
5	2076.5
6	1262.0
7	1224.0
8	1186.0
9	1220.0
10	1254.0
11	1256.0
12	1258.0
13	1420.0
14	1582.0
15	1477.0
16	1372.0
17	1379.5
18	1387.0
19	1513.0
20	1639.0
21	2012.5
22	2386.0
23	2722.0
24	3058.0
25	3818.0
26	5598.5
27	6619.0
28	7919.0
29	9219.0
30	10306.5
31	11394.0
32	13079.0
33	14764.0
34	15703.5
35	16643.0
36	18118.0
37	19593.0
38	20606.0
39	21619.0
40	24413.5
41	27208.0
42	29990.5
43	32773.0
44	36717.0
45	40661.0
46	47110.0
47	53559.0
48	53645.0
49	53731.0
50	51331.5
51	48932.0
52	44752.5
53	40573.0
54	36453.5
55	32334.0
56	31331.5
57	30329.0
58	29617.0
59	28905.0
60	27103.0
61	25301.0
62	23235.5
63	21170.0
64	18343.0
65	15516.0
66	13565.0
67	11614.0
68	9476.0
69	7338.0
70	6082.5
71	4827.0
72	4103.0
73	3379.0
74	2734.0
75	1844.5
76	1600.0
77	1061.0
78	522.0
79	358.5
80	195.0
81	190.5
82	186.0
83	164.5
84	143.0
85	112.5
86	82.0
87	49.5
88	17.0
89	12.0
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.926674574994284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93301449418294	19.168372071390174
2	9.436158264891162	4.892964091439517
3	3.3349448340461914	2.5939208831362177
4	1.6036157526947925	1.663056950538096
5	1.053533168087623	1.365730580148528
6	0.6979866653379521	1.0857883877941517
7	0.5703956041006976	1.0351922844568242
8	0.4976198154505015	1.032130161380309
9	0.4072322570059754	0.9502360383450923
>10	7.7466392084908176	50.496729978304344
>50	0.6367227065645182	10.025700754188396
>100	0.0732231112549196	3.5386768320948176
>500	0.005093781652516146	0.8617229456771295
>1k	0.0038203362393871097	1.2897780411064008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1529	0.2513190552112954	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1416	0.23274544289025134	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	1301	0.21384309406794985	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	1265	0.20792583704531634	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	1165	0.19148901198244547	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	1137	0.18688670096484164	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	903	0.14842453031772382	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	710	0.11670145794638308	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	695	0.11423593418695245	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	628	0.10322326139482897	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	624	0.10256578839231414	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.931047518861257E-4	0.0	0.0	0.1208106642121008	0.0
2	4.931047518861257E-4	0.0	0.0	0.6222981968802906	0.0
3	4.931047518861257E-4	0.0	0.0	0.8653988395601505	0.0
4	4.931047518861257E-4	0.0	0.0	1.181643353769786	0.0
5	4.931047518861257E-4	0.0	0.0	2.0830388402176236	0.0
6	4.931047518861257E-4	0.0	0.0	2.6137839214977237	0.0
7	4.931047518861257E-4	0.0	0.0	3.0769736517694244	0.0
8	4.931047518861257E-4	0.0	0.0	3.815480201844212	0.0
9	4.931047518861257E-4	0.0	0.0	4.092276335902957	0.0
10	4.931047518861257E-4	0.0	0.0	4.894393398971054	0.0
11	4.931047518861257E-4	0.0	0.0	5.729219743914266	0.0
12	4.931047518861257E-4	0.0	0.0	6.557800095333586	0.0
13	4.931047518861257E-4	0.0	0.0	6.894755009122438	0.0
14	4.931047518861257E-4	0.0	0.0	7.040385279179474	0.0
15	6.574730025148343E-4	0.0	0.0	7.303210111934779	0.0
16	6.574730025148343E-4	0.0	0.0	7.746840020381663	0.0
17	6.574730025148343E-4	0.0	0.0	8.29057019346143	0.0
18	6.574730025148343E-4	0.0	0.0	8.920264961620013	0.0
19	6.574730025148343E-4	0.0	0.0	9.2642877101859	0.0
20	6.574730025148343E-4	0.0	0.0	9.620309341047683	0.0
21	6.574730025148343E-4	0.0	0.0	10.061473725735137	0.0
22	6.574730025148343E-4	0.0	0.0	10.512664573710943	0.0
23	8.218412531435428E-4	0.0	0.0	10.953500221897139	0.0
24	8.218412531435428E-4	0.0	0.0	11.334341458603856	0.0
25	8.218412531435428E-4	0.0	0.0	11.68378835944049	0.0
26	8.218412531435428E-4	0.0	0.0	11.999046664146354	0.0
27	8.218412531435428E-4	0.0	0.0	12.316277387859762	0.0
28	8.218412531435428E-4	0.0	0.0	12.64994493663604	0.0
29	8.218412531435428E-4	0.0	0.0	13.007774618254738	0.0
30	8.218412531435428E-4	0.0	0.0	13.442035536415785	0.0
31	8.218412531435428E-4	0.0	0.0	13.845888328210522	0.0
32	8.218412531435428E-4	0.0	0.0	14.220976676145236	0.0
33	8.218412531435428E-4	0.0	0.0	14.61250184914282	0.0
34	8.218412531435428E-4	0.0	0.0	14.964414273738885	0.0
35	8.218412531435428E-4	0.0	0.0	15.361692335508474	0.0
36	8.218412531435428E-4	0.0	0.0	15.72872663916238	0.0
37	8.218412531435428E-4	0.0	0.0	16.089843685793653	0.0
38	8.218412531435428E-4	0.0	0.0	16.46049409096139	0.0
39	8.218412531435428E-4	0.0	0.0	16.83706175315176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGTA	30	2.1638098E-6	45.000004	7
ACTACGG	45	3.8380676E-10	45.000004	2
GCGCGTA	20	7.0306653E-4	45.0	22
CGTTGAT	25	3.8886334E-5	45.0	25
ACGTGCG	25	3.8886334E-5	45.0	1
CGAATCG	20	7.0306653E-4	45.0	32
GCGATAC	20	7.0306653E-4	45.0	9
TCGACGG	20	7.0306653E-4	45.0	2
AATCGTC	20	7.0306653E-4	45.0	34
CTTACCG	20	7.0306653E-4	45.0	10
TCGTGTA	155	0.0	43.548386	17
TATGGGT	70	0.0	41.785713	4
CGTAAGG	70	0.0	41.785713	2
CGTATGG	65	0.0	41.53846	2
CCTCGTG	170	0.0	41.02941	15
CGGGTAC	55	6.002665E-11	40.909092	6
TTCGAAT	55	6.002665E-11	40.909092	9
TAGGGCG	140	0.0	40.17857	5
AGTACGG	85	0.0	39.705883	2
ACGTATG	40	3.454643E-7	39.375	1
>>END_MODULE