FastQCFastQC Report
Sat 14 Jan 2017
SRR2935568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511441
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.5482548329132784No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC15190.2970039554904671No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC12740.24910009170168212No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG11330.2215309292762997No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC6000.11731558478886128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG207.029611E-445.037
CGGTCTA406.8030204E-945.031
TAGGACG207.029611E-445.01
TATCGAG351.2101555E-745.01
GCGATAT302.1631295E-644.9999969
CGTAAGG1350.043.3333322
CGTTTTT11350.040.8371
TTACGGG1300.039.807693
CGTAAGC301.1390362E-437.49999634
CTAGGCG301.1390362E-437.4999961
TAACGGG1150.037.1739163
ACGTTGG552.743036E-936.818182
GCGTAAG552.743036E-936.818181
ATGACGG1050.036.428572
ATGGGAC1550.036.290325
TTTAGCG250.002106017236.01
AAGTACG250.002106017236.01
TCACCTA250.002106017236.044
TACGGGA751.8189894E-1236.04
ACGGGCC250.002106017236.05
Produced by FastQC (version 0.11.5)