##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935568.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	511441
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25990681232048	33.0	31.0	34.0	30.0	34.0
2	32.284187227852286	34.0	31.0	34.0	30.0	34.0
3	32.28118590414144	34.0	31.0	34.0	30.0	34.0
4	35.91787126960881	37.0	35.0	37.0	35.0	37.0
5	35.97289227887479	37.0	35.0	37.0	35.0	37.0
6	35.912742623293795	37.0	35.0	37.0	35.0	37.0
7	36.15003294612673	37.0	35.0	37.0	35.0	37.0
8	35.99608752524729	37.0	35.0	37.0	35.0	37.0
9	37.84035108644008	39.0	38.0	39.0	35.0	39.0
10	37.49261009578817	39.0	37.0	39.0	35.0	39.0
11	37.40356756693343	39.0	37.0	39.0	35.0	39.0
12	37.2555622251638	39.0	37.0	39.0	35.0	39.0
13	37.22949079170422	39.0	37.0	39.0	34.0	39.0
14	38.40444743381934	40.0	38.0	41.0	34.0	41.0
15	38.50000293288962	40.0	38.0	41.0	34.0	41.0
16	38.30434204531901	40.0	37.0	41.0	34.0	41.0
17	38.3161537694475	40.0	37.0	41.0	34.0	41.0
18	38.23264267041555	40.0	37.0	41.0	34.0	41.0
19	38.087419663265166	40.0	37.0	41.0	34.0	41.0
20	38.20767791397248	40.0	37.0	41.0	34.0	41.0
21	38.13152445736654	40.0	36.0	41.0	34.0	41.0
22	38.21892261277449	40.0	36.0	41.0	34.0	41.0
23	38.22943213391183	40.0	36.0	41.0	34.0	41.0
24	38.18754264910322	40.0	36.0	41.0	34.0	41.0
25	37.942761726181516	40.0	36.0	41.0	34.0	41.0
26	38.00598505008398	40.0	36.0	41.0	34.0	41.0
27	38.008126059506374	40.0	36.0	41.0	34.0	41.0
28	37.88738485964168	40.0	36.0	41.0	34.0	41.0
29	37.85180695329471	40.0	36.0	41.0	34.0	41.0
30	37.693211924738144	40.0	35.0	41.0	33.0	41.0
31	37.65743262663729	40.0	35.0	41.0	33.0	41.0
32	37.58859770726242	40.0	35.0	41.0	33.0	41.0
33	37.47932215055109	40.0	35.0	41.0	33.0	41.0
34	37.3426846889475	40.0	35.0	41.0	33.0	41.0
35	37.24792693585379	40.0	35.0	41.0	33.0	41.0
36	37.13905220739049	40.0	35.0	41.0	33.0	41.0
37	37.109029193983275	40.0	35.0	41.0	33.0	41.0
38	36.97283557634214	40.0	35.0	41.0	32.0	41.0
39	36.90717404353581	39.0	35.0	41.0	32.0	41.0
40	36.810760185436834	39.0	35.0	41.0	31.0	41.0
41	36.6714283759026	39.0	35.0	41.0	31.0	41.0
42	36.66271769373203	39.0	35.0	41.0	31.0	41.0
43	36.57801975203396	39.0	35.0	41.0	31.0	41.0
44	36.46601074219705	39.0	35.0	41.0	31.0	41.0
45	36.43257775579197	39.0	35.0	41.0	31.0	41.0
46	36.32046511718849	39.0	35.0	41.0	31.0	41.0
47	36.25487397373304	38.0	35.0	41.0	31.0	41.0
48	36.15703473127888	38.0	35.0	41.0	31.0	41.0
49	36.09202429996813	38.0	35.0	40.0	31.0	41.0
50	35.96957224782526	38.0	35.0	40.0	30.0	41.0
51	34.90841563347483	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	15.0
11	12.0
12	10.0
13	21.0
14	25.0
15	25.0
16	45.0
17	84.0
18	124.0
19	258.0
20	485.0
21	785.0
22	1144.0
23	1550.0
24	2097.0
25	2972.0
26	4034.0
27	4728.0
28	5257.0
29	5685.0
30	6786.0
31	8353.0
32	11016.0
33	15385.0
34	32195.0
35	44378.0
36	35583.0
37	52478.0
38	93674.0
39	182077.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69935339560184	19.75614000441889	23.867269147369882	13.677237452609392
2	32.38965980435671	24.729538695568014	24.936796228695002	17.944005271380277
3	31.679900516384095	24.13416210276454	27.2549521841229	16.93098519672846
4	28.611706922206082	25.109250138334627	27.38673669103572	18.89230624842357
5	26.300198849916217	29.21334034619829	25.084418339554322	19.402042464331174
6	26.064785576439903	34.82024319520727	24.151563914508223	14.963407313844607
7	80.44564280141795	5.766843096271124	9.680686530802184	4.106827571508737
8	82.64706974998094	5.553524257930045	8.074831701017322	3.724574291071697
9	76.93986207597749	6.990444645618947	9.626134783875363	6.443558494528205
10	44.28174510842893	24.944812793655576	16.602306033345	14.171136064570497
11	33.42164589854939	25.999870952856728	22.844863825934954	17.73361932265892
12	30.6080271233632	22.711319585250305	26.962836377998634	19.71781691338786
13	26.12187916103715	23.097287859205657	28.908124299772602	21.872708679984594
14	21.76164992638447	26.32346644089934	29.484339347060562	22.43054428565563
15	21.47285806182922	25.232235976388285	32.81199590959661	20.482910052185883
16	25.55700462027878	24.468315993438146	29.70098212697066	20.273697259312414
17	24.35491092814225	24.89475814414566	28.284787492594454	22.465543435117638
18	25.96917337483698	24.936405176745705	27.42584188596534	21.668579562451974
19	26.186989310594967	27.159731034469274	25.358350230036308	21.29492942489945
20	28.897565897141607	25.974061524203186	26.06165716084553	19.06671541780968
21	26.6932060589589	27.44461237953156	26.271456531642944	19.590725029866594
22	25.21014154125305	24.30211891498726	28.10998727125905	22.37775227250064
23	25.135059566988176	25.95763734233274	27.729493724593844	21.17780936608524
24	25.699347529822596	23.512780555332874	28.511011045262308	22.27686086958222
25	24.269661603195676	25.871410387512928	26.316818557761305	23.542109451530088
26	22.667717292903774	26.09411447263712	27.578156620216216	23.660011614242894
27	22.80654073490393	26.66896083810254	27.50502990569782	23.01946852129571
28	21.122475515259826	28.321741901802945	28.29436826535221	22.261414317585018
29	23.842437348589574	26.253468141975322	28.271296200343738	21.63279830909137
30	23.538589983986423	25.26039171673761	29.374258223333676	21.82676007594229
31	25.216984950365735	26.16078882999212	26.39150948007688	22.230716739565267
32	27.38712774298502	26.06048400499764	26.52231635711646	20.03007189490088
33	25.888421147307316	26.456619629634698	26.302154109662695	21.35280511339529
34	23.46448563959479	27.067442774435367	28.349506590202978	21.11856499576686
35	23.8445881343107	26.75968489033926	27.46533813284426	21.93038884250578
36	23.95506030998688	30.0828443554584	25.53647439294073	20.42562094161399
37	24.998973488633098	28.638885032682165	25.5139889058562	20.84815257282854
38	23.932965874851643	28.80508211113305	24.803643039959645	22.458308974055658
39	25.609210055509823	26.406564980124784	25.042771306954272	22.941453657411117
40	25.423851431543426	25.17924843725865	27.236768268480628	22.160131862717304
41	21.99315268036782	26.520556623344625	27.302856047911682	24.18343464837586
42	23.349516366501707	26.430614675006503	26.545583948099583	23.674285010392204
43	23.451385399293372	25.470973191433615	27.23637721653133	23.841264192741683
44	23.328986139163657	25.907582692822828	26.995098163815573	23.76833300419794
45	23.59138199714141	25.2717322232672	26.76359540983222	24.37329036975917
46	22.456353714309177	27.279979508877854	26.417905486654377	23.845761290158592
47	22.646404961667134	26.03193721269902	28.194845544256324	23.12681228137752
48	22.66087388379109	25.34055736634333	28.52919496090458	23.469373788960997
49	23.05896476817463	25.310641892222176	28.477185051648185	23.153208287955014
50	20.630532163045203	25.92596213443975	29.052422469062904	24.39108323345215
51	21.047980118918897	25.207599703582623	27.969404095487064	25.775016082011415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	586.5
2	944.0
3	2898.0
4	4852.0
5	3114.5
6	1377.0
7	1342.5
8	1308.0
9	1313.0
10	1318.0
11	1247.5
12	1177.0
13	1158.5
14	1140.0
15	1179.0
16	1218.0
17	1206.0
18	1194.0
19	1237.5
20	1281.0
21	1631.5
22	1982.0
23	2040.0
24	2098.0
25	2664.5
26	3806.5
27	4382.0
28	5070.5
29	5759.0
30	6770.0
31	7781.0
32	9141.5
33	10502.0
34	12277.0
35	14052.0
36	14604.0
37	15156.0
38	16173.0
39	17190.0
40	19159.5
41	21129.0
42	23086.5
43	25044.0
44	27187.5
45	29331.0
46	33928.0
47	38525.0
48	38018.5
49	37512.0
50	37979.5
51	38447.0
52	35156.5
53	31866.0
54	30769.0
55	29672.0
56	29875.5
57	30079.0
58	29853.0
59	29627.0
60	29178.5
61	28730.0
62	26823.0
63	24916.0
64	21099.5
65	17283.0
66	13796.0
67	10309.0
68	8790.0
69	7271.0
70	6049.0
71	4827.0
72	4171.0
73	3515.0
74	2909.5
75	1864.5
76	1425.0
77	1107.5
78	790.0
79	545.5
80	301.0
81	265.5
82	230.0
83	152.0
84	74.0
85	56.0
86	38.0
87	25.0
88	12.0
89	11.0
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	511441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.12194233863575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16423489776534	20.01276510368087
2	7.329685241008008	4.122499714159534
3	2.951433240627719	2.490001062277967
4	1.9511719776618566	2.194829833942745
5	1.5106811929920032	2.1241644700691293
6	1.3586613632855227	2.292491790962863
7	1.1817329428141268	2.326283797423965
8	1.0254285459506138	2.3069633953291415
9	0.96774226426598	2.4493312938913823
>10	10.441959666527193	54.95339774506953
>50	0.08097122252260108	1.5122872641291472
>100	0.03210927789689354	1.6758043060579608
>500	0.0013960555607345014	0.2180881555617189
>1k	0.0027921111214690027	1.3210920674440758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.5482548329132784	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	1519	0.2970039554904671	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	1274	0.24910009170168212	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	1133	0.2215309292762997	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	600	0.11731558478886128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9105194929620426E-4	0.0	0.0	0.07175803269585349	0.0
2	3.9105194929620426E-4	0.0	0.0	0.3570304297074345	0.0
3	3.9105194929620426E-4	0.0	0.0	0.5324172289667821	0.0
4	3.9105194929620426E-4	0.0	0.0	0.763919982950135	0.0
5	3.9105194929620426E-4	0.0	0.0	1.4861929333002242	0.0
6	3.9105194929620426E-4	0.0	0.0	1.9804825972106264	0.0
7	3.9105194929620426E-4	0.0	0.0	2.357456676332167	0.0
8	3.9105194929620426E-4	0.0	0.0	2.8669973662651214	0.0
9	5.865779239443064E-4	0.0	0.0	3.118639295637229	0.0
10	5.865779239443064E-4	0.0	0.0	3.7703273691393533	0.0
11	5.865779239443064E-4	0.0	0.0	4.29296829937373	0.0
12	5.865779239443064E-4	0.0	0.0	4.941136905332189	0.0
13	5.865779239443064E-4	0.0	0.0	5.1720530813915975	0.0
14	5.865779239443064E-4	0.0	0.0	5.292692607749476	0.0
15	5.865779239443064E-4	0.0	0.0	5.469252562856712	0.0
16	5.865779239443064E-4	0.0	0.0	5.728520005240096	0.0
17	5.865779239443064E-4	0.0	0.0	6.022591071110842	0.0
18	5.865779239443064E-4	0.0	0.0	6.351661286443598	0.0
19	9.776298732405105E-4	0.0	0.0	6.603498741790353	0.0
20	9.776298732405105E-4	0.0	0.0	6.8150578463596	0.0
21	9.776298732405105E-4	0.0	0.0	7.0696326653514285	0.0
22	0.0011731558478886128	0.0	0.0	7.376999497498245	0.0
23	0.0011731558478886128	0.0	0.0	7.6470208684872745	0.0
24	0.0013686818225367148	0.0	0.0	7.888690973152328	0.0
25	0.0013686818225367148	0.0	0.0	8.094970876406077	0.0
26	0.0013686818225367148	0.0	0.0	8.297535786141509	0.0
27	0.0013686818225367148	0.0	0.0	8.512223306305126	0.0
28	0.0013686818225367148	0.0	0.0	8.733754235581426	0.0
29	0.0013686818225367148	0.0	0.0	8.942380450530951	0.0
30	0.001564207797184817	0.0	0.0	9.235278360553808	0.0
31	0.001564207797184817	0.0	0.0	9.470105056106178	0.0
32	0.001564207797184817	0.0	0.0	9.722529089376879	0.0
33	0.001564207797184817	0.0	0.0	9.978668116165892	0.0
34	0.001564207797184817	0.0	0.0	10.217600857185873	0.0
35	0.001564207797184817	0.0	0.0	10.475108565797424	0.0
36	0.001564207797184817	0.0	0.0	10.74317467703997	0.0
37	0.001564207797184817	0.0	0.0	11.009872106459982	0.0
38	0.001564207797184817	0.0	0.0	11.321540510049058	0.0
39	0.001564207797184817	0.0	0.0	11.64376731626913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTG	20	7.029611E-4	45.0	37
CGGTCTA	40	6.8030204E-9	45.0	31
TAGGACG	20	7.029611E-4	45.0	1
TATCGAG	35	1.2101555E-7	45.0	1
GCGATAT	30	2.1631295E-6	44.999996	9
CGTAAGG	135	0.0	43.333332	2
CGTTTTT	1135	0.0	40.837	1
TTACGGG	130	0.0	39.80769	3
CGTAAGC	30	1.1390362E-4	37.499996	34
CTAGGCG	30	1.1390362E-4	37.499996	1
TAACGGG	115	0.0	37.173916	3
ACGTTGG	55	2.743036E-9	36.81818	2
GCGTAAG	55	2.743036E-9	36.81818	1
ATGACGG	105	0.0	36.42857	2
ATGGGAC	155	0.0	36.29032	5
TTTAGCG	25	0.0021060172	36.0	1
AAGTACG	25	0.0021060172	36.0	1
TCACCTA	25	0.0021060172	36.0	44
TACGGGA	75	1.8189894E-12	36.0	4
ACGGGCC	25	0.0021060172	36.0	5
>>END_MODULE