##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935567.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260640
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90608885819521	33.0	31.0	34.0	30.0	34.0
2	31.8722605893186	33.0	31.0	34.0	30.0	34.0
3	31.847847605893186	33.0	31.0	34.0	30.0	34.0
4	35.56525475751995	37.0	35.0	37.0	33.0	37.0
5	35.65920042971148	37.0	35.0	37.0	33.0	37.0
6	35.6220265500307	37.0	35.0	37.0	33.0	37.0
7	35.866194751381215	37.0	35.0	37.0	35.0	37.0
8	35.72598219766728	37.0	35.0	37.0	35.0	37.0
9	37.45622314303254	39.0	37.0	39.0	35.0	39.0
10	37.043335635359114	39.0	37.0	39.0	33.0	39.0
11	37.09508517495396	39.0	37.0	39.0	34.0	39.0
12	36.86033993247391	39.0	35.0	39.0	33.0	39.0
13	36.7137277470841	39.0	35.0	39.0	33.0	39.0
14	37.69187001227747	40.0	36.0	41.0	33.0	41.0
15	37.90956108041743	40.0	36.0	41.0	33.0	41.0
16	37.71549263351749	39.0	36.0	41.0	33.0	41.0
17	37.761602209944755	39.0	36.0	41.0	33.0	41.0
18	37.708471454880296	39.0	36.0	41.0	33.0	41.0
19	37.621815531000614	39.0	36.0	41.0	33.0	41.0
20	37.72618937998772	39.0	35.0	41.0	33.0	41.0
21	37.62998772252916	39.0	35.0	41.0	33.0	41.0
22	37.6970879373849	39.0	35.0	41.0	33.0	41.0
23	37.778974831184776	39.0	35.0	41.0	34.0	41.0
24	37.761310620012274	39.0	35.0	41.0	34.0	41.0
25	37.41827424800491	39.0	35.0	41.0	33.0	41.0
26	37.595250153468385	39.0	35.0	41.0	33.0	41.0
27	37.56173265807244	39.0	35.0	41.0	33.0	41.0
28	37.20038367096378	39.0	35.0	41.0	32.0	41.0
29	36.967744782074895	39.0	35.0	41.0	31.0	41.0
30	36.8451197053407	39.0	35.0	41.0	31.0	41.0
31	36.861088090853286	39.0	35.0	41.0	31.0	41.0
32	36.98012968078576	39.0	35.0	41.0	32.0	41.0
33	36.917065684469	39.0	35.0	41.0	31.0	41.0
34	36.847410220994476	39.0	35.0	41.0	31.0	41.0
35	36.83582719459791	39.0	35.0	41.0	31.0	41.0
36	36.699221147943526	39.0	35.0	41.0	31.0	41.0
37	36.67719076120319	39.0	35.0	41.0	31.0	41.0
38	36.46175567833026	39.0	35.0	41.0	30.0	41.0
39	36.37996470227133	39.0	35.0	41.0	30.0	41.0
40	36.34033532842235	39.0	35.0	41.0	30.0	41.0
41	36.12504220380602	39.0	35.0	41.0	30.0	41.0
42	36.14385742786986	39.0	35.0	41.0	30.0	41.0
43	35.953867403314916	39.0	35.0	41.0	29.0	41.0
44	35.91499002455494	38.0	35.0	40.0	29.0	41.0
45	35.95227516881523	38.0	35.0	40.0	29.0	41.0
46	35.7068792203806	38.0	35.0	40.0	28.0	41.0
47	35.73063612645795	38.0	35.0	40.0	29.0	41.0
48	35.627628146101905	38.0	35.0	40.0	28.0	41.0
49	35.39699585635359	38.0	35.0	40.0	27.0	41.0
50	35.16217004297115	38.0	34.0	40.0	26.0	41.0
51	33.900184162062615	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	2.0
11	7.0
12	2.0
13	4.0
14	5.0
15	15.0
16	20.0
17	30.0
18	71.0
19	139.0
20	285.0
21	520.0
22	776.0
23	1163.0
24	1608.0
25	2147.0
26	2608.0
27	3006.0
28	3426.0
29	3902.0
30	4774.0
31	5661.0
32	7324.0
33	9971.0
34	18881.0
35	26276.0
36	17519.0
37	26347.0
38	47444.0
39	76662.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8994014732965	20.368707796193984	23.70817986494782	13.023710865561695
2	31.279542664211174	25.016114180478823	25.786909146715775	17.91743400859423
3	30.474984653161446	24.61671270718232	27.954266421117254	16.95403621853898
4	28.265423572744012	25.659914057704114	27.458947206875383	18.615715162676487
5	24.244935543278086	29.125997544505832	26.350521792510744	20.278545119705342
6	24.218845917740943	35.021869244935544	25.836786372007364	14.922498465316144
7	78.29765193370166	5.9369244935543275	10.504910988336402	5.2605125844076115
8	78.21516267648865	6.341697360343769	9.54573357888275	5.897406384284838
9	72.80885512584408	8.110036832412524	11.95633824432167	7.124769797421732
10	40.45234806629834	26.423802946593	18.958717004297114	14.165131982811541
11	32.00851749539595	27.27171577655003	22.95158072437078	17.768186003683244
12	28.32105586249233	21.58034069981584	31.79135972989564	18.307243707796196
13	26.41306015960712	26.920656844689994	30.24938612645795	16.416896869244933
14	18.535911602209946	32.24946286065071	30.556322897483117	18.65830263965623
15	17.364564149785146	24.24033149171271	40.29274094536526	18.102363413136892
16	20.15231737262124	25.865945365254756	32.38873542050338	21.593001841620627
17	20.021101903007978	23.11732658072437	27.394490484960098	29.46708103130755
18	25.54059238796808	22.94429097605893	29.85804174340086	21.65707489257213
19	27.010052179251076	24.055785758133823	26.634438305709025	22.29972375690608
20	25.017648864333946	24.964318600368323	27.253683241252304	22.764349294045427
21	24.656998158379373	27.055709023941066	28.853974831184775	19.43331798649478
22	19.94398403928791	27.3116175567833	26.903007980356048	25.841390423572747
23	18.743477593615715	26.32673419275629	28.65024554941682	26.279542664211174
24	21.592618170656845	23.05900859422959	30.083640270104357	25.264732965009205
25	19.142879066912215	26.286065070595455	28.07013505217925	26.500920810313076
26	17.252148557397177	26.695058317986497	26.868477593615715	29.184315531000614
27	20.475751995089013	25.791896869244933	27.551028238182933	26.181322897483117
28	19.592157765500307	25.804174340085943	31.23695518723143	23.366712707182323
29	19.332796193984038	23.877378759975446	32.61318293431553	24.176642111724984
30	21.994321669736035	26.482504604051567	28.28000306936771	23.24317065684469
31	21.64134438305709	31.435696746470228	24.812384898710864	22.11057397176182
32	23.078959484346225	28.758824432166975	25.81645181092695	22.345764272559855
33	21.639042357274402	25.307704112952734	28.85934622467772	24.19390730509515
34	21.052025782688766	27.308931860036832	27.983425414364643	23.65561694290976
35	20.033379373848987	25.906998158379373	28.016037446286063	26.043585021485573
36	21.873465316144873	25.672958870472684	28.453806015960716	23.99976979742173
37	19.38267341927563	29.688842848373238	28.236264579496623	22.692219152854513
38	20.778468385512586	27.64732965009208	26.035144260282383	25.539057704112956
39	22.324662369551874	24.72874462860651	28.898864333947206	24.047728667894415
40	25.639579496623693	23.167971147943522	27.816144874155924	23.376304481276858
41	19.582949662369554	24.04696132596685	25.634208103130756	30.73588090853284
42	22.559085328422345	23.36594536525476	26.984729895641497	27.0902394106814
43	21.419198895027623	24.56645181092695	27.021945979128297	26.99240331491713
44	19.06767955801105	24.544582565991405	28.443830570902396	27.943907305095152
45	18.85512584407612	25.166513198281155	27.622007366482503	28.35635359116022
46	22.140883977900554	26.88612645794966	27.12438612645795	23.848603437691835
47	19.03314917127072	24.401856967464703	28.990561694290978	27.574432166973605
48	19.811233885819522	23.202117863720073	29.420273173726212	27.566375076734197
49	22.264426028238184	21.3286525475752	31.57612031921424	24.830801104972373
50	20.028391651319826	22.423649478207487	31.54120626151013	26.006752608962554
51	20.115868631062	23.097759361571516	27.320058317986494	29.466313689379987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	321.0
2	411.0
3	717.5
4	1024.0
5	746.0
6	468.0
7	465.5
8	463.0
9	461.0
10	459.0
11	455.0
12	451.0
13	523.0
14	595.0
15	576.5
16	558.0
17	525.5
18	493.0
19	535.0
20	577.0
21	626.0
22	675.0
23	852.5
24	1030.0
25	981.0
26	1320.5
27	1709.0
28	1907.5
29	2106.0
30	2587.0
31	3068.0
32	3589.0
33	4110.0
34	4603.0
35	5096.0
36	5848.0
37	6600.0
38	7560.0
39	8520.0
40	9733.5
41	10947.0
42	13730.5
43	16514.0
44	18340.0
45	20166.0
46	24520.5
47	28875.0
48	29225.5
49	29576.0
50	28741.5
51	27907.0
52	23780.5
53	19654.0
54	16804.0
55	13954.0
56	13546.5
57	13139.0
58	12458.5
59	11778.0
60	10865.5
61	9953.0
62	8642.0
63	7331.0
64	5927.5
65	4524.0
66	3524.5
67	2525.0
68	2001.0
69	1477.0
70	1242.0
71	1007.0
72	925.5
73	844.0
74	616.0
75	336.5
76	285.0
77	207.5
78	130.0
79	83.0
80	36.0
81	25.0
82	14.0
83	8.0
84	2.0
85	18.0
86	34.0
87	18.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.538367096378146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36345053147997	28.66559238796808
2	10.375102207686018	7.789287906691221
3	3.590556009811938	4.043508287292818
4	1.6741618969746526	2.5138121546961325
5	0.9280457890433361	1.741866175567833
6	0.6674161896974652	1.5032228360957642
7	0.46913327882256745	1.2327348066298343
8	0.4118969746524938	1.2369551872314304
9	0.34035159443990187	1.1498618784530388
>10	4.976492232215863	41.92142418661756
>50	0.16557645134914145	3.9226519337016574
>100	0.02964022894521668	1.8707796193984039
>500	0.006132461161079314	1.4226519337016574
>1k	0.002044153720359771	0.9856507059545734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1524	0.5847145488029466	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1045	0.4009361571516268	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	771	0.2958103130755064	Illumina PCR Primer Index 10 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	655	0.25130448127685695	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	640	0.24554941682013504	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	586	0.22483118477593617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	547	0.2098680171884592	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	509	0.19528852056476367	Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	391	0.15001534683855125	No Hit
ACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	344	0.1319828115408226	Illumina PCR Primer Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.83670963781461E-4	0.0	0.0	0.3161448741559239	0.0
2	3.83670963781461E-4	0.0	0.0	1.732658072437078	0.0
3	3.83670963781461E-4	0.0	0.0	2.3687845303867405	0.0
4	3.83670963781461E-4	0.0	0.0	3.145718232044199	0.0
5	3.83670963781461E-4	0.0	0.0	5.082872928176796	0.0
6	3.83670963781461E-4	0.0	0.0	6.130294659300184	0.0
7	3.83670963781461E-4	0.0	0.0	7.152394106813996	0.0
8	3.83670963781461E-4	0.0	0.0	8.670963781461019	0.0
9	3.83670963781461E-4	0.0	0.0	9.168968692449356	0.0
10	3.83670963781461E-4	0.0	0.0	10.58548189073051	0.0
11	3.83670963781461E-4	0.0	0.0	12.305095150399017	0.0
12	3.83670963781461E-4	0.0	0.0	14.01818600368324	0.0
13	3.83670963781461E-4	0.0	0.0	14.859576427255986	0.0
14	3.83670963781461E-4	0.0	0.0	15.333026396562309	0.0
15	3.83670963781461E-4	0.0	0.0	15.79304788213628	0.0
16	3.83670963781461E-4	0.0	0.0	16.73073971761817	3.83670963781461E-4
17	7.67341927562922E-4	0.0	0.0	17.824201964395336	3.83670963781461E-4
18	7.67341927562922E-4	0.0	0.0	19.292510742786988	3.83670963781461E-4
19	7.67341927562922E-4	0.0	0.0	20.008824432166975	3.83670963781461E-4
20	7.67341927562922E-4	0.0	0.0	20.737031921424187	3.83670963781461E-4
21	7.67341927562922E-4	0.0	0.0	21.548496009821978	3.83670963781461E-4
22	7.67341927562922E-4	0.0	0.0	22.33732351135666	3.83670963781461E-4
23	7.67341927562922E-4	0.0	0.0	23.114640883977902	3.83670963781461E-4
24	0.0011510128913443832	0.0	0.0	23.71048189073051	3.83670963781461E-4
25	0.0011510128913443832	0.0	0.0	24.24148250460405	3.83670963781461E-4
26	0.0011510128913443832	0.0	0.0	24.773250460405155	3.83670963781461E-4
27	0.0011510128913443832	0.0	0.0	25.347989564149785	3.83670963781461E-4
28	0.0011510128913443832	0.0	0.0	25.860573971761816	3.83670963781461E-4
29	0.0011510128913443832	0.0	0.0	26.429941682013506	3.83670963781461E-4
30	0.0011510128913443832	0.0	0.0	27.08717004297115	3.83670963781461E-4
31	0.001534683855125844	0.0	0.0	27.66306015960712	3.83670963781461E-4
32	0.001534683855125844	0.0	0.0	28.171424186617557	3.83670963781461E-4
33	0.001534683855125844	0.0	0.0	28.764963167587478	3.83670963781461E-4
34	0.001534683855125844	0.0	0.0	29.409914057704114	3.83670963781461E-4
35	0.001534683855125844	0.0	0.0	30.004604051565376	3.83670963781461E-4
36	0.001534683855125844	0.0	0.0	30.507596685082873	3.83670963781461E-4
37	0.0019183548189073052	0.0	0.0	31.038597298956415	3.83670963781461E-4
38	0.0019183548189073052	0.0	0.0	31.6018262737876	3.83670963781461E-4
39	0.0019183548189073052	0.0	0.0	32.338858195211785	3.83670963781461E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	25	3.8824783E-5	45.0	28
AGCCGTA	20	7.02324E-4	45.0	44
CAGCGAT	20	7.02324E-4	45.0	21
ATCTCTC	20	7.02324E-4	45.0	26
CTCACTC	20	7.02324E-4	45.0	28
GGTGTCG	20	7.02324E-4	45.0	31
TGGACGG	35	1.2070996E-7	45.0	2
GCGCGAG	25	3.8824783E-5	45.0	1
TATATCC	25	3.8824783E-5	45.0	15
GTCTACC	20	7.02324E-4	45.0	23
CGCGAGG	35	1.2070996E-7	45.0	2
TCGATGA	20	7.02324E-4	45.0	37
ACGGGCC	20	7.02324E-4	45.0	5
ATGTATG	25	3.8824783E-5	45.0	1
AACGGGC	45	3.8198777E-10	45.0	4
TACGCGG	25	3.8824783E-5	45.0	2
TAACCCA	20	7.02324E-4	45.0	21
AGGCCCA	20	7.02324E-4	45.0	44
CTACGAA	25	3.8824783E-5	45.0	28
TACGAAC	20	7.02324E-4	45.0	29
>>END_MODULE