FastQCFastQC Report
Sat 14 Jan 2017
SRR2935566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669838
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.5577467984796324No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC17660.26364583675455855No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC13860.20691570200555956No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG13740.20512422406611747No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC13110.1957189648840466No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC12580.1878066039848441No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT8140.12152192022548734Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC7430.11092234241712176No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG7270.10853370516453233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC207.031177E-445.09
GAGTACG207.031177E-445.01
ATTACGG253.889056E-545.02
ACGTCGC207.031177E-445.020
ATCGAGC207.031177E-445.037
GTACGCT207.031177E-445.010
ATAGACG302.1641408E-644.9999961
CTAACGG556.002665E-1140.909092
GCGATAC556.002665E-1140.909099
CGCACGG556.002665E-1140.909092
CGTTTTT20850.040.7913631
AACACGT1950.040.38461741
CACGACC2000.039.37527
ACGATAG356.244387E-638.5714261
TACGCGG356.244387E-638.5714262
CGAGACA2050.038.4146322
CGGGCAC900.037.5000046
GCGAGAC2100.037.521
GCTGCGA601.546141E-1037.49999628
TCGATAG301.13941336E-437.4999961