##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669838
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16878558696282	33.0	31.0	34.0	30.0	34.0
2	32.17425556627125	33.0	31.0	34.0	30.0	34.0
3	32.1612927304811	33.0	31.0	34.0	30.0	34.0
4	35.82368124830183	37.0	35.0	37.0	35.0	37.0
5	35.90460827841956	37.0	35.0	37.0	35.0	37.0
6	35.86104401362717	37.0	35.0	37.0	35.0	37.0
7	36.122775954783094	37.0	35.0	37.0	35.0	37.0
8	35.96678450610446	37.0	35.0	37.0	35.0	37.0
9	37.78656630409144	39.0	38.0	39.0	35.0	39.0
10	37.37746440184044	39.0	37.0	39.0	34.0	39.0
11	37.37511308704493	39.0	37.0	39.0	34.0	39.0
12	37.39433116664029	39.0	37.0	39.0	35.0	39.0
13	37.41152039746924	39.0	37.0	39.0	35.0	39.0
14	38.61420223994458	40.0	38.0	41.0	35.0	41.0
15	38.713627772685335	40.0	38.0	41.0	35.0	41.0
16	38.472771028218766	40.0	38.0	41.0	34.0	41.0
17	38.51494839050636	40.0	38.0	41.0	34.0	41.0
18	38.412571397860376	40.0	38.0	41.0	34.0	41.0
19	38.27765220844443	40.0	37.0	41.0	34.0	41.0
20	38.416130467366735	40.0	37.0	41.0	34.0	41.0
21	38.33727558006563	40.0	37.0	41.0	34.0	41.0
22	38.4313923665125	40.0	37.0	41.0	34.0	41.0
23	38.44951018007339	40.0	37.0	41.0	34.0	41.0
24	38.39818135131181	40.0	37.0	41.0	34.0	41.0
25	38.13714808655227	40.0	37.0	41.0	34.0	41.0
26	38.252086325350305	40.0	37.0	41.0	34.0	41.0
27	38.263220360743944	40.0	37.0	41.0	34.0	41.0
28	38.098846288207	40.0	37.0	41.0	34.0	41.0
29	38.00553865262944	40.0	37.0	41.0	34.0	41.0
30	37.87490109548876	40.0	37.0	41.0	33.0	41.0
31	37.86558093150881	40.0	37.0	41.0	33.0	41.0
32	37.82568023910259	40.0	37.0	41.0	33.0	41.0
33	37.73507624231531	40.0	37.0	41.0	33.0	41.0
34	37.62203099853995	40.0	37.0	41.0	33.0	41.0
35	37.53244665127986	40.0	37.0	41.0	33.0	41.0
36	37.412974480396755	40.0	36.0	41.0	33.0	41.0
37	37.40494268763492	40.0	36.0	41.0	33.0	41.0
38	37.283552142458326	40.0	36.0	41.0	33.0	41.0
39	37.216147784986816	40.0	36.0	41.0	32.0	41.0
40	37.15234429817359	40.0	36.0	41.0	32.0	41.0
41	36.99990594740818	40.0	35.0	41.0	31.0	41.0
42	36.987994112009176	40.0	35.0	41.0	31.0	41.0
43	36.916237956043105	40.0	35.0	41.0	31.0	41.0
44	36.82016696574396	40.0	35.0	41.0	31.0	41.0
45	36.812989110799926	39.0	35.0	41.0	31.0	41.0
46	36.678111722535895	39.0	35.0	41.0	31.0	41.0
47	36.64187310961755	39.0	35.0	41.0	31.0	41.0
48	36.54609920607669	39.0	35.0	41.0	31.0	41.0
49	36.451144903693134	39.0	35.0	41.0	31.0	41.0
50	36.33222062647984	39.0	35.0	41.0	30.0	41.0
51	35.23793962122185	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	18.0
11	14.0
12	15.0
13	12.0
14	24.0
15	29.0
16	47.0
17	80.0
18	146.0
19	259.0
20	480.0
21	863.0
22	1344.0
23	1953.0
24	2925.0
25	4048.0
26	5793.0
27	6609.0
28	6860.0
29	7398.0
30	8504.0
31	10503.0
32	13491.0
33	18323.0
34	32753.0
35	45847.0
36	43897.0
37	66333.0
38	129074.0
39	261999.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77323472242542	20.34148555322332	23.97191559750268	13.913364126848581
2	31.996094578092016	25.41420462858184	25.199973724990222	17.389727068335926
3	30.8641791000212	24.044470454050085	28.53062979406961	16.560720651859107
4	27.422451398696403	25.656054150406515	28.325505570003497	18.59598888089359
5	24.8406331083038	29.88528569594439	26.428330432134338	18.84575076361747
6	25.338066816155546	34.21006273158584	25.99837572666824	14.453494725590366
7	80.98390954230725	5.42175869389315	9.64994521063302	3.9443865531665865
8	82.5180118177828	5.198570400604325	8.382773148134325	3.9006446334785427
9	75.80668758714793	7.953117022324801	10.441927749694702	5.7982676408325595
10	35.509779976651075	33.97224403512491	17.655164383029927	12.862811605194091
11	25.869687894684983	26.64450210349368	29.783619322881055	17.70219067894028
12	23.83994338929711	23.637954251625022	32.65267124289753	19.86943111618033
13	23.477169106560094	25.20997614348544	33.442563724363204	17.87029102559126
14	19.73014370638871	29.545352756935262	31.047805588814008	19.67669794786202
15	19.019822703399928	26.985330781472534	35.05623747831565	18.938609036811886
16	22.10459842529089	27.73625861775534	30.737730615462244	19.421412341491525
17	22.44184414739086	25.659786396113688	29.852740513377864	22.045628943117592
18	24.48621905595084	24.858398597869932	30.622329578196517	20.033052767982706
19	24.061937363959643	26.775578572729525	28.65200242446681	20.51048163884402
20	24.369175830573962	26.898891970894457	29.008058664930925	19.72387353360066
21	24.057309379282753	27.81314287932306	29.622834177816127	18.50671356357806
22	22.419599962976122	25.52796347773641	29.841394486428065	22.211042072859406
23	21.000749434937998	26.729448015788893	30.393169691776222	21.876632857496887
24	20.58184217676513	26.107805170802497	31.398487395459796	21.911865256972582
25	20.916549971784224	28.24175397633458	28.843690563987114	21.998005487894087
26	21.131378034688986	28.25533935070868	28.737844075731743	21.875438538870593
27	21.464592931425212	27.5529307086191	29.049411947366377	21.933064412589314
28	20.42628217569024	27.6311585786414	30.41108447117064	21.531474774497713
29	20.73396851178942	27.1150636422538	30.628749040812853	21.52221880514393
30	22.45931105730042	26.66823918619129	29.42726450276037	21.44518525374792
31	22.82835551282549	27.35168802008844	27.545018347719896	22.274938119366176
32	22.271056583830717	28.154867296271636	28.864591139947272	20.709484979950375
33	22.245677313021954	25.88775196390769	28.991039624506225	22.87553109856413
34	21.257527939591363	27.061020724413964	30.080855371000155	21.600595964994522
35	21.846327022354657	26.953084178562577	29.842439515226072	21.358149283856694
36	21.955607176660624	27.840761497556127	29.01671747497156	21.18691385081169
37	21.172134157811293	27.62130544997447	30.34405333826985	20.862507053944388
38	21.231999378954313	27.690128060814708	28.2448890627286	22.832983497502383
39	22.26941439571956	25.60544489861728	30.233728155165874	21.891412550497286
40	23.540766573410288	25.774739563894556	29.660455214544413	21.024038648150746
41	21.086143216718074	26.291580949423594	29.282751948978706	23.33952388487963
42	22.122363914857026	26.393545902143504	29.626715713351587	21.857374469647887
43	22.219253013415184	25.606788507071858	29.630597248887042	22.543361230625912
44	21.051060107070665	26.403399030810437	28.72336296238792	23.822177899730978
45	21.60432821070169	25.47556274800773	28.518537317978378	24.401571723312205
46	22.212982840627138	26.464757150236323	28.455686300269615	22.86657370886692
47	20.95252882040135	26.06406325111445	30.224322895983807	22.759085032500394
48	21.6110462529746	25.279843783123678	29.875133987620888	23.23397597628083
49	22.066230939421175	24.613264699822942	30.824468005696897	22.496036355058983
50	20.48958106288386	25.371358447863514	30.707126200663442	23.431934288589183
51	21.218563294408497	24.492190649082314	29.060608684487892	25.228637372021296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	553.5
2	915.0
3	3457.0
4	5999.0
5	3930.0
6	1861.0
7	1885.0
8	1909.0
9	1893.0
10	1877.0
11	1945.5
12	2014.0
13	2003.5
14	1993.0
15	1997.5
16	2002.0
17	2041.0
18	2080.0
19	2142.5
20	2205.0
21	2648.5
22	3092.0
23	3339.0
24	3586.0
25	4364.0
26	6235.5
27	7329.0
28	8331.0
29	9333.0
30	11152.0
31	12971.0
32	14645.5
33	16320.0
34	18680.0
35	21040.0
36	23277.0
37	25514.0
38	27356.0
39	29198.0
40	32444.5
41	35691.0
42	38767.5
43	41844.0
44	45030.0
45	48216.0
46	55101.5
47	61987.0
48	62852.5
49	63718.0
50	62231.0
51	60744.0
52	53513.5
53	46283.0
54	41209.0
55	36135.0
56	32557.0
57	28979.0
58	26002.5
59	23026.0
60	21476.0
61	19926.0
62	18145.5
63	16365.0
64	13624.0
65	10883.0
66	8780.5
67	6678.0
68	5730.5
69	4783.0
70	3747.5
71	2712.0
72	2328.5
73	1945.0
74	1718.0
75	1273.5
76	1056.0
77	753.5
78	451.0
79	315.0
80	179.0
81	135.0
82	91.0
83	50.5
84	10.0
85	30.0
86	50.0
87	29.5
88	9.0
89	5.5
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.29591636831704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7723733989517	23.087740281858487
2	8.973403620922879	5.616617785191115
3	3.2664979683291997	3.066841417023245
4	1.7764923141513942	2.223878195705601
5	1.2356559077771698	1.9335491974905594
6	0.9925105041125749	1.8636915438829997
7	0.8041271694049559	1.7616127650232305
8	0.6952696890000305	1.74072816322966
9	0.6102723298304055	1.7189128616643328
>10	7.686608996258309	49.99031826752037
>50	0.14176737941980347	2.7485304830384196
>100	0.03927339564905303	2.038502185117466
>500	0.002873663096272173	0.5856197086216132
>1k	0.002873663096272173	1.623457144632888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3736	0.5577467984796324	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1766	0.26364583675455855	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1386	0.20691570200555956	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	1374	0.20512422406611747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	1311	0.1957189648840466	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1258	0.1878066039848441	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	814	0.12152192022548734	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	743	0.11092234241712176	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	727	0.10853370516453233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.98579656573679E-4	0.0	0.0	0.1254034557609452	0.0
2	2.98579656573679E-4	0.0	0.0	0.6562780851489465	0.0
3	2.98579656573679E-4	0.0	0.0	0.9977039224409484	0.0
4	2.98579656573679E-4	0.0	0.0	1.4212391652907121	0.0
5	2.98579656573679E-4	0.0	0.0	2.5267303437547586	0.0
6	2.98579656573679E-4	0.0	0.0	3.305724667755487	0.0
7	2.98579656573679E-4	0.0	0.0	3.922590238236708	0.0
8	2.98579656573679E-4	0.0	0.0	4.875357922363317	0.0
9	2.98579656573679E-4	0.0	0.0	5.270378808010295	0.0
10	2.98579656573679E-4	0.0	0.0	6.2276251869855095	0.0
11	2.98579656573679E-4	0.0	0.0	7.190096709950764	0.0
12	2.98579656573679E-4	0.0	0.0	8.115842935157456	0.0
13	4.4786948486051853E-4	0.0	0.0	8.512804588572163	0.0
14	4.4786948486051853E-4	0.0	0.0	8.690011614748641	0.0
15	4.4786948486051853E-4	0.0	0.0	8.948730887169734	0.0
16	4.4786948486051853E-4	0.0	0.0	9.433922829101961	0.0
17	4.4786948486051853E-4	0.0	0.0	10.053027747007485	0.0
18	4.4786948486051853E-4	0.0	0.0	10.799775468098257	0.0
19	4.4786948486051853E-4	0.0	0.0	11.235552476867541	0.0
20	5.97159313147358E-4	0.0	0.0	11.659684879030452	0.0
21	5.97159313147358E-4	0.0	0.0	12.151893442892161	0.0
22	5.97159313147358E-4	0.0	0.0	12.684111680734745	0.0
23	5.97159313147358E-4	0.0	0.0	13.204685311970954	0.0
24	5.97159313147358E-4	0.0	0.0	13.615530919416337	0.0
25	5.97159313147358E-4	0.0	0.0	13.970691420910727	0.0
26	5.97159313147358E-4	0.0	0.0	14.332719254506314	0.0
27	5.97159313147358E-4	0.0	0.0	14.742221253497114	0.0
28	7.464491414341975E-4	0.0	0.0	15.109623520911025	0.0
29	7.464491414341975E-4	0.0	0.0	15.507928782780313	0.0
30	7.464491414341975E-4	0.0	0.0	16.003421722864335	0.0
31	0.0010450287980078765	0.0	0.0	16.413968750653144	0.0
32	0.0010450287980078765	0.0	0.0	16.839444761270634	0.0
33	0.0010450287980078765	0.0	0.0	17.27611750900964	0.0
34	0.0010450287980078765	0.0	0.0	17.715626763486096	0.0
35	0.0010450287980078765	0.0	0.0	18.153643119679685	0.0
36	0.0010450287980078765	0.0	0.0	18.54776826635694	0.0
37	0.0010450287980078765	0.0	0.0	18.962047539852918	0.0
38	0.001194318626294716	0.0	0.0	19.377073262490335	0.0
39	0.001194318626294716	0.0	0.0	19.820911921987108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	20	7.031177E-4	45.0	9
GAGTACG	20	7.031177E-4	45.0	1
ATTACGG	25	3.889056E-5	45.0	2
ACGTCGC	20	7.031177E-4	45.0	20
ATCGAGC	20	7.031177E-4	45.0	37
GTACGCT	20	7.031177E-4	45.0	10
ATAGACG	30	2.1641408E-6	44.999996	1
CTAACGG	55	6.002665E-11	40.90909	2
GCGATAC	55	6.002665E-11	40.90909	9
CGCACGG	55	6.002665E-11	40.90909	2
CGTTTTT	2085	0.0	40.791363	1
AACACGT	195	0.0	40.384617	41
CACGACC	200	0.0	39.375	27
ACGATAG	35	6.244387E-6	38.571426	1
TACGCGG	35	6.244387E-6	38.571426	2
CGAGACA	205	0.0	38.41463	22
CGGGCAC	90	0.0	37.500004	6
GCGAGAC	210	0.0	37.5	21
GCTGCGA	60	1.546141E-10	37.499996	28
TCGATAG	30	1.13941336E-4	37.499996	1
>>END_MODULE