##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558066
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.054638698648546	33.0	31.0	34.0	30.0	34.0
2	32.03795070833916	33.0	31.0	34.0	30.0	34.0
3	32.05297581289668	33.0	31.0	34.0	30.0	34.0
4	35.72019617751305	37.0	35.0	37.0	33.0	37.0
5	35.8085961158716	37.0	35.0	37.0	35.0	37.0
6	35.752101005974204	37.0	35.0	37.0	35.0	37.0
7	36.021140868642775	37.0	35.0	37.0	35.0	37.0
8	35.865791859744185	37.0	35.0	37.0	35.0	37.0
9	37.66735475732261	39.0	38.0	39.0	35.0	39.0
10	37.276874061490936	39.0	37.0	39.0	34.0	39.0
11	37.251672741216986	39.0	37.0	39.0	34.0	39.0
12	37.00926772102225	39.0	35.0	39.0	33.0	39.0
13	36.84688011812223	39.0	35.0	39.0	33.0	39.0
14	37.62858514942677	40.0	36.0	41.0	33.0	41.0
15	37.866325846763644	40.0	36.0	41.0	33.0	41.0
16	37.748631882250486	40.0	36.0	41.0	33.0	41.0
17	37.848037687298635	40.0	36.0	41.0	33.0	41.0
18	37.8043260116187	39.0	36.0	41.0	33.0	41.0
19	37.69654664502048	39.0	36.0	41.0	33.0	41.0
20	37.81394673748267	40.0	36.0	41.0	33.0	41.0
21	37.72235542032663	40.0	35.0	41.0	33.0	41.0
22	37.871117394716755	40.0	35.0	41.0	34.0	41.0
23	37.93998738500464	40.0	35.0	41.0	34.0	41.0
24	37.88670694864048	40.0	35.0	41.0	34.0	41.0
25	37.57146287356692	39.0	35.0	41.0	33.0	41.0
26	37.698426351005075	40.0	35.0	41.0	33.0	41.0
27	37.69764328950339	40.0	35.0	41.0	34.0	41.0
28	37.49501313464716	40.0	35.0	41.0	33.0	41.0
29	37.39486906566607	40.0	35.0	41.0	33.0	41.0
30	37.2447721237273	39.0	35.0	41.0	33.0	41.0
31	37.19853207326732	39.0	35.0	41.0	32.0	41.0
32	37.22043987628703	39.0	35.0	41.0	33.0	41.0
33	37.17472664523551	39.0	35.0	41.0	33.0	41.0
34	37.065864969376385	39.0	35.0	41.0	32.0	41.0
35	36.96585350119878	39.0	35.0	41.0	32.0	41.0
36	36.83826106589543	39.0	35.0	41.0	31.0	41.0
37	36.80009353732354	39.0	35.0	41.0	31.0	41.0
38	36.6527758365498	39.0	35.0	41.0	31.0	41.0
39	36.58760433353761	39.0	35.0	41.0	31.0	41.0
40	36.47225417782126	39.0	35.0	41.0	31.0	41.0
41	36.24371848491039	39.0	35.0	41.0	30.0	41.0
42	36.295741005544144	39.0	35.0	41.0	30.0	41.0
43	36.20175570631431	39.0	35.0	41.0	30.0	41.0
44	36.10257388910989	38.0	35.0	41.0	30.0	41.0
45	36.12685596327316	38.0	35.0	41.0	30.0	41.0
46	36.00785390975261	38.0	35.0	40.0	30.0	41.0
47	35.96029143506323	38.0	35.0	40.0	30.0	41.0
48	35.843943189515215	38.0	35.0	40.0	30.0	41.0
49	35.750882512104305	38.0	35.0	40.0	29.0	41.0
50	35.595072984199	37.0	35.0	40.0	29.0	41.0
51	34.41741299416198	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	19.0
10	14.0
11	16.0
12	7.0
13	14.0
14	21.0
15	37.0
16	55.0
17	93.0
18	164.0
19	338.0
20	585.0
21	990.0
22	1369.0
23	2054.0
24	2889.0
25	3835.0
26	4726.0
27	5459.0
28	6253.0
29	7313.0
30	9024.0
31	11468.0
32	15156.0
33	22549.0
34	42847.0
35	47882.0
36	39017.0
37	58840.0
38	102481.0
39	172418.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.786935595431366	20.22000981962707	23.849150458906294	12.143904126035272
2	32.16770059455333	24.002178953743822	25.40774747072927	18.422372980973574
3	31.57959811205126	23.80381531933499	26.977991850426292	17.638594718187456
4	28.314034540717408	25.443943906276317	26.282912773757943	19.959108779248332
5	25.17193306884849	28.814154598201647	25.875613278716138	20.13829905423373
6	25.510602688570888	33.7374074034254	25.494833944372168	15.257155963631542
7	79.48450541692202	6.232416954267058	9.345310411313356	4.937767217497572
8	80.48062415556583	5.50956338497597	8.865438854902468	5.144373604555733
9	75.07642465228128	6.992542100755108	11.230571294434709	6.7004619525289115
10	43.04275838341701	23.328781900348705	18.586332082585212	15.042127633649066
11	33.694939308253865	24.606229370719593	23.457619708063206	18.24121161296334
12	30.601756781455958	20.62713012439389	29.897718191038336	18.873394903111816
13	28.464733561980125	23.211591460508256	30.139266681718652	18.18440829579297
14	20.927273834994427	27.134424960488545	32.717635548483514	19.220665656033518
15	19.898721656578257	23.6049141141012	37.2773112857619	19.219052943558648
16	25.426562449602734	23.989456444219858	30.376514605799315	20.20746650037809
17	25.975422261883	22.39125838162511	27.003795250024194	24.62952410646769
18	27.664469793895346	21.921421480613404	30.117405468170432	20.296703257320818
19	27.88845763762709	24.4257847638093	26.632871380804424	21.05288621775919
20	24.974644576089567	27.39066705371766	26.88051234083424	20.754176029358533
21	24.671275440539294	25.80967125752151	30.504994032963843	19.014059268975352
22	21.76015023312655	24.55963989922339	30.54782050868535	23.132389358964712
23	21.777710880075116	25.10240724215415	29.957209362333487	23.16267251543724
24	22.00761200288138	25.696602194005724	28.661305293639106	23.63448050947379
25	23.81152050115936	25.009228299161745	27.170263015485624	24.008988184193267
26	21.101267592005247	24.83666806435081	27.402135231316727	26.659929112327212
27	21.88146204929166	24.06184931531396	29.713331398078363	24.343357237316017
28	20.082391688438285	24.975540527464492	30.03300684865231	24.909060935444913
29	22.174079768342814	26.196722251489966	29.263205427315047	22.36599255285217
30	24.74689373658313	23.225209921407146	28.89658212469493	23.131314217314795
31	24.043751097540433	25.470643257249144	29.209806725369404	21.275798919841023
32	26.392039651224046	23.531266911082202	28.177491551178534	21.899201886515215
33	24.623790017668163	23.477689018861568	28.10026054265983	23.79826042081044
34	21.964964717434857	23.66494285622131	31.724025473689494	22.646066952654344
35	22.501818781291103	25.555937828142188	29.01413094508535	22.928112445481357
36	25.272817193665265	24.367906304989013	28.54536918572355	21.813907315622167
37	21.8993810767902	26.265352126809372	29.475187522622768	22.360079273777654
38	23.153354621138	26.143323549544316	27.769654485311772	22.933667344005908
39	22.903563377808357	22.850702246687668	28.279809198195196	25.96592517730878
40	25.5742152361907	22.26564599886035	29.665487594657264	22.494651170291686
41	21.62342805331269	22.43444323789659	28.830102532675344	27.11202617611537
42	22.75841925507019	22.94441876050503	30.86785433980927	23.42930764461551
43	22.067999125551456	22.427454817172162	30.743317098694416	24.76122895858196
44	21.754057763777045	22.885106779484865	29.676239011156387	25.684596445581704
45	21.220787505420503	22.86073690208685	27.932896825823466	27.985578766669178
46	22.773650428443947	23.47338845226192	28.648761974390126	25.104199144904012
47	20.702927610712713	23.707948522217805	31.467424999910403	24.12169886715908
48	21.74904043607745	24.454634398081947	29.216795146093833	24.579530019746766
49	23.422498414166064	22.08143839617536	31.62009511419796	22.87596807546061
50	21.61339339791351	22.862528804836703	30.466826504391953	25.057251292857835
51	21.4085789136052	23.319822386599434	27.80961391663352	27.461984783161846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	701.0
2	1149.0
3	1754.5
4	2360.0
5	1625.0
6	890.0
7	928.0
8	966.0
9	1020.5
10	1075.0
11	1161.5
12	1248.0
13	1236.5
14	1225.0
15	1278.0
16	1331.0
17	1344.0
18	1357.0
19	1336.0
20	1315.0
21	1535.5
22	1756.0
23	1872.5
24	1989.0
25	2299.0
26	3392.0
27	4175.0
28	4807.5
29	5440.0
30	6763.5
31	8087.0
32	8598.5
33	9110.0
34	11148.5
35	13187.0
36	13815.5
37	14444.0
38	16888.5
39	19333.0
40	21046.0
41	22759.0
42	25890.0
43	29021.0
44	32125.5
45	35230.0
46	40631.0
47	46032.0
48	50762.5
49	55493.0
50	54315.0
51	53137.0
52	47153.0
53	41169.0
54	38083.5
55	34998.0
56	33617.5
57	32237.0
58	31002.0
59	29767.0
60	26276.5
61	22786.0
62	20803.5
63	18821.0
64	16431.5
65	14042.0
66	11813.0
67	9584.0
68	8102.0
69	6620.0
70	5488.5
71	4357.0
72	3961.5
73	3566.0
74	2878.5
75	1844.0
76	1497.0
77	1161.0
78	825.0
79	571.0
80	317.0
81	263.5
82	210.0
83	131.5
84	53.0
85	35.0
86	17.0
87	21.5
88	26.0
89	16.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	558066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.130544601810474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91391536745495	23.009804390410007
2	10.303656719382284	6.415168901289488
3	3.73443458780477	3.4876494749460027
4	1.867067966288757	2.324913703966549
5	1.1331893075294892	1.7638400140170745
6	0.8201577103352033	1.5319173709265281
7	0.6298034779452296	1.372428768238453
8	0.5074245537549381	1.26371221621775
9	0.42839163447602036	1.2002458395688416
>10	6.279899848559822	47.09170999331967
>50	0.3242587014355523	6.147836993614614
>100	0.051442111279436945	2.624710926503828
>500	0.004046008752315266	0.8158250045086965
>1k	0.0023120050013230088	0.9502364024725247
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1785	0.31985464084893184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1263	0.22631731730655513	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1196	0.21431156888253358	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	1037	0.18582031515985564	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	942	0.16879723903624302	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	663	0.11880315231531755	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	657	0.11772801066540517	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	618	0.11073958994097471	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	584	0.10464712059147126	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	564	0.10106331509176333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.37570824956188E-4	0.0	0.0	0.19334630670924227	0.0
2	5.37570824956188E-4	0.0	0.0	0.9622517766715765	0.0
3	5.37570824956188E-4	0.0	0.0	1.3380137833159518	0.0
4	7.16761099941584E-4	0.0	0.0	1.797816028928478	0.0
5	7.16761099941584E-4	0.0	0.0	3.016847469654127	0.0
6	7.16761099941584E-4	0.0	0.0	3.7793020897169867	0.0
7	7.16761099941584E-4	0.0	0.0	4.466675984560966	0.0
8	7.16761099941584E-4	0.0	0.0	5.5366211164987655	0.0
9	7.16761099941584E-4	0.0	0.0	5.968290488938584	0.0
10	7.16761099941584E-4	0.0	0.0	6.938426637709518	0.0
11	7.16761099941584E-4	0.0	0.0	8.236122609153757	0.0
12	7.16761099941584E-4	0.0	0.0	9.329900047664614	0.0
13	8.9595137492698E-4	0.0	0.0	9.868366823995729	0.0
14	8.9595137492698E-4	0.0	0.0	10.119412399250267	0.0
15	8.9595137492698E-4	0.0	0.0	10.436220805424448	0.0
16	8.9595137492698E-4	0.0	0.0	11.10943866854458	0.0
17	8.9595137492698E-4	0.0	0.0	11.827454100411062	0.0
18	8.9595137492698E-4	0.0	0.0	12.820884984930098	0.0
19	8.9595137492698E-4	0.0	0.0	13.320646661864368	0.0
20	8.9595137492698E-4	0.0	0.0	13.925951410765036	0.0
21	8.9595137492698E-4	0.0	0.0	14.554909275963775	0.0
22	8.9595137492698E-4	0.0	0.0	15.229560661283791	0.0
23	8.9595137492698E-4	0.0	0.0	15.819096665985743	0.0
24	8.9595137492698E-4	0.0	0.0	16.314020205495407	0.0
25	8.9595137492698E-4	0.0	0.0	16.76665484010852	0.0
26	8.9595137492698E-4	0.0	0.0	17.22197732884641	0.0
27	8.9595137492698E-4	0.0	0.0	17.79556539907466	0.0
28	0.001075141649912376	0.0	0.0	18.266119061186313	0.0
29	0.001075141649912376	0.0	0.0	18.785233287819004	0.0
30	0.001075141649912376	0.0	0.0	19.407023542018326	0.0
31	0.0025086638497955438	0.0	0.0	19.85679113223167	0.0
32	0.00268785412478094	0.0	0.0	20.308888196019826	0.0
33	0.00268785412478094	0.0	0.0	20.81456315202861	0.0
34	0.00268785412478094	0.0	0.0	21.34442879516043	0.0
35	0.00268785412478094	0.0	0.0	21.86515573426799	0.0
36	0.00268785412478094	0.0	0.0	22.299333770557606	0.0
37	0.00268785412478094	0.0	0.0	22.792823787867384	0.0
38	0.00268785412478094	0.0	0.0	23.349747162521997	0.0
39	0.00268785412478094	0.0	0.0	24.298559668569666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAC	20	7.030163E-4	45.0	18
CGTATGA	20	7.030163E-4	45.0	40
CGCATAT	20	7.030163E-4	45.0	17
ATTACGG	25	3.8882175E-5	45.0	2
CACGATT	25	3.8882175E-5	45.0	27
TCGATTG	20	7.030163E-4	45.0	1
ACGATTG	25	3.8882175E-5	45.0	1
TGGCACG	25	3.8882175E-5	45.0	1
CATAGCG	20	7.030163E-4	45.0	1
CTCGCTA	20	7.030163E-4	45.0	44
ACTCGAC	20	7.030163E-4	45.0	41
TCGTAAG	20	7.030163E-4	45.0	12
TTCGGAC	25	3.8882175E-5	45.0	13
TTACCGG	20	7.030163E-4	45.0	2
TCGACCA	20	7.030163E-4	45.0	43
GATTACG	20	7.030163E-4	45.0	1
GCGTAAG	25	3.8882175E-5	45.0	1
TTAACGG	30	2.1634878E-6	44.999996	2
TTACGGG	90	0.0	42.5	3
ATACGAA	60	3.6379788E-12	41.249996	19
>>END_MODULE