FastQCFastQC Report
Sat 14 Jan 2017
SRR2935563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756479
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC97481.288601534213111Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC72060.9525710561694376No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG68630.9072294141674785No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67440.8914986404116969No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT28210.37291187197529607Illumina PCR Primer Index 7 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC23330.3084024804389811Illumina PCR Primer Index 7 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT13800.18242409901662837Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT12520.16550360287595559No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC11480.15175569976165895Illumina PCR Primer Index 7 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT10910.1442207913240156Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC10490.13866875352785735Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA9870.13047288820971897No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT9090.1201619608739965Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG8650.11434554032564023Illumina PCR Primer Index 7 (96% over 26bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8510.11249486106025416No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC8260.109190076657779Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC7890.10429899574211578No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7880.10416680436601676No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT7790.10297708198112572Illumina PCR Primer Index 7 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA207.031756E-445.036
CGACGCT207.031756E-445.035
ATATGCG207.031756E-445.01
ACGGAGT253.889537E-545.029
AATTCGG207.031756E-445.01
CATCGTT253.889537E-545.036
TCGAGTA207.031756E-445.026
ACGTACG253.889537E-545.01
CGTTTTT33650.042.459141
CCGATGA4750.041.21052618
CGAATAT1150.041.08695614
TCGTAAG403.4561344E-739.3751
TACGGCT8550.038.947377
GCCGATG659.094947E-1238.0769279
CGATGAA5150.038.0097119
GCATATC1250.037.828
ACGATAG301.13955306E-437.4999961
CAATGCG301.13955306E-437.4999961
TACCGGA301.13955306E-437.49999614
TGTTCGG552.746674E-936.8181842
Produced by FastQC (version 0.11.5)