##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756479
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07761484456277	33.0	31.0	34.0	30.0	34.0
2	32.12111638260943	33.0	31.0	34.0	30.0	34.0
3	32.09105606368452	33.0	31.0	34.0	30.0	34.0
4	35.76845755136626	37.0	35.0	37.0	35.0	37.0
5	35.818560726735306	37.0	35.0	37.0	35.0	37.0
6	35.74012100798568	37.0	35.0	37.0	35.0	37.0
7	35.99855382634548	37.0	35.0	37.0	35.0	37.0
8	35.77874468425429	37.0	35.0	37.0	35.0	37.0
9	37.61651017410926	39.0	37.0	39.0	35.0	39.0
10	37.28263970315105	39.0	37.0	39.0	34.0	39.0
11	37.26776817333991	39.0	37.0	39.0	34.0	39.0
12	37.13596411797287	39.0	37.0	39.0	34.0	39.0
13	37.019904055499225	39.0	37.0	39.0	33.0	39.0
14	38.050505037152384	40.0	37.0	41.0	33.0	41.0
15	38.143636505441656	40.0	37.0	41.0	33.0	41.0
16	38.06064279378542	40.0	37.0	41.0	33.0	41.0
17	38.07239328520686	40.0	37.0	41.0	33.0	41.0
18	37.994281401069955	40.0	37.0	41.0	33.0	41.0
19	37.799026807089156	40.0	36.0	41.0	33.0	41.0
20	37.78878858501029	40.0	35.0	41.0	33.0	41.0
21	37.80962194588349	40.0	35.0	41.0	33.0	41.0
22	37.93611322984511	40.0	36.0	41.0	34.0	41.0
23	37.94341812528834	40.0	36.0	41.0	34.0	41.0
24	37.87417363866016	40.0	35.0	41.0	34.0	41.0
25	37.646663026997444	40.0	35.0	41.0	33.0	41.0
26	37.64692608783588	40.0	35.0	41.0	33.0	41.0
27	37.66344207836569	40.0	35.0	41.0	33.0	41.0
28	37.5286068747447	40.0	35.0	41.0	33.0	41.0
29	37.46520656885386	40.0	35.0	41.0	33.0	41.0
30	37.29917023473222	39.0	35.0	41.0	33.0	41.0
31	37.18207907952501	39.0	35.0	41.0	32.0	41.0
32	37.06798866855524	39.0	35.0	41.0	32.0	41.0
33	36.88735444077099	39.0	35.0	41.0	31.0	41.0
34	36.664884286278934	39.0	35.0	41.0	31.0	41.0
35	36.58097581030009	39.0	35.0	41.0	31.0	41.0
36	36.46561900594729	39.0	35.0	41.0	30.0	41.0
37	36.40659423460532	39.0	35.0	41.0	30.0	41.0
38	36.24477348346748	39.0	35.0	41.0	30.0	41.0
39	36.13523177774928	39.0	35.0	41.0	30.0	41.0
40	36.013208562299816	39.0	35.0	41.0	29.0	41.0
41	35.81476683424127	39.0	35.0	41.0	28.0	41.0
42	35.87513599187816	39.0	35.0	41.0	29.0	41.0
43	35.84551587023566	39.0	35.0	41.0	29.0	41.0
44	35.75024290165358	38.0	35.0	41.0	29.0	41.0
45	35.749727355286794	38.0	35.0	41.0	29.0	41.0
46	35.60896336844777	38.0	35.0	40.0	28.0	41.0
47	35.45578528947929	38.0	35.0	40.0	28.0	41.0
48	35.401942420080395	38.0	35.0	40.0	27.0	41.0
49	35.38932739705927	38.0	35.0	40.0	27.0	41.0
50	35.25099573154046	38.0	35.0	40.0	27.0	41.0
51	34.04224968571501	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	27.0
10	18.0
11	20.0
12	23.0
13	17.0
14	33.0
15	54.0
16	60.0
17	123.0
18	265.0
19	576.0
20	993.0
21	1705.0
22	2528.0
23	3567.0
24	4682.0
25	6720.0
26	8951.0
27	9968.0
28	10185.0
29	10632.0
30	12230.0
31	15034.0
32	19668.0
33	27923.0
34	51302.0
35	59935.0
36	55491.0
37	79483.0
38	135864.0
39	238221.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86638624469417	18.86014020217349	22.531623481947285	14.741850071185056
2	33.446665406442214	27.602616860481255	22.831697905692028	16.119019827384502
3	29.699436468163693	27.26923020995956	27.245435762261742	15.785897559615005
4	26.876093057441118	23.506402689301357	32.06989222437107	17.54761202888646
5	27.36321827836596	26.96373593979476	26.509394180142476	19.163651601696806
6	24.142904165218066	34.60757007134369	26.98237492382472	14.267150839613526
7	74.8909090668743	5.521105014151087	15.042453260434197	4.545532658540422
8	74.88905838760891	8.311664963601105	11.998085868874087	4.8011907799159
9	69.63273269978413	6.5907976295442445	13.654311620018532	10.122158050653091
10	40.69617266308781	23.05615886230814	21.089547760083228	15.158120714520825
11	32.14444816049091	23.23884734407697	26.19702595842052	18.419678537011603
12	27.42283658898661	20.551528859360275	31.17654290469398	20.849091646959135
13	25.99794574601542	21.90847333501657	33.59220811152722	18.501372807440788
14	20.904876407672916	26.18988762411118	32.50149706733432	20.403738900881585
15	19.87127203795479	23.35517575504409	36.6946075171948	20.078944689806328
16	23.60779347476929	23.744479357655663	30.203085611100907	22.44464155647414
17	23.74738756792984	23.517242382141472	30.35880705214553	22.37656299778315
18	24.83466163634417	22.888143623286304	30.14782961589152	22.12936512447801
19	24.150174690903516	25.841034582585902	27.28919110775051	22.71959961876007
20	24.75984131747213	26.358299437261312	29.866790750305032	19.015068494961525
21	23.462647343812584	27.174581184672675	30.64870273993065	18.71406873158409
22	22.011318225621597	22.1703444510687	31.965593228628954	23.85274409468075
23	22.336376819449054	25.77361698077541	30.72616688632467	21.163839313450868
24	23.08576973055432	24.80624049048288	29.225398193472657	22.882591585490143
25	22.32091042844547	27.952263050263127	27.531894474268288	22.194932047023116
26	20.433085386375563	25.280807530678313	29.875779763879763	24.410327319066358
27	23.570383315333274	24.45659430070101	29.103253361957172	22.869769022008544
28	20.548885031838292	26.5193085333499	29.925880295421287	23.005926139390517
29	23.391131809343022	26.12326317055728	28.44071018494895	22.044894835150743
30	24.39671160732816	26.084266714608074	28.624588389102673	20.894433288961096
31	24.517137950954357	27.055080180679177	26.56293168746257	21.864850180903897
32	27.351321054517047	26.309388628104678	25.43520705796195	20.90408325941632
33	24.561157679195325	24.756404341693557	25.871438599088677	24.810999380022448
34	21.798490110102197	28.259607999693316	28.41440410110525	21.527497789099233
35	23.067262937900455	26.58500764727111	28.075994178291797	22.27173523653664
36	23.234088454537403	28.803178938212433	24.969496839965156	22.993235767285015
37	24.23292649234149	27.105841669101196	27.454298136498174	21.206933702059143
38	22.49857563792253	27.210933813099903	26.099600914235555	24.190889634742007
39	24.0458756951614	25.49284249794112	26.469736767312774	23.991545039584707
40	23.193109127946713	23.900200798700293	29.54411160124736	23.362578472105636
41	20.798462349913216	26.394916448440735	28.22550262465977	24.581118576986274
42	21.849648172652515	25.014706290591015	30.64777740029796	22.487868136458513
43	22.933088691159966	24.382567130085565	27.5968004399329	25.087543738821566
44	22.45812507683624	24.72771881308007	27.951469902006533	24.862686208077157
45	21.81263458734479	24.15863493897385	27.45562005025916	26.573110423422197
46	24.0193052285655	25.717039071805033	26.75341946042124	23.510236239208226
47	20.428326496836	25.215504990885407	31.477939242199714	22.87822927007888
48	21.706088338208993	26.268805875642286	28.332842022052162	23.69226376409656
49	22.388063647503763	24.129024070727674	30.270767595663596	23.212144686104967
50	21.253861640574293	23.960876640329737	29.304845210508155	25.480416508587812
51	20.68490995784417	24.95852495574894	27.383972324413502	26.97259276199339
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	861.5
2	1415.0
3	6138.5
4	10862.0
5	7129.0
6	3396.0
7	3351.0
8	3306.0
9	3281.0
10	3256.0
11	3183.5
12	3111.0
13	3022.0
14	2933.0
15	2819.5
16	2706.0
17	2710.5
18	2715.0
19	2550.5
20	2386.0
21	2758.5
22	3131.0
23	3117.0
24	3103.0
25	3837.5
26	5099.5
27	5627.0
28	6734.5
29	7842.0
30	9216.5
31	10591.0
32	11744.5
33	12898.0
34	15306.5
35	17715.0
36	19442.0
37	21169.0
38	22373.5
39	23578.0
40	26265.0
41	28952.0
42	32374.0
43	35796.0
44	39035.5
45	42275.0
46	64529.0
47	86783.0
48	75776.0
49	64769.0
50	64138.5
51	63508.0
52	56369.5
53	49231.0
54	46099.0
55	42967.0
56	41156.0
57	39345.0
58	37731.5
59	36118.0
60	32874.0
61	29630.0
62	27325.0
63	25020.0
64	22463.0
65	19906.0
66	16369.5
67	12833.0
68	11825.5
69	10818.0
70	9159.0
71	7500.0
72	6711.5
73	5923.0
74	4767.5
75	2870.0
76	2128.0
77	1688.5
78	1249.0
79	1015.5
80	782.0
81	595.0
82	408.0
83	268.0
84	128.0
85	140.5
86	153.0
87	85.0
88	17.0
89	11.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	756479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.658766061188537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36349346843896	22.79892949741592
2	8.918408416935916	5.468547945859461
3	3.4035263996458087	3.1304375900946035
4	1.8671574561457336	2.2897897458950376
5	1.2752692465585806	1.9549090747633846
6	0.9587573458168668	1.7636590304907214
7	0.7424099070173581	1.593296016252774
8	0.6519305915618124	1.598990999586069
9	0.5681903403066455	1.567801324951969
>10	6.977718810684864	45.45288141198151
>50	0.2367773300400962	4.384644206971283
>100	0.027270515135519872	1.4271758759343947
>500	0.0043286531961142655	1.04350789589661
>1k	0.0030300572372799857	1.4696434489582932
>5k	0.001731461278445706	4.055785934948022
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	9748	1.288601534213111	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	7206	0.9525710561694376	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	6863	0.9072294141674785	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6744	0.8914986404116969	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	2821	0.37291187197529607	Illumina PCR Primer Index 7 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2333	0.3084024804389811	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1380	0.18242409901662837	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	1252	0.16550360287595559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	1148	0.15175569976165895	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1091	0.1442207913240156	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1049	0.13866875352785735	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	987	0.13047288820971897	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	909	0.1201619608739965	Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	865	0.11434554032564023	Illumina PCR Primer Index 7 (96% over 26bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	851	0.11249486106025416	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	826	0.109190076657779	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	789	0.10429899574211578	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	788	0.10416680436601676	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	779	0.10297708198112572	Illumina PCR Primer Index 7 (96% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.643827521980121E-4	0.0	0.0	0.10826473702508596	0.0
2	2.643827521980121E-4	0.0	0.0	0.6670376837955846	0.0
3	2.643827521980121E-4	0.0	0.0	0.9726641453364865	0.0
4	2.643827521980121E-4	0.0	0.0	1.656093559768348	0.0
5	2.643827521980121E-4	0.0	0.0	3.654430592257022	0.0
6	2.643827521980121E-4	0.0	0.0	4.53257790368272	0.0
7	2.643827521980121E-4	0.0	0.0	5.309070046888281	0.0
8	2.643827521980121E-4	0.0	0.0	6.2753890061720154	0.0
9	2.643827521980121E-4	0.0	0.0	6.71360341794022	0.0
10	2.643827521980121E-4	0.0	0.0	8.310210858464016	0.0
11	2.643827521980121E-4	0.0	0.0	9.171173290996842	0.0
12	3.9657412829701815E-4	0.0	0.0	10.797259408390715	0.0
13	3.9657412829701815E-4	0.0	0.0	11.188281498891575	0.0
14	3.9657412829701815E-4	0.0	0.0	11.395425385238719	0.0
15	5.287655043960242E-4	0.0	0.0	11.7303983322736	0.0
16	6.609568804950303E-4	0.0	0.0	12.139795024052221	0.0
17	6.609568804950303E-4	0.0	0.0	12.576951904811635	0.0
18	6.609568804950303E-4	0.0	0.0	13.11470642278239	0.0
19	6.609568804950303E-4	0.0	0.0	13.60156726095503	0.0
20	7.931482565940363E-4	0.0	0.0	13.96866271238197	0.0
21	7.931482565940363E-4	0.0	0.0	14.346862239401226	0.0
22	7.931482565940363E-4	0.0	0.0	14.784944459793332	0.0
23	7.931482565940363E-4	0.0	0.0	15.198306892854925	0.0
24	9.253396326930423E-4	0.0	0.0	15.536320241540082	0.0
25	9.253396326930423E-4	0.0	0.0	15.837320004917519	0.0
26	0.0010575310087920485	0.0	0.0	16.143739614715017	0.0
27	0.0010575310087920485	0.0	0.0	16.511099448894154	0.0
28	0.0010575310087920485	0.0	0.0	16.82941628254056	0.0
29	0.0010575310087920485	0.0	0.0	17.18051657745952	0.0
30	0.0010575310087920485	0.0	0.0	17.656008957287643	0.0
31	0.0010575310087920485	0.0	0.0	18.016098265781338	0.0
32	0.0010575310087920485	0.0	0.0	18.35860612125386	0.0
33	0.0011897223848910544	0.0	0.0	18.71089613855771	0.0
34	0.0011897223848910544	0.0	0.0	19.059088223202494	0.0
35	0.0013219137609900605	0.0	0.0	19.435569262332464	0.0
36	0.0014541051370890667	0.0	0.0	19.796980484587145	0.0
37	0.0014541051370890667	0.0	0.0	20.15971362060282	0.0
38	0.0015862965131880726	0.0	0.0	20.586030808522114	0.0
39	0.0015862965131880726	0.0	0.0	21.21856654315586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.031756E-4	45.0	36
CGACGCT	20	7.031756E-4	45.0	35
ATATGCG	20	7.031756E-4	45.0	1
ACGGAGT	25	3.889537E-5	45.0	29
AATTCGG	20	7.031756E-4	45.0	1
CATCGTT	25	3.889537E-5	45.0	36
TCGAGTA	20	7.031756E-4	45.0	26
ACGTACG	25	3.889537E-5	45.0	1
CGTTTTT	3365	0.0	42.45914	1
CCGATGA	475	0.0	41.210526	18
CGAATAT	115	0.0	41.086956	14
TCGTAAG	40	3.4561344E-7	39.375	1
TACGGCT	855	0.0	38.94737	7
GCCGATG	65	9.094947E-12	38.076927	9
CGATGAA	515	0.0	38.00971	19
GCATATC	125	0.0	37.8	28
ACGATAG	30	1.13955306E-4	37.499996	1
CAATGCG	30	1.13955306E-4	37.499996	1
TACCGGA	30	1.13955306E-4	37.499996	14
TGTTCGG	55	2.746674E-9	36.818184	2
>>END_MODULE