##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935562.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950659
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10686376503036	33.0	31.0	34.0	30.0	34.0
2	32.144137908545545	33.0	31.0	34.0	30.0	34.0
3	32.10216176357663	33.0	31.0	34.0	30.0	34.0
4	35.782316266926415	37.0	35.0	37.0	35.0	37.0
5	35.866499975280306	37.0	35.0	37.0	35.0	37.0
6	35.810855417136956	37.0	35.0	37.0	35.0	37.0
7	36.08254905281494	37.0	35.0	37.0	35.0	37.0
8	35.92741350999675	37.0	35.0	37.0	35.0	37.0
9	37.74649269611922	39.0	38.0	39.0	35.0	39.0
10	37.35724060888289	39.0	37.0	39.0	34.0	39.0
11	37.353859796204524	39.0	37.0	39.0	34.0	39.0
12	37.22118867017511	39.0	37.0	39.0	34.0	39.0
13	37.190753992756605	39.0	37.0	39.0	34.0	39.0
14	38.21852525458655	40.0	38.0	41.0	33.0	41.0
15	38.374480228978	40.0	38.0	41.0	34.0	41.0
16	38.17376788101728	40.0	37.0	41.0	33.0	41.0
17	38.25056408238916	40.0	37.0	41.0	34.0	41.0
18	38.12854030730262	40.0	37.0	41.0	34.0	41.0
19	37.93381854061236	40.0	37.0	41.0	34.0	41.0
20	38.05404671917059	40.0	36.0	41.0	34.0	41.0
21	37.941191320967874	40.0	36.0	41.0	34.0	41.0
22	38.06979263858018	40.0	36.0	41.0	34.0	41.0
23	38.08965780579577	40.0	36.0	41.0	34.0	41.0
24	38.02049736025221	40.0	36.0	41.0	34.0	41.0
25	37.74981986180113	40.0	35.0	41.0	33.0	41.0
26	37.84433324672674	40.0	35.0	41.0	34.0	41.0
27	37.86509358245175	40.0	35.0	41.0	34.0	41.0
28	37.75161651023132	40.0	35.0	41.0	33.0	41.0
29	37.71449910009793	40.0	35.0	41.0	33.0	41.0
30	37.51972473831311	40.0	35.0	41.0	33.0	41.0
31	37.44626937734771	40.0	35.0	41.0	33.0	41.0
32	37.33571869618864	40.0	35.0	41.0	33.0	41.0
33	37.201159406264495	40.0	35.0	41.0	33.0	41.0
34	37.018063259275934	40.0	35.0	41.0	32.0	41.0
35	36.87665608803998	40.0	35.0	41.0	31.0	41.0
36	36.74003296660527	39.0	35.0	41.0	31.0	41.0
37	36.690645120910865	39.0	35.0	41.0	31.0	41.0
38	36.538397048784056	39.0	35.0	41.0	31.0	41.0
39	36.45826000700567	39.0	35.0	41.0	31.0	41.0
40	36.3494091993028	39.0	35.0	41.0	30.0	41.0
41	36.19323858502365	39.0	35.0	41.0	30.0	41.0
42	36.166171045558926	39.0	35.0	41.0	30.0	41.0
43	36.09954883927886	39.0	35.0	41.0	30.0	41.0
44	35.97076764644315	39.0	35.0	41.0	30.0	41.0
45	35.94980218984936	38.0	35.0	41.0	30.0	41.0
46	35.846206683995	38.0	35.0	41.0	29.0	41.0
47	35.781532600017464	38.0	35.0	40.0	29.0	41.0
48	35.66050392412001	38.0	35.0	40.0	29.0	41.0
49	35.627985429055	38.0	35.0	40.0	29.0	41.0
50	35.5154150962648	38.0	35.0	40.0	28.0	41.0
51	34.42932113407647	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	23.0
10	40.0
11	28.0
12	20.0
13	27.0
14	48.0
15	65.0
16	91.0
17	175.0
18	317.0
19	530.0
20	871.0
21	1392.0
22	2035.0
23	3029.0
24	4616.0
25	7322.0
26	10201.0
27	11783.0
28	12571.0
29	13183.0
30	14391.0
31	17432.0
32	22886.0
33	32372.0
34	61457.0
35	71811.0
36	69748.0
37	103599.0
38	182400.0
39	306032.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2214053619647	19.385605143379486	23.13279525045258	16.260194244203234
2	35.32013056206274	22.054280241390444	24.414748085275583	18.210841111271233
3	30.877422924518676	22.705933462997773	29.54119195210901	16.87545166037454
4	27.409407579373884	24.62670631635529	28.619305134648705	19.344580969622125
5	24.866960708308657	28.077049709727675	27.682376120144024	19.373613461819644
6	24.966681007595785	32.05502709173321	27.542052407856023	15.436239492814984
7	77.20234069208833	5.850152367988942	11.976534172610789	4.970972767311938
8	78.68257703340524	4.6789648023108175	11.666012734324296	4.972445429959638
9	72.96233454898129	6.58069823143735	14.129987724304929	6.326979495276435
10	39.482190775030794	23.274696815577407	21.46248023739322	15.780632171998581
11	31.33805076268147	24.090236351835937	26.222967436273155	18.348745449209446
12	26.938786673244557	22.30189794658232	30.32570038257672	20.433614997596404
13	25.012859500620095	22.454002960051923	32.39931458072768	20.133822958600298
14	21.02604614272836	24.765872936562953	33.0749511654547	21.13312975525399
15	20.12446103176849	23.84303940740055	34.86276361976271	21.16973594106825
16	24.19731996436156	24.167656331029317	31.434930926862314	20.200092777746807
17	24.35605195974582	24.02344058174382	29.940914670770486	21.679592787739875
18	25.830292460282816	22.860142280249807	30.756033446272536	20.553531813194848
19	24.759877095783033	25.660936255797296	28.447213985246023	21.131972663173652
20	25.05525114683604	26.34467248508666	28.443321948248528	20.156754419828772
21	23.760149538372854	26.335310558254854	30.492847593090687	19.411692310281605
22	23.92350990207845	23.431114626800987	30.204416094519697	22.440959376600862
23	22.113397127676695	25.461495657223043	30.51567386413004	21.90943335097022
24	22.26423985887684	24.206997461760736	30.937170951939652	22.591591727422767
25	23.35748149441598	25.363458400961857	28.5491432785047	22.72991682611746
26	21.98811561243306	24.880740623083568	29.179232511342136	23.95191125314124
27	22.1865043091161	23.548717258238757	30.5679533881234	23.696825044521745
28	20.880673301362528	25.003182003220925	30.105642506934664	24.010502188481887
29	22.215326420935373	24.66478516481725	29.81268783023145	23.30720058401593
30	23.304465639098773	22.982373280008918	30.720268781971242	22.992892298921063
31	23.095031972557983	23.650541361308314	30.756349016839895	22.498077649293805
32	24.105173358691182	23.192332897495316	30.329487229385087	22.37300651442841
33	23.669685975728417	22.71750438380113	30.23544720031052	23.37736244015993
34	21.438707254651774	23.74721114511092	32.35744888545735	22.45663271477996
35	21.774684718705657	24.420849116244625	31.434930926862314	22.369535238187403
36	23.565337308119947	26.11809281771908	28.6776856896111	21.638884184549877
37	23.02465973603574	25.387231383703305	30.01686198731617	21.57124689294479
38	23.577223799490667	25.644842156861714	28.874812104024684	21.903121939622935
39	23.668213313080717	23.32539848673394	28.55577026041935	24.450617939765994
40	25.30444670486473	22.324303456865184	30.17264865740502	22.19860118086506
41	22.93261832055448	23.44415821025205	29.12947755188769	24.493745917305784
42	24.09486472015728	23.86323592371187	29.230460133444275	22.811439222686577
43	23.42175269996918	23.183076160852632	30.12583902324598	23.26933211593221
44	23.52694288909062	22.876025998807144	29.327761058381608	24.26927005372063
45	22.390047325066085	22.882547790532673	29.37814715897078	25.349257725430462
46	22.61094672222111	23.37420673448629	28.816326358873162	25.198520184419436
47	21.535166658076136	24.555597748509193	30.793901914356255	23.115333679058423
48	21.832328942344205	24.019443354557207	30.448562523470564	23.699665179628028
49	23.799385478915152	22.7634724964472	30.179486019697915	23.25765600493973
50	21.32078905264664	23.545561552565115	30.74183277074114	24.39181662404711
51	21.440074727110353	22.781460018786966	29.740106599737654	26.038358654365023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	477.0
1	979.0
2	1481.0
3	8015.0
4	14549.0
5	9347.0
6	4145.0
7	4061.0
8	3977.0
9	4025.0
10	4073.0
11	3937.5
12	3802.0
13	3838.0
14	3874.0
15	3614.0
16	3354.0
17	3193.5
18	3033.0
19	3038.0
20	3043.0
21	3320.0
22	3597.0
23	4030.0
24	4463.0
25	4660.0
26	6087.0
27	7317.0
28	8241.0
29	9165.0
30	10954.5
31	12744.0
32	14652.5
33	16561.0
34	19565.5
35	22570.0
36	24057.5
37	25545.0
38	27697.0
39	29849.0
40	33305.0
41	36761.0
42	40552.5
43	44344.0
44	49404.0
45	54464.0
46	63945.0
47	73426.0
48	80592.5
49	87759.0
50	87699.0
51	87639.0
52	79700.0
53	71761.0
54	65757.5
55	59754.0
56	54993.5
57	50233.0
58	47171.0
59	44109.0
60	41406.5
61	38704.0
62	35589.0
63	32474.0
64	28863.5
65	25253.0
66	22331.0
67	19409.0
68	16802.0
69	14195.0
70	12343.0
71	10491.0
72	8826.5
73	7162.0
74	5968.5
75	3758.0
76	2741.0
77	2096.5
78	1452.0
79	1054.5
80	657.0
81	483.0
82	309.0
83	236.5
84	164.0
85	120.5
86	77.0
87	52.0
88	27.0
89	25.5
90	24.0
91	14.5
92	5.0
93	7.5
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	950659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.56376424192108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70914147246181	22.949741996692712
2	9.126136225101652	5.761104244975283
3	3.553554423605887	3.3649066214259595
4	1.9953479907564076	2.5192277424330474
5	1.4198956258462692	2.2408625391173316
6	1.0974917037922536	2.078458163757779
7	0.8639479702311239	1.9088615034763015
8	0.7729926313573805	1.9518845741525257
9	0.6592014372564995	1.8726190878149716
>10	7.644885691469488	48.15711224284999
>50	0.11645948156712882	2.2906040209748424
>100	0.03557546503881232	2.042080972936188
>500	0.0020137055682313263	0.39439059305048485
>1k	0.0030205583523469897	1.4687686201034038
>5k	3.3561759470522107E-4	0.9993770762391281
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9434	0.9923642441716747	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	2330	0.2450931406529576	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	2018	0.21227380164706797	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1660	0.1746157139415921	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1491	0.15683857198006856	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	1418	0.1491596881742034	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1380	0.1451624609875886	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	1264	0.13296039904950147	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	1209	0.1271749386478222	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1095	0.11518325708797791	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.363313238500872E-4	0.0	0.0	0.140008141720638	0.0
2	7.363313238500872E-4	0.0	0.0	0.5881183473779767	0.0
3	7.363313238500872E-4	0.0	0.0	0.9650147950000999	0.0
4	7.363313238500872E-4	0.0	0.0	1.395558239074158	0.0
5	7.363313238500872E-4	0.0	0.0	2.454297492581462	0.0
6	7.363313238500872E-4	0.0	0.0	3.6365300281173374	0.0
7	7.363313238500872E-4	0.0	0.0	4.590289472881444	0.0
8	7.363313238500872E-4	0.0	0.0	5.8711904058132305	0.0
9	7.363313238500872E-4	0.0	0.0	6.591848391484223	0.0
10	7.363313238500872E-4	0.0	0.0	7.698764751609147	0.0
11	8.415215129715281E-4	0.0	0.0	8.835870696011924	0.0
12	9.467117020929692E-4	0.0	0.0	9.670870417257923	0.0
13	9.467117020929692E-4	0.0	0.0	10.081427725398907	0.0
14	9.467117020929692E-4	0.0	0.0	10.314318804113778	0.0
15	9.467117020929692E-4	0.0	0.0	10.587287344883917	0.0
16	9.467117020929692E-4	0.0	0.0	11.036028691675984	0.0
17	9.467117020929692E-4	0.0	0.0	11.536208040948436	0.0
18	9.467117020929692E-4	0.0	0.0	12.125167909839385	0.0
19	9.467117020929692E-4	0.0	0.0	12.493859522710036	0.0
20	9.467117020929692E-4	0.0	0.0	12.86581203144345	0.0
21	0.0010519018912144103	0.0	0.0	13.309399058968568	0.0
22	0.0010519018912144103	0.0	0.0	13.754248368763141	0.0
23	0.0010519018912144103	0.0	0.0	14.204777948770275	0.0
24	0.0010519018912144103	0.0	0.0	14.595559501356428	0.0
25	0.0011570920803358512	0.0	0.0	14.938900278648811	0.0
26	0.0011570920803358512	0.0	0.0	15.27529850345918	0.0
27	0.0011570920803358512	0.0	0.0	15.651774190324817	0.0
28	0.0011570920803358512	0.0	0.0	16.01257653901136	0.0
29	0.0011570920803358512	0.0	0.0	16.39799339195232	0.0
30	0.0011570920803358512	0.0	0.0	16.869035058838133	0.0
31	0.0013674724585787334	0.0	0.0	17.253294819698755	0.0
32	0.0013674724585787334	0.0	0.0	17.652491587414627	0.0
33	0.0013674724585787334	0.0	0.0	18.031176268251812	0.0
34	0.0014726626477001743	0.0	0.0	18.392188997316598	0.0
35	0.0014726626477001743	0.0	0.0	18.7807615559312	0.0
36	0.0015778528368216153	0.0	0.0	19.157763193742447	0.0
37	0.0015778528368216153	0.0	0.0	19.54717727387002	0.0
38	0.0015778528368216153	0.0	0.0	19.979824521726506	0.0
39	0.0015778528368216153	0.0	0.0	20.605811337188204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	115	0.0	45.000004	12
CGTTACA	20	7.032671E-4	45.0	32
TAGTACG	45	3.8380676E-10	45.0	1
GCTACGA	120	0.0	44.999996	10
CTACGAA	120	0.0	43.124996	11
CGTTTTT	4325	0.0	42.71098	1
CTATGCG	70	0.0	41.785713	1
GCGTAAG	70	0.0	41.785713	1
CGAATAT	130	0.0	39.80769	14
AGTACGG	85	0.0	39.705883	2
ACGTTAG	40	3.4573895E-7	39.375	1
CGTAAGG	150	0.0	39.000004	2
TATAGCG	35	6.2471518E-6	38.571426	1
CATCGTT	30	1.1397735E-4	37.499996	36
CGTTTAG	30	1.1397735E-4	37.499996	30
TATCGCG	30	1.1397735E-4	37.499996	1
GACGGGA	285	0.0	37.105263	4
CGTTAGG	165	0.0	36.81818	2
TTCGTAG	25	0.0021069255	36.000004	1
TGACCGT	25	0.0021069255	36.000004	34
>>END_MODULE