##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935561.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503474
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191223379956064	31.0	31.0	33.0	28.0	34.0
2	31.198576291923715	31.0	31.0	34.0	28.0	34.0
3	31.022972387849222	31.0	31.0	34.0	27.0	34.0
4	34.856616230430966	35.0	35.0	37.0	32.0	37.0
5	34.855088842720775	35.0	35.0	37.0	33.0	37.0
6	35.014840885527356	37.0	35.0	37.0	32.0	37.0
7	35.516640382621546	37.0	35.0	37.0	33.0	37.0
8	35.187892522751916	37.0	35.0	37.0	32.0	37.0
9	37.1014014626376	39.0	37.0	39.0	33.0	39.0
10	36.42299503052789	38.0	35.0	39.0	32.0	39.0
11	36.6417670028641	39.0	35.0	39.0	32.0	39.0
12	36.79156619805591	39.0	35.0	39.0	33.0	39.0
13	36.836287474626296	39.0	37.0	39.0	33.0	39.0
14	37.90460083340947	40.0	37.0	41.0	33.0	41.0
15	38.108386927626846	40.0	37.0	41.0	33.0	41.0
16	38.05629883568963	40.0	37.0	41.0	33.0	41.0
17	37.94734981349583	40.0	37.0	41.0	33.0	41.0
18	37.93570273738068	39.0	37.0	41.0	33.0	41.0
19	37.821398920301746	39.0	37.0	41.0	33.0	41.0
20	37.86051514080171	39.0	36.0	41.0	33.0	41.0
21	37.82640017160767	40.0	36.0	41.0	33.0	41.0
22	37.940707961086375	40.0	37.0	41.0	33.0	41.0
23	37.978469593265984	40.0	37.0	41.0	34.0	41.0
24	37.94877789121186	40.0	36.0	41.0	33.0	41.0
25	37.52388206739573	39.0	36.0	41.0	33.0	41.0
26	37.61048236850364	39.0	36.0	41.0	33.0	41.0
27	37.655018928484886	40.0	36.0	41.0	33.0	41.0
28	37.55020716064782	40.0	36.0	41.0	33.0	41.0
29	37.55937943170849	40.0	36.0	41.0	33.0	41.0
30	37.30096489590326	39.0	36.0	41.0	32.0	41.0
31	37.30192621664674	39.0	36.0	41.0	32.0	41.0
32	37.278195100442126	39.0	36.0	41.0	32.0	41.0
33	37.27718412470157	40.0	36.0	41.0	32.0	41.0
34	37.20692429003285	40.0	36.0	41.0	32.0	41.0
35	37.20428463038807	40.0	36.0	41.0	32.0	41.0
36	37.05519252235468	40.0	35.0	41.0	32.0	41.0
37	37.06258118592022	40.0	35.0	41.0	32.0	41.0
38	36.903675264263896	39.0	35.0	41.0	31.0	41.0
39	36.822687964025945	39.0	35.0	41.0	31.0	41.0
40	36.72064098642631	39.0	35.0	41.0	31.0	41.0
41	36.5369234558289	39.0	35.0	41.0	30.0	41.0
42	36.579404298931024	39.0	35.0	41.0	31.0	41.0
43	36.5516054453656	39.0	35.0	41.0	31.0	41.0
44	36.53917580649646	39.0	35.0	41.0	31.0	41.0
45	36.538063534561864	39.0	35.0	41.0	31.0	41.0
46	36.396316393696594	39.0	35.0	41.0	31.0	41.0
47	36.35125746314606	39.0	35.0	41.0	31.0	41.0
48	36.28385775630916	39.0	35.0	40.0	31.0	41.0
49	36.23689803247039	39.0	35.0	40.0	30.0	41.0
50	36.12375812852302	38.0	35.0	40.0	30.0	41.0
51	34.98495850828444	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	38.0
10	38.0
11	31.0
12	20.0
13	34.0
14	25.0
15	40.0
16	64.0
17	102.0
18	193.0
19	351.0
20	623.0
21	1000.0
22	1478.0
23	2057.0
24	2695.0
25	3311.0
26	4299.0
27	4951.0
28	5370.0
29	6390.0
30	7693.0
31	9987.0
32	13280.0
33	19026.0
34	33344.0
35	36810.0
36	41934.0
37	63726.0
38	110727.0
39	133797.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30814699468096	20.371856342134848	23.598438052411844	11.721558610772354
2	33.10399345348519	25.057500486618974	24.06996190468624	17.768544155209604
3	33.252561204749405	24.232035815156294	25.936592554928357	16.578810425165948
4	29.490500005958598	24.834450239734327	26.257959696031968	19.417090058275104
5	26.306224353194008	30.24724216146216	23.442521361579743	20.004012123764088
6	25.97373449274441	35.83879207267903	23.397434624230844	14.790038810345719
7	79.05611809150027	6.175691296869352	9.94410833528643	4.824082276343962
8	79.68077795477025	7.439510282556795	8.1553367204662	4.724375042206748
9	73.52673623662791	8.281063173073486	10.213238419461582	7.978962170837025
10	40.394538744801125	28.740113690081316	16.74386363546082	14.121483929656744
11	30.543185944060667	26.24246733694292	24.459257081795684	18.75508963720073
12	28.913111699909035	22.45319519975212	28.25170713879962	20.381985961539225
13	26.411492947004216	23.253435132698012	29.405689270945473	20.9293826493523
14	20.257252608873547	28.622332037006874	27.97264605520841	23.147769298911165
15	19.744813039004995	26.892153318741386	32.368702256720304	20.994331385533314
16	22.417244981865995	25.275386613807267	30.24843388139209	22.05893452293465
17	22.028148424744874	25.728041567191156	28.3210255147237	23.92278449334027
18	22.913397712692216	26.536027679681574	27.49496498329606	23.055609624330152
19	25.15561876084962	27.61691765612524	25.6072806142919	21.620182968733243
20	27.454446505678547	25.290283112931355	27.312234594040607	19.943035787349494
21	25.963604873340035	26.96067721471218	26.524507720358947	20.55121019158884
22	23.841747538105245	24.82849164008469	26.91320703750343	24.41655378430664
23	24.275732212586945	27.108847725999752	26.964649614478603	21.650770446934697
24	23.88603979550086	25.069020445941597	28.134918585666785	22.910021172890755
25	23.38114778518851	27.57322125869459	26.087940986029068	22.95768997008783
26	22.307606748312722	27.054227229211442	27.042111409923848	23.59605461255199
27	23.79030496113007	27.053631369246478	27.230204538863973	21.925859130759484
28	21.273789709101166	28.058648510151468	28.53374752221565	22.13381425853172
29	22.79938983939588	25.853966639786762	27.464576125082925	23.88206739573444
30	22.54952589408788	25.7490952859532	28.56254742052221	23.138831399436715
31	22.879036454712658	26.96782753429174	25.966782793153172	24.18635321784243
32	24.031032386975294	27.01748253137203	26.13839046306264	22.813094618590036
33	23.661797828686286	26.483989242741433	26.70842982954433	23.145783099027952
34	22.6089132705959	26.69710849021002	27.18015230180704	23.513825937387033
35	22.83315523741047	24.46203776163218	26.593428856306385	26.111378144650963
36	22.656979307769618	27.680078812411367	26.308806413042184	23.354135466776835
37	22.782904380365224	27.079849207704864	27.622479015798234	22.514767396131678
38	22.10203506040034	26.302251953427586	26.87626371967569	24.719449266496383
39	23.380154685246904	25.063459086268608	26.933466276312185	24.622919952172307
40	23.170809217556418	24.530363037614652	29.432105729392184	22.866722015436746
41	21.12681091774352	26.36203656991225	28.197086642011303	24.314065870332925
42	22.567401693036782	25.614828173848103	27.62704727552962	24.190722857585495
43	23.904511454414727	25.434878464429143	26.922144936977876	23.73846514417825
44	22.29688126894338	25.787428943699176	27.780183286525222	24.135506500832218
45	22.524897015536055	24.426087543746053	26.605147435617333	26.443868005100562
46	22.55329967386598	26.7936378045341	26.284972014443646	24.36809050715628
47	21.66308488621061	25.7707448646802	29.463289067558602	23.102881181550586
48	22.213659493836822	24.25388401387162	28.38478253097479	25.14767396131677
49	21.24320223089971	25.292269312814565	29.272812498758626	24.1917159575271
50	20.616357547758177	25.013406849211677	29.104382748662296	25.265852854367854
51	20.76293909913918	24.268581893007386	27.60221977698948	27.36625923086396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	513.0
2	783.0
3	1879.5
4	2976.0
5	2070.0
6	1164.0
7	1137.5
8	1111.0
9	1081.0
10	1051.0
11	1057.0
12	1063.0
13	1036.5
14	1010.0
15	1024.5
16	1039.0
17	1060.0
18	1081.0
19	1156.5
20	1232.0
21	1324.5
22	1417.0
23	1652.5
24	1888.0
25	2215.0
26	3008.0
27	3474.0
28	4458.5
29	5443.0
30	6827.0
31	8211.0
32	9056.5
33	9902.0
34	11891.0
35	13880.0
36	14541.5
37	15203.0
38	16545.5
39	17888.0
40	20125.0
41	22362.0
42	24418.5
43	26475.0
44	28951.0
45	31427.0
46	37969.5
47	44512.0
48	43576.0
49	42640.0
50	42342.5
51	42045.0
52	38275.5
53	34506.0
54	31268.0
55	28030.0
56	26809.5
57	25589.0
58	25358.5
59	25128.0
60	25036.5
61	24945.0
62	23108.5
63	21272.0
64	17686.0
65	14100.0
66	11606.0
67	9112.0
68	7792.5
69	6473.0
70	5586.5
71	4700.0
72	3931.5
73	3163.0
74	2612.0
75	1560.5
76	1060.0
77	851.5
78	643.0
79	514.0
80	385.0
81	255.0
82	125.0
83	92.0
84	59.0
85	42.5
86	26.0
87	17.5
88	9.0
89	10.5
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	5.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	503474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.47219291964779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91027007561712	23.02611969879766
2	8.638297293612105	5.610089124308872
3	3.4018065291766675	3.3139235367222653
4	2.1552620283696426	2.799443375104417
5	1.6619554391180038	2.6983668821448883
6	1.3984225084976898	2.724590728746887
7	1.2043091445064729	2.7374591212717116
8	1.061819111212633	2.758367602005245
9	0.9600028069756806	2.805605671636593
>10	8.493418199724596	45.68747025251472
>50	0.07422931666342718	1.5767959353549337
>100	0.035258925415127915	2.1593065356771386
>500	0.0018557329165856794	0.3908846993886243
>1k	0.003092888194309466	1.7115768363260369
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	2105	0.41809507541600954	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	1834	0.36426905858097935	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	1740	0.34559877967879177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.3167988813722258	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	1247	0.24767912543646742	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	781	0.155122210878814	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	630	0.12513059264232115	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	535	0.10626169375181241	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9723997664228936E-4	0.0	0.0	0.11261753337808904	0.0
2	3.9723997664228936E-4	0.0	0.0	0.5950654850101494	0.0
3	3.9723997664228936E-4	0.0	0.0	0.8286425912758156	0.0
4	3.9723997664228936E-4	0.0	0.0	1.2006578294013197	0.0
5	3.9723997664228936E-4	0.0	0.0	2.35801650134863	0.0
6	3.9723997664228936E-4	0.0	0.0	2.9520888864171733	0.0
7	3.9723997664228936E-4	0.0	0.0	3.47724013553828	0.0
8	3.9723997664228936E-4	0.0	0.0	4.242125710563008	0.0
9	3.9723997664228936E-4	0.0	0.0	4.504105475158598	0.0
10	3.9723997664228936E-4	0.0	0.0	5.4564883191584865	0.0
11	3.9723997664228936E-4	0.0	0.0	6.304992909266417	0.0
12	3.9723997664228936E-4	0.0	0.0	7.355494027496952	0.0
13	3.9723997664228936E-4	0.0	0.0	7.661766049488156	0.0
14	3.9723997664228936E-4	0.0	0.0	7.807155880939234	0.0
15	3.9723997664228936E-4	0.0	0.0	8.086216964530443	0.0
16	3.9723997664228936E-4	0.0	0.0	8.572637315928926	0.0
17	3.9723997664228936E-4	0.0	0.0	9.151813201873384	0.0
18	3.9723997664228936E-4	0.0	0.0	9.743700767070395	0.0
19	3.9723997664228936E-4	0.0	0.0	10.168747542077645	0.0
20	5.95859964963434E-4	0.0	0.0	10.526064901067384	1.9861998832114468E-4
21	5.95859964963434E-4	0.0	0.0	10.9592550955958	1.9861998832114468E-4
22	5.95859964963434E-4	0.0	0.0	11.457989886270195	1.9861998832114468E-4
23	5.95859964963434E-4	0.0	0.0	11.921966178988388	1.9861998832114468E-4
24	5.95859964963434E-4	0.0	0.0	12.287824197475937	1.9861998832114468E-4
25	5.95859964963434E-4	0.0	0.0	12.631238157283196	1.9861998832114468E-4
26	5.95859964963434E-4	0.0	0.0	12.942078439005789	1.9861998832114468E-4
27	5.95859964963434E-4	0.0	0.0	13.269205559770713	1.9861998832114468E-4
28	5.95859964963434E-4	0.0	0.0	13.599311980360456	1.9861998832114468E-4
29	5.95859964963434E-4	0.0	0.0	13.942725940167715	1.9861998832114468E-4
30	5.95859964963434E-4	0.0	0.0	14.330829397347232	1.9861998832114468E-4
31	7.944799532845787E-4	0.0	0.0	14.706817035239158	1.9861998832114468E-4
32	7.944799532845787E-4	0.0	0.0	15.067312314042036	1.9861998832114468E-4
33	7.944799532845787E-4	0.0	0.0	15.451244751466808	1.9861998832114468E-4
34	7.944799532845787E-4	0.0	0.0	15.785124951834653	1.9861998832114468E-4
35	7.944799532845787E-4	0.0	0.0	16.146414710590815	1.9861998832114468E-4
36	7.944799532845787E-4	0.0	0.0	16.519224428669602	1.9861998832114468E-4
37	7.944799532845787E-4	0.0	0.0	16.885678307122117	1.9861998832114468E-4
38	9.930999416057234E-4	0.0	0.0	17.249947365703093	1.9861998832114468E-4
39	9.930999416057234E-4	0.0	0.0	17.59792958524174	1.9861998832114468E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAG	20	7.029505E-4	45.0	13
GATCGGC	20	7.029505E-4	45.0	15
ATTACCG	20	7.029505E-4	45.0	1
TTCGTCA	20	7.029505E-4	45.0	45
TAGCCGT	25	3.8876722E-5	45.0	44
CCGTCGA	25	3.8876722E-5	45.0	41
CGGGCTT	20	7.029505E-4	45.0	6
TCCGTCG	20	7.029505E-4	45.0	40
TAATGCG	20	7.029505E-4	45.0	1
TATGGCC	110	0.0	40.909092	10
CGTTAGG	50	1.0786607E-9	40.5	2
CATACGA	100	0.0	40.5	18
CGTCGAA	50	1.0786607E-9	40.5	42
CGATGAA	390	0.0	40.384617	19
CGAATAT	135	0.0	40.0	14
GCTACGA	135	0.0	40.0	10
TCGATCA	85	0.0	39.705883	17
CCGATGA	405	0.0	39.444447	18
TACGGCT	275	0.0	39.272728	7
CCTATGC	115	0.0	39.130436	35
>>END_MODULE