##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407296
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177686498271527	31.0	31.0	33.0	28.0	34.0
2	31.178739786297925	31.0	31.0	34.0	28.0	34.0
3	31.00288733500943	31.0	31.0	34.0	27.0	34.0
4	34.84608491121936	35.0	35.0	37.0	32.0	37.0
5	34.83464114550597	35.0	35.0	37.0	32.0	37.0
6	34.985192587209305	36.0	35.0	37.0	32.0	37.0
7	35.4869259703017	37.0	35.0	37.0	33.0	37.0
8	35.233297159805154	37.0	35.0	37.0	32.0	37.0
9	37.13486506128221	39.0	37.0	39.0	34.0	39.0
10	36.39992536140792	38.0	35.0	39.0	32.0	39.0
11	36.55316526555625	38.0	35.0	39.0	32.0	39.0
12	36.60913929918291	39.0	35.0	39.0	33.0	39.0
13	36.63075011785041	39.0	35.0	39.0	33.0	39.0
14	37.574091078724074	39.0	36.0	41.0	33.0	41.0
15	37.81011843966059	40.0	37.0	41.0	33.0	41.0
16	37.82233314346323	39.0	36.0	41.0	33.0	41.0
17	37.73833526477058	39.0	36.0	41.0	33.0	41.0
18	37.70702388434947	39.0	36.0	41.0	33.0	41.0
19	37.58992231693903	39.0	36.0	41.0	33.0	41.0
20	37.63159225722816	39.0	36.0	41.0	33.0	41.0
21	37.55254655091137	39.0	36.0	41.0	33.0	41.0
22	37.68647126414205	39.0	36.0	41.0	33.0	41.0
23	37.74231762649277	40.0	36.0	41.0	33.0	41.0
24	37.71694050518542	40.0	35.0	41.0	33.0	41.0
25	37.30439532919547	39.0	35.0	41.0	32.0	41.0
26	37.3902027027027	39.0	35.0	41.0	33.0	41.0
27	37.44395967551854	39.0	35.0	41.0	33.0	41.0
28	37.31603060182275	39.0	35.0	41.0	32.0	41.0
29	37.29905032212445	39.0	35.0	41.0	32.0	41.0
30	37.00756452309868	39.0	35.0	41.0	31.0	41.0
31	37.00607666169076	39.0	35.0	41.0	32.0	41.0
32	37.00252641813325	39.0	35.0	41.0	32.0	41.0
33	37.00101645977373	39.0	35.0	41.0	32.0	41.0
34	36.90351734365179	39.0	35.0	41.0	31.0	41.0
35	36.88197281583909	39.0	35.0	41.0	31.0	41.0
36	36.72953822281584	39.0	35.0	41.0	31.0	41.0
37	36.7288777694846	39.0	35.0	41.0	31.0	41.0
38	36.56911435417976	39.0	35.0	41.0	31.0	41.0
39	36.47975919233187	39.0	35.0	41.0	31.0	41.0
40	36.361655896448774	39.0	35.0	41.0	30.0	41.0
41	36.14196063796354	39.0	35.0	40.0	30.0	41.0
42	36.19174752514142	39.0	35.0	41.0	30.0	41.0
43	36.17133976272785	39.0	35.0	40.0	30.0	41.0
44	36.184489904148336	38.0	35.0	40.0	30.0	41.0
45	36.16166129792583	38.0	35.0	40.0	30.0	41.0
46	36.05686773255814	38.0	35.0	40.0	30.0	41.0
47	36.0087233854494	38.0	35.0	40.0	30.0	41.0
48	35.90227009349466	38.0	35.0	40.0	30.0	41.0
49	35.8882876335638	38.0	35.0	40.0	30.0	41.0
50	35.76820788812068	38.0	35.0	40.0	29.0	41.0
51	34.60536317567568	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	31.0
10	40.0
11	21.0
12	15.0
13	19.0
14	28.0
15	40.0
16	60.0
17	112.0
18	182.0
19	294.0
20	506.0
21	844.0
22	1238.0
23	1701.0
24	2309.0
25	3042.0
26	3626.0
27	4374.0
28	4916.0
29	5697.0
30	7094.0
31	9046.0
32	12448.0
33	17858.0
34	31213.0
35	31318.0
36	34175.0
37	51549.0
38	85875.0
39	97590.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.545725958516655	20.733324167190446	23.25532291011942	12.465626964173476
2	33.77813678504085	24.891970458830922	23.467944688874923	17.8619480672533
3	33.52696810182275	24.240355908233816	25.739756835323696	16.492919154619738
4	29.593219673161535	24.496680546825896	25.873320631678187	20.03677914833438
5	26.32581709616593	29.505322910119418	24.03608186675047	20.132778126964173
6	25.947222658705215	34.57338152105594	24.45371425204274	15.025681568196102
7	79.0525809239472	6.004478315524827	10.192096165933377	4.750844594594595
8	79.84635252985544	6.483245600251414	8.897460323695789	4.7729415461973606
9	74.50822006599623	7.419174261470774	10.351194217473287	7.721411455059711
10	43.936350958516655	24.650131599622878	16.85334498742929	14.560172454431175
11	32.71208163104966	25.29339841294783	23.29755263984915	18.696967316153362
12	29.594938324952857	21.849220223130107	28.082524748585797	20.47331670333124
13	27.029727765556256	22.439454352608422	29.772204981143936	20.75861290069139
14	20.656721401634194	27.317724701445634	29.54264220615965	22.482911690760528
15	19.989884506599623	25.45568824638592	32.97650848522942	21.577918761785043
16	24.66780916090509	24.412712130735386	30.06192056882464	20.857558139534884
17	24.34494814582024	24.682049418604652	27.45497132306725	23.518031112507856
18	25.048367771841605	25.242329902576994	27.762118950345695	21.947183375235703
19	25.823725251414203	26.275239629164048	26.020633642363293	21.880401477058452
20	27.382787947831556	26.011303818353237	26.848532762413573	19.757375471401634
21	26.587297690131994	26.291935103708358	28.08375235700817	19.037014849151475
22	24.351577231301068	23.614275612822127	28.584862115022	23.449285040854807
23	24.234708909490887	25.743439660590823	28.504331002514142	21.51752042740415
24	23.906691939032054	25.33268188246386	27.898383485229417	22.86224269327467
25	24.033381128221247	26.706866750471402	26.310594751728473	22.94915736957888
26	22.94915736957888	26.507012099308614	26.566673868636077	23.97715666247643
27	24.120786847894408	25.56887374292898	27.963937774984288	22.346401634192333
28	21.707554211187933	27.061154541169074	28.263474230044	22.967817017598993
29	23.87551068510371	25.9938717787555	27.700738529226903	22.429879006913893
30	24.48735072281584	24.66584498742929	28.84609718730358	22.00070710245129
31	25.35134153048397	24.763808139534884	27.04863293526084	22.8362173947203
32	25.756943353236956	25.483432196731613	26.494736015084854	22.264888434946574
33	24.97913065681961	24.535227451288495	27.82178071967316	22.66386117221873
34	24.47261942174733	24.441438167818983	28.645997014456313	22.43994539597737
35	24.13429054054054	24.2494402105594	27.487380185417976	24.128889063482088
36	24.945248664362037	26.26738293526084	27.253889063482085	21.533479336895034
37	23.463770820238842	26.435074245757384	27.767274905719674	22.3338800282841
38	23.05497721558768	25.54432157448146	27.221971244500313	24.17872996543055
39	23.902763592080454	24.073155641106222	27.05133367379007	24.972747093023255
40	25.217286690760528	22.734325895663105	29.435594751728473	22.612792661847894
41	22.398452231301068	24.247476037083597	28.544351037083594	24.80972069453174
42	22.588731536769327	24.470655248271527	28.95191703331238	23.988696181646763
43	23.160060496543057	23.940573931489627	28.95780955373979	23.94155601822753
44	23.179947752985544	24.183149355751098	28.653853708359524	23.983049182903834
45	23.31694885292269	23.051294390320553	27.115414833438088	26.516341923318663
46	23.551667583280956	24.98477765556254	27.082269406033944	24.381285355122564
47	22.603217316153362	24.671246464487744	30.095065996228787	22.630470223130107
48	22.260223522941548	24.73753731929604	28.736594516027658	24.265644641734756
49	23.211129006913893	23.994834223758644	29.197438717787556	23.596598051539914
50	21.543545725958516	24.19542543997486	29.435349230044	24.82567960402263
51	21.203252671275926	23.745875235700815	28.07344044626021	26.977431646763044
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	406.5
2	646.0
3	1685.5
4	2725.0
5	1832.0
6	939.0
7	927.5
8	916.0
9	899.5
10	883.0
11	875.0
12	867.0
13	898.5
14	930.0
15	910.0
16	890.0
17	862.0
18	834.0
19	896.0
20	958.0
21	1061.0
22	1164.0
23	1345.0
24	1526.0
25	1721.0
26	2327.0
27	2738.0
28	3426.0
29	4114.0
30	5035.5
31	5957.0
32	6576.5
33	7196.0
34	8332.0
35	9468.0
36	10309.0
37	11150.0
38	11929.5
39	12709.0
40	14364.5
41	16020.0
42	17962.5
43	19905.0
44	21793.5
45	23682.0
46	29007.0
47	34332.0
48	34071.5
49	33811.0
50	34827.5
51	35844.0
52	32069.5
53	28295.0
54	26476.0
55	24657.0
56	24383.0
57	24109.0
58	23536.5
59	22964.0
60	21708.5
61	20453.0
62	18880.5
63	17308.0
64	14675.0
65	12042.0
66	10170.5
67	8299.0
68	7253.5
69	6208.0
70	5212.5
71	4217.0
72	3470.0
73	2723.0
74	2226.5
75	1342.5
76	955.0
77	745.5
78	536.0
79	415.0
80	294.0
81	214.5
82	135.0
83	91.0
84	47.0
85	29.5
86	12.0
87	9.5
88	7.0
89	8.0
90	9.0
91	6.0
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.160757947988195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69092317738945	24.855464389600193
2	8.42936696662279	5.92765863136383
3	3.603248324512974	3.8007882649408415
4	2.323016811487236	3.2671612727124018
5	1.878626950207278	3.302697373540269
6	1.5174022091994128	3.2011807072441862
7	1.4143767127729725	3.48113900715572
8	1.2233275645512667	3.4410499510631194
9	1.181284742173116	3.738138019848053
>10	7.655226866554829	40.137295703543764
>50	0.05429131303987287	1.2933577959534883
>100	0.02397278757604776	1.6399390109703518
>500	0.0021152459625924497	0.5057408287799724
>1k	0.0028203279501232657	1.408389043283835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	1670	0.41002121307353867	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1565	0.38424143620364554	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	1267	0.311075974230044	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	1179	0.2894700659962288	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	899	0.22072399434318038	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	618	0.15173240100565683	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	523	0.1284078409805154	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	428	0.10508328095537398	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09845419547454431	0.0
2	0.0	0.0	0.0	0.5632267441860465	0.0
3	0.0	0.0	0.0	0.7969633878064111	0.0
4	0.0	0.0	0.0	1.1429034412319297	0.0
5	0.0	0.0	0.0	2.3263179604022626	0.0
6	0.0	0.0	0.0	2.9963466373350096	0.0
7	0.0	0.0	0.0	3.5632562067881834	0.0
8	0.0	0.0	0.0	4.312342866121936	0.0
9	0.0	0.0	0.0	4.601321888749214	0.0
10	0.0	0.0	0.0	5.484463387806411	0.0
11	0.0	0.0	0.0	6.359748192960402	0.0
12	0.0	0.0	0.0	7.362213230672533	0.0
13	0.0	0.0	0.0	7.682373507228158	0.0
14	0.0	0.0	0.0	7.832141734758014	0.0
15	0.0	0.0	0.0	8.077172375864237	0.0
16	0.0	0.0	0.0	8.504871150219987	0.0
17	0.0	0.0	0.0	8.99247721558768	0.0
18	0.0	0.0	0.0	9.555212916404777	0.0
19	0.0	0.0	0.0	9.932579745443118	0.0
20	0.0	0.0	0.0	10.282202624135763	0.0
21	0.0	0.0	0.0	10.683630578252671	0.0
22	0.0	0.0	0.0	11.140791954745444	0.0
23	0.0	0.0	0.0	11.571436989314897	0.0
24	0.0	0.0	0.0	11.902645741671904	0.0
25	0.0	0.0	0.0	12.221578409805154	0.0
26	0.0	0.0	0.0	12.527743950345695	0.0
27	0.0	0.0	0.0	12.866072831552483	0.0
28	0.0	0.0	0.0	13.188442803268385	0.0
29	0.0	0.0	0.0	13.52898137963545	0.0
30	0.0	0.0	0.0	13.961590587680703	0.0
31	0.0	0.0	0.0	14.299919468887492	0.0
32	2.4552168447517284E-4	0.0	0.0	14.648805782526713	0.0
33	2.4552168447517284E-4	0.0	0.0	14.980505578252671	0.0
34	2.4552168447517284E-4	0.0	0.0	15.318588937774985	0.0
35	2.4552168447517284E-4	0.0	0.0	15.701111722187303	0.0
36	2.4552168447517284E-4	0.0	0.0	16.052453252671278	0.0
37	2.4552168447517284E-4	0.0	0.0	16.43571260213702	0.0
38	2.4552168447517284E-4	0.0	0.0	16.82682864550597	0.0
39	2.4552168447517284E-4	0.0	0.0	17.42000903519799	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.027915E-4	45.000004	1
CTCACGA	40	6.7957444E-9	45.000004	24
ACTTCGT	20	7.027915E-4	45.000004	30
CACGACG	35	1.2093369E-7	45.0	26
CGAATGG	75	0.0	42.0	2
CGATGAA	190	0.0	40.263157	19
TCACGAC	45	1.9230356E-8	40.0	25
TCTAGCG	40	3.4508957E-7	39.375004	1
TACGAAT	40	3.4508957E-7	39.375004	12
TACGGGT	35	6.2383588E-6	38.571426	4
TCTCGTA	35	6.2383588E-6	38.571426	28
ATGGGAC	165	0.0	38.181816	5
AACGAAT	65	9.094947E-12	38.07692	24
AAACGGG	95	0.0	37.894737	3
GTAGGGT	125	0.0	37.8	4
GTCGAGG	30	1.1386278E-4	37.500004	2
CGGCCTA	30	1.1386278E-4	37.500004	44
TAGCACG	30	1.1386278E-4	37.500004	1
TATACGG	30	1.1386278E-4	37.500004	2
AACACGT	30	1.1386278E-4	37.500004	41
>>END_MODULE