##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935559.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372857
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172672096809233	31.0	31.0	33.0	28.0	34.0
2	31.180398919693072	31.0	31.0	34.0	28.0	34.0
3	30.980335624649666	31.0	31.0	34.0	27.0	34.0
4	34.82149993160917	35.0	35.0	37.0	32.0	37.0
5	34.834250128065186	35.0	35.0	37.0	33.0	37.0
6	35.02730269245314	36.0	35.0	37.0	32.0	37.0
7	35.52074924166637	37.0	35.0	37.0	33.0	37.0
8	35.258715807937094	37.0	35.0	37.0	32.0	37.0
9	37.14393721989932	39.0	37.0	39.0	34.0	39.0
10	36.315995140228026	38.0	35.0	39.0	32.0	39.0
11	36.59708681880721	38.0	35.0	39.0	32.0	39.0
12	36.71956809178854	39.0	35.0	39.0	33.0	39.0
13	36.75478534666105	39.0	35.0	39.0	33.0	39.0
14	37.788822524453074	40.0	37.0	41.0	33.0	41.0
15	38.005476630450815	40.0	37.0	41.0	33.0	41.0
16	37.94183292790534	39.0	37.0	41.0	33.0	41.0
17	37.856438259171746	39.0	36.0	41.0	33.0	41.0
18	37.871049759022895	39.0	37.0	41.0	33.0	41.0
19	37.73873898035976	39.0	36.0	41.0	33.0	41.0
20	37.84996392718924	39.0	36.0	41.0	33.0	41.0
21	37.77541792161606	39.0	36.0	41.0	33.0	41.0
22	37.8890271605468	40.0	36.0	41.0	33.0	41.0
23	37.91191529192157	40.0	36.0	41.0	34.0	41.0
24	37.898617968819146	40.0	36.0	41.0	33.0	41.0
25	37.44393963369335	39.0	35.0	41.0	33.0	41.0
26	37.56784236315799	39.0	36.0	41.0	33.0	41.0
27	37.62038261317341	39.0	36.0	41.0	33.0	41.0
28	37.559528183727274	40.0	36.0	41.0	33.0	41.0
29	37.54714273836886	40.0	36.0	41.0	33.0	41.0
30	37.25304339197065	39.0	35.0	41.0	32.0	41.0
31	37.26353266802018	39.0	35.0	41.0	32.0	41.0
32	37.26092040648292	39.0	35.0	41.0	33.0	41.0
33	37.25868898800344	39.0	35.0	41.0	33.0	41.0
34	37.18899470842709	39.0	35.0	41.0	32.0	41.0
35	37.21704835902236	40.0	35.0	41.0	32.0	41.0
36	37.0562011709583	39.0	35.0	41.0	32.0	41.0
37	37.069774739377294	39.0	35.0	41.0	32.0	41.0
38	36.89767122516139	39.0	35.0	41.0	31.0	41.0
39	36.80506467626999	39.0	35.0	41.0	31.0	41.0
40	36.70966617228589	39.0	35.0	41.0	31.0	41.0
41	36.55317185945282	39.0	35.0	41.0	31.0	41.0
42	36.58775616389125	39.0	35.0	41.0	31.0	41.0
43	36.58070520333533	39.0	35.0	41.0	31.0	41.0
44	36.56465079105394	39.0	35.0	41.0	31.0	41.0
45	36.53722204491266	39.0	35.0	41.0	31.0	41.0
46	36.4061074352902	39.0	35.0	40.0	31.0	41.0
47	36.37562121671311	39.0	35.0	40.0	31.0	41.0
48	36.30108862110675	38.0	35.0	40.0	31.0	41.0
49	36.27639550819751	38.0	35.0	40.0	31.0	41.0
50	36.17119163647189	38.0	35.0	40.0	31.0	41.0
51	34.981867042860934	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	22.0
10	34.0
11	15.0
12	16.0
13	17.0
14	27.0
15	31.0
16	49.0
17	85.0
18	141.0
19	209.0
20	423.0
21	630.0
22	930.0
23	1312.0
24	1715.0
25	2335.0
26	2994.0
27	3420.0
28	3920.0
29	4606.0
30	5796.0
31	7575.0
32	10023.0
33	14920.0
34	26704.0
35	29682.0
36	31555.0
37	47876.0
38	82674.0
39	93097.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39702888775053	20.361961824506448	23.062192744135153	11.178816543607871
2	34.143652928602656	23.842921012613417	24.370737306795903	17.64268875198803
3	33.189936088098115	23.927672002939467	26.08319007018777	16.799201838774653
4	29.448018945601127	25.609550041973193	25.285028844838635	19.657402167587037
5	24.883534438135797	30.227406217397018	24.517710543184116	20.371348801283066
6	26.08453106687014	35.591392946893855	23.799740919440964	14.524335066795043
7	80.00520306712761	6.036898864712209	9.268969068570525	4.688928999589655
8	80.45524155373239	6.255213124602729	8.308010845981167	4.9815344756837066
9	74.46876416427746	7.533182962905349	10.780808728279206	7.217244144537986
10	40.730360433088286	24.77598650420939	18.980467042324538	15.513186020377786
11	31.458441171816542	26.051006149810785	23.493993675859645	18.996559002513028
12	28.3993595399845	22.50380172559453	29.066103090460953	20.030735643960018
13	26.243036874726773	24.601120536827793	29.833153192779	19.322689395666433
14	21.685793749346264	28.13089200417318	28.260432283690527	21.922881962790026
15	20.559624735488406	26.479052290824633	32.19572114778588	20.765601825901083
16	23.42050705766554	24.39004765902209	30.88181259839563	21.30763268491674
17	23.514108626095258	25.128132233000855	27.55479982942522	23.802959311478663
18	24.313879047463235	24.939588099459044	28.790394172564817	21.956138680512904
19	25.105067090064022	26.902539043118406	26.293190150647565	21.699203716170008
20	26.2811211805062	25.93273024242538	27.350163735694917	20.435984841373504
21	25.196254864465466	27.002577395623522	27.781696468082938	20.019471271828074
22	23.745564653473046	25.10238509669927	27.407558393700533	23.744491856127148
23	23.05039197333026	26.917021807288048	27.084378193248348	22.948208026133344
24	23.77506658048528	24.984645587986815	28.551428563765736	22.688859267762172
25	22.24177097385861	26.683151985881988	27.451006686209457	23.624070354049945
26	21.65521902498813	27.182539150398142	28.163075924550164	22.999165900063563
27	22.275027691581492	27.12487629305605	28.216447592508658	22.383648422853803
28	21.13330311620809	26.90656203316553	28.856103010001156	23.10403184062523
29	22.269663704851993	25.753304886323715	28.37763539373004	23.59939601509426
30	23.1501621264989	26.466983320683262	27.53951246724616	22.84334208557168
31	23.64874469300563	27.127290087084326	24.96613983377005	24.257825386139995
32	24.943611089506167	27.42794154327262	25.06644638561164	22.56200098160957
33	24.09905137894689	27.03556591400993	26.15989508041957	22.70548762662361
34	22.776560450789447	25.905910308777898	27.357136918443263	23.960392321989396
35	22.984414936557446	26.08319007018777	26.73303706246631	24.19935793078848
36	22.48368677535892	28.57288451068372	26.149435306297054	22.79399340766031
37	23.33361047264769	28.562424736561205	26.337979439838865	21.765985350952242
38	22.874185009266288	27.75299913908013	25.063227993573946	24.309587858079638
39	24.277135738366184	25.648170746425574	26.981389648042008	23.09330386716623
40	23.84479840796874	25.075565163051788	28.40445532737752	22.675181101601954
41	21.17648320938054	27.375106273987075	27.060776651638562	24.38763386499382
42	21.88989344440362	27.94744365802439	26.33824763917534	23.82441525839665
43	22.776828650125918	26.40744306798585	26.938477754206037	23.877250527682197
44	22.064759411785214	25.97591033559783	27.052998870880796	24.90633138173616
45	22.802843985763978	25.1383238077869	26.384646124385487	25.67418608206363
46	23.525641197563676	26.830929820279625	26.46242393196319	23.181005050193505
47	21.82847579635088	26.937941355533084	28.031121851004542	23.202460997111494
48	22.09828432884457	25.70100601571112	27.733420587517465	24.467289067926846
49	22.418246137259057	24.528170317306635	29.297559117838745	23.756024427595566
50	21.377900911073148	24.82050759406421	28.975719914068932	24.82587158079371
51	20.93939499593678	25.23407097090842	26.533496756129026	27.29303727702578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	343.0
2	577.0
3	1029.0
4	1481.0
5	1089.5
6	698.0
7	690.0
8	682.0
9	666.5
10	651.0
11	648.5
12	646.0
13	707.0
14	768.0
15	762.5
16	757.0
17	798.5
18	840.0
19	799.5
20	759.0
21	986.0
22	1213.0
23	1330.5
24	1448.0
25	1646.0
26	2254.5
27	2665.0
28	3131.0
29	3597.0
30	4488.0
31	5379.0
32	6023.5
33	6668.0
34	7593.5
35	8519.0
36	9675.5
37	10832.0
38	11781.5
39	12731.0
40	14958.5
41	17186.0
42	18676.0
43	20166.0
44	22337.0
45	24508.0
46	28989.5
47	33471.0
48	34346.5
49	35222.0
50	35012.0
51	34802.0
52	31683.0
53	28564.0
54	25743.5
55	22923.0
56	21359.5
57	19796.0
58	18533.0
59	17270.0
60	16815.0
61	16360.0
62	14347.5
63	12335.0
64	10708.5
65	9082.0
66	7640.5
67	6199.0
68	5297.5
69	4396.0
70	3668.0
71	2940.0
72	2515.0
73	2090.0
74	1675.0
75	1014.5
76	769.0
77	537.5
78	306.0
79	250.5
80	195.0
81	144.5
82	94.0
83	63.5
84	33.0
85	22.5
86	12.0
87	11.0
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	372857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.73507133627438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90348477395389	26.781147138341893
2	8.798511381724436	6.464278865213384
3	3.387175063046514	3.732843527083901
4	2.055171482437774	3.019874840625136
5	1.4953379391592925	2.7465672933427068
6	1.281426229618839	2.82439703743327
7	1.1167644840033086	2.8717096089975436
8	0.9576495005685498	2.814345817482657
9	0.9107255150060084	3.011001008535985
>10	7.011166531955817	41.43356383436409
>50	0.05116014952912974	1.2371635699378036
>100	0.026310934043552437	1.5421587555821927
>500	0.004385155673925406	1.203066396894813
>1k	7.308592789875678E-4	0.3178823061645745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1184	0.3175480143862124	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC	929	0.2491571835851278	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	827	0.22180085126469398	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	762	0.20436789439382927	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	732	0.19632191429958404	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	720	0.19310352226188593	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	511	0.13704986093864405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.681993364748416E-4	0.0	0.0	0.14482764169641443	0.0
2	2.681993364748416E-4	0.0	0.0	0.8298087470531598	0.0
3	2.681993364748416E-4	0.0	0.0	1.1057858642857719	0.0
4	2.681993364748416E-4	0.0	0.0	1.5043300782873863	0.0
5	2.681993364748416E-4	0.0	0.0	2.720345869864318	0.0
6	2.681993364748416E-4	0.0	0.0	3.4061315732304878	0.0
7	2.681993364748416E-4	0.0	0.0	3.9773961599219003	0.0
8	2.681993364748416E-4	0.0	0.0	4.881227923842117	0.0
9	2.681993364748416E-4	0.0	0.0	5.165787419841923	0.0
10	2.681993364748416E-4	0.0	0.0	6.111994678925164	0.0
11	2.681993364748416E-4	0.0	0.0	7.326133075146772	0.0
12	2.681993364748416E-4	0.0	0.0	8.42843234805837	0.0
13	2.681993364748416E-4	0.0	0.0	8.855137492389844	0.0
14	2.681993364748416E-4	0.0	0.0	9.042608828585758	0.0
15	2.681993364748416E-4	0.0	0.0	9.388049573965354	0.0
16	2.681993364748416E-4	0.0	0.0	10.058547915152458	0.0
17	2.681993364748416E-4	0.0	0.0	10.828011811498778	0.0
18	2.681993364748416E-4	0.0	0.0	11.671498724712155	0.0
19	2.681993364748416E-4	0.0	0.0	12.175981676621332	0.0
20	2.681993364748416E-4	0.0	0.0	12.675368841137487	0.0
21	2.681993364748416E-4	0.0	0.0	13.238051049061704	0.0
22	2.681993364748416E-4	0.0	0.0	13.888970838686145	0.0
23	2.681993364748416E-4	0.0	0.0	14.52299407011267	0.0
24	2.681993364748416E-4	0.0	0.0	15.009507666478033	0.0
25	2.681993364748416E-4	0.0	0.0	15.475638113271307	0.0
26	2.681993364748416E-4	0.0	0.0	15.903147855612206	0.0
27	2.681993364748416E-4	0.0	0.0	16.32609820923303	0.0
28	2.681993364748416E-4	0.0	0.0	16.775600297164864	0.0
29	2.681993364748416E-4	0.0	0.0	17.216520006329503	0.0
30	2.681993364748416E-4	0.0	0.0	17.688550838525227	0.0
31	2.681993364748416E-4	0.0	0.0	18.158704275365622	0.0
32	2.681993364748416E-4	0.0	0.0	18.61169295467163	0.0
33	2.681993364748416E-4	0.0	0.0	19.076750604119006	0.0
34	5.363986729496832E-4	0.0	0.0	19.5128427252271	0.0
35	5.363986729496832E-4	0.0	0.0	19.993455936190013	0.0
36	8.045980094245246E-4	0.0	0.0	20.444299020804223	0.0
37	0.0010727973458993663	0.0	0.0	20.863226384377924	0.0
38	0.0010727973458993663	0.0	0.0	21.309510080272062	0.0
39	0.0013409966823742078	0.0	0.0	21.768935543653466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0271484E-4	45.000004	6
CGATTGT	20	7.0271484E-4	45.000004	10
TATGCGG	40	6.7939254E-9	45.000004	2
CGTTAGG	60	0.0	45.000004	2
ATGCGCG	30	2.1615415E-6	45.000004	1
TGCGTAG	40	6.7939254E-9	45.000004	1
GCGAAAG	25	3.8857186E-5	45.0	1
GCGTTAG	50	2.1827873E-11	45.0	1
ACGGGCG	70	0.0	41.785717	5
CGAATAT	45	1.922308E-8	40.0	14
GCTACGA	45	1.922308E-8	40.0	10
GGCGATA	130	0.0	39.80769	8
AAATCGA	40	3.4498407E-7	39.375004	15
CGTTGAT	40	3.4498407E-7	39.375004	25
CACGAGG	40	3.4498407E-7	39.375004	2
CGCAAGG	80	0.0	39.375004	2
TAATCGT	40	3.4498407E-7	39.375004	21
TCGTCTG	75	0.0	39.0	18
ATGGGCG	105	0.0	38.57143	5
TTTCGTC	70	0.0	38.57143	16
>>END_MODULE