SRR2935557.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935557.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	3166	1.243734188155062	Illumina PCR Primer Index 9 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2637	1.035921368971857	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	1876	0.7369694684077375	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT	1867	0.7334339005955468	Illumina PCR Primer Index 9 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	1730	0.6796147016766448	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	768	0.30170178664026776	Illumina PCR Primer Index 9 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTG	397	0.15595782460440924	Illumina PCR Primer Index 9 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGAACT	394	0.1547793020003457	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCT	356	0.13985134901554078	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT	321	0.12610191863479941	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTC	311	0.12217350995458759	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT	309	0.12138782821854524	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTA	297	0.11667373780229104	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	268	0.10528135262967678	Illumina PCR Primer Index 9 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	30	2.158822E-6	45.000004	13
CACGGGA	30	2.158822E-6	45.000004	4
CCGATTA	25	3.882224E-5	45.0	18
AGGCGTC	20	7.0229307E-4	45.0	35
ACGGGCC	40	6.7793735E-9	45.0	5
TATGACG	20	7.0229307E-4	45.0	1
TCGTCCT	20	7.0229307E-4	45.0	24
GTCCGGC	20	7.0229307E-4	45.0	33
ACATACG	25	3.882224E-5	45.0	17
GCGTCAT	20	7.0229307E-4	45.0	37
CGTCATA	20	7.0229307E-4	45.0	38
TCGAATA	20	7.0229307E-4	45.0	44
CGTTTTT	1215	0.0	43.333332	1
GATGAAT	480	0.0	42.656254	20
CGATGAA	475	0.0	42.157894	19
CCGATGA	470	0.0	42.12766	18
CTCGAAT	75	0.0	42.000004	43
CACCGAT	520	0.0	41.105766	16
CGTAAGG	55	6.002665E-11	40.909092	2
CCACCGA	565	0.0	40.61947	15