SRR2935555.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357819
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1260	0.3521333411585187	Illumina PCR Primer Index 9 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	1139	0.31831736157107365	RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.27527884209614356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	887	0.24789069333936992	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	835	0.233358206243939	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	765	0.21379524284624352	RNA PCR Primer, Index 17 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	665	0.1858481522781071	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	561	0.15678317808724523	RNA PCR Primer, Index 17 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	381	0.10647841506459971	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGT	25	3.8854036E-5	45.0	4
TAAGTCG	20	7.0267665E-4	45.0	1
CCAACGG	35	1.2087912E-7	45.0	2
ATACGAG	20	7.0267665E-4	45.0	1
GCGTACG	20	7.0267665E-4	45.0	1
GATAGCG	20	7.0267665E-4	45.0	1
AGCGACT	30	2.161296E-6	44.999996	19
CGTTTTT	650	0.0	40.84615	1
CGTGCGG	45	1.9219442E-8	40.000004	2
GGCGTAG	45	1.9219442E-8	40.000004	1
TAGCGCG	40	3.4493314E-7	39.375	1
TAACGGG	80	0.0	39.375	3
CGACAGG	35	6.236234E-6	38.571426	2
CTACGAA	70	0.0	38.571426	11
TACGAAT	70	0.0	38.571426	12
GCTACGA	70	0.0	38.571426	10
TCCAACG	30	1.1383512E-4	37.499996	1
ACGTAGG	30	1.1383512E-4	37.499996	2
AACGGGA	115	0.0	37.173916	4
CACGCCG	85	0.0	37.058826	26