##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357819
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138380018948126	31.0	31.0	33.0	28.0	34.0
2	31.14754386994542	31.0	31.0	34.0	28.0	34.0
3	30.924445040649044	31.0	30.0	34.0	27.0	34.0
4	34.78026320569897	35.0	35.0	37.0	32.0	37.0
5	34.79758201772405	35.0	35.0	37.0	32.0	37.0
6	34.98068856041742	36.0	35.0	37.0	32.0	37.0
7	35.46866991411859	37.0	35.0	37.0	33.0	37.0
8	35.27095542718525	37.0	35.0	37.0	33.0	37.0
9	37.17909613519684	39.0	37.0	39.0	34.0	39.0
10	36.37779435971818	38.0	35.0	39.0	32.0	39.0
11	36.57836224459852	38.0	35.0	39.0	32.0	39.0
12	36.63470357918389	39.0	35.0	39.0	33.0	39.0
13	36.656186507703616	39.0	35.0	39.0	33.0	39.0
14	37.51134791612519	39.0	36.0	41.0	32.0	41.0
15	37.7698752721348	40.0	37.0	41.0	33.0	41.0
16	37.772295490177996	39.0	36.0	41.0	33.0	41.0
17	37.726632738898715	39.0	36.0	41.0	33.0	41.0
18	37.70886118400644	39.0	36.0	41.0	33.0	41.0
19	37.60256442503053	39.0	36.0	41.0	33.0	41.0
20	37.69279999105693	39.0	36.0	41.0	33.0	41.0
21	37.60575877748247	39.0	36.0	41.0	33.0	41.0
22	37.775355696595206	39.0	36.0	41.0	33.0	41.0
23	37.84729989184476	40.0	36.0	41.0	34.0	41.0
24	37.846981295012284	40.0	36.0	41.0	33.0	41.0
25	37.38786090174082	39.0	35.0	41.0	33.0	41.0
26	37.504439395336746	39.0	35.0	41.0	33.0	41.0
27	37.56328758394607	39.0	35.0	41.0	33.0	41.0
28	37.45703274560602	39.0	35.0	41.0	33.0	41.0
29	37.389761303899455	39.0	35.0	41.0	33.0	41.0
30	37.13716432050841	39.0	35.0	41.0	32.0	41.0
31	37.08691824637596	39.0	35.0	41.0	32.0	41.0
32	37.09469592168107	39.0	35.0	41.0	32.0	41.0
33	37.12348142496625	39.0	35.0	41.0	32.0	41.0
34	36.99950254178789	39.0	35.0	41.0	31.0	41.0
35	37.004790131323375	39.0	35.0	41.0	31.0	41.0
36	36.890701723497074	39.0	35.0	41.0	31.0	41.0
37	36.88090067883483	39.0	35.0	41.0	31.0	41.0
38	36.68987952009256	39.0	35.0	41.0	31.0	41.0
39	36.58866354218194	39.0	35.0	41.0	31.0	41.0
40	36.496337533781045	39.0	35.0	41.0	30.0	41.0
41	36.301605001411325	39.0	35.0	41.0	30.0	41.0
42	36.397552952749855	39.0	35.0	41.0	30.0	41.0
43	36.36070191912671	39.0	35.0	41.0	30.0	41.0
44	36.35552611795349	39.0	35.0	41.0	30.0	41.0
45	36.33902056626395	39.0	35.0	40.0	31.0	41.0
46	36.239939746072736	38.0	35.0	40.0	30.0	41.0
47	36.1955234350328	38.0	35.0	40.0	30.0	41.0
48	36.10439076739916	38.0	35.0	40.0	30.0	41.0
49	36.042753459151136	38.0	35.0	40.0	30.0	41.0
50	35.85690530687303	38.0	35.0	40.0	30.0	41.0
51	34.61586165072285	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	21.0
10	20.0
11	19.0
12	20.0
13	10.0
14	16.0
15	27.0
16	45.0
17	59.0
18	106.0
19	212.0
20	392.0
21	641.0
22	925.0
23	1401.0
24	1832.0
25	2542.0
26	3233.0
27	3602.0
28	4149.0
29	4951.0
30	6178.0
31	7938.0
32	10802.0
33	15690.0
34	26746.0
35	26621.0
36	29643.0
37	45972.0
38	77996.0
39	85979.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.516361624173115	20.605948817698334	23.33470274077117	11.542986817357379
2	33.8196127092189	23.60327428113096	24.80276340831538	17.77434960133475
3	32.96415226692825	24.197708897515223	26.58131625207158	16.256822583484944
4	29.360374938167062	25.265287757218037	26.154284708190456	19.22005259642445
5	25.119403944452362	30.5475673455015	24.820929017184664	19.512099692861476
6	25.830377928505754	34.22847864423074	24.939424681193564	15.001718746069939
7	79.6019775361286	5.944066692936932	9.712452385144445	4.741503385790023
8	80.79475936157667	5.679128274350999	8.608262836797376	4.917849527274963
9	75.23692146029137	7.209231482956467	10.70122044944511	6.852626607307047
10	41.01598853051403	27.5452114057666	17.687713620573533	13.751086443145836
11	30.494188402516354	25.571028928033446	25.521003635916482	18.413779033533714
12	27.341197644619207	22.141361973511746	30.70742470355124	19.810015678317807
13	26.43599138111727	23.557720523504898	31.69116229154964	18.31512580382819
14	20.727518661669727	27.60417976686537	31.450258370852303	20.218043200612602
15	20.04812488995833	25.413966279040523	35.07387813391687	19.46403069708428
16	24.779008381332464	25.3857397175667	29.446451977116922	20.388799923983914
17	25.464271042063164	24.222022866309505	27.130756052641143	23.182950038986192
18	25.16495770207843	24.18429429404252	30.363954960468842	20.286793043410213
19	24.990288386027572	25.7831473454456	27.687741567664098	21.538822700862724
20	24.797453461107434	27.910479879492144	28.019194061802192	19.272872597598226
21	24.078374820789282	26.97704705451639	30.26977326525422	18.67480485944011
22	22.748652251557353	24.102129847772197	30.330138980881394	22.819078919789053
23	21.73165762578287	25.329286594619067	30.500057291535665	22.4389984880624
24	22.61981616403824	25.568513689882316	29.71027251208013	22.101397633999312
25	23.017503262822824	26.536321436256877	27.64134939732099	22.804825903599305
26	21.70147476796928	25.76833538744449	28.36825322299822	24.16193662158801
27	22.398754677644284	25.085308493959236	29.695460554079016	22.82047627431746
28	20.70963252370612	26.433196672060454	29.211416945438895	23.64575385879453
29	22.94232558919454	26.216327249251712	28.349249201411887	22.49209796014186
30	25.076644895883117	25.31894617110886	27.452986006891756	22.151422926116275
31	25.148189447737547	26.22275508008239	26.703445037854333	21.925610434325733
32	26.14114957562343	26.666554878304392	26.18027550241882	21.012020043653358
33	25.714118031742306	25.313915694806592	26.690589376192992	22.281376897258113
34	23.41155723983355	26.232816032686916	28.976661384666546	21.378965342812986
35	22.130183137284494	27.86827977273426	27.718762838194728	22.28277425178652
36	25.383503950321252	28.11002210614864	25.816683854127366	20.68979008940274
37	22.647204312795015	28.69188053177724	27.035177002898113	21.62573815252963
38	23.51076941135043	27.978950251384077	26.155402591813182	22.35487774545231
39	23.756983279255714	26.98878483255501	26.329512966052672	22.924718922136613
40	24.642067637548593	25.149027860454588	28.312359041861946	21.896545460134874
41	21.050587028637384	26.426768841229787	27.218230446119406	25.304413684013426
42	22.325812771261447	25.603168082186805	28.879684980395115	23.191334166156633
43	22.869942624623064	25.98576375206459	27.672091196945942	23.472202426366405
44	22.65838314902227	25.131700664302343	27.39122293673617	24.818693249939212
45	22.702819023025608	24.48556393036703	26.88007065024496	25.93154639636241
46	23.82210000027947	25.404743739153034	27.562538601918845	23.210617658648648
47	21.058971155807825	26.166301957134753	29.632300129395027	23.142426757662392
48	21.5826996330547	26.103141532450763	28.53006687738773	23.784091957106806
49	23.49204486066978	24.248293131443553	30.016852095612585	22.242809912274083
50	21.93343561968481	24.488358639423843	29.075035143466387	24.503170597424955
51	21.674086619212506	25.025781191049106	27.07402345878782	26.226108730950564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	300.0
2	454.0
3	1130.0
4	1806.0
5	1233.0
6	660.0
7	644.5
8	629.0
9	616.0
10	603.0
11	641.0
12	679.0
13	698.0
14	717.0
15	782.0
16	847.0
17	817.5
18	788.0
19	764.5
20	741.0
21	859.5
22	978.0
23	1148.5
24	1319.0
25	1716.0
26	2690.0
27	3267.0
28	3626.5
29	3986.0
30	4591.5
31	5197.0
32	6089.5
33	6982.0
34	8292.0
35	9602.0
36	10211.0
37	10820.0
38	11904.0
39	12988.0
40	14223.0
41	15458.0
42	17530.5
43	19603.0
44	21484.5
45	23366.0
46	27541.0
47	31716.0
48	33098.5
49	34481.0
50	34783.5
51	35086.0
52	31441.0
53	27796.0
54	25356.5
55	22917.0
56	21022.0
57	19127.0
58	18111.5
59	17096.0
60	14974.0
61	12852.0
62	11676.5
63	10501.0
64	9008.5
65	7516.0
66	6442.5
67	5369.0
68	4662.5
69	3956.0
70	3048.0
71	2140.0
72	1747.0
73	1354.0
74	1188.5
75	771.5
76	520.0
77	441.0
78	362.0
79	248.5
80	135.0
81	100.0
82	65.0
83	43.5
84	22.0
85	19.5
86	17.0
87	12.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	4.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.39324540513414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05200382981347	28.777555142046406
2	9.408299138856698	7.412468736437881
3	3.6264948257570824	4.285782018944937
4	2.1499552578280268	3.3877486032671156
5	1.5996782456241123	3.150825884956255
6	1.3165168543264252	3.111712291348566
7	1.0766031525743331	2.968762453531124
8	0.9282502689409204	2.9253432513417157
9	0.8317396060308495	2.9488430172188553
>10	5.935569538613707	36.35935457576592
>50	0.04921295650265526	1.3219400335772709
>100	0.01997047510252677	1.3556558261445977
>500	0.004279387521970022	1.3199732790108436
>1k	0.0014264625073233408	0.6740348864084745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1260	0.3521333411585187	Illumina PCR Primer Index 9 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	1139	0.31831736157107365	RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.27527884209614356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	887	0.24789069333936992	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	835	0.233358206243939	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	765	0.21379524284624352	RNA PCR Primer, Index 17 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	665	0.1858481522781071	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	561	0.15678317808724523	RNA PCR Primer, Index 17 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	381	0.10647841506459971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.384127170440921E-4	0.0	0.0	0.1640494216349607	0.0
2	8.384127170440921E-4	0.0	0.0	0.9021320835394431	0.0
3	8.384127170440921E-4	0.0	0.0	1.2193315614877913	0.0
4	8.384127170440921E-4	0.0	0.0	1.6692797196347875	0.0
5	8.384127170440921E-4	0.0	0.0	3.057132237248441	0.0
6	8.384127170440921E-4	0.0	0.0	3.7851539465483945	0.0
7	8.384127170440921E-4	0.0	0.0	4.4768444381097705	0.0
8	8.384127170440921E-4	0.0	0.0	5.527375572566018	0.0
9	8.384127170440921E-4	0.0	0.0	5.905779178858585	0.0
10	8.384127170440921E-4	0.0	0.0	7.008571372677247	0.0
11	8.384127170440921E-4	0.0	0.0	8.270382511828606	0.0
12	8.384127170440921E-4	0.0	0.0	9.464841162710757	0.0
13	8.384127170440921E-4	0.0	0.0	9.939662231463393	0.0
14	8.384127170440921E-4	0.0	0.0	10.158487950611901	0.0
15	8.384127170440921E-4	0.0	0.0	10.517049122601092	0.0
16	8.384127170440921E-4	0.0	0.0	11.18246934902842	0.0
17	8.384127170440921E-4	0.0	0.0	11.975607779352131	0.0
18	8.384127170440921E-4	0.0	0.0	12.8503517141348	0.0
19	8.384127170440921E-4	0.0	0.0	13.415162414516837	0.0
20	8.384127170440921E-4	0.0	0.0	13.943082955348933	0.0
21	8.384127170440921E-4	0.0	0.0	14.557360006036571	0.0
22	8.384127170440921E-4	0.0	0.0	15.219706052501405	0.0
23	8.384127170440921E-4	0.0	0.0	15.850471886624243	0.0
24	8.384127170440921E-4	0.0	0.0	16.346532744208663	0.0
25	8.384127170440921E-4	0.0	0.0	16.826663760169247	0.0
26	8.384127170440921E-4	0.0	0.0	17.303161654355975	0.0
27	8.384127170440921E-4	0.0	0.0	17.814872882658552	0.0
28	8.384127170440921E-4	0.0	0.0	18.286619771448695	0.0
29	8.384127170440921E-4	0.0	0.0	18.79581576160014	0.0
30	8.384127170440921E-4	0.0	0.0	19.442231966441135	0.0
31	8.384127170440921E-4	0.0	0.0	19.931864993194882	0.0
32	8.384127170440921E-4	0.0	0.0	20.421777490854314	0.0
33	8.384127170440921E-4	0.0	0.0	20.917279406627372	0.0
34	8.384127170440921E-4	0.0	0.0	21.449950952856053	0.0
35	8.384127170440921E-4	0.0	0.0	21.980945673650645	0.0
36	8.384127170440921E-4	0.0	0.0	22.450736266101018	0.0
37	8.384127170440921E-4	0.0	0.0	22.92415998032525	0.0
38	8.384127170440921E-4	0.0	0.0	23.479189199008438	0.0
39	8.384127170440921E-4	0.0	0.0	24.32011715420366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGT	25	3.8854036E-5	45.0	4
TAAGTCG	20	7.0267665E-4	45.0	1
CCAACGG	35	1.2087912E-7	45.0	2
ATACGAG	20	7.0267665E-4	45.0	1
GCGTACG	20	7.0267665E-4	45.0	1
GATAGCG	20	7.0267665E-4	45.0	1
AGCGACT	30	2.161296E-6	44.999996	19
CGTTTTT	650	0.0	40.84615	1
CGTGCGG	45	1.9219442E-8	40.000004	2
GGCGTAG	45	1.9219442E-8	40.000004	1
TAGCGCG	40	3.4493314E-7	39.375	1
TAACGGG	80	0.0	39.375	3
CGACAGG	35	6.236234E-6	38.571426	2
CTACGAA	70	0.0	38.571426	11
TACGAAT	70	0.0	38.571426	12
GCTACGA	70	0.0	38.571426	10
TCCAACG	30	1.1383512E-4	37.499996	1
ACGTAGG	30	1.1383512E-4	37.499996	2
AACGGGA	115	0.0	37.173916	4
CACGCCG	85	0.0	37.058826	26
>>END_MODULE